Using hybridoma technique, we prepared the monoclonal antibody against a1-AT and combined it with Immuno-Chemical Monitor System-(ICS)-rate nephelemetry to determine the serum a1-AT concentration of 50 health adults, ...Using hybridoma technique, we prepared the monoclonal antibody against a1-AT and combined it with Immuno-Chemical Monitor System-(ICS)-rate nephelemetry to determine the serum a1-AT concentration of 50 health adults, 49 patients with primary hepatic carcinoma (PHC) and 52 with benign liver diseases, respectively. Serum a1-AT levels were significantly higher in patients with PHC than in normal adults (P<0.001). Elevated levels of a1-AT were found in 43% of patients with PHC. No difference was found in a1-AT between patients with benigh liver diseases and health adults (P>0.05). The results indicated that a1-AT is one of the serum markers useful for diagnosing PHC. It is hopeful by using the monoclonal antibody against a1-AT as a new reagent to examine a1-AT on the molocular cytological level.展开更多
The incidence and mortality rates of gastrointestinal(GI)cancer remain high.Despite constant improvements in diagnostic and therapeutic techniques,the early diagnosis,mid-and late-stage treatment,drug tolerance,and ca...The incidence and mortality rates of gastrointestinal(GI)cancer remain high.Despite constant improvements in diagnostic and therapeutic techniques,the early diagnosis,mid-and late-stage treatment,drug tolerance,and cancer recurrence and metastasis in GI cancer remain challenging.In this review article we summarize the recent research advance in the roles of keratins in GI cancer,with the hope that they will become efficient biomarkers for the prediction,diagnosis,or treatment of these malignancies.展开更多
Objective:The clinical features and test results of 2019-nCoV pneumonia(COVID-19)were analyzed retrospectively in order to understand the diagnostic significance of clinical test index,and to summarize the experience ...Objective:The clinical features and test results of 2019-nCoV pneumonia(COVID-19)were analyzed retrospectively in order to understand the diagnostic significance of clinical test index,and to summarize the experience of clinical treatment.Methods:From February 10thto February 28th,2020,54 patients with COVID-19 from Wuhan Red Cross hospital were included,whose clinical data were analyzed and integrated.The clinical manifestation and laboratory examination were analyzed by descriptive statistical analysis.Results:The average age of the patients was 63.9 years(40–84 years).The median time from onset to diagnosis was 6.5 days(2–20 days),and the median time from onset to first hospitalization was 8 days(3–20 days).The main manifestations were that the percentage of neutrophil increased and the number of lymphocyte decreased in most cases.Most of them were combined with liver damage and myocardial cell damage,and chest CT showed lesion of bilateral lung.Conclusion:In addition to the nucleic acid test of 2019-nCoV,some laboratory tests may indicate COVID-19 in the early stages.The clinician may conduct repeated follow-up examinations to assess the progress and outcome of the disease.展开更多
文摘Using hybridoma technique, we prepared the monoclonal antibody against a1-AT and combined it with Immuno-Chemical Monitor System-(ICS)-rate nephelemetry to determine the serum a1-AT concentration of 50 health adults, 49 patients with primary hepatic carcinoma (PHC) and 52 with benign liver diseases, respectively. Serum a1-AT levels were significantly higher in patients with PHC than in normal adults (P<0.001). Elevated levels of a1-AT were found in 43% of patients with PHC. No difference was found in a1-AT between patients with benigh liver diseases and health adults (P>0.05). The results indicated that a1-AT is one of the serum markers useful for diagnosing PHC. It is hopeful by using the monoclonal antibody against a1-AT as a new reagent to examine a1-AT on the molocular cytological level.
基金supported by the Wu Jieping Medical Foundation(320.6750.19020).
文摘The incidence and mortality rates of gastrointestinal(GI)cancer remain high.Despite constant improvements in diagnostic and therapeutic techniques,the early diagnosis,mid-and late-stage treatment,drug tolerance,and cancer recurrence and metastasis in GI cancer remain challenging.In this review article we summarize the recent research advance in the roles of keratins in GI cancer,with the hope that they will become efficient biomarkers for the prediction,diagnosis,or treatment of these malignancies.
文摘Objective:The clinical features and test results of 2019-nCoV pneumonia(COVID-19)were analyzed retrospectively in order to understand the diagnostic significance of clinical test index,and to summarize the experience of clinical treatment.Methods:From February 10thto February 28th,2020,54 patients with COVID-19 from Wuhan Red Cross hospital were included,whose clinical data were analyzed and integrated.The clinical manifestation and laboratory examination were analyzed by descriptive statistical analysis.Results:The average age of the patients was 63.9 years(40–84 years).The median time from onset to diagnosis was 6.5 days(2–20 days),and the median time from onset to first hospitalization was 8 days(3–20 days).The main manifestations were that the percentage of neutrophil increased and the number of lymphocyte decreased in most cases.Most of them were combined with liver damage and myocardial cell damage,and chest CT showed lesion of bilateral lung.Conclusion:In addition to the nucleic acid test of 2019-nCoV,some laboratory tests may indicate COVID-19 in the early stages.The clinician may conduct repeated follow-up examinations to assess the progress and outcome of the disease.
