[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomori...[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.[Result] The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification improved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl.[Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants.This study has provided foundation for the application of BSA in RAPD analysis.展开更多
Cynomorium is a herbaceous holoparasite that has been placed in Santalales, Saxifragales, Myrtales, or Sapindales. The inverted repeat （IR） region of the chloroplast genome region is slow evolving and, unlike mitoch...Cynomorium is a herbaceous holoparasite that has been placed in Santalales, Saxifragales, Myrtales, or Sapindales. The inverted repeat （IR） region of the chloroplast genome region is slow evolving and, unlike mitochondrial genes, the chloroplast genome experiences few horizontal gene transfers between the host and parasite. Thus, in the present study, we used sequences of the IR region to test the phylogenetic placements of Cynomorium. Phylogenetic analyses of the chloroplast IR sequences generated largely congruent ordinal relationships with those from previous studies of angiosperm phylogeny based on single or multiple genes. Santalales was closely related to Caryophyllales and asterids. Saxifragales formed a clade where Peridiscus was sister to the remainder of the order, whereas Paeonia was sister to the woody clade of Saxifragales. Cynomorium is not closely related to Santalales, Saxifragales, Myrtales, or Sapindales; instead, it is included in Rosales and sister to Rosaceae. The various placements of the holoparasite on the basis of different regions of the mitochondrial genome may indicate the heterogeneous nature of the genome in the parasite. However, it is unlikely that the placement of Cynomorium in Rosales is the result of chloroplast gene transfer because Cynomorium does not parasitize on rosaceous plants and there is no chloroplast gene transfer between Cynomorium and Nitraria, a confirmed host of Cynomorium and a member of Sapindales.展开更多
From the whole plant of Cynomorium songaricum a new triterpene was isolated together with acetyl ursolic acid, ursolic acid, β-sitosterol palmitate, β-sitosterol, palmitic acid and β-sitosterol glucoside. The struc...From the whole plant of Cynomorium songaricum a new triterpene was isolated together with acetyl ursolic acid, ursolic acid, β-sitosterol palmitate, β-sitosterol, palmitic acid and β-sitosterol glucoside. The structure of the new compound was elucidated as ursa-12-ene-28-oic acid, 3β-propanedioic acid monoester (cynoterpene, 1).展开更多
The search for possible effective local therapeutic agents led to the discovery of a plant that was later known as Fungus Melitensis.This parasitic flowering plant was initially believed to grow only on a small islet ...The search for possible effective local therapeutic agents led to the discovery of a plant that was later known as Fungus Melitensis.This parasitic flowering plant was initially believed to grow only on a small islet off Gozo known variably as General's or Fungus Rock.It is now known to be more widely distributed with a range extending from the Canary Islands to China.First mentioned in 1647 by the Maltese historian Gian Francesco Abela,the plant was later described and illustrated in 1674 by the Palermo botanist Paolo Boccone,while a detailed clinical treatise was prepared in 1689 by the Maltese physician Gio Francesco Bonamico.Based on the principles of the“doctrine of signatures,”the plant was considered useful by virtue of its color in conditions involving bleeding,while on the basis of the phallic appearance,it was considered efficacious for venereal disease.The medicinal properties of the plant became renowned throughout the European continent,increasing the demand for its collection and export.Measures were introduced to limit the collection to authorized individuals while physical access to the islet was made more difficult by cutting away the sloping parts of the islet.The plant lost its medicinal reputation during the early decades of the 19^th century and has now been relegated to the annals of medical history and folklore,though it is still designated a protected species.展开更多
基金Supported by the Knowledge Innovation Project of Northwest Institute of Plateau Biology of Chinese Academy of Sciences(CXLY-2002-7)~~
文摘[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.[Result] The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification improved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl.[Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants.This study has provided foundation for the application of BSA in RAPD analysis.
基金supported,in part,by a scholarship from the China Scholarship Council to ZZ
文摘Cynomorium is a herbaceous holoparasite that has been placed in Santalales, Saxifragales, Myrtales, or Sapindales. The inverted repeat （IR） region of the chloroplast genome region is slow evolving and, unlike mitochondrial genes, the chloroplast genome experiences few horizontal gene transfers between the host and parasite. Thus, in the present study, we used sequences of the IR region to test the phylogenetic placements of Cynomorium. Phylogenetic analyses of the chloroplast IR sequences generated largely congruent ordinal relationships with those from previous studies of angiosperm phylogeny based on single or multiple genes. Santalales was closely related to Caryophyllales and asterids. Saxifragales formed a clade where Peridiscus was sister to the remainder of the order, whereas Paeonia was sister to the woody clade of Saxifragales. Cynomorium is not closely related to Santalales, Saxifragales, Myrtales, or Sapindales; instead, it is included in Rosales and sister to Rosaceae. The various placements of the holoparasite on the basis of different regions of the mitochondrial genome may indicate the heterogeneous nature of the genome in the parasite. However, it is unlikely that the placement of Cynomorium in Rosales is the result of chloroplast gene transfer because Cynomorium does not parasitize on rosaceous plants and there is no chloroplast gene transfer between Cynomorium and Nitraria, a confirmed host of Cynomorium and a member of Sapindales.
文摘From the whole plant of Cynomorium songaricum a new triterpene was isolated together with acetyl ursolic acid, ursolic acid, β-sitosterol palmitate, β-sitosterol, palmitic acid and β-sitosterol glucoside. The structure of the new compound was elucidated as ursa-12-ene-28-oic acid, 3β-propanedioic acid monoester (cynoterpene, 1).
文摘The search for possible effective local therapeutic agents led to the discovery of a plant that was later known as Fungus Melitensis.This parasitic flowering plant was initially believed to grow only on a small islet off Gozo known variably as General's or Fungus Rock.It is now known to be more widely distributed with a range extending from the Canary Islands to China.First mentioned in 1647 by the Maltese historian Gian Francesco Abela,the plant was later described and illustrated in 1674 by the Palermo botanist Paolo Boccone,while a detailed clinical treatise was prepared in 1689 by the Maltese physician Gio Francesco Bonamico.Based on the principles of the“doctrine of signatures,”the plant was considered useful by virtue of its color in conditions involving bleeding,while on the basis of the phallic appearance,it was considered efficacious for venereal disease.The medicinal properties of the plant became renowned throughout the European continent,increasing the demand for its collection and export.Measures were introduced to limit the collection to authorized individuals while physical access to the islet was made more difficult by cutting away the sloping parts of the islet.The plant lost its medicinal reputation during the early decades of the 19^th century and has now been relegated to the annals of medical history and folklore,though it is still designated a protected species.
