Tree shrew models:A chronic social defeat model of depression and a one-trial captive conditioning model of learning and memory

Recent genome studies indicate that tree shrew is in the order or a closest sister of primates,and thus may be one of the best animals to model human diseases.In this paper,we report on a social defeat model of depression in tree shrew（Tupaia belangeri chinensis）.Two male tree shrews were housed in a pair-cage consisting of two independent cages separated by a wire mesh partition with a door connecting the two cages.After one week adaptation,the connecting door was opened and a brief fighting occurs between the two male tree shrews and this social conflict session consisted of 1 h direct conflict（fighting） and 23 h indirect influence（e.g.smell,visual cues） per day for 21 days.The defeated tree shrew was considered the subordinate.Compared with na？ve animals,subordinate tree shrews at the final week of social conflict session showed alterations in body weight,locomotion,avoidance behavior and urinary cortisol levels.Remarkably,these alterations persisted for over two weeks.We also report on a novel captive conditioning model of learning and memory in tree shrew.An automatic trapping cage was placed in a small closed room with a freely-moving tree shrew.For the first four trials,the tree shrew was not trapped when it entered the cage and ate the bait apple,but it was trapped and kept in the cage for 1 h on the fifth trial.Latency was defined as the time between release of the tree shrew and when it entered the captive cage.Latencies during the five trials indicated adaptation.A test trial 24 h later was used to measure whether the one-trial trapping during the fifth trial could form captive memory.Tree shrews showed much longer trapping latencies in the test trial than the adaptation trials.The N-methyl-d-aspartate（NMDA） receptor antagonist MK-801（0.2 mg/kg,i.p.）,known to prevent the formation of memory,did not affect latencies in the adaptation trails,but did block captive memory as it led to much shorter trapping latencies compared to saline treatment in the test trial.These results demonstrate a chronic social defeat model of depression and a novel one-trial captive conditioning model for learning and memory in tree shrews,which are important for mechanism studies of depression,learning,memory,and preclinical evaluation for new antidepressants.

Adobe Captive 3软件在信息技术教学中的应用

随着教育信息化的发展,如何设计制作出合适的带有交互功能的课件或者演示类的教程成了教师备课普遍存在的问题。本文介绍了信息技术的好帮手——AdobeCaptive3软件,并以Flash为例介绍了该软件使用的前期准备、录制过程、后期编辑等。该软件使用简便、功能强大,是教师制作演示类课件的首选。

Diurnal activity rhythms and time budgets of captive Qinling golden takin(Budorcas taxicolor bedfordi) in the Qinling Mountains,Shaanxi,China

In July 2006, the diurnal activity rhythms of 13 heads of captive Qinling golden takin were observed in Shaanxi Provincial Centre for the Breeding and Conservation of Rare Wildlife （SPCBCRW） at daylight （from 9：00 am to 4：00 pm in July）. The behavioral ethnogram was identified through observation at the sampling site, with the behaviors of golden takin recorded at 5 min interval using instantaneous scannable sampling method. The results indicated that the resting of golden takin took an important part in the daylight activities. Meanwhile, drinking and urinating and defecating were 1.92 and 0.54 and 0.92 times per head per day. Ambient temperature had a strong effect on the diurnal activity rhythms of golden takin. The diurnal activity rhymes were affected by ages of the animals effectively, which was expressed through differentiation of the time budgets. Moreover, different individuals in the same population showed some non-synchronously activity rhythms.

Captive Breeding of the Four-eyed Turtle(Sacalia quadriocellata)

In 1998,a study on forty-five four-eyed turtles(Sacalia quadriocellata) was initiated to gather preliminary biological data of this species and to investigate the feasibility of its captive reproduction.In the following six years,no courtship behavior was found occurring in males and no oviposition in females.From 2004 to 2007,two successful techniques were applied to initiate reproductive behavior:1) injecting exogenous reproductive hormones; and 2) reducing the stress of living in captivity.As a result of the hormone treatments,courtship behavior and copulation were observed during September and October,2005.However,no courtship displays were seen from the CK males,which were not treated with hormones.Ovulation occurred between December and March,and the correlation was not significant between behavior of ovulation and food intake.Females laid only one clutch of eggs each year,with 2.47 eggs(n=34,range=1–4) at average,and 84 eggs were totally obtained,of which 13 were damaged,52 were infertile and 19 fertile.Of the fertile eggs,nine were hatched with mean incubation period of 105.9 days(n=9,range=89–122 days) at temperature ranging from 24 to 27℃.

A growing captive population erodes the wild Red-crowned Cranes(Grus japonensis) in China

Background: The Red-crowned Crane(Grus japonensis) is an endangered bird species and while the wild population continues to decrease in China, the captive population has dramatically increased over the last two decades. We hypothesized that some of the captive Red-crowned Cranes originated from the wild and that a growing captive population is eroding the wild population in China.Methods: We surveyed the size of the population and determined the average annual growth rate, reproductive success rate and mortality rate of captive Red-crowned Cranes in 2013 in China. We assessed this erosion effect through mathematical models, in which the size of the captive Red-crowned Crane population was determined from the annual growth rate, the reproductive success rate and the rate of mortality.Results: We found there were a total of 1520 captive Red-crowned Cranes in 2013 in China, with an average annual gro-wth rate of 7.46%, a reproductive success rate of 9.17% and a mortality rate of 3.6%. We found that approximately 1027 supplementary Red-crowned Cranes per year and a total of 244, over the 14 year period from 1999 to 2013, were needed to account for the growing captive population in China.Conclusion: We conclude that the 244 birds probably came from the wild by taking eggs and capturing juveniles or adults and hence accepted the hypothesis. Perhaps more surprisingly, our annual estimate of the number of supplementary Red-crowned Cranes in captive populations is very conservative, with the erosion effect substantially underestimated, because the total number of captive Red-crowned Cranes in 2013 was underestimated, with the annual reproductive success rate in zoos overestimated. The existence of an erosion effect provides a new perspective for the interpretation of why the Red-crowned Crane population in the wild continues to decrease. In our opinion, it is important to understand the consequences of this erosion effect on the management and conservation of this endangered bird species in China.

Agroforestry Initiatives for Capacity Building and Social Security through Captive Plantations on Degraded Lands of Farmers in State Karnataka, India

To meet the demand of raw material, i.e., wood, from the available sources always remained a big challenge for paper industries since the last two decades. For a sustainable supply of raw material in future, major plantation activities were undertaken by paper industries, like West Coast Paper Mills Limited （WCPM）, by constituting a society, i.e., Society for Afforestation, Research and Allied Works （SARA）, Dandeli in 2001. The main aim was to promote afforestation programme through major plantations activities on the degraded lands （under rainfed condition） and to generate employments and capacity building. The WCPM is a leading paper manufacturing industry of the country （under the flagship of Bangur group） located at the bank of Kali River in Dandeli of Uttar Kannada district in State Karnataka. To date, company through SARA has raised captive plantations on more than 50,000 acres of degraded land by farmers in different districts of Maharashtm, Karnataka, Andhra Pradesh and Tamilnadu States under Captive Plantation Project through Contract for Farming （CFF） since 2006 under a simple agreement period of only five years. After five years, society arranged to harvest the first rotation crop of the farmer of 2006, 2007, 2008, 2009 plantations and paid the amount of the crop directly to farmers. SARA has a mutual contract with WCPM for purchasing pulpwood. With this, farmers are getting full amount of their crop harvested after five years without any hurdle through SARA. SARA did all the expenditures, i.e., from land development, plantation and harvesting. Under its agroforestry plantation programme, superior planting material of various pulpwood species suited to particular area, i.e., Eucalyptus, Subabul, Casuarina and Acacia were planted on farmer＇s land. Farmers were also encouraged for intercropping, like maize, peanut （groundnut）, chilly, tomato and ginger with plantations. Apart from this, SARA is also providing free fodder to the livestock of farmers. Various types of fodder species such as Stylosanthes scabra, S. hamata are grown with plantation. SARA has also set up its own hi-tech clonal nursery developing around 5-6 millions of clonal ramets every year through clonal propagation technology. Till date, SARA has received 0.15 million ton （MT） of pulpwood from the plantations on farmer＇s land with a yield of 25-30 MT/acre. This type of activities will prove a boon for farmers as well as for industry, when raw material is going to be short in India. It will also conserve our natural resources, high conservative valued forests （HCVF） and will reduce the pressure on forest for wood. In addition, this will maintain the balance between environment and nature. To meet the requirement of pulpwood for industry, this type of plantation activities will open new ways for industry to secure raw material for future use. Also, this would be helpful to enhance productivity and generate employments for rural communities to improve their living standard and capacity building.

Occurrence of gastrointestinal(GI)parasites in captive Olive Baboon and Common Langur in Bangladesh

Non-human primates(NHPs)serve as necessary reservoir hosts of parasites that create diseases to human.A close interaction between human and NHP can make a pathway for transmission of zoonotic diseases.To prevent zoonotic infection of zoo keepers,park visitors as well as keeping the captive NHPs in healthy state,it is necessary to carry out regular parasitological examination and treatment.A total of 72 fecal samples of Olive Baboon(n=39)and Common Langur(n=33)irrespective of their age and sex were collected from two zoological gardens of Bangladesh.Eggs and oocysts of seven gastrointestinal(GI)parasites were observed and identified in samples of both host species.The prevalence of GI parasites recorded was 100%.In case of Olive Baboon,the protozoan prevalence was higher(53.83%)than that of helminths,but opposite scenario was seen in case of Common Langur.Besides,higher intensity of coccidian oocysts in both hosts was recorded in the study.

Biological Status of Captive Crane in Southern Districts of Northern Pakistan

A study was aimed to overlook biological status including egg hatching, diet and diseases of common, Grus grus L. and demoiselle, Anthropoidedes virgo L. cranes in captive form in the southern districts of the Northern Pakistan. Field survey, questionnaire and interview with communities were the major tools for the data collection. Total 165 and 85 camps were visited, respectively, in fall, 2008 and spring, 2009. These camps were established in Baran dam, Kurram, Kashu, Kethu and Dowa in Bannu; and Gambilla, Lunder and Chall rivers in Lakki. The numbers of 1,650 hunters have 6,600 demoiselle and 3,300 common captive cranes in Bannu and Lakki, respectively. From 920 breeding pairs, 900 eggs were obtained, from which only 640 were hatched. Among natural foods of the cranes, snails, grasshoppers and earthworms, the pebbles were the most favorite foods. The young ones of cranes were fed on maize bread, eggs of insects and other small animals like wasp＇s larvae and grasshoppers by hunters. They faced the problems of development of feathers, trapping in mud and parasitic attack during their development. The cranes suffered from many diseases; head tumor, influenza and stomach blockage were the most common in the adults and young ones. The hunters used traditional things, garlic, coriander and brown sugar with antibiotics for treatments of diseases. Migratory cranes were found to be declining viewed by hunters in southern districts of Northern Pakistan. Knowledge about egg hatching, foods and diseases of common and demoiselle captive cranes, might be assisted in their conservation.

一种适合BRAS的Captive Portal方案

随着我国“互联网+”战略的推进,大型宽带网络发展迅速。BRAS设备在宽带网络中得到广泛使用,并通过IPoE方案组网,Portal认证是常用的网络准入解决方案。如何提升Portal系统性能,业界进行了广泛的研究。针对BRAS设备组建的宽带网,文中提出一种使用终端识别+网址判断的二元过滤方案,可有效地进行Portal的前置过滤,从而大幅降低无关的Portal请求,提升Portal认证系统的性能。

Captives Abuse Scandal Not Accidental

After the abuse of POWs and prisoners by US army in Iraq was brought to the day light in May, the worldwide denunciation silenced what the US was bragging about its support for human rights. The inhumane and brutal means used by the US army to torture Iraqi POWs and prisoners have gone to an alarming proportion. Photographs published show American soldiers—men and women—smiling, laughing or giving thumbs-up signs alongside naked Iraqi prisoners, exposing the sadistic and brutal methods employed by American forces and providing more evi-

Freeing Captive Animals

Even though theymight not haveenough food intheir own stom-achs,Tibetan peasantswould feed their draughtcattle with the best food,asthey depended on them forplowing. Such good treat-ment lasted until the ani-mals died,after which,some peasants would burythem in their own fields,

Predictors of dominance rank and agonistic interactions in captive Livingstone's fruit bats

Male dominance hierarchies have been studied in many animals but rarely in bats(Chiroptera).The dominance rank of social animals may dictate access to resources and mates;therefore,it has important implications for an individual's fitness and is crucial for successful captive man-agement.Between January and December 2018,at both Bristoi Zoo Gardens(Bristol,UK)and Jersey Zoo(Jersey,British Isles),we observed 19 male Livingstone's fruit bats Pteropus livingstonii using focal follows for 345 h overall,noting the outcome of all agonistic interactions.We recorded instigators of interactions,along with winners and losers,and analyzed these data using the R-package"EloRating"to create Elo-rating temporal plots of dominance ranks.We used generalized linear mixed models and multiple linear regression to analyze interaction data and test hypotheses regarding predictors of dominance rank,frequency of agonistic interaction,and choice of interaction partner.Age was positively correlated with dominance rank up to around year 9,when an asymptote was attained.Highly ranked bats instigated the most agonistic interac-tions,and largely directed these interactions at bats with much lower rankings than themselves.Hierarchies were extremely stable throughout the data collection period at both sites.We conclude that Livingstone's fruit bats have a stable linear dominance hierarchy,with high-ranking,typ-ically older males instigating the most interactions with lowest ranking males to secure dominance rank.This study adds to the limited discourse on Pteropus social behaviors,indicating that some bat species may have social systems similar in complexity to some nonhuman primates.

滤过型固相萃取柱净化-超高效液相色谱-串联质谱法测定鸡蛋中15种全氟化合物的含量

提出了滤过型固相萃取柱净化-超高效液相色谱-串联质谱法同时测定鸡蛋中全氟丁酸、全氟戊酸、全氟己酸、全氟庚酸、全氟辛酸、全氟壬酸、全氟癸酸、全氟十一酸、全氟十二酸、全氟十三酸、全氟十四酸、全氟丁烷磺酸、全氟己烷磺酸、全氟庚烷磺酸、全氟辛烷磺酸等15种全氟化合物含量的方法。鸡蛋样品(2 g)中加入0.1 mL 20.0μg·L^(-1)同位素内标混合溶液,经10 mL 80%(体积分数)乙腈溶液振荡和超声提取后,离心;分取5 mL滤液,直接过滤过型Captive EMR-Lipid柱净化,收集流出液,氮吹至近干,加入500μL甲醇复溶,经涡旋、离心处理后测定。采用Agilent Eclipse Plus C_(18)RRHD色谱柱(100 mm×2.1 mm,1.8μm)分离,以不同体积比的2 mmol·L^(-1)乙酸铵溶液和甲醇的混合溶液梯度洗脱,在电喷雾离子源负离子扫描模式下,以多反应监测模式检测,同位素内标法定量。结果表明:15种全氟化合物标准曲线的线性范围均为0.125~20.0μg·L^(-1),测定下限(10S/N)为0.05~0.16μg·kg^(-1);在0.500,4.00,16.0μg·kg^(-1)加标浓度水平下,15种目标物的回收率为78.0%~111%,测定值的相对标准偏差(n=6)为0.87%~14%。

超高效液相色谱-四极杆串联离子阱质谱法同时检测动物源性食品中26种新型抗病毒药物

目的建立一种超高效液相色谱-四极杆串联离子阱质谱法(ultra performance liquid chromatography quadrupole tandem ion trap-mass spectrometry,UPLC-MS/MS)同时检测动物源性食品中多种抗病毒药物的方法。方法样品经三氯乙酸-乙腈(20:80,V:V)溶液提取,Captive EMR-Lipid净化柱净化,采用Waters BEH C18色谱柱分离,UPLC-MS/MS检测,多反应监测-信息关联扫描-增强子离子扫描(multiple response monitoring-information dependent acquisition-enhanced product ion,MRM-IDA-EPI)确证,外标法定量。结果26种抗病毒药物定量限为0.1~5.0μg/kg,相关系数均高于0.99,回收率范围为71%~97%,相对标准偏差为1.3%~10.7%。结论采用新型通过型净化技术及四极杆串联离子阱质谱法,可同时对26种抗病毒药物进行定性、定量检测;采用二级全扫描谱图与谱库检索比对,实现了化合物的确证分析。

Metagenomics reveals contrasting energy utilization efficiencies of captive and wild camels(Camelus ferus)

Captive conditions can affect the symbiotic microbiome of animals.In this study,we compared the structural and functional differences of the gastrointestinal microbiomes of wild Bactrian camels(Camelus ferus)between wild and captive populations,as well as their different host energy utilization performances through metagenomics.The results showed that wild-living camels harbored more microbial taxa related to the production of volatile fatty acids,fewer methanogens,and fewer genes encoding enzymes involved in methanogenesis,leading to higher energy utilization efficiency compared to that of captive-living camels.These findings suggest that the wild-living camel fecal microbiome demonstrates a series of adaptive characteristics that enable the host to adjust to a relatively barren field environment.Our study provides novel insights into the mechanisms of wildlife adaptations to habitats from the perspective of the microbiome.

Behavioural responses of ex-situ captive hippopotamus (Hippopotamus amphibius) in lactation season: Maternal investment and plasticity of infant self-independence

In order to promote hippopotamus manage-ment in the captive and ex-situ environment,especially the control of behavioural and physiological status during breeding and lactation seasons,we conducted a prelimin-ary study on behavioural responses of a pair of hippos including both mother and infant in Hangzhou Wildlife Park,China.The study of the captive hippos for about 1-month in the lactation season was carried out during August and September,2009.The behavioural patterns were identified by all occurrence sampling and instanta-neous scanning sampling methods with 5–10 min inter-vals.As a result,mother-offspring conflicts and interactions did occur throughout the whole study period.Early maternal investment showed a positive trend in activity rhythms(slope=0.0014,Z=0.3027,P<0.001)and a negative trend(slope=–0.0066,Z=0.8807,P<0.001)in territorial occupation of water,all of which supported our hypotheses that the mother hippo might exert less care for the infant and cut down on her own obligations in nursing.For infant self-independence,during the whole lactation season,the primary trends of activities and territorial occupation dynamics of the infant hippo were slightly different from before,judging from linear models(slope=–0.0017,Z=0.3309,P<0.001).However,the frequencies of activities were not stable,especially at around 12 days of age.The trends of territorial occupation(slope=–0.0071,Z=0.904,P<0.001)also showed negative dynamics in water body occupation by the time the infant hippo grew up.The general trend(slope=–0.005,Z=0.06,P<0.001)of suckling dynamics was demonstrably negative,with an upwardsfluctuation at period 3(10–15th day).This also illustrated that as the infant developed,the dependency on the mother was reduced at the end of the lactation season.In addition,a sharp decline between P3 and P4 also supported the mother-offspring conflict theory.In general,time budgets of hippos in active behaviour were(31.8�2.1)%for the mother and(32.1�2.6)%for the infant.Spatial distribu-tions in water within temporal limitations were(80.1�2.7)%for the mother and(81.8�2.7)%for the infant.Behavioural dynamics showed strong synchronous relations between maternal investment and infant inde-pendence.Our current short-term investigation proves to be a key in management and conservation of hippopotami during the lactation season.

Ship maneuvering prediction based on virtual captive model test and system dynamics approaches

The maneuvering simulation is carried out through the continuous captive model test and the system dynamics approach.The mathematical maneuvering group(MMG)model is implemented in the virtual captive model tests by using the computational fluid dynamics(CFD)techniques.The oblique towing test(OTT),the circular motion test(CMT),the rudder force test and the open water test are performed to obtain the hydrodynamic derivatives of the hull,the rudder and the propeller,and the results are validated by experimental data.By designing the tests,the number of cases is reduced to a low level,to allow us to evaluate the maneuverability with a low cost and in a short time.Using these obtained coefficients,the system-based maneuvering simulations are conducted to calculate the position and the attitude of the ship,with results in agreement with the free running test results.This procedure can also be used for other hull forms,with reduced workload and with convenience for maneuvering simulation tasks.

Establish a special conservation zone for the captive giant panda

The giant panda(Ailuropoda melanoleuca)is recognized worldwide as an icon for wildlife conservation.Since the introduction in 1992 of reliable methods for artificial insemination,the population of captive pandas has grown to approximately 350 individuals in 2013.However,ca ptive panda populations are harmed by environmental pollution,diseases trans-mitted from domestic animals,and other anthropologenic activities.Although the Chinese government has proposed the creation of a Giant Panda National Park,there are at least three reasons that suggest that it is premature to reintroduce captive pandas into this proposed national park.First,habitat fragmentation remains the greatest threat to survival of giant pandas;second,most captive pandas are hybrids of the Sichuan and Qinling subspecies and release of hybrids may lead to further introgression between the two subspecies;and third,captive pandas may be competitively inferior to wild pandas in the region,and may not survive fights for food or mates.As an alternative,we suggest establishing a special con-servation zone in the Shennongjia National Nature Reserve for wild training of captive pandas prior to their reintroduction into the National Park.

Underwater video cameras allow for detection of North American giant salamanders (Cryptobranchus alleganiensis alleganiensis) in both captive and wild streams

Captive propagation and translocation are becoming vital components of conservation and management strategies for Eastern hellbender(Cryptobranchus a.alleganiensis)populations.Zoos,aquaria,universities,and state and federal agencies are concomitantly collaborating on the protection,education,and maintenance of captive populations of this unusual,cryptic salamander.Conservation strategies include the use of artificial nesting structures,collection of eggs from the wild,and head-starting individuals in zoos or hatcheries.The effects of these strategies need to be monitored,however,traditional survey and monitoring methods for the species in the wild involves rock-lifting,which has the potential to both harm habitat and alter reproductive behavior.Therefore,there is a need to develop effective,non-invasive and non-destructive methods of monitoring both wild and captive populations of Eastern hellbenders.Herein,we compare two simple,affordable,underwater video and camera systems(borescope and Aqua-Vu cameras)in their ability to 1)facilitate detection of adults under potential cover items and 2)facilitate nest detection and monitoring in both wild and captive environments.Both cameras were successful in detecting individual hellbender presence,albeit with different resolutions and detection times.The borescope was better at accessing deep cavities given its large flexible attachment which allowed for greater flexibility of scanning crevices of adult shelters.However,search time increased and even low levels of suspended sediment reduced visibility with the borescope.The Aqua-Vu camera provided greater overall visibility and faster detection of individuals under both natural and artificial shelters.There was a significant difference in the amount of time required to detect hellbenders with each camera design when searching under natural rocks(borescope:median=67.8 s,Aqua-Vu:median=39.1 s;Kruskal Wallis Test H=15.62,p<0.001)and artificial shelters(borescope:median=30.9 s,Aqua-Vu:median=13 s;Kruskal Wallis Test H=25.23,p<0.0001).We detected 8 natural nests with actively guarding males and only one individual using a wild artificial shelter.We recommend hellbender researchers utilize a combination of underwater video cameras to suit their specific survey goals in both captive and field settings.Moreover,we recommend zoo staff incorporate these methods to not only monitor captive populations but also to potentially record breeding behavior in zoos and aquariums.