Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods...Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods Primary cultured hippocampal neurons, the cultures were pretreated with tSDA and GRb1 on 10d for 24 hours respectively. Then the cultures were treated with 35 μmol·L -1 Aβ25-35 for 24 hours, observed the changing of survival rate of neurons and the apoptosis of neurons with biochemical analysis combining immunofluorescent cytochemical double-staining technique. Results Hippocampal neurons were treated with 35 μmol·L -1 Aβ for 24 hours, and survival rate of neurons downed to 52.6%. When neurons were pretreated by tSDA and GRb1, survival rate of neurons increased 11% to 15%. The findings of immunofluorescent cytochemical double-staining indicated that apoptotic neurons were obviously more than that of the blank group, reaching 43.9%.When neurons were pretreated by tSDA and GRb1, apoptotic neurons were downed to 16.6%, 10.8% respectively. Conclusion tSDA had the same effects as GRb1, protecting the neurons, antagonizing neurotoxicity of Aβ, increasing survival rate of neurons, and reducing apoptotic neurons induced by Aβ.展开更多
Objective To study the effects of Dipsacus Asper and Vitamin E on the SS neurons in the hippocampal formation of rat models of Alzheimer’s Disease (AD). Methods Established rat models of AD by giving water containing...Objective To study the effects of Dipsacus Asper and Vitamin E on the SS neurons in the hippocampal formation of rat models of Alzheimer’s Disease (AD). Methods Established rat models of AD by giving water containing AlCl 3, then treating them with Dipsacus Asper and Vitamin E(VE) for three months, observed the changing condition of rats’ memory through behavior tests, and studied changes of SS neurons in hippocampal formation with immunohistochemical ABC method. Results 3 months after treatment, behavior tests showed that rats’ memory was improved and the SS neurons in each region of hippocampal formation were increased, In CA1,CA2,CA3 and dentate gyrus, there were significant differences among treated groups and control group( P <0.05). In addition to the differences of quantity, the shape of SS neurons changed too: cytoplasm was stained strongly and equally, bodies and processes were rather clear.Conclusion Dipsacus Asper and Vitamin E can restore the SS neurons in AD models and SS neurons in hippocampal formation are related to AD’s cause and development.展开更多
Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begi...Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begins growth earlier in spring than its native competitors. The purpose of this study was to find a time both seasonally and in cutleaf teasel’s life history when herbicides could be applied to decrease teasel with the least impact on the surrounding vegetation. We tested the effects of three different herbicides (glyphosate (Round-UpTM), triclopyr amine (GarlonTM), and clopyralid (LontrelTM)) on cut and uncut teasel at three different times of the year (July and October 2005, and April 2006) near Clinton Lake in Dewitt Co. Illinois. Photosynthetic measurements were taken before application to determine teasel’s susceptibility to the herbicides, and we harvested seed heads and rosettes in late October 2006. Results indicated teasel was photosynthetically active at all three application times. Cutting before herbicide application had no significant effect on the number of seeds produced or the dry weight of the rosettes. Herbicide treatment in April significantly reduced the amount of seeds produced, but there were no significant differences among the three herbicides. Clopyralid application in April significantly reduced rosette biomass, but none of the herbicides significantly affected rosette biomass at the other two times. Our studies suggest herbicide application early in the growing season may be beneficial in controlling the spread of teasel, and that mowing at the time of spraying will not increase effectiveness of the herbicide.展开更多
AIM: To establish and apply a new LC/MS/MS method for the simultaneous, quantitative determination of six ingredients, aucubin(AU), geniposide(GP), geniposidic acid(GPA), pinoresinol diglucoside(PDG), secologanin(SLG)...AIM: To establish and apply a new LC/MS/MS method for the simultaneous, quantitative determination of six ingredients, aucubin(AU), geniposide(GP), geniposidic acid(GPA), pinoresinol diglucoside(PDG), secologanin(SLG), and loganin(LG) in single and combined extracts of Eucommia ulmoides and Dipsacus asperoides. METHOD: Using the LC/MS/MS-ESI--MRM mode to detect the six compounds, chromatographic separation was achieved on an Agilent Eclipse plus C18 column, and the mobile phase consisted of solvent A(CH3CN) and solvent B(H2O containing 0.01% CH3 COOH V/V). RESULTS: This method was successfully applied to quantify the six compounds in rat plasma after oral administration, and showed good precision, accuracy, reproducibility, and linear regression(r2 >0.99). CONCLUSION: The results showed that following the use of the two medicinal plants, for AU and GP, the values of tmax markedly increased, and the values of cmax markedly decreased. It was found that the compatibility of the medicinal plants might affect their pharmacokinetic properties of their constituents.展开更多
文摘Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods Primary cultured hippocampal neurons, the cultures were pretreated with tSDA and GRb1 on 10d for 24 hours respectively. Then the cultures were treated with 35 μmol·L -1 Aβ25-35 for 24 hours, observed the changing of survival rate of neurons and the apoptosis of neurons with biochemical analysis combining immunofluorescent cytochemical double-staining technique. Results Hippocampal neurons were treated with 35 μmol·L -1 Aβ for 24 hours, and survival rate of neurons downed to 52.6%. When neurons were pretreated by tSDA and GRb1, survival rate of neurons increased 11% to 15%. The findings of immunofluorescent cytochemical double-staining indicated that apoptotic neurons were obviously more than that of the blank group, reaching 43.9%.When neurons were pretreated by tSDA and GRb1, apoptotic neurons were downed to 16.6%, 10.8% respectively. Conclusion tSDA had the same effects as GRb1, protecting the neurons, antagonizing neurotoxicity of Aβ, increasing survival rate of neurons, and reducing apoptotic neurons induced by Aβ.
文摘Objective To study the effects of Dipsacus Asper and Vitamin E on the SS neurons in the hippocampal formation of rat models of Alzheimer’s Disease (AD). Methods Established rat models of AD by giving water containing AlCl 3, then treating them with Dipsacus Asper and Vitamin E(VE) for three months, observed the changing condition of rats’ memory through behavior tests, and studied changes of SS neurons in hippocampal formation with immunohistochemical ABC method. Results 3 months after treatment, behavior tests showed that rats’ memory was improved and the SS neurons in each region of hippocampal formation were increased, In CA1,CA2,CA3 and dentate gyrus, there were significant differences among treated groups and control group( P <0.05). In addition to the differences of quantity, the shape of SS neurons changed too: cytoplasm was stained strongly and equally, bodies and processes were rather clear.Conclusion Dipsacus Asper and Vitamin E can restore the SS neurons in AD models and SS neurons in hippocampal formation are related to AD’s cause and development.
文摘Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begins growth earlier in spring than its native competitors. The purpose of this study was to find a time both seasonally and in cutleaf teasel’s life history when herbicides could be applied to decrease teasel with the least impact on the surrounding vegetation. We tested the effects of three different herbicides (glyphosate (Round-UpTM), triclopyr amine (GarlonTM), and clopyralid (LontrelTM)) on cut and uncut teasel at three different times of the year (July and October 2005, and April 2006) near Clinton Lake in Dewitt Co. Illinois. Photosynthetic measurements were taken before application to determine teasel’s susceptibility to the herbicides, and we harvested seed heads and rosettes in late October 2006. Results indicated teasel was photosynthetically active at all three application times. Cutting before herbicide application had no significant effect on the number of seeds produced or the dry weight of the rosettes. Herbicide treatment in April significantly reduced the amount of seeds produced, but there were no significant differences among the three herbicides. Clopyralid application in April significantly reduced rosette biomass, but none of the herbicides significantly affected rosette biomass at the other two times. Our studies suggest herbicide application early in the growing season may be beneficial in controlling the spread of teasel, and that mowing at the time of spraying will not increase effectiveness of the herbicide.
基金supported by the Program for Important Drug Development of MOST,China(2012ZX 09304007)the Important Drug Development of MOST,China(2013ZX09401-004-002)+1 种基金the Tianjin Committee of Science and Technology,China(10SYSYJC28900)Natural Science Foundation of China(NSFC81125024)
文摘AIM: To establish and apply a new LC/MS/MS method for the simultaneous, quantitative determination of six ingredients, aucubin(AU), geniposide(GP), geniposidic acid(GPA), pinoresinol diglucoside(PDG), secologanin(SLG), and loganin(LG) in single and combined extracts of Eucommia ulmoides and Dipsacus asperoides. METHOD: Using the LC/MS/MS-ESI--MRM mode to detect the six compounds, chromatographic separation was achieved on an Agilent Eclipse plus C18 column, and the mobile phase consisted of solvent A(CH3CN) and solvent B(H2O containing 0.01% CH3 COOH V/V). RESULTS: This method was successfully applied to quantify the six compounds in rat plasma after oral administration, and showed good precision, accuracy, reproducibility, and linear regression(r2 >0.99). CONCLUSION: The results showed that following the use of the two medicinal plants, for AU and GP, the values of tmax markedly increased, and the values of cmax markedly decreased. It was found that the compatibility of the medicinal plants might affect their pharmacokinetic properties of their constituents.
