施氮量对裸燕麦源库生理特性和茎鞘NSC积累与转运的影响

为探究施氮量对燕麦源库生理特性和茎鞘间非结构性碳水化合物(NSC)积累和转运的影响,2021年和2022年选取穗粒数差异大的两个裸燕麦品种坝莜1号(穗粒数多)和定莜8号(穗粒数少)为试验材料,设置0、100和200 kg·hm^(-2)3个施氮水平,测定和分析了不同氮素供应条件下燕麦叶片光合指标、粒叶比、茎鞘NSC积累量(TMNSC)、NSC表观转运量(ATMNSC)及其对籽粒产量表观贡献率(ACNSC)的差异。结果表明,施氮对2个燕麦品种的籽粒产量具有增加效应,其中在施氮100 kg·hm^(-2)时产量最高。在100 kg·hm^(-2)施氮处理下,坝莜1号的旗叶面积、SPAD值、Pn和籽粒产量两年平均值较不施氮处理分别提高57.57%、80.70%、101.68%和40.15%,定莜8号分别提高43.70%、44.33%、69.49%和37.36%;坝莜1号的ATMNSC、ACNSC和粒叶比两年平均值较不施氮处理分别增加767.25 g、1.96倍和54.55%,定莜8号分别增加859.52 g、8.26倍和43.25%。综合以上结果,增施氮肥对两品种均有显著正向影响,坝莜1号表现出更优的源、库活性,从而获得更高的穗粒数,达到增产目的;定莜8号则表现出更优的源-库关系,增大库器官对源物质的“拉力”,促进NSC由茎鞘向籽粒的转运,弥补生育前期光合能力较弱导致的同化物质生产的不足,从而促进源库协调,提高籽粒产量。

稳定转染TDP-43导致NSC34细胞自噬异常

目的 探讨稳定转染TDP-43对NSC34细胞自噬的影响。方法 体外培养稳定转染空质粒的NSC34细胞系(E细胞)和转染TDP-43的NSC34细胞系(TDP-43细胞)。应用CCK-8试剂盒测定两组细胞活力,应用EdU试剂盒测定两组细胞增值能力,应用透射显微镜观察两组细胞线粒体形态,应用免疫荧光检测两组细胞自噬相关蛋白表达水平。结果 与E组细胞相比,TDP-43组细胞活力明显下降,增殖能力下降,差异有统计学意义(P<0.05);线粒体形态异常,自噬相关蛋白表达水平升高,差异有统计学意义(P <0.05)。结论 稳定转染TDP-43可导致NSC34细胞自噬异常,线粒体形态改变,降低细胞活力和增殖能力。

基于UHPLC-MS技术的NSCLC血浆生物学标志物的筛选及鉴定研究

目的研究UHPLC-MS技术的NSCLC血浆生物学标志物的筛选及鉴定。方法本研究纳入符合非小细胞肺癌临床诊断标准的60例NSCLC患者,随机分为两组,接受靶向治疗(TACE)的非小细胞肺癌患者为鉴别诊断组,未接受靶向治疗的NSCLC患者为正常对照组。结果在60例NSCLC患者中,通过LC-MS/MS技术共检测到552个生物标志物。在其中17个生物标志物中,有8个出现在已知的EGFR突变患者中,另7个出现在ALK融合患者中,6个出现在HER2突变患者中。此外,NSCLC患者血浆中含有丰富的非编码RNA(miRNA),包括58个miRNA和23个mRNA。结论本研究中,采用LC-MS/MS技术对NSCLC患者血浆进行分析,可快速、高效地获得血浆样品中的生物学标志物。这些标志物可以作为NSCLC诊断的潜在生物学标志物,并且可以用于预测肿瘤进展和不良预后。

Early Devonian Post-collisional Granitic Magmatism in the North Qilian Orogenic Belt,Western China:Insights into Lithospheric Delamination and Orogenic Collapse

Post-collisional magmatism contains important clues for understanding the reworking and growth of continental crust,as well as lithospheric delamination and orogenic collapse.Early Devonian magmatism has been identified in the North Qilian Orogenic Belt(NQOB).This paper reports an integrated study of petrology,whole-rock geochemistry,Sm-Nd isotope and zircon U-Pb dating,as well as Lu-Hf isotopic data,for two Early Devonian intrusive plutons.The Yongchang and Chijin granites yield zircon U-Pb ages of 394-407 Ma and 414 Ma,respectively.Both of them are characterized by weakly peraluminous to metaluminous without typical aluminium-rich minerals,LREE-enriched patterns with negative Eu anomalies and a negative correlation between P_(2)O_(5) and SiO_(2) contents,consistent with geochemical features of I-type granitoids.Zircons from the studied granites display negative to weak positive ε_(Hf)(t)values(−5.7 to 2.1),which agree well with those of negative ε_(Nd)(t)values(−6.4 to−2.9)for the whole-rock samples,indicating that they were derived from the partial melting of Mesoproterozoic crust.Furthermore,low Sr/Y ratios(1.13-21.28)and high zircon saturation temperatures(745℃ to 839℃,with the majority being>800℃)demonstrated a relatively shallow depth level below the garnet stability field and an additional heat source.Taken together,the Early Devonian granitic magmatism could have been produced by the partial melting of ancient crustal materials heated by mantle-derived magmas at high-temperature and low-pressure conditions during postcollisional extensional collapse.The data obtained in this study,when viewed in conjunction with previous studies,provides more information about the tectonic processes that followed the closure of the North Qilian Ocean.The tectonic transition from continental collision to post-collisional delamination could be constrained to~430 Ma,which is provided by the sudden decrease of Sr/Y and La/Yb ratios and an increase in zircon ε_(Hf)(t)values for granitoids.A two-stage tectonic evolution model from continental collision to post-collisional extensional collapse for the NQOB includes(a)continental collision and crustal thickening during ca.455-430 Ma,characterized by granulite-facies metamorphism and widespread low-Mg adakitic magmatism;(b)post-collisional delamination of thickened continental crust and extensional collapse of orogen during ca.430-390 Ma,provided by coeval high-Mg adakitic magmatism,A-type granites and I-type granitoids with low Sr-Y ratios.

Roof collapse mechanism of weak surrounding rock for deep-buried tunnels under high geostress conditions

High geostress,a typical attribute of tunnels located at significant depths,is crucial in causing stress-induced failure and influencing the stability of the tunnel crown.This study developed an analytical method for the failure mechanism that occurs in deep-buried tunnel roofs,taking into account the influence of geostress.The limit analysis theory was utilized for deriving analytical solutions about the geometry of the collapsing surface and the limit supporting pressure.The collapsing surface obtained by the analytical solution was validated by the findings of the physical model test,which shows a high level of agreement with the actual one.An extensive investigation was done to explore the effects of the lateral pressure coefficients,the tunnel buried depth,the geological conditions of the surrounding rock,the long-short axis ratio,and the size of the tunnel profile.The findings indicate that an increase in the lateral pressure coefficient from 0.5 to 1.5 results in a reduction in the height of the collapsing zone by 2.08 m and the width of the collapsing zone by 1.15 m,while simultaneously increases the limit supporting pressure by 18.9%.The proposed upper bound method accurately determines the limit supporting pressure and the geometry of the collapsing surface,which aligns well with the results acquired through numerical modelling and on-site monitoring in actual engineering applications.The proposed analytical method can serve as a reference for similar crown failure issues of deep-buried tunnels.

Collapse Behavior of Pipe-Framed Greenhouses with and without Reinforcement under Snow Loading:A 3-D Finite Element Analysis

The present paper first investigates the collapse behavior of a conventional pipe-framed greenhouse under snow loading based on a 3-D finite element analysis,in which both geometrical and material non-linearities are considered.Three snow load distribution patterns related to the wind-driven snow particle movement are used in the analysis.It is found that snow load distribution affects the deformation and collapse behavior of the pipe-framed greenhouse significantly.The results obtained in this study are consistent with the actual damage observed.Next,discussion is made of the effects of reinforcements by adding members to the basic frame on the strength of the whole structure,in which seven kinds of reinforcement methods are examined.A buckling analysis is also carried out.The results indicate that the most effective reinforcement method depends on the snow load distribution pattern.

Progressive Collapse Resistance of a New Staggered Story Isolated System

A new staggered isolated system developed from the mid-story isolated system is the new staggered story isolated system. There are not many studies on this structure currently. In this study, an 18-story new staggered story isolated system model is established using SAP2000. The dynamic nonlinear dynamic alternate method is used to analyze the structure against progressive collapse. Results show that the structure has good resistance to progressive collapse, and there is no progressive collapse under each working condition. The progressive collapse does not occur for the case of removing only one vertical structural member of the new staggered of isolated system. The side column has big influence on this isolated structures’ progressive collapse;the removal of vertical structural member of the isolation layer has less impact on the structure than the removal of the bottom vertical structural member. After the removing of the member, the internal force of the structure will be redistributed, and the axial force of the adjacent columns will change obviously, showing a trend of “near large and far small”.

施氮量对滴灌春小麦茎鞘NSC积累与转运的影响

在新疆气候条件下,为明确滴灌春小麦不同茎鞘节位果聚糖和NSC向籽粒转运提高产量的氮素响应机制,采用裂区试验设计,以强筋小麦‘新春37号’(XC37)、中筋小麦‘新春6号’(XC6)为主区,分别以施氮量300、255、210、0 kg·hm^(-2)为副区,研究施氮量对滴灌春小麦茎鞘不同节位(穗下节间、倒二节间、其余节间)果聚糖和非结构碳水化合物(NSC)的积累转运及其对产量贡献的影响。结果表明,随生育期的推进,两个品种春小麦茎鞘蔗糖果糖基转移酶(SST)活性、果聚糖含量、NSC含量及茎鞘干物质量呈先升后降的变化趋势,而果聚糖外水解酶(FEH)活性则为先降后升再降的趋势,各指标均以施氮量255 kg·hm^(-2)处理表现最优;各节位相比,其余节果聚糖代谢酶活性、果聚糖含量、NSC含量以及茎鞘干物质量最大,其果聚糖、NSC以及茎鞘干物质对产量贡献率分别比倒二节高11.18%~35.77%、14.77%~45.65%和25.81%~33.83%;两品种比较,XC37茎鞘中贮藏物质积累运转效率高于XC6,其果聚糖、NSC以及茎鞘干物质对产量贡献率分别比XC6高32.53%~116.74%、26.06%~35.26%和6.32%~21.73%;施氮量与品种互作效应对果聚糖、NSC花前转运率和贡献率及其穗下节干物质对产量贡献率均有显著影响。研究表明,果聚糖和NSC代谢及产量在施氮量为255 kg·hm^(-2)时表现最佳,该施氮量是新疆滴灌春小麦适宜的施氮水平。

Observation of the poloidally asymmetrical density perturbation of sawtooth collapse on J-TEXT

The detailed density perturbations provided by the advanced polarimeter-interferometer system(Polaris) during sawtooth collapse on the Joint Texas Experimental Tokamak(J-TEXT) are reported in this article.During a sawtooth collapse and the crash of plasma pressure at the center,it is found that the increase in density in the region between the inversion radius and mixing radius is poloidally asymmetrical,while the increase in temperature is poloidally symmetrical.The poloidal location where the density increases is dependent on the phase of the precursory m/n=1/1 kink mode.It is always out of phase with the hot core of the m/n=1/1 mode.The behaviors of density perturbations during sawtooth collapse observed in J-TEXT are beyond the expectations of the standard model,and this can shed new light on the understanding of sawtooth collapse.

Experimental and Numerical Study on Progressive Collapse Analysis of a Glulam Frame Structure:I.Side Column Exposed to Fire

This paper presents experimental and numerical investigations on progressive collapse behavior of a two-story glulam frame when the side column is exposed to ISO834 standard fire.The collapse mechanism initiated by fire is identified.The experimental results show that the progressive collapse of a glulam frame could be described for three stages,namely bending effect stage,catenary effect stage and failure stage,respectively.These stages are discussed in detail to understand the structural behavior before and during collapse.It is demonstrated that the entire frame slopes towards the side of the heated column,and the“overturning”collapse occurs eventually.The catenary effect of beams is the main reason for the progressive collapse of the frame.In addition,a finite element model of a glulam frame is established to simulate the progressive collapse behavior.The effects of axial loads on the columns are summarized.The numerical simulation results agree well with the experimental results,which could verify the effectiveness and practicability of finite element simulation.Furthermore,the progressive collapse resistance of the frame in practical design were proposed.

Slope Collapse Detection Method Based on Deep Learning Technology

Sofar,slope collapse detectionmainlydepends onmanpower,whichhas the followingdrawbacks:(1)lowreliability,(2)high risk of human safe,(3)high labor cost.To improve the efficiency and reduce the human investment of slope collapse detection,this paper proposes an intelligent detection method based on deep learning technology for the task.In thismethod,we first use the deep learning-based image segmentation technology to find the slope area from the captured scene image.Then the foreground motion detection method is used for detecting the motion of the slope area.Finally,we design a lightweight convolutional neural network with an attentionmechanismto recognize the detected motion object,thus eliminating the interference motion and increasing the detection accuracy rate.Experimental results on the artificial data and relevant scene data show that the proposed detection method can effectively identify the slope collapse,which has its applicative value and brilliant prospect.

Analysis on the Failure Causes of the Collapsed Tubing in an Oil Well

Due to the influence of multiple factors such as internal and external formation and mechanical pressure, medium corrosion and construction operation environment, a tubing collapse failure occurred in an oil well. In order to determine the failure cause of the tubing, physical and chemical tests and mechanical properties analysis were carried out on the failed tubing sample and the intact tubing. The results show that the chemical composition, ultrasonic and magnetic particle inspection, metallographic test, Charpy impact energy and external pressure mechanical property test of the failed tubing all meet the requirements of API Spec 5CT-2021 standard, but the yield strength of the failed tubing does not meet the requirements of API Spec 5CT-2021 standard. Through the analysis of the working conditions, it can be seen that the anti-extrusion strength of the tubing collapse does not meet the API 5C3 anti-extrusion strength standard. The failure type of the well tubing is tubing collapse caused by large internal and external pressure difference.

Custom Made Fenestrated Stent Graft Collapse after Thoracic Endovascular Aortic Repair: A Case Report

We present a case of stent graft collapse after performing thoracic endovascular aortic repair with a custom-made fenestrated stent graft. The patient was a 70-year-old woman with an asymptomatic aneurysm of the distal aortic arch, and thoracic endovascular aortic repair was performed. The patient showed a blood pressure difference between the left arm and the right arm on postoperative day (POD) 17 prompting the performance of a chest computed tomography scan which revealed stent graft collapse. She then underwent staged debranching of thoracic endovascular aortic repair. Stent graft collapse is a rare but well-described complication of thoracic endovascular repair. Therefore, patients who undergo such a procedure should be carefully monitored for signs and symptoms, which suggest the possibility of stent collapse.

Visualization of Vapor Film Collapse Behavior with Complexity on Quenching Process by Cellular Automaton

The vapor film collapse that occurs in the quenching process is complicated and affects the heat treatment quality and its distortion.In order to incorporate it into the MBD(Model Based Development)technology required these days,it is necessary to predict the quality of heat treatment by CAE(Computer Added Engineering),shorten the product development period.The calculation of the vapor film collapses in a simple and practical time in order to improve the product performance.However,in the past,in order to formulate the vapor film collapse on a simulation,it was necessary to perform a very large amount of computational calculation CFD(computational fluid dynamics),which was a problem in terms of computer resources and the model of vapor film collapse.In addition,this phenomenon has a complexity behavior of the phenomenon in iterative processing,which also complicates the calculation.In this study,the vapor film collapse phenomenon is easily visualized using self-organized cellular automaton simulation which includes the phenomena of“vapor film thickness and its fluctuation”,“flow disturbance”,“surface step of workpiece”,and“decrease of cooling due to r shape of surface”.The average cooling state and repeated fluctuations of the cooling state were reproduced by this method.

长江经济带岩溶塌陷分布、成因及其对工程建设的影响

[研究目的]岩溶塌陷是长江经济带主要环境地质问题之一,开展长江经济带岩溶塌陷分布、成因及其对工程建设的影响研究,对长江经济带立体综合交通走廊建设和新型城镇化建设地质环境安全保障具有重要意义。[研究方法]本文系统梳理了长江经济带岩溶塌陷研究成果,从岩溶环境特征、岩溶塌陷的成因类型和机制、发育及分布规律、对工程建设的影响及防控措施几个方面进行论述。[研究结果]研究结果表明,长江经济带岩溶塌陷易发区面积约25.4万km^(2),有记录的岩溶塌陷灾害2146处,矿山疏干排水、抽水、工程施工等是这一地区岩溶塌陷的主要诱发因素。长江经济带岩溶塌陷具有以下发育分布规律:一是具有区域性和地带性的分布特征;二是发育地层一般为均匀状纯碳酸盐岩,沿断裂破碎带、褶皱轴部裂隙发育带、可溶岩与非可溶岩接触地带等岩溶洞隙密集发育带分布;三是多发生在岩溶地下水强径流带、河流两岸等地下水位变化幅度大、水动力条件易发生急剧变化的地段;四是多发生在人类工程活动强烈的地区,塌陷的规模取决于人类活动的性质和强度。为了降低岩溶塌陷对工程建设和安全运营影响,本文同时提出了相关建议和对策。[结论]长江经济带岩溶塌陷分布广、危害大,高易发区内线性工程、重要城市群等规划建设应对这一环境地质问题引起重视,该研究成果可为长江经济带国土空间规划、地质灾害防治提供基础支撑和理论依据。

基于层次分析法与频率比模型的采空塌陷危险性评价

采空塌陷危险性评价是编制地质灾害防治规划、开展地质灾害防治与监测预警工作的重要依据。本文采用层次分析法与频率比模型相结合构建了采空塌陷危险性评价模型(AHP-PF组合模型)。以沈阳市蒲河-清水矿区为例,考虑了地质条件、地表特征、开采条件等3方面影响因素,选取了第四纪覆盖类型、第四纪松散层厚度、地质构造复杂程度、可采煤层顶板强度指标、煤层倾角、地表沉陷速率、采深采厚比、采空区叠置层数等8个评价指标,利用AHP-PF组合模型计算各指标权重及频率比,最后进行采空塌陷危险性分区。评价结果表明,采空塌陷危险性高区主要集中在采深采厚比小、沉陷速率大及目前仍在开采的区域,该区域是地质灾害防治、搬迁避让的重点区域。

古土壤层间富水对黄土场地湿陷性的影响

黄土地层中往往交替分布着多层红色古土壤层,由于其硬度相对较高,遇水不易湿陷,对黄土地层湿陷量的测量结果有很大的影响。然而,对于古土壤对湿陷量的控制作用目前还缺乏相关研究,导致对湿陷地层湿陷量的科学取舍缺乏理论依据。为此该文统计了黄土高原区的浸水试验结果,分析了不同区域湿陷量室内外差异特征,并以西安地区两个试验场地的大型浸水试验为研究对象,进行了不同试验条件下土体中水分的扩散、含水率的变化、土压力变化以及累计湿陷量等测量工作。最终结果表明,古土壤层的存在阻碍湿陷进程,在阻止水分下渗的同时,阻碍深部地层湿陷量传递至地表,使得湿陷量实测值与计算值之间的差异与古土壤层数呈现正相关的趋势。该文将为普遍存在古土壤的黄土地层自重湿陷机理的研究提供参考。

西山煤田岩溶陷落柱柱壁角特征研究及其意义

【意义】随着采掘向深部延伸,岩溶陷落柱已成为华北煤田矿井开采最隐蔽的致灾地质因素之一。柱壁角是描述陷落柱形态特征的重要指标之一。【方法和结果】以西山煤田上下煤层综采均切割过的265个岩溶陷落柱为数据源,通过分区、统计、函数构建、地质类比,挖掘西山煤田岩溶陷落柱柱壁角中蕴藏的地质信息,获得如下认识:(1)西山煤田陷落柱平均柱壁角为84.29°,超50%的陷落柱柱壁角在85°~90°,平均柱壁角自西北到东南逐渐增大,从82.05°增大到87.57°。(2)西山煤田陷落柱柱壁角与8号煤层揭露的陷落柱面积成反比,依据柱壁角与陷落柱面积大小关系,陷落柱划分为:充分塌陷陷落柱(90°~85°)、次充分塌陷陷落柱(<85°~81°)、不充分塌陷陷落柱(<81°);对应的2号煤层陷落柱面积分别为<556、556~1 700、>1 700 m^(2),等效半径为<13.3、13.3~23.3、>23.3 m;对应的8号煤层陷落柱面积分别为<1 250、1 250~2 750、>2 750 m^(2),半径分别为<20、20~30、>30 m。充分塌陷陷落柱一般发育有柱顶空腔,柱体结构较为松散,可导通顶板裂隙水与柱顶空腔水,以及底板承压奥灰水,是西山煤田导水陷落柱的一种重要类型。【结论】类比地质历史时期降雨量结果显示,具有“北柱南相”的西山煤田岩溶陷落柱,可能形成于气候湿热的古近纪渐新世时期,强烈的岩溶作用吸收了大气中的CO_(2),并锁定在沉积区,可能是导致全球大气CO_(2)含量急剧降低的重要原因。

中药塌渍治疗急性前循环缺血性脑卒中合并肺部感染

目的:探讨中药塌渍疗法治疗急性前循环缺血性脑卒中患者肺部感染的临床疗效。方法:选取2022年5月至2023年5月该院收治的93例前循环缺血性脑卒中患者进行前瞻性研究,排除院前肺部感染,按随机数字表法将其分成对照组和研究组。对照组采用常规的临床治疗方法,研究组则在对照组的基础上加用中药塌渍疗法进行治疗,经治疗后比较两组肺部感染评分表、不良反应发生率、神经功能缺陷评分表。结果:研究组患者治疗后肺部感染评分量表评分低于对照组患者,差异有统计学意义(P<0.05);研究组与对照组不良反应发生率差异无统计学意义(P>0.05);研究组与对照组神经功能缺损评分差异无统计学意义(P>0.05)。结论:中药塌渍治疗能有效缓解患者的急性前循环缺血性脑卒中合并肺部感染及相应的临床症状,促进患者病情康复,改善患者生活质量,值得在临床探索应用。

钓鱼台隧道洞口崩塌成因分析与处治措施研究

对池祁高速钓鱼台隧道洞口大型崩塌的成因进行了深入分析,提出了相应的崩塌处治措施,并通过现场监测分析对崩塌处治效果进行了验证。研究结果表明,钓鱼台隧道洞口崩塌是地形、地质、降雨、施工措施不当等多种因素共同作用的结果,其中地形和地质条件是形成崩塌的内因,降雨和施工措施不当等因素是诱发崩塌的外因。基于以上影响因素并结合隧道洞口实际情况,提出了“临时支护+永久性防护”的综合处治方案,即采用洞内背拱加固、掌子面反压回填、崩塌体卸载、洞口双层小导管支护、接长明洞以及洞顶仰坡锚杆框架防护的方案。现场巡视与监测数据表明,隧道洞口崩塌处治措施得当,工程效果显著,实现了预期目的。