AIM To study the effects of Dangshen [dried root of Codonopsis Pilosula (Franch) Nannf] on contractile activity of isolated gastric muscle strips in rats and its possible mechanism involved.METHODS Each isolated gastr...AIM To study the effects of Dangshen [dried root of Codonopsis Pilosula (Franch) Nannf] on contractile activity of isolated gastric muscle strips in rats and its possible mechanism involved.METHODS Each isolated gastric muscle strip was put in a tissue chamber containing 5ml Krebs solution, constantly warmed by water jacket at 37℃ and supplied with a mixed gas of 95% O2 and 5% CO2. After incubating for 1h with 1g tension, Dangshen of varied concentration was added cumulatively in the tissue chamber at intervals of 2 minutes. The isometrical response was measured on ink-writing recorders.RESULTS Dangshen dose dependence increased the resting tension of longitudinal muscle (LM) of fundus (r=0.96, P＜0.01), the mean contractile amplitude of circular muscle (CM) of the stomach body (r=0.87, P＜0.05) and CM of antrum (r=0.98, P＜0.01), and the motility index CM of pylorus (r=0.87, P＜0.05). Atropine (5×10-8mol/L) or Hexamethonium (10-5mol/L) or Indomethacin (5×10-7mol/L) was given 2 minutes before the administration of Dangshen, it did not abolish its dose related manner. Atropine apparently reduced the increasing action of 10% and 30% Dangshen on the resting tesion of LM of fundus (P＜0.05), 30%, 100% and 200% Dangshen on bodied strips (P＜0.05), 100% and 200% Dangshen on antral strips (P＜0.05). Hexamethonium reduced the increasing action of 10% and 30% Dangshen on the resting tesion of LM of fundus (P＜0.05 and P＜0.05), 30%, 100% and 200% Dangshen on bodied strips (P＜0.05), and 100% and 200% Dangshen on pyloric strips (P＜0.05). Indomethacin inhibited the effect of 10% Dangshen on the resting tesion of LM of fundus (P＜0.05), but did not affect the exciting action of Dangshen on strips of body, antrum and pylorus.CONCLUSION The results showed that Dangshen possessed exciting action on the isolated gastric smooth muscle strips of the rat. The exciting action of Dangshen was partially mediated via cholinergic M and N receptors.展开更多
Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods:...Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.展开更多
Wash the red dates, astragalus root, and dangshen.Draw the chicken, and wash the lean pork.Add water to the pot, add the chicken and lean pork, bring to the boil, and pour off the water.
目的:观察黄芪、党参提取物对紫杉醇抑制Lewis肺癌血管生成和肿瘤转移的增强作用,为中医药抗肿瘤应用提供实验依据。方法:C57BL/6小鼠右肋处皮下接种Lewis肺癌,6h后随机分为实验组、紫杉醇组和对照组。实验组接受紫杉醇联合参芪注射液治...目的:观察黄芪、党参提取物对紫杉醇抑制Lewis肺癌血管生成和肿瘤转移的增强作用,为中医药抗肿瘤应用提供实验依据。方法:C57BL/6小鼠右肋处皮下接种Lewis肺癌,6h后随机分为实验组、紫杉醇组和对照组。实验组接受紫杉醇联合参芪注射液治疗,紫杉醇组接受同等剂量的紫杉醇治疗,对照组用等体积的生理盐水代替。观察指标:移植瘤体积,肿瘤组织内微血管度和肺脏转移瘤数量,小鼠存活时间。结果:与紫杉醇治疗相比,紫杉醇联合参芪可以明显减少移植瘤内的微血管密度(10.1±4.4 vs 16.8±7.3,P<0.05)和肺脏转移瘤个数(13.4±4.3 vs 18.4±3.9,P<0.05),明显延长小鼠的存活时间(43.3±8.4d vs 36.3±6.6d,P<0.05)。结论:黄芪、党参提取物对紫杉醇抑制肿瘤血管生成和移植肿瘤转移有一定的增强作用。展开更多
文摘AIM To study the effects of Dangshen [dried root of Codonopsis Pilosula (Franch) Nannf] on contractile activity of isolated gastric muscle strips in rats and its possible mechanism involved.METHODS Each isolated gastric muscle strip was put in a tissue chamber containing 5ml Krebs solution, constantly warmed by water jacket at 37℃ and supplied with a mixed gas of 95% O2 and 5% CO2. After incubating for 1h with 1g tension, Dangshen of varied concentration was added cumulatively in the tissue chamber at intervals of 2 minutes. The isometrical response was measured on ink-writing recorders.RESULTS Dangshen dose dependence increased the resting tension of longitudinal muscle (LM) of fundus (r=0.96, P＜0.01), the mean contractile amplitude of circular muscle (CM) of the stomach body (r=0.87, P＜0.05) and CM of antrum (r=0.98, P＜0.01), and the motility index CM of pylorus (r=0.87, P＜0.05). Atropine (5×10-8mol/L) or Hexamethonium (10-5mol/L) or Indomethacin (5×10-7mol/L) was given 2 minutes before the administration of Dangshen, it did not abolish its dose related manner. Atropine apparently reduced the increasing action of 10% and 30% Dangshen on the resting tesion of LM of fundus (P＜0.05), 30%, 100% and 200% Dangshen on bodied strips (P＜0.05), 100% and 200% Dangshen on antral strips (P＜0.05). Hexamethonium reduced the increasing action of 10% and 30% Dangshen on the resting tesion of LM of fundus (P＜0.05 and P＜0.05), 30%, 100% and 200% Dangshen on bodied strips (P＜0.05), and 100% and 200% Dangshen on pyloric strips (P＜0.05). Indomethacin inhibited the effect of 10% Dangshen on the resting tesion of LM of fundus (P＜0.05), but did not affect the exciting action of Dangshen on strips of body, antrum and pylorus.CONCLUSION The results showed that Dangshen possessed exciting action on the isolated gastric smooth muscle strips of the rat. The exciting action of Dangshen was partially mediated via cholinergic M and N receptors.
基金Guangdong Science and Technology Department,Grant/Award Number:2015A030302073
文摘Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.
文摘Wash the red dates, astragalus root, and dangshen.Draw the chicken, and wash the lean pork.Add water to the pot, add the chicken and lean pork, bring to the boil, and pour off the water.
文摘目的:观察黄芪、党参提取物对紫杉醇抑制Lewis肺癌血管生成和肿瘤转移的增强作用,为中医药抗肿瘤应用提供实验依据。方法:C57BL/6小鼠右肋处皮下接种Lewis肺癌,6h后随机分为实验组、紫杉醇组和对照组。实验组接受紫杉醇联合参芪注射液治疗,紫杉醇组接受同等剂量的紫杉醇治疗,对照组用等体积的生理盐水代替。观察指标:移植瘤体积,肿瘤组织内微血管度和肺脏转移瘤数量,小鼠存活时间。结果:与紫杉醇治疗相比,紫杉醇联合参芪可以明显减少移植瘤内的微血管密度(10.1±4.4 vs 16.8±7.3,P<0.05)和肺脏转移瘤个数(13.4±4.3 vs 18.4±3.9,P<0.05),明显延长小鼠的存活时间(43.3±8.4d vs 36.3±6.6d,P<0.05)。结论:黄芪、党参提取物对紫杉醇抑制肿瘤血管生成和移植肿瘤转移有一定的增强作用。
