Molecular systematic studies on mandarina silkworm (Bombyx mandarina M.) in 11 regions in China and 25 representative strains of domestic silkworm (Bombyx mori L.) were conducted using molecular biology techniques. Re...Molecular systematic studies on mandarina silkworm (Bombyx mandarina M.) in 11 regions in China and 25 representative strains of domestic silkworm (Bombyx mori L.) were conducted using molecular biology techniques. Results obtained from the analysis of DNA polymorphism and clustering of all the silkworm samples provide new evidence for the view that the domestic silkworm originated from the Chinese mandarina silkworm. On the basis of literature reviewing, a new hypothesis on the origin of the domestic silkworm was put forward. It was thought that the domestic silkworm was most probably domesticated from the Chinese mandarina silkworm of different ecotypes including monovoltinism, bivoltinism and multivoltinism; and that the domestic silkworm had the genetic background of monovoltinism, bivoltinism and multivoltinism at the very beginning of the domestication. The current strains of the domestic silkworm of different voltinism are the evolutionary results of thousands of years of rearing and artificial selections.展开更多
[Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. [Method] The midgut tissue-specific cDNA library of wild silkworm was constructed v...[Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. [Method] The midgut tissue-specific cDNA library of wild silkworm was constructed via cDNA Library Construction Kit (TaKaRa), then the serine protease gene was cloned via sequencing of the yielded cDNA library. [Result] The titer of cDNA library reached 6.2×105 pfu/ml, average insert size was about 1.2 kb. The serine protease gene cDNA fragment was obtained from colony sequencing (Accession No: EU672968). The nucleotide sequence of the cloned 854 bp fragment encodes 284 amino acid residues. Homology analyses showed some homology between putative amino acid sequence of the cloned fragment and amino acid sequences of serine proteases from other ten insects. [Conclusion] The results may avail to reveal the resistance of silkworm and wild silkworm to exotic intrusion.展开更多
[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm （ Bombyx Mandarina）, thus providing the basis for scie...[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm （ Bombyx Mandarina）, thus providing the basis for scientific pesticide application and resistance management. [ Method] The wild silkworms collected from three different regions were reared indoors, and the sensitivity of their parents to deltamethrin was detected by topical application. The larvae in each generation were treated with deltamethdn in median lethal dose or so by.topical application. The mortality of larva was analyzed for the establishment of toxicity regression equations and the calculation of the multiple or increased multiple of deltamethdn resistance.[Result] After the Qidong Bornbyx Mandarina （YQD） fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethdn resistance of F, was 14.26, 1.2 times as great as that of Fo ; after the Bombyx Mandarina from mulberry garden of Soochow University （YSD） fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethrin resistance of F4 was 16.48, 1.9 times as great as that of Fo ; after the Wujiang Bombyx Mandarina （YWJ） fed with artificial diets were selected for three generations indoors during six generations, the multiple of deltamethrin resistance of Fe was 18.67, 1.2 times as great as that of Fo. [ Conclusioa] With the selection in same dose, the resistance multiple of YSD increases more rapidly than that of YQD; under double selection of artificial diets and insecticide, the resistance multiple of YWJ increases more slowly than that of YQD.展开更多
Diapause eggs of Bombyx mandarina Moore from Wujiang, Jiangsu Province, China, were used to study the artificial hatching of B. mandarina Moore. The results showed that the highest hatchability was obtained by instant...Diapause eggs of Bombyx mandarina Moore from Wujiang, Jiangsu Province, China, were used to study the artificial hatching of B. mandarina Moore. The results showed that the highest hatchability was obtained by instant treatment with hydrochloric acid （HC1, specific gravity 1.065-1.075） for 5 rain under 46℃. After the B. mandarina eggs were cold stored at 5℃ for 40 days, the highest hatchability was obtained by treatment with HC1 （specific gravity 1.092） for 6 minutes under 47.8℃. For the B. mandarina eggs that were stored at 25℃ for 28 d and then cold-stored at 5℃ for 0-100 days, the highest hatchability was obtained by treatment with HCI （specific gravity 1.092） for 6 rain at 47.8℃. The longer the cold storage period, the higher was the hatchability. Acid treatment on diapause eggs of B. mandarina for 6 rains at 47.8℃ with hydrochloric acid （specific gravity 1.092） before hatching in spring could obviously shorten the hatching stage and increase the hatchability.展开更多
将家蚕(Bombyxmori)和野桑蚕(Bombyxmandarina)杂交培育多代后发现并分离出一个遗传不育的自然突变系统,定名为镇江野败szm(Mandarina Sterility of Zhenjiang)。该系统表现为95%不育卵、3%可育卵和2%中间型,以3%可育卵继代,后代雌、雄...将家蚕(Bombyxmori)和野桑蚕(Bombyxmandarina)杂交培育多代后发现并分离出一个遗传不育的自然突变系统,定名为镇江野败szm(Mandarina Sterility of Zhenjiang)。该系统表现为95%不育卵、3%可育卵和2%中间型,以3%可育卵继代,后代雌、雄比例正常,性状稳定遗传。经遗传分析,该突变性状对正常表现为隐性。经组织解剖证实其产生原因可能是雄性精液分泌物失调、精荚异常、雄性外生殖器异常等。展开更多
基金supported by the National Natural Science Foundation of China(No.39870603)the Special Funding Program of the Ministry of Education for Ph.D.Degree Awarding Units.
文摘Molecular systematic studies on mandarina silkworm (Bombyx mandarina M.) in 11 regions in China and 25 representative strains of domestic silkworm (Bombyx mori L.) were conducted using molecular biology techniques. Results obtained from the analysis of DNA polymorphism and clustering of all the silkworm samples provide new evidence for the view that the domestic silkworm originated from the Chinese mandarina silkworm. On the basis of literature reviewing, a new hypothesis on the origin of the domestic silkworm was put forward. It was thought that the domestic silkworm was most probably domesticated from the Chinese mandarina silkworm of different ecotypes including monovoltinism, bivoltinism and multivoltinism; and that the domestic silkworm had the genetic background of monovoltinism, bivoltinism and multivoltinism at the very beginning of the domestication. The current strains of the domestic silkworm of different voltinism are the evolutionary results of thousands of years of rearing and artificial selections.
文摘[Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. [Method] The midgut tissue-specific cDNA library of wild silkworm was constructed via cDNA Library Construction Kit (TaKaRa), then the serine protease gene was cloned via sequencing of the yielded cDNA library. [Result] The titer of cDNA library reached 6.2×105 pfu/ml, average insert size was about 1.2 kb. The serine protease gene cDNA fragment was obtained from colony sequencing (Accession No: EU672968). The nucleotide sequence of the cloned 854 bp fragment encodes 284 amino acid residues. Homology analyses showed some homology between putative amino acid sequence of the cloned fragment and amino acid sequences of serine proteases from other ten insects. [Conclusion] The results may avail to reveal the resistance of silkworm and wild silkworm to exotic intrusion.
文摘[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm （ Bombyx Mandarina）, thus providing the basis for scientific pesticide application and resistance management. [ Method] The wild silkworms collected from three different regions were reared indoors, and the sensitivity of their parents to deltamethrin was detected by topical application. The larvae in each generation were treated with deltamethdn in median lethal dose or so by.topical application. The mortality of larva was analyzed for the establishment of toxicity regression equations and the calculation of the multiple or increased multiple of deltamethdn resistance.[Result] After the Qidong Bornbyx Mandarina （YQD） fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethdn resistance of F, was 14.26, 1.2 times as great as that of Fo ; after the Bombyx Mandarina from mulberry garden of Soochow University （YSD） fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethrin resistance of F4 was 16.48, 1.9 times as great as that of Fo ; after the Wujiang Bombyx Mandarina （YWJ） fed with artificial diets were selected for three generations indoors during six generations, the multiple of deltamethrin resistance of Fe was 18.67, 1.2 times as great as that of Fo. [ Conclusioa] With the selection in same dose, the resistance multiple of YSD increases more rapidly than that of YQD; under double selection of artificial diets and insecticide, the resistance multiple of YWJ increases more slowly than that of YQD.
基金supported by the National Natural Science Foundation of China(30471309)the National Basic Research Program of China(2005CB121005).
文摘Diapause eggs of Bombyx mandarina Moore from Wujiang, Jiangsu Province, China, were used to study the artificial hatching of B. mandarina Moore. The results showed that the highest hatchability was obtained by instant treatment with hydrochloric acid （HC1, specific gravity 1.065-1.075） for 5 rain under 46℃. After the B. mandarina eggs were cold stored at 5℃ for 40 days, the highest hatchability was obtained by treatment with HC1 （specific gravity 1.092） for 6 minutes under 47.8℃. For the B. mandarina eggs that were stored at 25℃ for 28 d and then cold-stored at 5℃ for 0-100 days, the highest hatchability was obtained by treatment with HCI （specific gravity 1.092） for 6 rain at 47.8℃. The longer the cold storage period, the higher was the hatchability. Acid treatment on diapause eggs of B. mandarina for 6 rains at 47.8℃ with hydrochloric acid （specific gravity 1.092） before hatching in spring could obviously shorten the hatching stage and increase the hatchability.
文摘将家蚕(Bombyxmori)和野桑蚕(Bombyxmandarina)杂交培育多代后发现并分离出一个遗传不育的自然突变系统,定名为镇江野败szm(Mandarina Sterility of Zhenjiang)。该系统表现为95%不育卵、3%可育卵和2%中间型,以3%可育卵继代,后代雌、雄比例正常,性状稳定遗传。经遗传分析,该突变性状对正常表现为隐性。经组织解剖证实其产生原因可能是雄性精液分泌物失调、精荚异常、雄性外生殖器异常等。
