在金电极表面制备了DTNB(5,5′ Di thiobis(2 nitrobenzoicacid))自组装单分子层膜(DTNB/AuSAM)。多巴胺在DTNB自组装膜上有一对可逆性良好的氧化还原峰,其氧化峰电流与多巴胺的浓度在5.0×10-6mol/L～1.0×10-4mol/L的范围内...在金电极表面制备了DTNB(5,5′ Di thiobis(2 nitrobenzoicacid))自组装单分子层膜(DTNB/AuSAM)。多巴胺在DTNB自组装膜上有一对可逆性良好的氧化还原峰,其氧化峰电流与多巴胺的浓度在5.0×10-6mol/L～1.0×10-4mol/L的范围内呈线性关系,检出限为1.0×10-6mol/L。在pH3.5的缓冲溶液中,在DTNB自组装膜上多巴胺和抗坏血酸的电化学响应可以明显区分,氧化峰电位分离达276mV。可用于抗坏血酸存在下多巴胺的检测。测定了盐酸多巴胺注射液中多巴胺的含量,其平均回收率为104%。展开更多
Objective To study the association of DTNBP1 gene with some symptom factors of schizophrenia.Methods A total of 285 unrelated schizophrenic individuals were recruited from December 2004 to January 2009 for genetic ana...Objective To study the association of DTNBP1 gene with some symptom factors of schizophrenia.Methods A total of 285 unrelated schizophrenic individuals were recruited from December 2004 to January 2009 for genetic analysis,and their symptom factors were assessed based on the Positive and Negative Syndrome Scale(PANSS).The quantitative trait test was performed by the UNPHASED program(version 3.0.12) to investigate the association between scored positive and negative symptoms and the single nucleotide polymorphisms(SNPs) in DTNBP1 gene.Results The quantitative trait test showed allelic association of rs909706 with the excitement symptom of schizophrenia(P<0.05,adjusted by 10 000 permutations),while the genotype C/G of rs2619539 with a negative symptom,lack of spontaneity and flow of conversation(P<0.05,adjusted by 10 000 permutations).Conclusion DTNBP1 variations are possibly associated with some symptoms of schizophrenia,which could partly explain the relationship between the susceptibility gene DTNBP1 and that disease.展开更多
A sensitive, micro-5, 5' -dithiobis-2 -nitrobenzise acid (DTNB) assay for determination of glutathione peroxidase (GSH-Px) in mouse blood was reported, Trichloroacetic acid (TCA) 0.16 mol/L was selected as a prote...A sensitive, micro-5, 5' -dithiobis-2 -nitrobenzise acid (DTNB) assay for determination of glutathione peroxidase (GSH-Px) in mouse blood was reported, Trichloroacetic acid (TCA) 0.16 mol/L was selected as a protein pricipitation reagent instead of metaphosphoric acid to remove protein in 10 micro liter blood sample.The surplus glutathione (GSH) reacted with DTNB to form a pale yellow color at 37℃ in pH 6.5 solution. There is good linearity between GSH concenlration and absorbance of pale yellow color measured at 423 nm (r=0.9973). Precision studies for both within day and day-to-day at different concenlrations of DTNB provided CV values of less than 5%. Two kinds of selenium (Se) rich yeast were given to mice (500μg/kg) once a day for ten days by oral abministration. The activity of GSH-Px was measured according to direct DTNB assay. There is high significant difference between treated and control groups. The results show that blood GSH-Px level can be used as an indicator of Se status and the capability of scavenging peroxidatives in mammals.展开更多
文摘在金电极表面制备了DTNB(5,5′ Di thiobis(2 nitrobenzoicacid))自组装单分子层膜(DTNB/AuSAM)。多巴胺在DTNB自组装膜上有一对可逆性良好的氧化还原峰,其氧化峰电流与多巴胺的浓度在5.0×10-6mol/L～1.0×10-4mol/L的范围内呈线性关系,检出限为1.0×10-6mol/L。在pH3.5的缓冲溶液中,在DTNB自组装膜上多巴胺和抗坏血酸的电化学响应可以明显区分,氧化峰电位分离达276mV。可用于抗坏血酸存在下多巴胺的检测。测定了盐酸多巴胺注射液中多巴胺的含量,其平均回收率为104%。
基金Supported by National High Technology Research and Development Program of China (863 Program) (2006AA02A407)National Basic Research Program (973 Program) (2010CB529603)Beijing Natural Science Foundation (7102109)
文摘Objective To study the association of DTNBP1 gene with some symptom factors of schizophrenia.Methods A total of 285 unrelated schizophrenic individuals were recruited from December 2004 to January 2009 for genetic analysis,and their symptom factors were assessed based on the Positive and Negative Syndrome Scale(PANSS).The quantitative trait test was performed by the UNPHASED program(version 3.0.12) to investigate the association between scored positive and negative symptoms and the single nucleotide polymorphisms(SNPs) in DTNBP1 gene.Results The quantitative trait test showed allelic association of rs909706 with the excitement symptom of schizophrenia(P<0.05,adjusted by 10 000 permutations),while the genotype C/G of rs2619539 with a negative symptom,lack of spontaneity and flow of conversation(P<0.05,adjusted by 10 000 permutations).Conclusion DTNBP1 variations are possibly associated with some symptoms of schizophrenia,which could partly explain the relationship between the susceptibility gene DTNBP1 and that disease.
文摘A sensitive, micro-5, 5' -dithiobis-2 -nitrobenzise acid (DTNB) assay for determination of glutathione peroxidase (GSH-Px) in mouse blood was reported, Trichloroacetic acid (TCA) 0.16 mol/L was selected as a protein pricipitation reagent instead of metaphosphoric acid to remove protein in 10 micro liter blood sample.The surplus glutathione (GSH) reacted with DTNB to form a pale yellow color at 37℃ in pH 6.5 solution. There is good linearity between GSH concenlration and absorbance of pale yellow color measured at 423 nm (r=0.9973). Precision studies for both within day and day-to-day at different concenlrations of DTNB provided CV values of less than 5%. Two kinds of selenium (Se) rich yeast were given to mice (500μg/kg) once a day for ten days by oral abministration. The activity of GSH-Px was measured according to direct DTNB assay. There is high significant difference between treated and control groups. The results show that blood GSH-Px level can be used as an indicator of Se status and the capability of scavenging peroxidatives in mammals.
