豆科与木兰科鸡血藤类药材活性成分及药理作用研究进展

鸡血藤是传统的活血化瘀类中药,具有活血补血、调经止痛、舒筋活络的功效,临床应用广泛。约有26种药用植物曾作为鸡血藤在临床使用,其所含化学成分复杂多样,主要包括黄酮、蒽醌、木脂素、三萜及挥发油等,表现出促进造血功能、抗肿瘤、抗炎、抗氧化、保肝及抗人免疫缺陷病毒(HIV)等多种药理活性。根据本草著作、文献研究和现代应用情况,分别对来自豆科和木兰科的11种鸡血藤类药材的主要化学成分和药理活性进行综述,对比两者所含成分及药理活性的异同,为其进一步的开发与合理利用提供依据。

Genome sizes of four important medicinal species in Kadsura by flow cytometry

Objective:Dianjixueteng is a geoherb in Yunnan Province,the source plant of which is Kadsura interior.However,the formation of this geoherb is not clear in genetic mechanism,in which genome size is the first step that should be known on the genomic level.In this study we aimed to estimate the genome sizes of source plants of K.interior and three related herbs K.heteroclita,K.longipedunculata,and K.coccinea by flow cytometry(FCM)and make a comparison.Methods:The genome sizes of K.interior,K.heteroclita,K.longipedunculata and K.coccinea,i.e.,the source plants of Dianjixueteng and its relative medicinal materials,were estimated by FCM.The nuclei of K.interior were isolated using modified LB01 buffer,for the rest species,by the Galbraith’s buffer.Results:The genome sizes of K.interior,K.heteroclita,K.longipedunculata,and K.coccinea were 7.36,7.12,7.01,and 5.15 pg/1 C,respectively.Genome size of K.interior had no significant variation with those of K.heteroclita and K.longipedunculata(P=0.296),which was significantly larger than that of K.coccinea.Conclusion:Genome size can not distinguish K.interior from K.heteroclita and K.longipedunculata,but could distinguish them from K.coccinea,which lays the foundation for future studies on genetic mechanism of the geoherb formation.

复方滇鸡血藤颗粒的指纹图谱建立及4个木脂素类成分含量测定

目的:建立复方滇鸡血藤颗粒的指纹图谱,并建立颗粒中4个木脂素类成分同时测定的高效液相色谱(HPLC)方法,为其质量控制提供参考。方法:采用HPLC法建立复方滇鸡血藤颗粒的指纹图谱,以对照品进行共有峰指认,通过制剂与各单味药材的相关性分析进行共有峰归属,通过相似度评价结合化学模式识别分析11批样品的质量差异,同时以4个木脂素类成分戈米辛D、内南五味子素、异型南五味子丁素、南五味子素为指标性成分,建立其含量测定方法。结果:建立了复方滇鸡血藤颗粒的11批样品指纹图谱,共标定20个共有峰,其中1号峰为马钱苷、3号峰为羟基红花黄色素A、4号峰为杯苋甾酮、6号峰为大豆苷元、13号峰为戈米辛D、17号峰为内南五味子素、19号峰为异型南五味子丁素、20号峰为南五味子素;各批次样品间相似度均大于0.97,可聚为2类,4个主成分的累积贡献率达89.764%;4个木脂素类成分戈米辛D、内南五味子素、异型南五味子丁素、南五味子素的含量分别为386.35~426.96、280.69~307.66、693.31~740.91、188.66~215.59μg·g^(-1)。结论:所建立的指纹图谱及含量测定方法可为复方滇鸡血藤颗粒的质量控制与评价提供依据。