目的探究皮层肌动蛋白结合蛋白(Cortactin)对异丙肾上腺素(isoprenaline,ISO)诱导的病理性心肌肥大的调控作用及其机制。方法采用ISO刺激新生大鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)24 h,在细胞水平建立心肌肥大模型;C57BL/6...目的探究皮层肌动蛋白结合蛋白(Cortactin)对异丙肾上腺素(isoprenaline,ISO)诱导的病理性心肌肥大的调控作用及其机制。方法采用ISO刺激新生大鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)24 h,在细胞水平建立心肌肥大模型;C57BL/6小鼠皮下注射ISO 1周,在动物水平建立心肌肥大模型。采用RT-qPCR检测mRNA的变化;免疫印迹法检测相应蛋白含量的变化;免疫荧光法检测Cortactin的亚细胞定位及表达量的变化;采用腺病毒感染的方法过表达Cortactin,通过转染小干扰RNA敲低Cortactin。结果在细胞和动物水平上,成功建立ISO诱导的心肌肥大模型,均观察到ISO引起Cortactin和N型钙黏连蛋白(N-cadherin)水平降低;过表达Cortactin可逆转ISO导致的N-cadherin蛋白水平的降低及心肌细胞肥大反应;敲低Cortactin则显示相反的效应。结论Cortactin可能联合N-cadherin通过增强心肌细胞之间的连接,发挥抗心肌肥大的作用。展开更多
Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance a...Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance and fieldevolved practical dual resistance of H.zea to these two toxins have been widely reported.Whether the widespread Cry1Ac/Cy2Ab dual resistance of H.zea has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains unclear.Cadherin is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran species.To test whether cadherin may serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab,we cloned H.zea cadherin(HzCadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and lossof-function analyses.Heterologous expression of HzCadherin in H.zea midgut,H.zea fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing HzCadherin of H.zea midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not Cry2Ab.Likewise,suppressing HzCadherin with siRNA made H.zea larvae resistant to Cry1Ac.These results clearly demonstrate that HzCadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab.展开更多
目的研究浆液性卵巢癌中E-钙粘蛋白(E-cadherin,E-cad),β-连环蛋白(β-catenin,β-cat),N-钙粘蛋白(N-cadherin,N-cad)的表达,探讨其相关性及临床意义。方法采用免疫组织化学法检测60例卵巢浆液性癌中E-cadherin,β-catenin and N-cadh...目的研究浆液性卵巢癌中E-钙粘蛋白(E-cadherin,E-cad),β-连环蛋白(β-catenin,β-cat),N-钙粘蛋白(N-cadherin,N-cad)的表达,探讨其相关性及临床意义。方法采用免疫组织化学法检测60例卵巢浆液性癌中E-cadherin,β-catenin and N-cadherin的表达。结果 E-cadherin,β-catenin在卵巢浆液性癌、卵巢浆液性良性肿瘤和卵巢交界性肿瘤及正常卵巢组织间差异有统计学意义(P<0.05),而在卵巢良性肿瘤和卵巢交界性肿瘤间,差异不大,无统计学意义(P>0.05)。N-cadherin在卵巢浆液性癌与卵巢交界性肿瘤,卵巢浆液性良性瘤及正常卵巢组织间差异有统计学意义(P<0.05),而在正常卵巢组织、卵巢良性肿瘤和卵巢交界性肿瘤间,无差异,无统计学意义。结论 E-钙粘蛋白(E-cadherin),β-连环蛋白(β-catenin),N-钙粘蛋白(N-cadherin)在浆液性卵巢癌中均存在异常表达。提示三种因子可能造成肿瘤的发生、发展、侵袭及转移。三者同时检测,可能为早期诊断上皮性卵巢癌及针对上皮性卵巢癌的靶向治疗提供分子依据及新的思路。展开更多
文摘目的探究皮层肌动蛋白结合蛋白(Cortactin)对异丙肾上腺素(isoprenaline,ISO)诱导的病理性心肌肥大的调控作用及其机制。方法采用ISO刺激新生大鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)24 h,在细胞水平建立心肌肥大模型;C57BL/6小鼠皮下注射ISO 1周,在动物水平建立心肌肥大模型。采用RT-qPCR检测mRNA的变化;免疫印迹法检测相应蛋白含量的变化;免疫荧光法检测Cortactin的亚细胞定位及表达量的变化;采用腺病毒感染的方法过表达Cortactin,通过转染小干扰RNA敲低Cortactin。结果在细胞和动物水平上,成功建立ISO诱导的心肌肥大模型,均观察到ISO引起Cortactin和N型钙黏连蛋白(N-cadherin)水平降低;过表达Cortactin可逆转ISO导致的N-cadherin蛋白水平的降低及心肌细胞肥大反应;敲低Cortactin则显示相反的效应。结论Cortactin可能联合N-cadherin通过增强心肌细胞之间的连接,发挥抗心肌肥大的作用。
基金funded by the USDA National Institute of Food and Agriculture (Hatch Grant ARZT-1360890-H31-164 and multi-state grant ARZ-T1370680-R31-172 (NC246))the National Natural Science Foundation of China (NSFC)–Henan Joint Major Grant (U2004206)+2 种基金the State Key Laboratory of Cotton Biology Open Fund, Zhengzhou University, China (CB2020A06)the Henan Agriculture Research System, China (HARS22-09-G3)the earmarked fund for China Agriculture Research System (CARS-27)
文摘Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance and fieldevolved practical dual resistance of H.zea to these two toxins have been widely reported.Whether the widespread Cry1Ac/Cy2Ab dual resistance of H.zea has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains unclear.Cadherin is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran species.To test whether cadherin may serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab,we cloned H.zea cadherin(HzCadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and lossof-function analyses.Heterologous expression of HzCadherin in H.zea midgut,H.zea fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing HzCadherin of H.zea midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not Cry2Ab.Likewise,suppressing HzCadherin with siRNA made H.zea larvae resistant to Cry1Ac.These results clearly demonstrate that HzCadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab.
文摘目的研究浆液性卵巢癌中E-钙粘蛋白(E-cadherin,E-cad),β-连环蛋白(β-catenin,β-cat),N-钙粘蛋白(N-cadherin,N-cad)的表达,探讨其相关性及临床意义。方法采用免疫组织化学法检测60例卵巢浆液性癌中E-cadherin,β-catenin and N-cadherin的表达。结果 E-cadherin,β-catenin在卵巢浆液性癌、卵巢浆液性良性肿瘤和卵巢交界性肿瘤及正常卵巢组织间差异有统计学意义(P<0.05),而在卵巢良性肿瘤和卵巢交界性肿瘤间,差异不大,无统计学意义(P>0.05)。N-cadherin在卵巢浆液性癌与卵巢交界性肿瘤,卵巢浆液性良性瘤及正常卵巢组织间差异有统计学意义(P<0.05),而在正常卵巢组织、卵巢良性肿瘤和卵巢交界性肿瘤间,无差异,无统计学意义。结论 E-钙粘蛋白(E-cadherin),β-连环蛋白(β-catenin),N-钙粘蛋白(N-cadherin)在浆液性卵巢癌中均存在异常表达。提示三种因子可能造成肿瘤的发生、发展、侵袭及转移。三者同时检测,可能为早期诊断上皮性卵巢癌及针对上皮性卵巢癌的靶向治疗提供分子依据及新的思路。
