【目的】研究葡萄白藜芦醇的诱导剂,为开发新的葡萄保鲜剂与农药提供参考。【方法】采用Fe Cl3水溶液对红地球葡萄叶片愈伤组织进行诱导,应用HPLC分析愈伤组织中白藜芦醇的含量变化,DAB染色法检测活性氧水平,使用RT-PCR方法分析茋合酶基...【目的】研究葡萄白藜芦醇的诱导剂,为开发新的葡萄保鲜剂与农药提供参考。【方法】采用Fe Cl3水溶液对红地球葡萄叶片愈伤组织进行诱导,应用HPLC分析愈伤组织中白藜芦醇的含量变化,DAB染色法检测活性氧水平,使用RT-PCR方法分析茋合酶基因(STS)的表达量变化。【结果】Fe Cl3溶液对葡萄愈伤组织茋类物质具有显著的诱导作用,诱导白藜芦醇的积累呈现显著的量时依赖性;1.6 m M Fe Cl3溶液处理18 h后愈伤组织中白藜芦醇含量可提高约8.5倍,鲜重达到210.8μg/g。Fe Cl3溶液处理组的活性氧水平均高于对照组;加抗氧化剂CAT和NAC可明显降低Fe Cl3对白藜芦醇诱导效果。【结论】Fe Cl3溶液对红地球葡萄叶愈伤组织白藜芦醇积累具有显著升高作用,其积累机制与Fe Cl3所导致的氧胁迫正相关。展开更多
Phase behaviors of different binary systems involving 1-dodecyl-3-methylimidazolium chlo- ride （[C12mim]Cl） and H20, [C12mim]Cl and different alcohols （1-butanol, 1-pentanol, 1- hexanol and 1-octanol） are investig...Phase behaviors of different binary systems involving 1-dodecyl-3-methylimidazolium chlo- ride （[C12mim]Cl） and H20, [C12mim]Cl and different alcohols （1-butanol, 1-pentanol, 1- hexanol and 1-octanol） are investigated at 25 ℃. Hexagonal liquid crystal phase （H1） is identified in [C12mim]Cl/H2O system, and lamellar liquid-crystalline （Lα） phase is found in [C12mim]Cl/alcohols systems by using polarized optical microscopy and small-angle X-ray scattering techniques. The formation of such phases is considered as a synergetic result of the solvatophobic force and the hydrogen-bonded network comprising an imidazoliuin ring, chloride ion and water （or alcohols）, which can be confirmed by Fourier transform infrared spectra. It is noticeable that in [C12mim]Cl/1-octanol system, the lattice spacings of lamellar phase increase with increasing C12mimCl concentration, which is opposite to the results of [C12mim]Cl/H2O system. This may result mainly from stronger static repulsion among hydrophilic headgroups of imidazolium salts arranged in the bilayers of lamellar structures. Further measurements by differential scanning calorimetry indicate that the lamellar phase is stable within a wide temperature range above room temperature. However, the lattice spacings decrease with the increase of temperature, which may. be due to the softening of the hydrocarbon chain of [C12mim]Cl molecules. In different alcohols systems, it is found that the lamellar lyotropic liquid crystal structure is easier to be formed when the carbon chain length becomes longer.展开更多
文摘【目的】研究葡萄白藜芦醇的诱导剂,为开发新的葡萄保鲜剂与农药提供参考。【方法】采用Fe Cl3水溶液对红地球葡萄叶片愈伤组织进行诱导,应用HPLC分析愈伤组织中白藜芦醇的含量变化,DAB染色法检测活性氧水平,使用RT-PCR方法分析茋合酶基因(STS)的表达量变化。【结果】Fe Cl3溶液对葡萄愈伤组织茋类物质具有显著的诱导作用,诱导白藜芦醇的积累呈现显著的量时依赖性;1.6 m M Fe Cl3溶液处理18 h后愈伤组织中白藜芦醇含量可提高约8.5倍,鲜重达到210.8μg/g。Fe Cl3溶液处理组的活性氧水平均高于对照组;加抗氧化剂CAT和NAC可明显降低Fe Cl3对白藜芦醇诱导效果。【结论】Fe Cl3溶液对红地球葡萄叶愈伤组织白藜芦醇积累具有显著升高作用,其积累机制与Fe Cl3所导致的氧胁迫正相关。
文摘Phase behaviors of different binary systems involving 1-dodecyl-3-methylimidazolium chlo- ride （[C12mim]Cl） and H20, [C12mim]Cl and different alcohols （1-butanol, 1-pentanol, 1- hexanol and 1-octanol） are investigated at 25 ℃. Hexagonal liquid crystal phase （H1） is identified in [C12mim]Cl/H2O system, and lamellar liquid-crystalline （Lα） phase is found in [C12mim]Cl/alcohols systems by using polarized optical microscopy and small-angle X-ray scattering techniques. The formation of such phases is considered as a synergetic result of the solvatophobic force and the hydrogen-bonded network comprising an imidazoliuin ring, chloride ion and water （or alcohols）, which can be confirmed by Fourier transform infrared spectra. It is noticeable that in [C12mim]Cl/1-octanol system, the lattice spacings of lamellar phase increase with increasing C12mimCl concentration, which is opposite to the results of [C12mim]Cl/H2O system. This may result mainly from stronger static repulsion among hydrophilic headgroups of imidazolium salts arranged in the bilayers of lamellar structures. Further measurements by differential scanning calorimetry indicate that the lamellar phase is stable within a wide temperature range above room temperature. However, the lattice spacings decrease with the increase of temperature, which may. be due to the softening of the hydrocarbon chain of [C12mim]Cl molecules. In different alcohols systems, it is found that the lamellar lyotropic liquid crystal structure is easier to be formed when the carbon chain length becomes longer.
