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不同电学参数和水流条件下电栅对鲤(Cyprinus carpio)的阻拦效果
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作者 石小涛 张政 +6 位作者 黄晓龙 解立辉 李卫东 王渊洋 赵英华 李志敏 白艳勤 《海洋与湖沼》 CAS CSCD 北大核心 2024年第3期546-554,共9页
脉冲直流电导阻鱼技术已被广泛应用于阻止入侵物种扩散、驱赶鱼类远离危险区域并将其引导至有利区域,如鱼道。但是脉冲直流电的有效性受多方面因素的影响,其主导因素目前尚未定论。以鲤为研究对象,在静水条件下采用4因素3水平正交实验... 脉冲直流电导阻鱼技术已被广泛应用于阻止入侵物种扩散、驱赶鱼类远离危险区域并将其引导至有利区域,如鱼道。但是脉冲直流电的有效性受多方面因素的影响,其主导因素目前尚未定论。以鲤为研究对象,在静水条件下采用4因素3水平正交实验方法分析了脉冲电压、脉冲宽度、脉冲频率和电极摆放方式的电栅拦鱼效果及其对鱼类受伤尾数的主次影响,然后选择最优组合开展不同流速下(0,0.3,0.5 m/s)的驱鱼实验,分析流速对拦鱼效果的影响。结果表明:在静水工况下,影响拦鱼电栅效率的主要因素为电极布置方式>脉冲宽度>脉冲频率>脉冲电压,电极布置为方式1(垂直水流间距80 cm)、脉冲电压140 V、脉冲频率10 Hz、脉冲宽度10 ms是拦鱼效果最佳的组合。电学参数对鲤的伤害无显著性,平均受伤尾数不超过实验鱼总数的15%。流水条件下,三种流速工况下拦鱼电栅的阻拦率无显著差异,但是随着流速的增高阻拦率呈现先降低后增加的趋势。该研究将为保护鱼类资源和防止入侵鱼类跨区域传播提供依据。 展开更多
关键词 拦鱼电栅 阻拦效果 脉冲直流电
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Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies
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作者 Piao Zhao Lin Feng +7 位作者 Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1638-1656,共19页
Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food pollution.The developmental toxicity of OTA a... Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food pollution.The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear.This study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.Methods A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur group.Each treatment consisted of 3 replicates(180 fish)for 60 d.Results Firstly,we cultured,purified,and identified myoblasts using the tissue block culture method.Through preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect.Secondly,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass carp.Then,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling pathway.Finally,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling pathway.Conclusions In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro.This study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity. 展开更多
关键词 CURCUMIN Grass carp Myotoxic Ochratoxin A Protein deposition
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Anti-infective immune functions of type Ⅳ interferon in grass carp(Ctenopharyngodon idella):A novel antibacterial and antiviral interferon in lower vertebrates
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作者 Yuchen Liu Wentao Zhu +3 位作者 Yanqi Zhang Jingjing Zhang Maolin Lv Jianguo Su 《Zoological Research》 SCIE CSCD 2024年第5期972-982,共11页
Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated ... Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections. 展开更多
关键词 Grass carp(Ctenopharyngodon idella) IFN-υ Bactericidal activity Antiviral activity Antimicrobial immunity
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Research Progress on Nutritional Requirements of Carp
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作者 Yina LU 《Asian Agricultural Research》 2024年第1期37-39,43,共4页
Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yiel... Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yield has been greatly increased mainly due to the exten-sive use of compound feed.In this study,the nutritional requirements of carp were summarized from the aspects of protein,amino acids,fat,carbohydrate,calcium and phosphorus,vitamins and taurine.This study provides a certain theoretical reference for scientific formula of carp feed. 展开更多
关键词 carp Nutritional requirements Foreground
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Morphological changes of silver and bighead carp in the Yangtze River over the past 50 years 被引量:2
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作者 于红霞 唐文乔 李思发 《Zoological Research》 CAS CSCD 北大核心 2010年第6期651-656,共6页
Multivariate analysis was adopted to analyze 30 morphometrical characteristics of 121 one-year-old juvenile silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) bred during the 1950s ... Multivariate analysis was adopted to analyze 30 morphometrical characteristics of 121 one-year-old juvenile silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) bred during the 1950s ("the former population") and 2008 ("the current population") and collected from the middle reach of the Yangtze River. The average discriminant accuracies of the former and current silver and bighead carp population were 94.2% and 98.0%, respectively. Discriminant analysis also revealed that significant differences in morphology occurred between the former and current populations of both carp in overall characteristics. One-way analysis of variance indicated that between former and current populations, silver carp showed highly significant differences (P0.01) in twelve of their characteristics and significant differences (P0.05) in eight of their characteristics, while bighead carp showed highly significant differences (P0.01) in eight of their characteristics and significant differences (P0.05) in eight of their characteristics. Six head morphology variables of the current silver and bighead carp were significantly or highly significantly larger than the former populations; fourteen characteristics of silver carp and ten characteristics of bighead carp of the current populations, mainly reflecting truck and tail morphology, were significantly or very significantly smaller than the former populations. Our results indicate that silver and bighead carp have developed a larger head and smaller truck and tail during the last 50 years. Due to such morphological changes, it seems apparent that the heads of these fish species need to be considered in regards to human diets, particularly in relation to economic and nutritious value. 展开更多
关键词 Silver carp Bighead carp 50 years breeding Morphological change Yangtze River
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一种求解多车型CARP问题的高效进化算法 被引量:7
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作者 朱征宇 杨永 +3 位作者 邓欣 谢志华 夏梦霜 李小花 《计算机工程与应用》 CSCD 北大核心 2008年第8期212-216,共5页
对传统遗传算法的染色体编码机制和种群结构进行了改进,并借鉴单亲遗传算法和Memetic Algorithm(MA)算法的优秀思想,设计了一种解决CARP(Capacitated ArcRouting Problem)问题的高效算法HEGA。新算法不但有效解决了使用现有算法无力解... 对传统遗传算法的染色体编码机制和种群结构进行了改进,并借鉴单亲遗传算法和Memetic Algorithm(MA)算法的优秀思想,设计了一种解决CARP(Capacitated ArcRouting Problem)问题的高效算法HEGA。新算法不但有效解决了使用现有算法无力解决的多车型CARP问题,并且应用于一般的单车型CARP问题在求解效率和求解精度上也比现有MA算法效果更好。结合洒水车路径优化问题,通过一组真实的数据集合对文中算法在该问题上的求解能力做出评测。 展开更多
关键词 多车型 carp HEGA 洒水车路线优化
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Comparative Microsatellite Analysis of Grass Carp Genomes of Two Gynogenetic Groups and the Xiangjiang River Group 被引量:10
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作者 郑康 林凯东 +1 位作者 刘正华 罗琛 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第4期321-330,共10页
The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group (Xiangjiang group), a one-generation artificially induced meio-gynogenetic grass carp group (meio-gynogenetic-1 group), and a... The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group (Xiangjiang group), a one-generation artificially induced meio-gynogenetic grass carp group (meio-gynogenetic-1 group), and a two-generation artificially induced meio-gynogenetic grass carp group (meio-gynogenetic-2 group), were comparatively analyzed with microsatellite markers. Genetic polymorphism had been observed in the Xiangjiang group and most of the examined loci had more than two alleles. But the degree of genetic diversity was not very high. Although all the examined genetic loci in the analyzed individuals were in homozygous state, the genotypes of different individuals of the group were not identical in the meio-gynogenetic-1 group. In the meio-gynogenetic-2 group, not only the examined genetic loci of each individual were homozygous but also the genotypes of all the analyzed individuals of the group were the same. These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations. It was found that the polymorphism existed not only at the allele level but also at the locus level; many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis. Therefore, both protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are very important aspects for grass carp breeding. 展开更多
关键词 grass carp GYNOGENESIS GENOME microsatellite locus
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Microsatellite DNA Marker Analysis of Genetic Diversity in Wild Common Carp (Cyprinus carpio L.) Populations 被引量:6
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作者 李大宇 康大海 +2 位作者 殷倩茜 孙效文 梁利群 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第11期984-993,共10页
Thirty microsatellite loci were used for analyzing six wild populations of common carp (Cyprinus carpio L.). Observed (Ho) and expected (He) heterozygosity values, polymorphic information content (PIC), and nu... Thirty microsatellite loci were used for analyzing six wild populations of common carp (Cyprinus carpio L.). Observed (Ho) and expected (He) heterozygosity values, polymorphic information content (PIC), and number of effective alleles (Ae) were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package (version 3.63). There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1) the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2) the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution. 展开更多
关键词 molecular marker MICROSATELLITE population variety common carp
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恩诺沙星对湘云鲫(Triploid crucian carp)组织转氨酶(GPT、GOT)活性的影响 被引量:7
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作者 朱仙珍 郑永华 +1 位作者 唐洪玉 严玲 《淡水渔业》 CSCD 北大核心 2011年第1期58-63,共6页
采用胸腔注射法,研究了恩诺沙星对湘云鲫(Triploid crucian carp)组织谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性的影响。结果显示,给药后5 d,各试验组GPT、GOT酶活性均升高,与对照组之间呈显著差异水平(P<0.05);给药后10 d,各浓度组GPT... 采用胸腔注射法,研究了恩诺沙星对湘云鲫(Triploid crucian carp)组织谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性的影响。结果显示,给药后5 d,各试验组GPT、GOT酶活性均升高,与对照组之间呈显著差异水平(P<0.05);给药后10 d,各浓度组GPT酶活性有所降低,而GOT酶活性则继续升高,鳃的GOT酶活性达到了最大值;给药后15、20 d,各浓度组GPT酶活性继续下降,而肝脏、肾脏GOT酶活性在给药后15 d时达到最大值,之后开始下降;给药后25 d,鳃的GPT酶活性除200 mg/kg组外都恢复至正常水平,而肝脏、肾脏各浓度组的GPT酶活性显著低于对照组(P<0.05),肝脏的GOT酶活性恢复到了正常水平,而鳃和肾脏的GOT酶活性略高于对照组水平。 展开更多
关键词 恩诺沙星 湘云鲫(Triploid crucian carp) 谷丙转氨酶(GPT) 谷草转氨酶(GOT)
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Comparative Analysis on Growth and Morphology in Yangtze River ♀,Zhujiang River ♂ and Their F_1 Hybrids of Juvenile Grass Carp 被引量:6
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作者 傅建军 王荣泉 +3 位作者 刘峰 宣云峰 朱树人 李家乐 《Agricultural Science & Technology》 CAS 2009年第5期119-122,共4页
[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River... [ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River stock ♂ ), ZR (Zhujiang River stock ♀ ×Zhujiang River stock♂ ) and hybrids F1 ( Yangtze River stock ♀ × Zhujiang River stock ♂ ) were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations ( P〉0. 05), while there was an extremely significantly difference in those of 170 day-old generations ( P 〈0.01 ). However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR ( P 〉 0.05), but significant difference between F1 and YR ( P 〈 0.05). F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length ( P 〉 0.05), while significant difference in head length/total length, body height/standard length and body width/standard length ( P 〈 0.05), which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics. 展开更多
关键词 Grass carp Hybrid Growth performance Morphological parameter
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黄杞总黄酮对慢性心力衰竭大鼠AT1/CARP信号通路及心肌自噬的影响 被引量:6
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作者 解娜 王建发 +2 位作者 杨秀丽 殷国田 赵国安 《中国老年学杂志》 CAS 北大核心 2022年第11期2752-2756,共5页
目的探讨黄杞总黄酮对慢性心力衰竭(心衰)大鼠血管紧张素Ⅱ1型受体(AT1)/心肌锚定重复蛋白(CARP)信号通路及心肌自噬的影响。方法SD大鼠随机分成5组:对照组、模型组、缬沙坦组(50 mg/kg)、黄杞总黄酮低、高剂量组(250、500 mg/kg),每组1... 目的探讨黄杞总黄酮对慢性心力衰竭(心衰)大鼠血管紧张素Ⅱ1型受体(AT1)/心肌锚定重复蛋白(CARP)信号通路及心肌自噬的影响。方法SD大鼠随机分成5组:对照组、模型组、缬沙坦组(50 mg/kg)、黄杞总黄酮低、高剂量组(250、500 mg/kg),每组16只。模型组、缬沙坦组、黄杞总黄酮低、高剂量组大鼠建立慢性心衰模型,建模成功后,缬沙坦组、黄杞总黄酮低、高剂量组给予相应药物灌胃,对照组及模型组给予等体积的生理盐水,持续4 w。试验结束后,超声检查测定心功能指标,细胞自噬检测试剂盒检测心肌自噬指数,苏木素-伊红(HE)染色观察心肌病理结构变化,实时荧光定量PCR(qRT-PCR)及Western印迹测定心肌组织AT1、CARP mRNA和蛋白水平。结果与对照组比较,模型组大鼠左心室舒张末期内径(LVIDd)、左心室收缩末期内径(LVIDs)、自噬指数、心肌组织AT1、CARP mRNA和蛋白水平明显升高,左心室短轴缩短率(FS)、射血分数(EF)明显降低(P<0.05)。与模型组比较,缬沙坦组、黄杞总黄酮低剂量组、黄杞总黄酮高剂量组LVIDd、LVIDs、自噬指数、心肌组织AT1、CARP mRNA和蛋白水平明显降低,FS、EF明显升高(P<0.05);且黄杞总黄酮高剂量组LVIDd、LVIDs、自噬指数、心肌组织AT1、CARP mRNA和蛋白水平明显低于黄杞总黄酮低剂量组,FS、EF明显高于黄杞总黄酮低剂量组(P<0.05)。与缬沙坦组比较,黄杞总黄酮低剂量组LVIDd、LVIDs、自噬指数、心肌组织AT1、CARP mRNA和蛋白水平明显升高,FS、EF明显降低(P<0.05)。对照组心肌结构正常;模型组心肌横纹紊乱,心肌细胞明显肿胀,出现空泡情况,肌纤维溶解明显,有明显炎症细胞浸润;缬沙坦组及黄杞总黄酮高剂量组心肌炎症细胞浸润减少,心肌纤维排列整齐;黄杞总黄酮低剂量组心肌仍有明显病理变化。结论黄杞总黄酮对慢性心衰大鼠心功能及心脏病理改变有明显保护作用,能明显降低心肌细胞自噬水平;其机制可能与黄杞总黄酮可抑制心肌AT1、CARP mRNA和蛋白水平,进而抑制AT1/CARP信号通路的激活有关。 展开更多
关键词 黄杞总黄酮 慢性心衰 AT1/carp信号通路 心肌自噬
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Isolation of Microsatellite DNA and Preliminary Genomic Analysis of Mud Carp (Cirrhina molitorella) 被引量:10
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作者 程飞 叶卫 叶富良 《Zoological Research》 CAS CSCD 北大核心 2007年第2期119-125,共7页
In order to determine the applicability of microsatellite primers developed from common carp ( Cyprinuscario ) for genomic analysis in mud carp ( Cirrhina molitorella ), 24 primer pairs from common carp were desig... In order to determine the applicability of microsatellite primers developed from common carp ( Cyprinuscario ) for genomic analysis in mud carp ( Cirrhina molitorella ), 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers (54%) successfully amplified specific products in the mud carp and 11 primers (48%) showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity (Ho), unbiased expected heterozygosity (He) and polymorphism information content (PIC) in the wild population were 0.61 ± 0.2, 0.8 ± 0.09 and 0.72 ± 0.1 respectively. Several Hardy-Weinberg departure value were significandy departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input. 展开更多
关键词 Mud carp (Cirrhina molitorella MICROSATELLITE SCREENING Genomic polymorphism
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Comparative Study on Muscle Fiber Characteristics and Muscle Nutritional Quality between Two Groups of Yellow River Carps 被引量:3
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作者 杨又兵 卞军平 +2 位作者 马秀英 王伟 曾珍珍 《Agricultural Science & Technology》 CAS 2017年第8期1432-1436,1483,共6页
The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps ... The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps as the research objects, comparative study on muscle fiber diameter and density, routine nutritional composition, calcium and phosphorus contents and amino acids composition was conducted between the two groups of carps. The results showed that the moisture content was significantly higher (P 〈0.05), the crude fat and crude protein contents were significantly lower (P〈0.05), the essential amino acids and total amino acids contents were lower (P〉0.05), the phosphorus content was higher (P 〉0.05), and the calcium content was lower (P〉0.05) in the wild group compared with those in the farming group. The analysis of muscle fiber characteristics showed that there were significant differences in the average muscle fiber diameter (P〈0.05) and muscle fiber density (P〈0.01) between the two groups of Henan Yellow River carps. 展开更多
关键词 Yellow River carp PROTEIN Amino acids NUTRIENTS Muscle fiber
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Study on the Toxicity Effect of Abamectin and Chlorpyrifos on Grass Carp(Ctenopharyngodon idllus) 被引量:1
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作者 李常健 骆鹰 +2 位作者 杨锦兀 陈龙 蒋琼凤 《Plant Diseases and Pests》 CAS 2011年第1期67-70,共4页
[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp ( Ctenopharyngdon idllus ) and their interrela- tions. [ Method] Taking healthy and active grass carp as the ob... [ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp ( Ctenopharyngdon idllus ) and their interrela- tions. [ Method] Taking healthy and active grass carp as the object, the acute toxicity test of single pesticide and the combined toxicity test of two pesticides on grass carp were carried out. [ Result] The LC50 of abamectin and chlorpyrifes and the mixture of two pesticides against grass carp at 24, 48, 72 and 96 h were as follows: abamectin: 0.54, 0.49, 0. 17 and 0.10 rag/L; chlorpyrifes : 0.29, 0.21, 0.12 and 0.05 rag/L; the mixture of two pesticides : 1.22, 1.08, 0.99 and 0. 86 mg/L. The safe concentration (SC) of ahamectin, chlorpyrifos and the mixture of two pesticides were 0.010, 0.005 and 0.086 rag/L, respectively. The tox- icity of the pesticides in sequence was ablorpyrifos 〉 ahamectin 〉 the mixture of two pesticides. [ Conclusion ] Different concentrations of abamectin, chlorpyrifos and the mixture of two pesticides had remarkable effect on the growth of grass carp, the higher the concentration was, the greater the toxicity effect was. Ahamectin at low toxicity intensity had certain relief function on the toxicity of ehlorpyrifos. 展开更多
关键词 ABAMECTIN CHLORPYRIFOS Grass carp Acute toxicity
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Rates and patterns of microsatellite mutations in common carp (Cyprinus carpio L.) 被引量:1
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作者 张研 鲁翠云 +5 位作者 曹顶臣 徐鹏 王书 李恒德 赵紫霞 孙效文 《Zoological Research》 CAS CSCD 北大核心 2010年第5期561-564,共4页
During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per gene... During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp. 展开更多
关键词 Mutation rate Mutation pattern Common carp
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Optimization of Antibacterial Conditions in Freshcut Grass Carp Belly and Comparison of Shelf Life 被引量:2
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作者 侯温甫 何丽 刘伟 《Agricultural Science & Technology》 CAS 2016年第2期409-413,共5页
With fresh-cut grass carp belly as the test material, the antibacterial con- ditions of a disinfection agent sodium hypochlorite were optimized. In addition, the shelf lives of various grass carp products were compare... With fresh-cut grass carp belly as the test material, the antibacterial con- ditions of a disinfection agent sodium hypochlorite were optimized. In addition, the shelf lives of various grass carp products were compared after disinfection, so as to provide certain basis for the processing of fresh-cut grass carp products. The re- sults showed that the optimal disinfection conditions of sodium hypochlorite were as follows: concentration 300 mg/L, soak time 5 min and solid-liquid ratio 1 g : 5 ml. Under the optimal disinfection conditions, the inhibition rates of total bacteria and Pseudomonas reached 83% and 81%, respectively. The shelf life of refrigerated fresh-cut grass carp belly disinfected by sodium hypochlorite and packed in bag filled with gases could be extended as long as 11 d, which was increased by 5 d (83%) compared with that in the control group. The disinfection by sodium hypochlorite could significantly reduce the initial bacterial colony number and improve the sensory quality of fresh-cut grass carp belly, as well as extend the shelf life of refrigerated and modified gas-packed grass carp belly. 展开更多
关键词 Fresh-cut grass carp Sodium hypochlorite BACTERIOSTASIS Shelf life
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The Therapeutic Effect of Onion on the Pathogenic Bacteria of Gill Rot Disease in Grass Carp 被引量:2
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作者 徐大勇 韦汉群 张文丽 《Agricultural Science & Technology》 CAS 2008年第1期117-120,共4页
[Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched [Method] The combination method of taking medicine through oral and spraying was used to cure sick grass carp for ... [Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched [Method] The combination method of taking medicine through oral and spraying was used to cure sick grass carp for 1 period of treatment in room under artificial conditions. [Result] Different concentrations of onion generated different cure rates. When the combination was adding 1.0% - 2.0% medicine into feed and spraying 2.0 g,/m3 - 5.0 g,/m3, the curative result was the best with cure rate was 70% -90% [ Conclusion] The onion was effective on curing bacterial rotted gill disease in grass carp and could be taken as curative medicine. 展开更多
关键词 ONION Bacterial rotted gill disease Oral method Spraying method Grass carp
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Study on the Morphological Differences of Four Carp Populations 被引量:1
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作者 李倩 凌去非 +2 位作者 王磊 王大鹏 佟广军 《Agricultural Science & Technology》 CAS 2011年第9期1270-1272,1282,共4页
[Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data(including countable and measurable characters) and framework data,the morpho... [Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data(including countable and measurable characters) and framework data,the morphological differences and phylogenesis of Yellow River carp population(YR),wild four-naris carp population(WF),farmed four-naris carp population(FF) and carp population from Suzhou section of Wusong River(WR) were studied by means of multivariate analysis.[Result] There was no significant difference(P〉0.05) in countable characters among four carp populations.Discriminant analysis showed the discriminating accuracy of YR,WF,FF and WR population were 87.5%,78.1%,73.5% and 96.8% respectively,and the synthetic discriminating accuracy of four populations was up to 83.7%.In addition,cluster analysis revealed that YR population and WF population clustered together,while FF population and WR population clustered together.Principal component analysis indicated that the morphological differences among four populations mainly came from the head,tail and vertical axis of fish.[Conclusion] The study could provide theoretical foundations for the purification and rejuvenation of rare four-naris carp. 展开更多
关键词 Four-naris carp MORPHOLOGY Multivariate analysis
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ADAMTs-1、CF6、CARP在冠心病合并慢性心力衰竭中的意义 被引量:3
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作者 郭晓亮 袁宇 段长恩 《天津医科大学学报》 2021年第4期329-333,共5页
目的:分析Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(ADAMTs-1)、线粒体耦联因子-6(CF6)、心锚重复蛋白(CARP)在冠心病合并慢性心力衰竭(CHF)中的意义。方法:2017年4月—2020年4月收治90例冠心病合并CHF患者作为研究组,按照美国纽... 目的:分析Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(ADAMTs-1)、线粒体耦联因子-6(CF6)、心锚重复蛋白(CARP)在冠心病合并慢性心力衰竭(CHF)中的意义。方法:2017年4月—2020年4月收治90例冠心病合并CHF患者作为研究组,按照美国纽约心脏病学会对心功能进行分级,另分为3个亚组:心功能Ⅱ级组:28例,心功能Ⅲ级组:32例,心功能Ⅳ级组:30例;同时纳入同期接受健康体检者,共52例,作为对照组。收集所有研究对象一般资料,记录ADAMTs-1、CF6、CARP、脑钠肽(BNP)、左室舒张末期内径(LEVDD)、左室射血分数(LVEF)水平。比较研究组与对照组ADAMTs-1、CF6、CARP、BNP、LEVDD、LVEF水平差异及ADAMTs-1、CF6、CARP、BNP、LEVDD、LVEF水平在心功能分级中的表达情况;采用Pearson相关分析分别观察冠心病合并CHF患者ADAMTs-1、CF6及CARP水平与心功能分级的关系。结果:研究组患者ADAMTs-1、CF6、CARP、BNP及LVEDD水平显著高于对照组,而LVEF水平显著低于对照组,差异具有统计学意义(t=15.218、27.004、28.394、35.227、28.164,均P<0.05)。血清ADAMTs-1、CF6、CARP、BNP、LVEDD水平:Ⅳ级组>Ⅲ级组>Ⅱ级组,LVEF:Ⅳ级组<Ⅲ级组<Ⅱ级组,差异均具有统计学意义(F=34.94、68.87、260.62、141.85、1258.53、17.97,均P<0.05)。冠心病合并CHF患者ADAMTs-1、CF6、CARP水平与BNP、LVEDD呈正相关(均P<0.05),与LVEF呈负相关(P<0.05)。结论:ADAMTs-1、CF6、CARP水平对冠心病合并CHF患者具有重要意义。 展开更多
关键词 冠心病 慢性心力衰竭 ADAMTS-1 CF6 carp
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Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var.Jian) compared with mirror carp (Cyprinus carpio L.) 被引量:3
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作者 顾颖 鲁翠云 +3 位作者 张晓峰 李超 俞菊华 孙效文 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第3期636-649,共14页
We report the genetic linkage map of Jian carp(C yprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 c M and c... We report the genetic linkage map of Jian carp(C yprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 c M and comprised 44 linkage groups,with an average marker distance of 6.58 c M. We identified eight quantitative trait loci(QTLs) for body weight(BW) in seven linkage groups,explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp( Cyprinus carpio L.),which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map,with 186(93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups,mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp,which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp. 展开更多
关键词 Jian carp mirror carp body weight linkage map QTL comparative mapping
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