OBJECTIVE: To investigate the manifestation of clinical, blood routine and bone marrow smear of disseminated penicilliosis marneffei. METHODS: There were 13 cases of penicilliosis marneffei whose peripheral blood had ...OBJECTIVE: To investigate the manifestation of clinical, blood routine and bone marrow smear of disseminated penicilliosis marneffei. METHODS: There were 13 cases of penicilliosis marneffei whose peripheral blood had been drawn for routine tests, as well as blood and bone marrow aspiration for smears. Wright's-Giemsa stain, Gomori's methenamine-silver stain (GMS) and periodic acid Schiff's reaction (PAS) were performed for light microscopy in consultation with pathologic diagnosis and fungi culture for precise diagnosis. RESULTS: Seven cases of bone marrow and 2 peripheral blood smears were found positive for penicillium marneffei in the test group. The morphology of penicillium marneffei was extremely similar to that of histoplasma capsulatum. However, the observation of sausage cells and central cross wall, which are signs of dividing by fission and not by budding, aided in differential diagnosis. CONCLUSION: Bone marrow smear or occasional blood smear examination play an important role in the diagnosis of disseminated penicilliosis marneffei.展开更多
At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gain...At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention.Here,we report the effects of prolonged peroral administration of GNPs with different sizes(2,15 and 50 nm)on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals.The experiment was conducted on 24 white mongrel male rats weighing 180–220 g,gold nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosage of 190g/kg of animal body weight.The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability.The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of GNPs with size of 50 nm.The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow.The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing number of immunoblasts and large lymphocytes.The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of GNPs.展开更多
文摘OBJECTIVE: To investigate the manifestation of clinical, blood routine and bone marrow smear of disseminated penicilliosis marneffei. METHODS: There were 13 cases of penicilliosis marneffei whose peripheral blood had been drawn for routine tests, as well as blood and bone marrow aspiration for smears. Wright's-Giemsa stain, Gomori's methenamine-silver stain (GMS) and periodic acid Schiff's reaction (PAS) were performed for light microscopy in consultation with pathologic diagnosis and fungi culture for precise diagnosis. RESULTS: Seven cases of bone marrow and 2 peripheral blood smears were found positive for penicillium marneffei in the test group. The morphology of penicillium marneffei was extremely similar to that of histoplasma capsulatum. However, the observation of sausage cells and central cross wall, which are signs of dividing by fission and not by budding, aided in differential diagnosis. CONCLUSION: Bone marrow smear or occasional blood smear examination play an important role in the diagnosis of disseminated penicilliosis marneffei.
文摘At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention.Here,we report the effects of prolonged peroral administration of GNPs with different sizes(2,15 and 50 nm)on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals.The experiment was conducted on 24 white mongrel male rats weighing 180–220 g,gold nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosage of 190g/kg of animal body weight.The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability.The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of GNPs with size of 50 nm.The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow.The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing number of immunoblasts and large lymphocytes.The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of GNPs.
