Cotton diseases represent a major challenge to cotton growth.Cloning of a cotton pathogen response gene and promoter is of great importance to improve disease resistance.In this study,a
Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involv...Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.展开更多
Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is...Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.展开更多
Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 ...Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 gene-mediated interaction with virus effector molecule. In this study, we searched for the susceptible allele of the “R” gene in Cv. T9. Southern hybridization study confirmed presence of an allele in Cv. T9. However, transcripts of the CYR1 could not be detected either by RT-PCR or by Northern hybridization in Cv. T9 and also in other susceptible blackgram line. The allele was isolated, sequenced and referred as cyr1. In silico study revealed that cyr1 also encodes a CC-NBS-LRR protein like CYR1. However the CC domain of cyr1 is truncated by 128 amino acid residues indicating functional impairment with respect to the signal transduction after pathogen invasion. Comparative 3D structural modeling, hydrogen bonding and Van der Waals interaction studies revealed differences between CYR1 and cyr1. Lys519 and Thr490 present in the largest pockets of the CYR1 are the key interacting hotspots between CYR1 and MYMIV coat protein (CP). The weak Van der Waals interactions and intermolecular hydrogen bonding between cyr1 and CP confers less stability to the molecular recognition complex, unlike CYR1. Thus, the present investigation revealed Cv. T9 shows compatible interaction with MYMIV due to the truncation in the cyr1 sequence and consequent structural difference in the N-terminal of CC-domain.展开更多
文摘Cotton diseases represent a major challenge to cotton growth.Cloning of a cotton pathogen response gene and promoter is of great importance to improve disease resistance.In this study,a
文摘Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.
基金the National Key Research and Development Program of China(2022YFD1201300)the Key R&D Program of Shandong Province(ZR202211070163)+1 种基金the Provincial Natural Science Foundation of Shandong(ZR2021ZD30,ZR2021MC056)the Young Taishan Scholars Program of Shandong Province.
文摘Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.
文摘Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 gene-mediated interaction with virus effector molecule. In this study, we searched for the susceptible allele of the “R” gene in Cv. T9. Southern hybridization study confirmed presence of an allele in Cv. T9. However, transcripts of the CYR1 could not be detected either by RT-PCR or by Northern hybridization in Cv. T9 and also in other susceptible blackgram line. The allele was isolated, sequenced and referred as cyr1. In silico study revealed that cyr1 also encodes a CC-NBS-LRR protein like CYR1. However the CC domain of cyr1 is truncated by 128 amino acid residues indicating functional impairment with respect to the signal transduction after pathogen invasion. Comparative 3D structural modeling, hydrogen bonding and Van der Waals interaction studies revealed differences between CYR1 and cyr1. Lys519 and Thr490 present in the largest pockets of the CYR1 are the key interacting hotspots between CYR1 and MYMIV coat protein (CP). The weak Van der Waals interactions and intermolecular hydrogen bonding between cyr1 and CP confers less stability to the molecular recognition complex, unlike CYR1. Thus, the present investigation revealed Cv. T9 shows compatible interaction with MYMIV due to the truncation in the cyr1 sequence and consequent structural difference in the N-terminal of CC-domain.
