Isolation,Screening and Identification of Cellulose Decomposing Strains in Straw-amended Soil

[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.

Isolating and Screening of Effective Decomposing Strain of Silkworm Chrysalis Protein and Study on Parts of Its Enzymatic Properties

[Objective] The research aimed to obtain an effectively-decomposing strain of silkworm chrysalis protein and discuss its enzymatic properties.[Method] The effectively-decomposing bacteria of protein was isolated from the decayed silkworm chrysalis by using dilution plate and its enzymatic properties were tested after primary screening and second screening.The enzyme activity was determined and the intermediate and small molecule protein content in silkworm chrysalis was measured after solid-state fermentati...

Screening on oil-decomposing microorganisms and application in organic waste treatment machine

As an oil-decomposable mixture of two bacteria strains(Bacillus sp. and Pseudomonas sp.), Y3 was isolated after 50 d domestication under the condition that oil was used as the limited carbon source. The decomposing rate by Y3 was higher than that by each separate individual strain, indicating a synergistic effect of the two bacteria. Under the conditions that T=25—40℃,pH=6—8, HRT(Hydraulic retention time)=36 h and the oil concentration at 0.1%, Y3 yielded the highest decomposing rate of 95.7 %. Y3 was also applied in an organic waste treatment machine and a certain rate of activated bacteria was put into the stuffing. A series of tests including humidity, pH, temperature, C/N rate and oil percentage of the stuffing were carried out to check the efficacy of oil-decomposition. Results showed that the oil content of the stuffing with inoculums was only half of that of the control. Furthermore, the bacteria were also beneficial to maintain the stability of the machine operating. Therefore, the bacteria mixture as well as the machines in this study could be very useful for waste treatment.

Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)

We collected the diseased blades of Larninaria japonica from Yantai Sea Farm from October to December 2002, and the alginic acid decomposing bacterium on the diseased blade was isolated and purified, and was identified as A lteromonas espejiana. This bacterium was applied as the causative pathogen to infect the blades of L. japonica under laboratory conditions. The aim of the present study was to identify the effects of the bacterium on the growth of L. japonica, and to find the possibly effective mechanism. Results showed that： （1）The blades of L.japonica exhibited symptoms of lesion,bleaching and deteriortion when infected by the bacterium. and their growth and photosynthesis were dramatically suppressed. At the same time, the reactive oxygen species （ROS） generation enhanced obviously, and the relative membrane permeability increased significantly. The contents of malonaldehyde （MDA） and free fatty acid in the microsomol membrane greatly elevated, but the phospholipid content decreased. Result suggested an obvious peroxidation and deesterrification in the blades of L. japonica when infected by the bacterium. （2） The simultaneous assay on the antioxidant enzyme activities demonstrated that superoxide dismutase （SOD） and catalase （CAT） increased greatly when infected by the bacterium, but glutathione peroxidase （Gpx） and ascorbate peroxidase （APX） did not exhibit active responses to the bacterium throughout the experiment. （3） The histomorphological observations gave a distinctive evidence of the severity of the lesions as well as the relative abundance in the bacterial population on the blades after infection. The bacterium firstly invaded into the endodermis of L. japonica and gathered around there, and then resulted in the membrane damage, cells corruption and ultimately, the death of L. japonica.

Stimulatory effects of biosurfactant produced by Pseudomonas aeruginosa BSZ-07 on rice straw decomposing

Biosurfactant,produced by Pseudomonas aeruginosa BSZ-07,was added to the dee straw decomposing process to enhance the production of reducing sugars.Observed by Fourier Transform InfraRed(FT-IR)and Nuclear Magnetic Resonance(NMR)analysis, the purified biosurfactant was considered as a mixture of RL1 and RL2,which are two different types of rhamnolipids.Two different adding methods,adding the purified rhamnolipid and the on-site production of it were compared.The results showed that 0.5 g/L was the optimum co...

Preparation of nanostructure silver powders by mechanical decomposing and mechanochemical reduction of silver oxide

The mechanical decomposing and mechanochemical reductions of silver oxide for preparation of nanocrystalline silver powders by high planetary ball mill was investigated. XRD and HRSEM techniques were used to characterize the structural evolution and morphological changes of products. The results show that the nanostructured silver with an average crystallite size of 14 nm and internal strain of 0.75% is synthesized by mechanical decomposing of Ag2O after 95 h milling. While, the product of mechanochemical reduction of silver oxide using graphite after 22 h milling is nanostructured silver with an average crystallite size of 28 nm and internal strain of 0.44%.

An Approach for Improving Short-Term Prediction of Summer Rainfall over North China by Decomposing Interannual and Decadal Variability

A statistical downscaling approach was developed to improve seasonal-to-interannual prediction of summer rainfall over North China by considering the effect of decadal variability based on observational datasets and dynamical model outputs.Both predictands and predictors were first decomposed into interannual and decadal components.Two predictive equations were then built separately for the two distinct timescales by using multivariate linear regressions based on independent sample validation.For the interannual timescale,850-hPa meridional wind and 500-hPa geopotential heights from multiple dynamical models＇ hindcasts and SSTs from observational datasets were used to construct predictors.For the decadal timescale,two well-known basin-scale SST decadal oscillation （the Atlantic Multidecadal Oscillation and the Pacific Decadal Oscillation） indices were used as predictors.Then,the downscaled predictands were combined to represent the predicted/hindcasted total rainfall.The prediction was compared with the models＇ raw hindcasts and those from a similar approach but without timescale decomposition.In comparison to hindcasts from individual models or their multi-model ensemble mean,the skill of the present scheme was found to be significantly higher,with anomaly correlation coefficients increasing from nearly neutral to over 0.4 and with RMSE decreasing by up to 0.6 mm d-1.The improvements were also seen in the station-based temporal correlation of the predictions with observed rainfall,with the coefficients ranging from-0.1 to 0.87,obviously higher than the models＇ raw hindcasted rainfall results.Thus,the present approach exhibits a great advantage and may be appropriate for use in operational predictions.

An initial study of insect succession on decomposing rabbit carrions in Harare,Zimbabwe

Objective:To investigate insects visiting sun exposed and shaded decomposing rabbit carcasses and to establish the relationship between insects and carcasses which may be of forensic importance in Harare.Methods:Two rabbits weighing 2.3 kg and 2.5 kg were killed by sharp blows on the head.One was exposed to the sun while the other was placed under shade.The carcasses were allowed to decompose and insects were collected twice a day for the first week and thereafter once a day up to the end of the 7 weeks.Maggots were also collected from the decomposing carcasses and reared.Results:Five dipteran families(Galliphoridae,Muscidae,Sarcophagidae,Phoridae and Drosophilidae)were identified from the sun-exposed carcass.Species collected included Lucilia cuprina(L.cuprina),Chrysomya albiceps(C.albiceps),Musca domestica,Sarcophaga sp.and Drosophila sp.Four families(Calliphoridae,Muscidae,Phoridae,Anthomyiidae)were identified from the shaded carcass.Representatives of these families included L cuprina,C.albiceps,Musca domestica,and Hydrotaea sp.Three Coleopteran families(Histeridae,Cleridae and Dermestidae)were identified from hodt carcasses.The observed species were Saprinus sp,Necrobia rufipes and Dermestes sp.Fomicidae(Hymenoptera)was represented by only one species(Pheidoie sp.)Flies which emerged from the rearing units were L cuprina,Lucilia sp.,C.albiceps,Sarcophaga sp.and Sepsis sp.).Conclusions:Of the dipteran species collected during the study,L cuprina and C.albiceps could be important for further forensic studies since they were collected from the carcasses and also observed from the rearing units.

Short-range structural change in indium melt by Gaussian peaks decomposing of RDF

Liquid indium's structure was studied at 280, 390, 550, 650, and 750 deg Crespectively by using an elevated temperature X-ray diffractometer, and its radial distributionfunction (RDF) at different temperatures was decomposed into 4 Gaussian peaks in the range of0.2-0.6nm. Positions of the decomposed Gaussian peaks were compared with the nearest and the secondnearest neighbor atomic distances, respectively. It is shown that the position of the firstdecomposed Gaussian peak is similar to the nearest neighbor atomic distance in liquid In at thecorresponding temperature, and that of the third decomposed Gaussian peak is similar to the secondnearest neighbor atomic distance. Moreover, the first and the third Gaussian peaks correspond to thefirst and the second atom shells of liquid In at the corresponding temperatures, respectively.Therefore, the position and the area of Gaussian peaks can represent the position and atom number ofcorresponding shells. Based on this result, short-range structural changes in liquid In wasstudied. It was found that the first and the second shells are close to the referred atom, and theatom number at the shells decreases with the increasing temperature from 280 to 750 deg C. Indifferent ranges of temperature, structural changes in the first and the second shells showdifferent features.

The effects of the environmental factors on Laminaria disease caused by alginic acid decomposing bacteria

-The rot disease of Laminaria occurs often in nurseries of sporeling and commercial cultivation in the sea and results in economic loss greatly. Usually the disease outbreaks accompanied with massive multiplication of alginic acid decomposing bacteria. From the section of the decaying Lamuutria which resulted from the inoculation of alginic acid decomposing bacteria, it was observed that the bacteria invaded the epiderm of the Laminaria surface at first, then entered the ex-odermis, endodermis and pith. In addition, there were a great amount of bacteria in the intercellular region and a lot of free cells of the algae in the decaying areas. The wall of some free cells was decomposed, which led to soft tissue or disintegration. Alginic acid decomposing bacteria are normal epiphytic microorganisms growing on Lamuutria surface. These bacteria do not cause disease at normal environmental conditions. The experiments showed that the unfavourable conditions, e. g. , wounding, overcrowding, high temperature reduced the ability of antibacterial activity and made the algae more susceptible to the pathogens and favoured the multiplication of alginic acid decomposing bacteria and finally led to the disease outbreak. The unfavourable environmental factors which resulted from a variety of reasons were the main cause of the disease.

Screening of cellulose decomposing fungi in sandy dune soil of Horqin Sandy Land

Cellulose decomposing fungi play an important role in litter decomposition and are decisive in nutrient cycling in sandy land ecosystems. Thirty-one strains were isolated to select efficient cellulose decomposers, and four efficient cellulose decomposing fungi （NM3-1, NM3-2, NM3-3, and NM3-4） were screened using a CMC （carboxymethyl cellulose） carbon source in dune soil of Horqin Sandy Land. They were identified as Asperigillus calidoustus, Fusarium oxysporum, Fusarium solani, and Hypocrea lixii by rDNA-ITS molecular biological methods. Cloth decomposition rates were 15.71%, 15.89%, 17.29%, and 17.89% by the four efficient decomposers incubated for 30 days, respectively. Screening of efficient cellulose decomposers can not only increase the dune soil functional microbe bank, but can also accelerate litter decom- position and available nutrient input in the Horqin Sandy Land.

Vertical distribution of phosphorus and P-dissolving/ decomposing bacteria in the sediment of Guanting reservoir

In this study, we determined pH, phosphorus content and the number of P-dis- solving/decomposing bacteria in relation to the depth in the sediment of Guanting reservoir in Beijing. The pH value was slightly increased from 8.0 in the surface to 8.5 in the bottle (69 cm in depth) of sediment. The highest P content, 1269 mg/kg, was detected in the 35 cm layer, followed by the 5 cm (993 mg/kg) and 69 cm (580 mg/kg) layers. The number of inorganic P-dissolving bacteria varied from 6(102 to 8(104 and the organic P-discomposing bacteria were from 1.9(103 to 6.3(104 per gram sediment in different depths, which were counted under 28℃ and 20℃ ( pH7.5 and 8.5. The number of P-dissolving/discomposing bacteria was directly correlated to the P content in each layer of sediments. The analysis of P-dissolving/decomposing ability of bacteria showed that some of these bacteria were also capable of accumulating P intracellularly. The intracellular P-accumulation was more efficient at lower temperature; in contrast, the activity of P-dissolving/decomposing was stronger at higher temperature. So the content of dissolved P in water body, or quality of water, could be affected by the change of temperature via the regulation of bacterial activity. On the basis of 16S rDNA sequencing, the 13 efficient P-dis- solving/decomposing bacteria were identified as Bacillus spp., Bacterium sp., Microbacterium sp., Paenibacillus sp. and Pseudomonas sp.

Gaussian peaks decomposing of simple liquids' RDF

According to feasibility of Gaussian peaks decomposing distribution function (DF) 4πrρ(r), radial distribution function (RDF) 4πr 2ρ(r) of simple liquids also is possibly decomposed into Gaussian peaks withr in the size of several atoms. Experimental structure parameters of pure liquid Al and Si were obtained by θ-θ X-ray diffraction measurements and following data processing. Based on Gaussian decomposing liquid aluminum’s DF 4πrρ(r), liquid aluminum and silicon’s radial distribution function (RDF) 4πr 2ρ(r), it is shown that RDF of simple liquids is able to be decomposed in Gaussian function, compared with decomposing of DF, supplying more physical meaning and better indicating the short range order of liquids, which draws a clearer structure picture of simple liquids.

Image decomposing for inpainting using compressed sensing in DCT domain

Inpainting images with occlusion or corruption is a challenging task. Most existing algorithms are pixel based, which construct a statistical model from image features. However, in these algorithms, the frequency component is not sufficiently addressed. In this paper, we propose a novel algorithm that utilizes compressed sensing （CS） in frequency domain to reconstruct corrupted images. In order to reconstruct image, we first decompose the image into two functions with different basic characteristics - structure component and textual component. We seek a sparse representation for the functions and use the DCT coefficients of this representation to generate an over-complete dictionary. Experimental results on real world datasets demonstrate the efficacy of our method in image inpainting. We compare our method with three state-of-the-art inpalnting algorithms and demonstrate its advantages in terms of both quantitative and qualitative aspects.

Variational Mode Decomposition-Informed Empirical Wavelet Transform for Electric Vibrator Noise Analysis

Electric vibrators find wide applications in reliability testing, waveform generation, and vibration simulation, making their noise characteristics a topic of significant interest. While Variational Mode Decomposition (VMD) and Empirical Wavelet Transform (EWT) offer valuable support for studying signal components, they also present certain limitations. This article integrates the strengths of both methods and proposes an enhanced approach that integrates VMD into the frequency band division principle of EWT. Initially, the method decomposes the signal using VMD, determining the mode count based on residuals, and subsequently employs EWT decomposition based on this information. This addresses mode aliasing issues in the original method while capitalizing on VMD’s adaptability. Feasibility was confirmed through simulation signals and ultimately applied to noise signals from vibrators. Experimental results demonstrate that the improved method not only resolves EWT frequency band division challenges but also effectively decomposes signal components compared to the VMD method.

Litter quality and decomposer complexity co-drive effect of drought on decomposition

Litter decomposition is key to ecosystem carbon(C)and nutrient cycling,but this process is anticipated to weaken due to projected more extensive and prolonged drought.Yet how litter quality and decomposer community complexity regulate decomposition in response to drought is less understood.Here,in a five-year manipulative drought experiment in a Masson pine forest,leaf litter from four subtropical tree species(Quercus griffthii Hook.f.&Thomson ex Miq.,Acacia mangium Willd.,Pinus massoniana Lamb.,Castanopsis hystrix Miq.)representing different qualities was decomposed for 350 d in litterbags of three different mesh sizes(i.e.,0.05,1,and 5 mm),respectively,under natural conditions and a 50%throughfall rain exclusion treatment.Litterbags of increasing mesh sizes discriminate decomposer communities(i.e.,microorganisms,microorganisms and mesofauna,microorganisms and meso-and macrofauna)that access the litter and represent an increasing complexity.The amount of litter C and nitrogen(N)loss,and changes in their ratio(C/N_(loss)),as well as small and medium-sized decomposers including microorganisms,nematodes,and arthropods,were investigated.We found that drought did not affect C and N loss but decreased C/N_(loss)(i.e.,decomposer N use efficiency)of leaf litter irrespective of litter quality and decomposer complexity.However,changes in the C/N_(loss)and the drought effect on C loss were both dependent on litter quality,while drought and decomposer complexity interactively affected litter C and N loss.Increasing decomposer community complexity enhanced litter decomposition and allowing additional access of meso-and macro-fauna to litterbags mitigated the negative drought effect on the microbial-driven decomposition.Furthermore,both the increased diversity and altered trophic structure of nematode due to drought contributed to the mitigation effects via cascading interactions.Our results show that litter quality and soil decomposer community complexity co-drive the effect of drought on litter decomposition.This experimental finding provides a new insight into the mechanisms controlling forest floor C and nutrient cycling under future global change scenarios.

Mobility Analysis of the Deployable Structure of SLE Based on Screw Theory

Scissor-like element has a number of applications in deployable structures such as planar deployable structure (PDS) and ring deployable structure(RDS). However, the mobility analysis of the multi-loop deployable structures is made more difficulty by the traditional mobility formula, because the deployable structure is a very complex structure with multi-loop. Therefore, On the basis of screw theory, the calculation method of mobility of deployable structures of SLE is thoroughly discussed. In order to investigate the mobility, decomposing and composing structures(DCS) are developed, and the basic units are able to be obtained. On the basis of the deployable structures’ geometrical characteristics, there exists a closed-loop quadrilateral structure and some non-closed-loop quadrilateral structures in PDS. Also, a six legs parallel structure is present in RDS. The basic units’ mobility can be solved by both the methods of screw theory and topology constraint graphs. Then, composing the related basic units, the formula of planar deployable structures’ mobility can be built and solves the mobility of ring deployable structure. The analysis method solves the mobility analysis of the multi-loop deployable structures which is difficulty by the traditional method, and plays an important role in further research about the mobility of other complex deployable structures.

一种基于优化阈值函数的整体强化分解模型改进降噪方法

以提高降噪效果为目的,提出了一种基于白噪声分解特性的EEMD优化阈值降噪方法。避免了小波分解时选择合适小波基函数的困难,具有自适应性,同时,也可以有效地避免频率混叠问题。方法从能量密度和对应平均周期乘积的变化率出发,提出了"跳变点"和"奇异点"的选择原则,并利用优化阈值函数对"跳变点"和"奇异点"对应的IMF分量进行量化处理,然后通过对处理后的IMF分量重构得到降噪信号。最后用仿真信号进行试验,证明本方法的有效性和优越性。

Research Progress on Application of Rape as Green Manure

Rape is a crop that can be widely used as a green manure.After returning to the field,it has the ability to fertilize and improve the quality of agricultural products.This paper summarized the application model of rape as a green manure and the decomposing characteristics of rape after being returned to the field,and clarified the ecological effects of returning rape to the field,with an attempt to provide scientific basis for the reasonable use of rape and nutrient management in farmland.

A semi-custom design methodology for design performance optimization

We present a semi-custom design methodology based on transistor tuning to optimize the design performance. Compared with other transistor tuning approaches, our tuning process takes the cross-talk effect into account and prominently reduces the complexity for circuit simulation and analysis by decomposing the circuit network utilizing graph theory. Furthermore, the incremental placement and routing for the corresponding transistor tuning in conventional approaches is not required in our methodology, which might induce timing graph variation and additional iterations for design convergence. This methodology combines the flexible automated circuit tuning and physical design tools to provide more opportunities for design optimization throughout the design cycle.