The Impact of Psychosocial Influences on Chronic Wound Healing

This comprehensive review explores the intricate dynamics between psychosocial factors and chronic wound healing processes, specifically focusing on prevalent conditions such as pressure ulcers, diabetic foot ulcers, and venous leg ulcers. By examining the roles of psychiatric conditions, including depression, anxiety, and post-traumatic stress disorder (PTSD), this paper illuminates how these factors intricately influence wound healing dynamics, including mechanisms of pain perception and inflammatory responses. Furthermore, we evaluate the effectiveness of integrated biopsychosocial interventions, which encompass a holistic approach to wound care, thereby enhancing healing outcomes for dermatology patients. Future studies should focus on investigating the specific psychosocial determinants that significantly influence wound healing, exploring novel therapeutic strategies, and implementing personalized interventions to meet the unique needs of each patient. Such endeavors hold promise in advancing the fields of psychodermatology and wound management, fostering a deeper understanding and application of psychosocial considerations in dermatological care.

Bilayer hydrogel dressing with lysozymeenhanced photothermal therapy for biofilm eradication and accelerated chronic wound repair

Biofilms are closely associated with the tough healing and dysfunctional inflammation of chronic wounds.Photothermal therapy(PTT)emerged as a suitable alternative which could destroy the structure of biofilms with local physical heat.However,the efficacy of PTT is limited because the excessive hyperthermia could damage surrounding tissues.Besides,the difficult reserve and delivery of photothermal agents makes PTT hard to eradicate biofilms as expectation.Herein,we present a GelMA-EGF/Gelatin-MPDA-LZM bilayer hydrogel dressing to perform lysozyme-enhanced PTT for biofilms eradication and a further acceleration to the repair of chronic wounds.Gelatin was used as inner layer hydrogel to reserve lysozyme(LZM)loaded mesoporous polydopamine(MPDA)(MPDA-LZM)nanoparticles,which could rapidly liquefy while temperature rising so as to achieve a bulk release of nanoparticles.MPDA-LZM nanoparticles serve as photothermal agents with antibacterial capability,could deeply penetrate and destroy biofilms.In addition,the outer layer hydrogel consisted of gelatin methacryloyl(GelMA)and epidermal growth factor(EGF)promoted wound healing and tissue regeneration.It displayed remarkable efficacy on alleviating infection and accelerating wound healing in vivo.Overall,the innovative therapeutic strategy we came up with has significant effect on biofilms eradication and shows promising application in promoting the repair of clinical chronic wounds.

Phospholipid-grafted PLLA electrospun micro/nanofibers immobilized with small extracellular vesicles from rat adipose mesenchymal stem cells promote wound healing in diabetic rats

Small extracellular vesicles(sEVs)derived from mesenchymal stem cells(MSCs)can deliver a variety of bioactive factors to create a favorable local microenvironment,thereby holding huge potential in chronic wound repair.However,free sEVs administrated intravenously or locally are usually cleared rapidly,resulting in an insufficient duration of the efficacy.Thus,strategies that enable optimized retention and release profiles of sEVs at wound sites are desirable.Herein,we fabricated novel functional phosphoethanolamine phospholipid-grafted poly-L-lactic acid micro/nanofibers(DSPE-PLLA)to carry and retain sEVs from rat adipose MSCs,enabling the slow local release of sEVs.Our results showed that sEVs@DSPE-PLLA promoted the proliferation,migration and gene expression(ColⅠ,ColⅢ,TGF-β,α-SMA,HIF-1α)of fibroblasts.It also promoted keratinocyte proliferation.In addition,sEVs@DSPE-PLLA helped polarize macrophages toward the M2 phenotype by increasing the expression of anti-inflammatory genes(Arginase 1,CD 206,IL-10)and inhibiting the expression of pro-inflammatory genes(IL-1β,TNF-α).Further in vivo study in diabetic rat models showed that sEVs@DSPE-PLLA improved the wound-healing process by alleviating the inflammatory responses,stimulating cell proliferation,collagen deposition and angiogenesis.These results highlight the potential of using DSPE-grafted scaffolds for extracellular vesicle immobilization and suggest sEVs@DSPE-PLLA micro/nanofibers as promising functional wound dressings for diabetic wounds.

A pro-healing short-chain antimicrobial peptide that inhibits sepsis

Chronic wounds experiencing infections with multidrug-resistant bacteria can be fatal,and in severe cases can lead to sepsis.Antimicrobial peptides are widely used in the field of wound care for their broad-spectrum antibacterial properties and good anti-drug resistance.We prepared bacterial cell membrane chromatography(BCMC)by extracting cell membranes of bacteria using SiO_(2)microspheres as stationary phase.A library of antimicrobial peptides was synthesized in solid phase and screened by BCMC to identify the antimicrobial peptide LKAHR(later named LS5),which is characterized by biosafety,broad-spectrum antibacterial activity,and drug resistance,and a gelatin-based antimicrobial hydrogel(LS5-gel)was prepared to be better applied to wounds.LS5-gel was found to have good in vivo bactericidal properties as well as the ability to promote wound healing in a wound healing model.In the sepsis model,LS5 was found to have a significant inhibitory effect on sepsis infection.It is important for the selection of next-generation antimicrobial drugs and the treatment of chronic wound healing.

Molecular docking-aided AIEgen design: concept, synthesis and applications

Aggregate-induced emission luminogens(AIEgens) have been widely used in biological imaging, chemical sensing, and disease treatments. The rational design and construction of AIEgens have received considerable research interests during the last few years. Herein, molecular docking-aided AIEgen design has been reasonably proposed and AIEgen TBQZY with excellent ~1O_(2) generation ability has been synthesized. The newly developed TBQZY could efficiently kill S. epidermidis and methicillinresistant S. epidermidis(MRSE) by tightly binding to bacteria and triggering the accumulation of ~1O_(2) in bacteria. TBQZY specifically regulated the immune system and polarized macrophages from M1 to M2 to accelerate the elimination of biofilm in vivo. In addition, healing acceleration was observed in chronic wounds treated with TBQZY, and side effects were negligible.Meanwhile, TBQZY had extraordinary potential for combating drug-resistant bacteria in the clinical setting. This research not only provided new concepts for the design of AIEgens, but also shed some lights on the discovery of drugs against drug-resistant bacteria.