Phytochemicals have been of great interest as a source of natural antioxidants used for health promotion, food preservation, food flavoring and cosmetics. In this research, alcoholic extract from Caralluma arabica and...Phytochemicals have been of great interest as a source of natural antioxidants used for health promotion, food preservation, food flavoring and cosmetics. In this research, alcoholic extract from Caralluma arabica and different solvent fractions were evaluated for their antioxidant, anti-inflammatory and anti-cancer properties as well as their polyphenolic compositions. The total antioxidant property was estimated by the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (33.1 mg gallic acid/g) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 143.64, 381.3, 112.6 μmol/g in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Caralluma arabica have strong antioxidant activity that was correlated with their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via Lipoxygenase inhibitory activity (LOX), the IC50 values ranged from 11.2 - 30.77 μg/mL. Moreover, Ethyl acetate fraction showed the strongest cytotoxic effect (IC50 = 87.55 μg/mL) against MCF-7 breast cancer cell line. These findings suggest that Caralluma arabica may be considered as an interesting source of antioxidants for nutraceutical industries.展开更多
We investigated the effects of a standardized extract of Caralluma fimbriata Wall (CFE) on learning and memory in mice using various behavioural models. Unusually, CFE exerts both nootropic and anxiolytic effects.
A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata ...A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata extract (CFE) to modify pre-adipocyte cell division and thus the development of hyper-plastic obesity. Mouse 3T3-L1 pre-adipocyte cell line samples were treated with different concentrations of an extract of CFE standardized against its pregnane glycoside content. Plain medium formed the negative control and hydroxyurea was the positive control. The cells were counted at 12-hour intervals, and their viability tested using the MTT assay. The treated cells were subjected to direct and indirect immunofluorescent assays for cyclin D1. CFE inhibited 3T3-L1 cell growth in a dose and duration-dependent manner, with results comparable to those produced by hydroxyurea. The viability of CFE-treated cells was reduced. Direct and indirect immunofluorescent assays demonstrated that CFE inhibits import of cyclin D1into the nucleus. CFE appears to inhibit pre-adipocyte cell division by interfering with a mechanism preceding the import of cyclin D1-CDk4/6 complex into the nucleus during the early G1 phase of the cell cycle, suggesting that CFE has the potential to inhibit hyperplastic obesity.展开更多
Six indigenous folk medicinal plants growing wild in the area of Tabuk, Saudi Arabia which were selected for the study of their phytochemistry as well as their biological activities as antitumor and antifolate agents....Six indigenous folk medicinal plants growing wild in the area of Tabuk, Saudi Arabia which were selected for the study of their phytochemistry as well as their biological activities as antitumor and antifolate agents. Antitumor activities of methanol extracts of the six plants were measured in vitro using three human tumor cell lines （breast, lung and CNS cancers） while antifolate activities were assessed using commercial dihyrofolate reductase obtained from Sigma Co. Among the six plant extracts tested, the most remarkable were those of Caralluma sinaica and Fagonia tenuifolia. Caralluma extract showed strong antitumor activity （low GIso） against the three human tumor cell lines. Fagonia extract, on the other hand, was quite inhibitory to the growth of CNS cancer and breast cancer cell lines but much less so against lung cancer cells. Extracts of both Sonchus oleraceus and Caralluma sinaica were strongly inhibitory to DHFR. These results suggest that the mechanism of anticancer activity of Caralluma plant is through DHFR inhibition but that of Fagonia may follow a different path.展开更多
文摘Phytochemicals have been of great interest as a source of natural antioxidants used for health promotion, food preservation, food flavoring and cosmetics. In this research, alcoholic extract from Caralluma arabica and different solvent fractions were evaluated for their antioxidant, anti-inflammatory and anti-cancer properties as well as their polyphenolic compositions. The total antioxidant property was estimated by the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (33.1 mg gallic acid/g) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 143.64, 381.3, 112.6 μmol/g in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Caralluma arabica have strong antioxidant activity that was correlated with their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via Lipoxygenase inhibitory activity (LOX), the IC50 values ranged from 11.2 - 30.77 μg/mL. Moreover, Ethyl acetate fraction showed the strongest cytotoxic effect (IC50 = 87.55 μg/mL) against MCF-7 breast cancer cell line. These findings suggest that Caralluma arabica may be considered as an interesting source of antioxidants for nutraceutical industries.
文摘We investigated the effects of a standardized extract of Caralluma fimbriata Wall (CFE) on learning and memory in mice using various behavioural models. Unusually, CFE exerts both nootropic and anxiolytic effects.
文摘A standardized extract of the plant Caralluma fimbriata (Slimaluma?) is widely used in the management of obesity but its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata extract (CFE) to modify pre-adipocyte cell division and thus the development of hyper-plastic obesity. Mouse 3T3-L1 pre-adipocyte cell line samples were treated with different concentrations of an extract of CFE standardized against its pregnane glycoside content. Plain medium formed the negative control and hydroxyurea was the positive control. The cells were counted at 12-hour intervals, and their viability tested using the MTT assay. The treated cells were subjected to direct and indirect immunofluorescent assays for cyclin D1. CFE inhibited 3T3-L1 cell growth in a dose and duration-dependent manner, with results comparable to those produced by hydroxyurea. The viability of CFE-treated cells was reduced. Direct and indirect immunofluorescent assays demonstrated that CFE inhibits import of cyclin D1into the nucleus. CFE appears to inhibit pre-adipocyte cell division by interfering with a mechanism preceding the import of cyclin D1-CDk4/6 complex into the nucleus during the early G1 phase of the cell cycle, suggesting that CFE has the potential to inhibit hyperplastic obesity.
文摘Six indigenous folk medicinal plants growing wild in the area of Tabuk, Saudi Arabia which were selected for the study of their phytochemistry as well as their biological activities as antitumor and antifolate agents. Antitumor activities of methanol extracts of the six plants were measured in vitro using three human tumor cell lines （breast, lung and CNS cancers） while antifolate activities were assessed using commercial dihyrofolate reductase obtained from Sigma Co. Among the six plant extracts tested, the most remarkable were those of Caralluma sinaica and Fagonia tenuifolia. Caralluma extract showed strong antitumor activity （low GIso） against the three human tumor cell lines. Fagonia extract, on the other hand, was quite inhibitory to the growth of CNS cancer and breast cancer cell lines but much less so against lung cancer cells. Extracts of both Sonchus oleraceus and Caralluma sinaica were strongly inhibitory to DHFR. These results suggest that the mechanism of anticancer activity of Caralluma plant is through DHFR inhibition but that of Fagonia may follow a different path.
