Comparison between Four Types of Buffers for DNA Agarose Gel Electrophoresis

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.

Wedge-shaped HfO_(2) buffer layer-induced field-free spin-orbit torque switching of HfO_(2)/Pt/Co structure

Field-free spin-orbit torque(SOT)switching of perpendicular magnetization is essential for future spintronic devices.This study demonstrates the field-free switching of perpendicular magnetization in an HfO_(2)/Pt/Co/TaO_(x) structure,which is facilitated by a wedge-shaped HfO_(2)buffer layer.The field-free switching ratio varies with HfO_(2)thickness,reaching optimal performance at 25 nm.This phenomenon is attributed to the lateral anisotropy gradient of the Co layer,which is induced by the wedge-shaped HfO_(2)buffer layer.The thickness gradient of HfO_(2)along the wedge creates a corresponding lateral anisotropy gradient in the Co layer,correlating with the switching ratio.These findings indicate that field-free SOT switching can be achieved through designing buffer layer,offering a novel approach to innovating spin-orbit device.

Impact response and energy absorption of metallic buffer with entangled wire mesh damper

An innovative metallic buffer consisting of series-connected hat-shaped entangled wire mesh damper(EWMD)and parallel springs are proposed in this work to enhance the reliability of engineering equipment.The impact response and the energy dissipation mechanism of hat-shaped EWMD under different quasi-static compression deformations(2-7 mm)and impact heights(100-200 mm)are investigated using experimental and numerical methods.The results demonstrate distinct stages in the quasi-static mechanical characteristics of hat-shaped EWMD,including stiffness softening,negative stiffness,and stiffness hardening.The loss factor gradually increases with increasing compression deformation before entering the stiffness hardening stage.Under impact loads,the hat-shaped EWMD exhibits optimal impact energy absorption when it enters the negative stiffness stage(150 mm),resulting in the best impact isolation effect of metallic buffer.However,the impact energy absorption significantly decreases when hat-shaped EWMD enters the stiffness hardening stage.Interestingly,quasi-static compression analysis after experiencing different impact loads reveals the disappearance of the negative stiffness phenomenon.Moreover,with increasing impact loads,the stiffness hardening point progressively shifts to an earlier stage.

Non-destructive buffer enabling near-infrared-transparent inverted inorganic perovskite solar cells toward 1400 h light-soaking stable perovskite/Cu(In,Ga)Se_(2) tandem solar cells

Near-infrared(NIR)transparent inverted all-inorganic perovskite solar cells(PSCs)are excellent top cell candidates in tandem applications.An essential challenge is the replacement of metal contacts with transparent conductive oxide(TCO)electrodes,which requires the introduction of a buffer layer to prevent sputtering damage.In this study,we show that the conventional buffers(i.e.,small organic molecules and atomic layer deposited metal oxides)used for organic-inorganic hybrid perovskites are not applicable to all-inorganic perovskites,due to non-uniform coverage of the vulnerable layers underneath,deterioration upon ion bombardment and moisture induced perovskite phase transition,A thin film of metal oxide nanoparticles by the spin-coating method serves as a non-destructive buffer layer for inorganic PSCs.All-inorganic inverted near-infrared-transparent PSCs deliver a PCE of 17.46%and an average transmittance of 73.7%between 780 and 1200 nm.In combination with an 18.56%Cu(In,Ga)Se_(2) bottom cell,we further demonstrate the first all-inorganic perovskite/CIGS 4-T tandem solar cell with a PCE of 24.75%,which exhibits excellent illumination stability by maintaining 86.7%of its initial efficiency after 1400 h.The non-destructive buffer lays the foundation for efficient and stable NIR-transparent inverted inorganic perovskite solar cells and perovskite-based tandems.

Fast UAV path planning in urban environments based on three-step experience buffer sampling DDPG

The path planning of Unmanned Aerial Vehicle(UAV)is a critical issue in emergency communication and rescue operations,especially in adversarial urban environments.Due to the continuity of the flying space,complex building obstacles,and the aircraft's high dynamics,traditional algorithms cannot find the optimal collision-free flying path between the UAV station and the destination.Accordingly,in this paper,we study the fast UAV path planning problem in a 3D urban environment from a source point to a target point and propose a Three-Step Experience Buffer Deep Deterministic Policy Gradient(TSEB-DDPG)algorithm.We first build the 3D model of a complex urban environment with buildings and project the 3D building surface into many 2D geometric shapes.After transformation,we propose the Hierarchical Learning Particle Swarm Optimization(HL-PSO)to obtain the empirical path.Then,to ensure the accuracy of the obtained paths,the empirical path,the collision information and fast transition information are stored in the three experience buffers of the TSEB-DDPG algorithm as dynamic guidance information.The sampling ratio of each buffer is dynamically adapted to the training stages.Moreover,we designed a reward mechanism to improve the convergence speed of the DDPG algorithm for UAV path planning.The proposed TSEB-DDPG algorithm has also been compared to three widely used competitors experimentally,and the results show that the TSEB-DDPG algorithm can archive the fastest convergence speed and the highest accuracy.We also conduct experiments in real scenarios and compare the real path planning obtained by the HL-PSO algorithm,DDPG algorithm,and TSEB-DDPG algorithm.The results show that the TSEBDDPG algorithm can archive almost the best in terms of accuracy,the average time of actual path planning,and the success rate.

Irrigation and Thermal Buffering Using Mathematical Modeling

Two methods of irrigation,drip,and sprinkler were studied to determine the response of the Javits green roof to irrigation.The control study was dry unirrigated plots.Drip irrigation consisted of irrigation tubes running through the green roof that would water the soil throughout and sprinkler irrigation used a sprinkler system to irrigate the green roof from above.In all cases,the irrigated roofs had increased the soil moisture,reduced temperatures of both the upper and lower surfaces,reduced growing medium temperatures and reduced air temperatures above the green roof relative to the unirrigated roof.The buffered temperature fluctuations were also studied via air conditioner energy consumption.There was a 28%reduction in air conditioner energy consumption and a 33%reduction in overall energy consumption between dry and irrigated plots.Values of thermal resistance or S were determined for accuracy and for this study,there was little change which is ideal.A series of infra-red and thermal probe measurements were used to determine temperatures in the air and sedum.It was determined that the sprinkler irrigation did a better job than the drip irrigation in keeping cooler temperatures within the green roof.A Mann-Whitney U test was performed to verify the variation in moisture temperatures buffering energy consumption.By getting a p-value<0.05,it indicates that the model is accurate for prediction and medium temperatures were statistically different.

Numerical Study and Optimization of CZTS-Based Thin-Film Solar Cell Structure with Different Novel Buffer-Layer Materials Using SCAPS-1D Software

This study explored the performances of CZTS-based thin-film solar cell with three novel buffer layer materials ZnS, CdS, and CdZnS, as well as with variation in thickness of buffer and absorber-layer, doping concentrations of absorber-layer material and operating temperature. Our aims focused to identify the most optimal thin-film solar cell structure that offers high efficiency and lower toxicity which are desirable for sustainable and eco-friendly energy sources globally. SCAPS-1D, widely used software for modeling and simulating solar cells, has been used and solar cell fundamental performance parameters such as open-circuited voltage (), short-circuited current density (), fill-factor() and efficiency() have been optimized in this study. Based on our simulation results, it was found that CZTS solar cell with Cd0.4Zn0.6S as buffer-layer offers the most optimal combination of high efficiency and lower toxicity in comparison to other structure investigated in our study. Although the efficiency of Cd0.4Zn0.6S, ZnS and CdS are comparable, Cd0.4Zn0.6S is preferable to use as buffer-layer for its non-toxic property. In addition, evaluation of performance as a function of buffer-layer thickness for Cd0.4Zn0.6S, ZnS and CdS showed that optimum buffer-layer thickness for Cd0.4Zn0.6S was in the range from 50 to 150nm while ZnS offered only 50 – 75 nm. Furthermore, the temperature dependence performance parameters evaluation revealed that it is better to operate solar cell at temperature 290K for stable operation with optimum performances. This study would provide valuable insights into design and optimization of nanotechnology-based solar energy technology for minimizing global energy crisis and developing eco-friendly energy sources sustainable and simultaneously.

Temperature influences of the recoil characteristics for aluminum honeycomb buffer in the tether-net launcher

Fluctuations in outer space's temperature would affect the spacecraft's regular operation.This paper aims to study the temperature influences of the aluminum honeycomb buffer in the tether-net launcher.Firstly,a buffer structure was designed to attenuate the pyroshock generated by the pyrotechnic device.Secondly,the mechanical properties of aluminum honeycomb at different temperatures were obtained through quasi-static compression experiments.Then,the internal ballistic responses of the launcher were gained by the closed bomb tests and the equivalent classical interior ballistic model.Finally,the recoil performance of the launcher with aluminum honeycomb buffer at different temperatures was studied.It is revealed that the aluminum honeycomb crushing force gradually decreases with the temperature increases.The peak pressure,burning rate coefficient and velocity increase while the peak time decreases with the temperature increase for the interior ballistics.For the launcher recoil responses,the average launch recoil decreases if the aluminum honeycomb doesn't enter the dense stage.The impact acceleration,projectile velocity and displacement increase as the temperature increase.The paper spotlights the temperature's influence on the recoil characteristics of the aluminum honeycomb buffer,which provides a new idea for buffering technology of pyrotechnic devices in a complex space environment.

Influence of the lattice parameter of the AlN buffer layer on the stress state of GaN film grown on(111)Si

GaN films grown on(111)Si substrate with different lattice parameters of the AlN buffer layer by metal–organic chemical vapor deposition are studied.The stress states obtained by different test methods are compared and it is found that the lattice parameter of the AlN buffer layer may have a significant effect on the stress state in the initial stage of subsequent GaN film growth.A larger compressive stress is beneficial to improved surface morphology and crystal quality of GaN film.The results of further orthogonal experiments show that an important factor affecting the lattice parameter is the growth rate of the AlN buffer layer.This work may be helpful for realizing simple GaN-on-Si structures and thus reducing the costs of growth processes.

Micro-mechanism study of the effect of Cd-free buffer layers ZnxO(x = Mg/Sn) on the performance of flexible Cu2ZnSn(S, Se)4 solar cell

The traditional CdS buffer layers in flexible CZTSSe solar cells lead to light absorption losses and environmental pollution problems. Therefore, the study of Cd-free buffer layer is very important for the realization of environmentally friendly and efficient CZTSSe solar cells. The Zn1-xMgxO(ZnMgO) and Zn1-xSnxO(ZnSnO) alternate buffer layers are studied in this study using the simulation package solar cell capacitance simulator(SCAPS-1D) numerical simulation model, and the theoretical analysis is further verified by the results of the experiments. We simulate the performance of CZTSSe/ZnXO(X = Mg/Sn) heterojunction devices with different Mg/(Zn+Mg) and Sn/(Zn+Sn) ratios and analyze the intrinsic mechanism of the effect of conduction band offsets(CBO) on the device performance. The simulation results show that the CZTSSe/ZnXO(X = Mg/Sn) devices achieve optimal performance with a small “spike” band or “flat” band at Mg and Sn doping concentrations of 0.1 and 0.2, respectively. To investigate the potential of Zn_(0.9)Mg_(0.1O) and Zn_(0.8)Sn_(0.2)O as alternative buffer layers, carrier concentrations and thicknesses are analyzed. The simulation demonstrates that the Zn0.9Mg0.1O device with low carrier concentration has a high resistivity, serious carrier recombination, and a greater impact on performance from thickness variation. Numerical simulations and experimental results show the potential of the ZnSnO buffer layer as an alternative to toxic CdS, and the ZnMgO layer has the limitation as a substitute buffer layer. This paper provides the theoretical basis and experimental proof for further searching for a suitable flexible CZTSSe Cd-free buffer layer.

To Blend or Not to Blend? Anxiety Buffering Effects of Essential Oil Aromas

Essential oils of pure lavender and lavender blends have been employed as potential anxiolytic aromas in aromatherapy, but a direct comparison of their effectiveness is lacking. The current study investigated the effects of aroma on induced anxiety in non-clinical adults, comparing pure lavender, a commercially available blend and a no aroma control. An experimental, quantitative, mixed factorial design with an opportunity sample of 60 participants was employed. Participants were randomly allocated to three equal groups, one tested in a room infused with lavender aroma, the second with the doTerra Peace® blend, and the third free from any aroma. Participants’ state anxiety scores were measured before and after a novel video-based anxiety induction procedure. Data analysis revealed that the anxiety induction was successful and that both aromas delivered small to medium-sized buffering effects compared to no aroma. The findings add to a small body of research in an area where the practice is global yet has limited scientific evaluation. Future studies utilising brain imaging and blood serum analysis to investigate the anxiolytic mechanism of aromas would be beneficial to further our understanding.

NPBMT: A Novel and Proficient Buffer Management Technique for Internet of Vehicle-Based DTNs

Delay Tolerant Networks(DTNs)have the major problem of message delay in the network due to a lack of endto-end connectivity between the nodes,especially when the nodes are mobile.The nodes in DTNs have limited buffer storage for storing delayed messages.This instantaneous sharing of data creates a low buffer/shortage problem.Consequently,buffer congestion would occur and there would be no more space available in the buffer for the upcoming messages.To address this problem a buffer management policy is proposed named“A Novel and Proficient Buffer Management Technique(NPBMT)for the Internet of Vehicle-Based DTNs”.NPBMT combines appropriate-size messages with the lowest Time-to-Live(TTL)and then drops a combination of the appropriate messages to accommodate the newly arrived messages.To evaluate the performance of the proposed technique comparison is done with Drop Oldest(DOL),Size Aware Drop(SAD),and Drop Larges(DLA).The proposed technique is implemented in the Opportunistic Network Environment(ONE)simulator.The shortest path mapbased movement model has been used as the movement path model for the nodes with the epidemic routing protocol.From the simulation results,a significant change has been observed in the delivery probability as the proposed policy delivered 380 messages,DOL delivered 186 messages,SAD delivered 190 messages,and DLA delivered only 95 messages.A significant decrease has been observed in the overhead ratio,as the SAD overhead ratio is 324.37,DLA overhead ratio is 266.74,and DOL and NPBMT overhead ratios are 141.89 and 52.85,respectively,which reveals a significant reduction of overhead ratio in NPBMT as compared to existing policies.The network latency average of DOL is 7785.5,DLA is 5898.42,and SAD is 5789.43 whereas the NPBMT latency average is 3909.4.This reveals that the proposed policy keeps the messages for a short time in the network,which reduces the overhead ratio.

Simulation Study of CuO-Based Solar Cell with Different Buffer Layers Using SCAPS-1D

In copper oxide (CuO) based solar cells, various buffer layers such as CdS, In2S3, WS2 and IGZO have been investigated by solar cell capacitance simulator (SCAPS) in this work. By varying absorber and buffer layer thickness, photovoltaic parameters (open circuit voltage, fill factor, short-circuit current density and efficiency) are determined. The highest efficiency achieved is 19.6% with WS2 buffer layer. The impact of temperature on all CuO-based solar cells is also investigated.

Load-to-store:store buffer暂态窗口时间泄露的利用

为了研究现代处理器微架构中的漏洞并制定对应防护,针对负责管理访存指令执行顺序的内存顺序缓冲(MOB)进行分析,发现前向加载会把存在依赖的store指令的数据直接旁路到load指令,推测加载会提前执行不存在依赖的load指令,在带来效率优化的同时,也可能导致执行出错与相应的阻塞。针对Intel Coffee Lake微架构上现有MOB优化机制,分析如何利用内存顺序缓冲的4种执行模式与对应执行时间,构造包括暂态攻击、隐蔽信道与还原密码算法私钥的多种攻击。利用MOB引发的时间差还原内存指令地址,该地址可泄露AES T表实现的索引值。在Intel i5-9400处理器上对OpenSSL 3.0.0的AES-128进行了密钥还原实验,实验结果显示,30000组样本能以63.6%概率还原出一个密钥字节,且由于内存顺序缓冲的特性,该利用隐蔽性优于传统cache时间泄露。

In-situ constructed SnO_(2) gradient buffer layer as a tight and robust interphase toward Li metal anodes in LATP solid state batteries

Li_(1.3)Al_(0.3)Ti_(1.7)(PO_(4))_(3)(LATP),of much interest owing to its high ionic conductivity,superior air stability,and low cost,has been regarded as one of the most promising solid-state electrolytes for next-generation solid-state lithium batteries(SSLBs).Unfortunately,the commercialization of SSLBs is still impeded by severe interfacial issues,such as high interfacial impedance and poor chemical stability.Herein,we proposed a simple and convenient in-situ approach to constructing a tight and robust interface between the Li anode and LATP electrolyte via a SnO_(2)gradient buffer layer.It is firmly attached to the surface of LATP pellets due to the volume expansion of SnO_(2)when in-situ reacting with Li metal,and thus effectively alleviates the physical contact loosening during cycling,as confirmed by the mitigated impedance rising.Meanwhile,the as-formed SnO_(2)/Sn/LixSn gradient buffer layer with low electronic conductivity successfully protects the LATP electrolyte surface from erosion by the Li metal anode.Additionally,the LixSn alloy formed at the Li surface can effectively regulate uniform lithium deposition and suppress Li dendrite growth.Therefore,this work paves a new way to simultaneously address the chemical instability and poor physical contact of LATP with Li metal in developing low-cost and highly stable SSLBs.

Assessing Forest Cover Distribution, Species Diversity, and Richness of the Buffer Zone for Strategic Forest Management Planning for Nimule National Park, South Sudan

Natural forest ecosystems play an essential role in the conservation of biodiversity of many plants and animals by providing them with habitat and suitable environments. Studies have shown that biodiversity-protected areas reduce the loss and degradation of natural habitats to various wild species of plants. This study examined the forest resources in five subzones by conducting tree/shrub species inventory to be able to develop an effective forest management plan for the Buffer Zone Forest Reserve for the sustainable conservation of flora and fauna of Nimule National Park. This is with the view to identify the main tree and shrub species;assess the composition, distribution, and abundance of various tree/shrub species;to determine the species diversity as well as the richness of these areas in terms of growth and performance where DBH and heights of trees/shrubs in the circular sample plots were measured. The results revealed the overall mean DBH and H of 13.83 cm and 6.61 m respectively. The highest number of trees was obtained in subzone B followed by D, while the least were found in subzone A. The overall mean tree/shrub density was 0.83 tons/ha. The mean total basal area and volume per hectare were 3252.74 m2/ha and 46,540.82 m3/ha respectively. The overall species abundance and distribution indicate Combretum spp, Cedrella spp., Grewia mollis, Acacia Sieberiana, Ziziphus abysinica, and Acacia seyal were the most dominant species, with over 12 species richness at the deposition side of the River Nile, 13 species at the western side of the Nile, the Administration site shows only 7 species, the lowland of Mt. Gordon show over 14 different species, whereas over 10 species were found on the top of Mt. Gordon. The overall mean diversity indices and evenness of H’, D, and E depicted 2.507, 0.871, and 0.840 respectively. These results yielded are relatively moderate. Therefore, conservation efforts are very necessary to improve and maintain the quality of vegetation cover.

Detection of Buffer Overflow Attacks with Memoization-based Rule Set

Different abnormalities are commonly encountered in computer network systems.These types of abnormalities can lead to critical data losses or unauthorized access in the systems.Buffer overflow anomaly is a prominent issue among these abnormalities,posing a serious threat to network security.The primary objective of this study is to identify the potential risks of buffer overflow that can be caused by functions frequently used in the PHP programming language and to provide solutions to minimize these risks.Static code analyzers are used to detect security vulnerabilities,among which SonarQube stands out with its extensive library,flexible customization options,and reliability in the industry.In this context,a customized rule set aimed at automatically detecting buffer overflows has been developed on the SonarQube platform.The memoization optimization technique used while creating the customized rule set enhances the speed and efficiency of the code analysis process.As a result,the code analysis process is not repeatedly run for code snippets that have been analyzed before,significantly reducing processing time and resource utilization.In this study,a memoization-based rule set was utilized to detect critical security vulnerabilities that could lead to buffer overflow in source codes written in the PHP programming language.Thus,the analysis process is not repeatedly run for code snippets that have been analyzed before,leading to a significant reduction in processing time and resource utilization.In a case study conducted to assess the effectiveness of this method,a significant decrease in the source code analysis time was observed.

Z-Buffer精度问题的分析与解决

分析了在视景仿真应用中,由于普通显卡所支持的Z-Buffer的位数不够,当两个多边形距离很近时,计算机无法区分对应相同屏幕坐标的两个象素点的Z-Buffer的值,从而引起的闪烁现象。并提出了两种解决思路。

Acid Buffering Capacity of Forest Litter from Some ImportantPlantation and Natural Forests in South China

The role of forest litter as an acid-base buffering system was assessed by litter from plantation and natural forests in South China. Samples were either extracted with acid solutions or titrated with acid or base solutions. Litter was found to be a strong acid-base buffering system. Two legume species, Acacia mangium Willd and A. auriculaiformis A. Cunn, had very high litter pH values of around 6, which was 2 pH units higher than that of the soil where they grew. Litter of all other plantation species had litter pH of around 4, similar to that of the soil. Both legume species have high potential to neutralize soil acidity and the litter layer could act to shield soil against acid rain. The current stand of two legume species was estimated to be able to raise rain acidity by 0.1 to 0.4 pH units. Inorganic ions did not fully explain the pH pattern of different litter extracts, but high sodium and low nitrate partly accounted for the high pH of the two legume species. Some natural forest species had extremely low pH. As a whole, the litter of the natural climax forest was the driving force of soil acidification. Although plant residues are strong acid-base buffering system and able to adjust acidity of environment, only a few species can be expected to make soil more acid or alkaline through this mechanism since most species have litter pH values similar to those of soil where they grow.

Organic Photovoltaic Cells with Improved Performance Using Bathophenanthroline as a Buffer Layer

The role of bathophenanthroline （Bphen） as a buffer layer inserted between fullerene （C60） and Ag cathode in organic photovoltaic （OPV） cell was discussed. By introducing Bphen as a buffer layer with thicknes from 0 to 2.5 nm, the power conversion efficiency of the OPV cell based on copper phthalocyanine （CuPc） and C60 was increased from 0.87% to 2.25% under AM 1.5 solar illumination at an intensity of 100 mW/cm^2, which was higher than that of bathocuproine used as a buffer layer. The photocurrent-voltage characteristics showed that Bphen effectively improves electron transport through C60 layer into Ag electrode and leads to balance charge carrier transport capability. The influence of Bphen thickness on OPV cells was also investigated. Furthermore, the absorption spectrum shows that an additional Bphen layer enhances the light harvest capability of CuPc/C60.