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Localized Effects of Mechanical Wounding and Exogenous Jasmonic Acid on the Induction of Secondary Laticifer Differentiation in Relation to the Distribution of Jasmonic Acid in Hevea brasiliensis 被引量:14
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作者 田维敏 史敏晶 +3 位作者 于凤义 吴继林 郝秉中 崔克明 《Acta Botanica Sinica》 CSCD 2003年第11期1366-1372,共7页
The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated ... The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated with the aid of experimental morphological and radioisotope technique. Most radioactivity of H-3-JA sustained in treated site within one hour while no radioactivity was detected in new shoot and the radioactivity in upper leaf was much less than that in the parts below the treated site, suggesting that JA was mainly transported downwards in the shoot of H brasiliensis. Mechanical wounding hindered the entrance of exogenous JA remarkably while held back the entered JA to the regions around wounded site. The effect of exogenous JA and mechanical wounding on the induction of the secondary laticifer differentiation was limited to treated site where high level of JA was expected. Mechanical wounding reduced the effect of exogenous JA on the differentiation of secondary laticifer, which could be ascribed to the hindrance of mechanical wounding to the entrance of exogenous JA. It was concluded from the combined data that a high accumulation of JA was required for inducing the secondary laticifer differentiation in H. brasiliensis. 展开更多
关键词 Hevea brasiliensis jasmonic acid mechanical wounding radioisotope technique laticifer differentiation
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AFLP Fingerprinting Analysis of Elite Hevea brasiliensis Germplasm 被引量:22
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作者 罗安定 陈守才 +1 位作者 吴坤鑫 符少萍 《Acta Botanica Sinica》 CSCD 2001年第9期941-947,共7页
There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated b... There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated by production and previous studies. AFLP (amplified fragment length polymorphism) fingerprinting analysis was performed on 25 clones (15 of Wickham clones and 10 of Amazon wild clones which possess phenotypes with high-yielding,/Iow-yielding, cold-tolerance/cold-sensitivity, oidium-resistance/oidium-sensitivity, tapping panel dryness (TPD) /healthy) respectively through a 377 DNA sequencer (P. E. Corp.) and PAGE electrophoresis results were analyzed by using GeneScan(TM) and Genotype(TM) Analysis software (P. E. Corp. The fragment profiles of different clones were obtained. Five hundred and eighteen fragments were generated by two primer combinations screened from 64 primer combinations and 511 fragments appeared to be polymorphic (98.6%). Genetic distance ranged from 0.25 to 0.81 between clones and ranged from 0.07 to 0.17 within RRIM600 clone. A specific 320 bp fragment of the oidium-resistant clones was found through genotype analysis. These results showed that AFLP fingerprints were highly reproducible and powerful and can be widely used in germplasm identification and genetic diversity analysis of Hema brasiliensis. In addition, based on the AFLP data, cluster analysis was performed. Cluster results showed that all the clones studied were almost clustered into a group one by one. 展开更多
关键词 Hema brasiliensis AFLP germplasm identification genetic diversity
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Fluctuation of Microfibrillar Protein Level in Lutoids of Primary Laticifers in Relation to the 67 kD Storage Protein in Hevea brasiliensis 被引量:1
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作者 田维敏 韩亚琴 +1 位作者 吴继林 胡正海 《Acta Botanica Sinica》 CSCD 2003年第2期127-130,共4页
Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each ... Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each in separate lutoids. As revealed by SDS-PAGE, the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoeleettic point precipitation. Western-blotting analysis indicated that they were immunorelated with the 67 kD protein accumulated in the protein-storing cells. The 59.5 kD and 63.5 kD proteins were abundant in the uppermost part, the stem of new shoot and sustained their abundance during the growth and development of new shoot while their contents decreased remarkably in the lower parts of the trunk, accompanying by the accumulation of 3-5 kinds of proteins with low molecular weights. This fluctuating pattern suggested that the degradation of the 59.5 kD and 63.5 kD proteins had nothing to do with the new shoot growth and may be closely related to the primary laticifer differentiation. The 67 kD protein could not be detected in the young stem of new shoot when its leaves were broze-colored while the protein started to be accumulated in the stem, when the leaves of new shoot had matured, behaving like a typical vegetative storage protein. 展开更多
关键词 microfibrillar protein 67 kD protein shoot growth laticifer development Hevea brasiliensis
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Effects of Micro-tapping with Different Concentration of Ethylene on Yield and Physiological Features of Rubber Tree(Hevea brasiliensis) Clone PR107
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作者 仇键 杨文凤 +3 位作者 吴明 魏芳 校现周 罗世巧 《Agricultural Science & Technology》 CAS 2015年第5期873-876,935,共5页
In this study, the rubber tree clone PR107 was selected as test material,and its yields and physiological features under micro-tapping with different concentrations of ethylene were compared. The results showed that t... In this study, the rubber tree clone PR107 was selected as test material,and its yields and physiological features under micro-tapping with different concentrations of ethylene were compared. The results showed that there was no significant correlation between rubber yield and ethylene concentration applied by microtapping on PR107. Too high level of ethylene was not conducive to improvement of rubber yield. When the ethylene concentration for micro-tapping was higher than60%, physical deficiencies appeared in rubber tree PR107, the dry rubber content was reduced, and the yield was unstable. Instead, when the ethylene concentration was lower than 40%, the physiological features of PR107 were steady, the dry rubber content was stable, and the yield was relatively high. 展开更多
关键词 Micro-tapping Physiological features of latex Ethylene concentration Hevea brasiliensis PR107
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Study on the Explant Disinfection of Root Culture of Hevea brasiliensis
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作者 姜泽海 周权男 李哲 《Agricultural Science & Technology》 CAS 2011年第4期514-516,共3页
[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots fro... [Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots from forest section after treatment for one month were as explants,and 1 g/L carbendazim,75% ethanol,0.1% mercuric chloride and Yipeilong with different concentrations were used as disinfectants for roots disinfection.[Result] Before conventional disinfection,root explants were treated by 1 g/L carbendazim for 2.5 h,and disinfected by 75% ethanol for 30 s and 0.1% HgCl2 for 6 min,then cultured on the callus induction medium supplemented with 0.1% Yipeilong.The results showed that the contamination rate of explants decreased to 44.59%,and 25.60% explants survived after cultured for 30 d.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H.brasiliensis. 展开更多
关键词 Hevea brasiliensis Root explants Contamination control CARBENDAZIM Yipeilong
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Molecular Cloning and Bioinformatical Analysis of a cDNA Encoding Mitochondrial 50S Ribosomal Protein L21 from Hevea brasiliensis Muell. Arg.
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作者 邹智 杨礼富 《Agricultural Science & Technology》 CAS 2012年第6期1203-1206,共4页
[Objective] "Tapping panel dryness (TPD)", a syndrome known as tapping incision blocked partly or entirely during latex exploiting, has become the most important factor causing great losses for rubber production. ... [Objective] "Tapping panel dryness (TPD)", a syndrome known as tapping incision blocked partly or entirely during latex exploiting, has become the most important factor causing great losses for rubber production. Aiming to elucidate the molecular mechanism of tapping panel dryness occurrence, this study carried out molecular cloning and bioinformatical analysis of a mRPL21 cDNA sequence, a gene associated with TPD. [Method] In a preliminary study, an expressed sequence tag (EST) encoding a deduced protein homologous to mitochondrial 50S ribosomal protein L21 (mRPL21), which showed to be down-regulated in the latex of TPD-affected rubber trees, was isolated by suppression subtractive hybridization (SSH). After ESTs assembling and RT-PCR validation, an 853 bp cDNA sequence with an open reading frame (ORF) was cloned, which was named as HbmRPL21 under GenBank accession number of HM230670. [Result] Bioinformatical analysis suggests that HbmRPL21 encodes a deduced polypeptide of 271 amino acids with a theoretical molecular weight (Mw) of 30.52 kDa and isolectric point (pI) of 8.40, and HbmRPL21 is a mitochondrion-targeted protein with a conserved domain of Ribosomal_L21p involving translation. Homology analysis reveals high amino acid sequence identity of mRPL21 from plants, while diversity of that between plant and animal kingdom. [Conclusion] This study laid the basis for further revealing the biological functions of mRPL21 in TPD-affected rubber trees. 展开更多
关键词 Hevea brasiliensis Tapping panel dryness syndrome Mitochondrial 50S ribosomal protein L21
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Evaluation of unconstrained and constrained mathematical functions to model girth growth of rubber trees (Hevea brasiliensis) using young agemeasurements 被引量:6
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作者 T.R.Chandrasekhar 《Journal of Forestry Research》 CAS CSCD 2012年第3期365-375,共11页
No attempt has been made to date to model growth in girth of rubber tree (Hevea brasiliansis). We evaluated the few widely used growth functions to identify the most parsimonious and biologically reasonable model fo... No attempt has been made to date to model growth in girth of rubber tree (Hevea brasiliansis). We evaluated the few widely used growth functions to identify the most parsimonious and biologically reasonable model for describing the girth growth of young rubber trees based on an incomplete set of young age measurements. Monthly data for girth of immature trees (age 2 to 12 yearsi from two locations were sub- jected to modelling. Re-parameterized, unconstrained and constrained growth functions,of Richards (RM), Gompertz (GM) and the monomo- lecular 'model ^(MM) were fitted to data. Duration of growth was the firsf constraint introduced. In the stagel We attempted a population aver- age (PA) model to capture the trend in growth. The best PA model was fitted as a subject specific (SS) model. We used appropriate error vari- ance-covariance structure to account for correlation due to repeated measurements over time. Unconstrainecl functions underestimated the asymptotic maximum that did not reflective carrying capacity of the locations. Underestimafions were attributed to the partial set' of meas- urements made during the early growth phase of the trees. MM proved superior to RM and GM. In the randomcoefficient models, both Gf and Go appeared to be influenced by tree level effects. Inclusion of diagonal definite positive matrix removed the correlation between random effects. The results were similar at both locations. In the overall assessment MM appeared as the candidate model for studying the girth-age relationships in Hevea trees. Based on the fitted model we conclude that, in Hevea trees, growth rate is maintained at maximum value at to, then decreases until the final state at dG/dt 〉 0, resulting in yield curve with no period of accelerating growth. One physiological explanation is that photosynthetic activity in Hevea trees decreases as girth increases and constructive metabolism is larger than destructive metabolism. 展开更多
关键词 natural rubber Hevea brasiliensis growth modelling un-constrained functions constrained functions mixed model
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Preliminary Study on Seedling Growth Rhythm and Grey Correlation Analysis of Rubber (Hevea brasiliensis) Seedlings in Danzhou District, Hainan 被引量:5
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作者 Dongling Qi Chuan Yang +2 位作者 Guishui Xie Zhixiang Wu Zhixiang Wu 《American Journal of Plant Sciences》 2014年第26期3866-3872,共7页
The rubber tree physiological and ecological process quantitatively described by using mathematical method is an important means to the analysis of rubber tree growth process and mechanism. The study on growth simulat... The rubber tree physiological and ecological process quantitatively described by using mathematical method is an important means to the analysis of rubber tree growth process and mechanism. The study on growth simulation model of rubber tree will lay the foundation for the application of rubber tree cultivation intelligent decision system. A Richards equation was formulated to describe the height and stem diameter growth dynamics of the annual rubber seedlings. An area correlation analysis was done according to the closeness of the observed parameters to the dynamic curve on the gray system composed of the seedling growth increment and the meteorological factors including aerial temperature, precipitation and solar radiation hours that influence upon the seedling growth. The results showed that rubber seedling response fitted the Richards equation quite well. The growth increment displayed a distinct alternation of 'slow—fast—slow— fast—slow' rhythm. The growth course of the seedlings might be partitioned into three periods of time by the sequential clustering analysis, namely pre-growing, fast-growing, late-growing stage. The tray correlation analysis revealed that air temperature had the most significant influence while precipitation had the least impact on height growth of the rubber seedlings. In conclusion, the air temperature had the most significant influence while solar radiation hours had the least impact on stem diameter growth of the rubber seedlings. 展开更多
关键词 RUBBER (Hevea brasiliensis) RICHARDS Equation Growth RHYTHM Gray Correlation
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Morphological Studies on the Infraciliature of a Planktonic Ciliate,Tintinnopsis brasiliensis(Ciliophora:Tintinina) 被引量:1
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作者 CAI Shengfang SONG Weibo +1 位作者 XU Dapeng CHIANG Kuoping 《Journal of Ocean University of China》 SCIE CAS 2006年第1期55-57,共3页
A poorly-described marine planktonic ciliate, Tintinnopsis brasiliensis Kofoid & Campbell, 1929, collected from the Taiping Cape of Qingdao, China, was morphologically investigated based on permanent preparation afte... A poorly-described marine planktonic ciliate, Tintinnopsis brasiliensis Kofoid & Campbell, 1929, collected from the Taiping Cape of Qingdao, China, was morphologically investigated based on permanent preparation after protargol impregnation and was compared with other related congeners. According to the infraciliature, three ciliary groups can be recognized, which reveals a very stable structure among specimens and denotes that the pattern of infraciliature is, apart from the features of the lorica, a highly reliable criterion for species identification. 展开更多
关键词 INFRACILIATURE Tintinnina CILIOPHORA Tintinnopsis brasiliensis
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Effect of natural and synthetic antioxidants on oxidative stability of FAMEs obtained from hevea brasiliensis 被引量:1
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作者 S.Nivetha D.Vetha Roy 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2013年第6期935-941,共7页
Biodiesel(fatty acid alkyl esters),an alternate to fossil fuel,has the tendency of autoxidation and hence requires antioxidants for long term storage.The influence of synthetic and natural antioxidants on the oxidativ... Biodiesel(fatty acid alkyl esters),an alternate to fossil fuel,has the tendency of autoxidation and hence requires antioxidants for long term storage.The influence of synthetic and natural antioxidants on the oxidative stability was analysed for fresh FAMEs(fatty acid methyl esters)obtained from hevea brasiliensis at 140 C.Higher activity was observed for synthetic antioxidants following the order of GA】BHT】DTBP】Q】GT-M】PH-M】GT-C,whereas the oxidative stability of stored FAMEs samples measured at 110 C reveals a nearly inverse trend.Storage stability was tested for the FAMEs obtained from hevea brasiliensis stored at 30 C,after addition of synthetic and natural antioxidants—butylated hydroxytoluene(BHT),2,6-di-tert-butylphenol(DTBP),quercetin(Q),gallic acid(GA),methanol extracts from green tea(GTM),pomegranate hull(PH-M),and chloroform extract of green tea(GT-C).Antioxidant activities above 1500 ppm was in the order of DTBP】BHT】GA】GT-C】GT-M】Q】PH-M.Synthetic antioxidants have been found more efficient to improve the storage stability of FAMEs obtained from hevea brasiliensis.DTBP in particular has the highest protection factor. 展开更多
关键词 hevea brasiliensis fatty acid methyl esters oxidative stability storage stability ANTIOXIDANTS
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Proximate Composition and Fatty Acid Profile of Raw and Roasted Salt-Dried Sardines (<i>Sardinella Brasiliensis</i>) 被引量:1
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作者 Milena Keller Bulla Julliana Isabelle Simionato +4 位作者 Makoto Matsushita Fabio Augusto Garcia Coró Massami Shimokomaki Jesuí Vergilio Visentainer Nilson Evelazio de Souza 《Food and Nutrition Sciences》 2011年第5期440-443,共4页
The proximate composition and fatty acid profile of five lots of samples of raw and roasted salt-dried sardines (Sardi-nella brasiliensis) bought locally in Maringá, Paraná State, Brazil were determined. Sig... The proximate composition and fatty acid profile of five lots of samples of raw and roasted salt-dried sardines (Sardi-nella brasiliensis) bought locally in Maringá, Paraná State, Brazil were determined. Significant differences (P < 0.05) were observed between samples of raw and roasted sardines, both in relation to moisture, total lipids, proteins, and ashes. The major fatty acids in raw and roasted sardine samples were docosahexaenoic acid (DHA, 22: 6n-3, 35.98%, 12.46%);palmitic acid (16: 0, 37.59%;24.18%), and eicosapentaenoic acid (EPA, 20: 5n-3, 6.62%;2.95%), respec-tively. The ratios of polyunsaturated to saturated fatty acid (PUFA/SFA) were 1.32 and 0.33, and the n-6/n-3 ratios were 0.07 and 0.13 in raw and roasted sardines, respectively. The results showed that roasting increased the SFA and reduced the PUFA in sardines, which still were rich in PUFA and remained a low-cost and nutritionally healthy food. 展开更多
关键词 SARDINE Sardinella brasiliensis RAW SARDINE Roasted SARDINE Fatty Acids
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Molecular Cloning and Analysis of LEA14L2 Gene from Rubber Tree( Hevea brasiliensis Muell. Arg. ) 被引量:1
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作者 Zhi ZOU Lifu YANG +2 位作者 Feng AN Zhenhui WANG Kun YUAN 《Agricultural Biotechnology》 CAS 2013年第5期4-8,共5页
Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query, a 613 bp long cDNA was in silico cloned from the transeriptome data of rubber tree. The sequence nominated as HbLEA... Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query, a 613 bp long cDNA was in silico cloned from the transeriptome data of rubber tree. The sequence nominated as HbLEA14L2 contains an ORF of 456 bp with 3 bp 5' UTR and 154 bp 3' UTR. Subsequently, a 464 bp eDNA and an 834 bp genome sequence containing this ORF was amplified and sequenced. Sequence analysis suggested that HbLEA14L2 has one intron and encodes 151 amino acids with a theoretical molecular weight of 16.55 kDa, isoleetric point of 4.93 and GRAVY value of -0.022, indicating a cytoplasmle localization pattern; HbLEA14L2 protein contains a conserved LEA_2 domain and belongs to LEA_2 subfamily, sharing 91%, 76%, 75%, 72% and 63% similarity with the homologous proteins in castor bean, leafy spurge, poplar, cotton, and Arabidopsis, respectively. Swiss-Model indicated that the tertiary structure of HbLEA14L2 is consisted of one α-helix and seven β-sheets, which was proposed to serve as a regulatory protein to prevent cellular desiccation. 展开更多
关键词 Hevea brasiliensis Muell. Arg. Arabidopsis thaliana LEA14 Shoot apex
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Corneal ulcer caused by nocardia brasiliensis in a patient with diabetes 被引量:1
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作者 Xian-Ning Liu Na An +3 位作者 Jie Wu Kun Xu Xiu-Ping Zh Sheng-Li Mi 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2014年第2期381-383,共3页
Dear Sir,Iam Dr.Sheng-Li Mi,from the Biomanufacturing engineering laboratory,Graduate School at Shenzhen,Tsinghua University,Shenzhen,China.I write to present a case report of nocardia brasiliensis in a patient with d... Dear Sir,Iam Dr.Sheng-Li Mi,from the Biomanufacturing engineering laboratory,Graduate School at Shenzhen,Tsinghua University,Shenzhen,China.I write to present a case report of nocardia brasiliensis in a patient with diabetes.Nocardia is part of a group of aerobic actinomycetes,widely distributed in soil.Nocardia asteroids and nocardia brasiliensis are the most common human pathogens.Humans infected by nocardia asteroids through the respiratory tract mainly suffer from primary suppurative pulmonary conditions.Infection of nocardia brasiliensis often occurs in the advanced stages of a progressive disease or immune disorder,especially Cushing syndrome,diabetes,or in patients using corticosteroids,immunosuppressive agents and broad-spectrum antibiotics for long time[1].To our knowledge,the reports of nocardia keratitis was very rare 展开更多
关键词 Corneal ulcer caused by nocardia brasiliensis in a patient with diabetes
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Intra-and inter-clonal tree growth variations of Hevea brasiliensis
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作者 H.R.Naji M.H.Sahri 《Journal of Forestry Research》 CAS CSCD 2012年第3期429-434,共6页
We evaluated the effects of planting densities (500, 1,000, 1,500 and 2,000 trees.ha-1) on tree growth performance (diameter at base, diameter at breast height, and clear bole height) of two clones (RRIM 2020 and... We evaluated the effects of planting densities (500, 1,000, 1,500 and 2,000 trees.ha-1) on tree growth performance (diameter at base, diameter at breast height, and clear bole height) of two clones (RRIM 2020 and PRIM 2025) of nine years old plantations of rubber tree (Hevea brasiliensis Muell. Arg) in Malaysia. For the four planting densities of the two clones, basal area and diameter at breast height declined with increasing planting density. Clear bole heights were greatest at 1,500 trees.haland lowest at 500 trees.ha-1 for the clone RRIM 2020, and at 2,000 trees.ha-1and 500 trees.halfor clone RRIM 2025. We conclude that the ideal planting density is 2,000 trees.ha"l for obtaining high volume of wood production and 500 trees-ha-1 for high wood quality. 展开更多
关键词 CLONE planting density tree growth Hevea brasiliensis
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De Novo Transcriptome Analysis in Hevea brasiliensis to Unveil Genes Involved in Low Temperature Stress Response
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作者 Bingsong Yang Xiaomeng Shi +1 位作者 Tingting Guo Meixia Liang 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第2期559-575,共17页
Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclea... Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclear.In this study,7,159 and 7,600 differentially expressed genes(DEGs)were identified in‘Reyan 73397’rubber trees.Through GO analysis,the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures(room temperature and 4°C,respectively),while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two temperatures.In addition,expression of 9 members of transcription factor MYB family genes were further verified by qRT-PCR,and MYB family genes may play important roles in the regulation of rubber trees under low temperature stress.This study provided a theoretical foundation for(1)revealing the molecular mechanisms of rubber trees in response to low temperature and(2)breeding of tolerant varieties of rubber trees. 展开更多
关键词 Hevea brasiliensis low temperature stress TRANSCRIPTOME differential expressed genes
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Habitat Preference and Effects of Coastal Fragmentation in the Sand-Dwelling Spider <i>Allocosa brasiliensis</i>(Lycosidae, Allocosinae)
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作者 Carolina Jorge álvaro Laborda +2 位作者 Marcelo Alves Días Anita Aisenberg Miguel Simó 《Open Journal of Animal Sciences》 2015年第3期309-324,共16页
Allocosa brasiliensis is a sand-dwelling wolf spider considered a good bioindicator to evaluate the quality of coastal dune ecosystems from Uruguay. Habitat fragmentation and human activities have impacted and reduced... Allocosa brasiliensis is a sand-dwelling wolf spider considered a good bioindicator to evaluate the quality of coastal dune ecosystems from Uruguay. Habitat fragmentation and human activities have impacted and reduced the Southern Uruguayan coast during the last decades. The aim of the present study was to evaluate the density, surface activity and habitat preference of A. brasiliensis in beaches with different human impact. For that purpose, we sampled during the night with head lamps and applied the capture-mark-recapture method. Females were more abundant than males and were the most recaptured sex. The most fragmented beaches showed lower number of burrows and individuals, especially in immatures stages. We found that the most suitable sandy habitat for A. brasiliensis should present a vegetation cover optimum of 25% - 50% of the surface. This study could provide tools for the implementation of future management conservation plans of the Southern Uruguayan coastline. 展开更多
关键词 Allocosa brasiliensis WOLF Spiders Sandy Coast BIOINDICATOR Ecology
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Immunophenotyping of peripheral blood mononuclear cells and intracellular detection of IL-2, IFNγ and IL-4 by flow cytometry in patients with actinomycetoma by Nocardia brasiliensis and Actinomadura madurae. Findings in six patients
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作者 Heuze de Icaza Yvonne Castrillón Rivera Laura Estela +4 位作者 Garibay-Escobar Adriana Santos-Argumedo Leopoldo Sandoval Trujillo Horacio Padilla Desgarennes Carmen Palma Ramos Alejandro 《Health》 2011年第3期172-178,共7页
Mycetoma is a chronic, granulomatous, progressive inflammatory disease;it usually involves the subcutaneous tissue after a traumatic inoculation of the causative organism. Mycetoma may be caused by true fungi or bacte... Mycetoma is a chronic, granulomatous, progressive inflammatory disease;it usually involves the subcutaneous tissue after a traumatic inoculation of the causative organism. Mycetoma may be caused by true fungi or bacteria (actinomycetes) and hence it is classified as eumycetoma and actinomycetoma respectively. Mycetoma immunological studies have fre- quently addressed humoral aspects. Few reports have addressed the role of cellular immunity in humans, for this reason, we were interested in finding differences in the circulating population of mononuclear cells or in the ability of this pathology to produce cytokines after mitogen stimulation. In this study, immunophenotyping of peripheral blood mononuclear cells (PBMN) and intracellular detection by flow cytometry of IL-2, IFNγ and IL-4 in patients with actino- mycetoma by Nocardia brasiliensis and Actino- madura madurae were evaluated. We investi- gated the expression of T-cells (CD3, CD4 and CD8), B-cells (CD19), monocytes (CD14), and natural killer cells (CD16 and CD56) markers. CD69 and CD25 were used to monitor individual activated cell subsets. The percentage values of the cells were calculated. Our results indicated that PBMC from patients with mycetoma show similar percentages of circulating cells when compared with healthy donors. The expression of IL-2 receptor (IL-2R) by mitogen activation was similar in these two groups. These findings suggest that circulating lymphocytes are not affected by this pathology. Intracellular IL-4 was increased only in patients with mycetoma by N. brasiliensis, suggesting a TH2 profile;this ob- servation has been reported by other authors. 展开更多
关键词 ACTINOMYCETOMA NOCARDIA brasiliensis Actinomadura madurae Cytokines CD4+ CD8+ CD56+
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Studies on Rubber (<i>Hevea brasiliensis</i>) Trees Exist Plant Type after Planting and Available Tapping Tree of Rubber Plantation in China
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作者 Dongling Qi Jiannan Zhou +1 位作者 Guishui Xie Zhixiang Wu 《American Journal of Plant Sciences》 2014年第20期3017-3021,共5页
Existing plant types of rubber tree after planting and available tapping tree were investigated, and there were about 28 rubber plantations with different tapping years of 8 varieties “CATAS7-33-97”, “CATAS8-79”, ... Existing plant types of rubber tree after planting and available tapping tree were investigated, and there were about 28 rubber plantations with different tapping years of 8 varieties “CATAS7-33-97”, “CATAS8-79”, “CATAS7-20-59”, “PR107”, “RRIM600”, “GT1”, “INA873”, “93-114”in South China. The results showed that there were six kinds of existing plant types of rubber tree after planting of rubber plantations, which were available tapping trees, wind damaged trees, cold damaged trees, tapping panel dryness trees, absent trees and weak trees, respectively. These data investigated also showed rubber trees under available tapping, stoppage due to tapping panel dryness, absence, wind damage, cold damage and weakness were counted and calculated and made up for 72.21%, 14.75%, 5.61%, 3.86%, 2.68% and 1.89%. Tapping panel dryness trees, wind damage and absent trees are major factors for the loss of tapping rubber trees in the rubber plantations. Of these investigated varieties, available tapping trees per 100 trees of rubber plantation of “PR107”at the 1st, 12th, 14th, 16th, 20th, 24th tapping year were 96, 67, 70, 75, 66, 46 trees in Hainan planting zone, respectively. Available tapping trees per 100 trees of rubber plantation of “RRIM600”at the 9th, 15th, 20th, 22nd tapping year were 88, 62, 55, 36 trees in Yunnan planting zone, respectively. Available tapping trees per 100 trees of rubber plantation of “93-114” at the 10th, 19th, tapping year were 94, 62 trees in Guangdong planting zone. These results showed that available tapping trees of rubber plantation decreased with increasing tapping age under different planting zones in China. 展开更多
关键词 RUBBER TREE (Hevea brasiliensis) Exist Plant Type Available TAPPING TREE RUBBER Plantations Different TAPPING Years China
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An Analysis of the Patents concerning Hevea brasiliensis
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作者 Xijuan LI Qing CHEN +3 位作者 Bingjiang XU Xinxin REN Huasun HUANG Qiong LI 《Asian Agricultural Research》 2015年第9期58-61 64,64,共5页
This paper mainly analyzes the application data of patents concerning Hevea brasiliensis in the world and China from the perspectives of time,research field,and the geographical distribution of applicants. It also int... This paper mainly analyzes the application data of patents concerning Hevea brasiliensis in the world and China from the perspectives of time,research field,and the geographical distribution of applicants. It also interprets the patents for invention in China from time,research field,and types of applicants. Based on the study,it shows that although the patent applications fluctuate,the general trend is rising. And the patents are mostly concentrated in plant protection,and agricultural biotechnology. The Chinese applicants of patents for invention largely work in the research institutes,and most of these patents focus on the field of plant protection,agricultural biotechnology,and agro-processing.However,the foreign applicants mainly work in companies,and most of these patents only focus on plant protection. These findings will provide a reference for formulating development policies about natural rubber industry in China. 展开更多
关键词 NATURAL RUBBER HEVEA brasiliensis PATENT ANALYSIS
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Molecular Cloning and Sequence Analysis of an Aquaporin Gene Related to Tapping Panel Dryness in Rubber Tree( Hevea brasiliensis Muell. Arg. )
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作者 Zhi ZOU Yeyong MO +2 位作者 Dahua WANG Feng AN Lifu YANG 《Agricultural Biotechnology》 CAS 2014年第2期37-39,43,共4页
As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree (Hevea brasiliensis ), "Tapping panel dryness (TPD)" causes great yield losses, thereby becoming the most imp... As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree (Hevea brasiliensis ), "Tapping panel dryness (TPD)" causes great yield losses, thereby becoming the most important factor limiting rubber production. On the basis of an EST down-regulated in TPD-affected rubber trees, a 903 bp cDNA denoted HbPIP2;2 was isolated from the bark tissue with a combination of in silico cloning and RT-PCR. The cDNA contains an 867 hp ORF, 13 bp 5'UTR and 23 bp 3' UTR. Sequence analysis indicated that HbPIP2;2 encodes 288 amino acids with a theoretical molecular weight (Mw) of 30. 71 kDa and isolectric point (Pi) of 8.20. Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the plasma membrane and harbor one conserved MIP domain that can be grouped into plasma membrane intrinsic proteins (PIPs) of aquaporin (AQP) family. Homolo- gy search revealed that the protein shares a similarity of more than 90% with the homologues in Ricinus communis, Popttlus trichocarpa, Juglans regia and Theobro- ma cacao, suooorting a hie.hly conserved evolution. This study provided basis for further uncovering the regulatory role of AOPs in TPD occurrence. 展开更多
关键词 Hevea brasiliensis Tapping panel dryness AQUAPORIN Plasma membrane intrinsic protein
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