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The "Silk Road Economic Belt" and the "China Dream" Relationship: A Strategy or Tactic
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作者 Xing Li Wan Wang 《Sociology Study》 2015年第3期169-175,共7页
The "Silk Road Economic Belt" and the "China Dream" are tightly linked together rather than isolated from each other. The essence of the construction of the "Silk Road Economic Belt" is to give equal considerati... The "Silk Road Economic Belt" and the "China Dream" are tightly linked together rather than isolated from each other. The essence of the construction of the "Silk Road Economic Belt" is to give equal considerations to both the East and the West, paying equal attentions to the North and the South, while balancing the land and the sea and connecting domestic with foreign. This is the innovation and priority of China's peripheral diplomacy as well as a strategic support and tactical guarantee of the "China Dream". The "Silk Road Economic Belt" is constructed to make history serve reality; it exchanges economy for politics, space on land for time at sea, and balances a weakness in sea power with superiority in land power. Instead of simply replicating the Silk Road, the "Silk Road Economic Belt" possesses a broader realm and a richer context. Moreover, this is China's ultimate strategy and profound tactical conception facing toward the future. 展开更多
关键词 "Silk Road Economic Belt" "China dream" the center of Eurasia STRATEGY tactic
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Study on "China Dream" Educational Propaganda Integrating into Education of Ideological and Political Education
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作者 Tian XlA 《International English Education Research》 2014年第11期22-24,共3页
"China Dream", not only is the national dream, but also is the dream of all the Chinese people, and highly fits the educational purpose of university education, "China Dream" leads the university ideal faith educa... "China Dream", not only is the national dream, but also is the dream of all the Chinese people, and highly fits the educational purpose of university education, "China Dream" leads the university ideal faith education to a higher standard, and embodied in the "China Dream", and enriched the connotation of the socialist core value system, is the organic unity of national spirit and the spirit of the age; "China Dream" represents a blue view for the great rejuvenation of the Chinese nation, is the organic unity of ideal and great practice being together; Also, the ideological and political education had also established in the underdevelopment contemporary youth growing up; the value of coordination shaped college student's right of personal value and social value. "China Dream", was the sublimation of university ideological and political education, ever advancing with the time, the "China Dream" tightly integrated into the university ideological and political education, strives to cultivate the endeavor of having both ability and political integrity, and having the national feelings of dedication. Therefore, college ideological and political education work is to "China Dream" as the guider, actively promotes the educational model innovation. 展开更多
关键词 "China dream" University Ideological and Political Education Propaganda.
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No Dream, No Future: A Study on Aesthetic Dimensions of"Chinese Dream" and the Task of Literary Critics
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作者 WANG Jie 《Journal of Literature and Art Studies》 2015年第12期1107-1114,共8页
This article believes that "Chinese Dream" has three dimensions: First, "Chinese Dream" is the inevitable demands of Chinese reconstruction and it is the deepest desire of Chinese nation since the Chinese modern ... This article believes that "Chinese Dream" has three dimensions: First, "Chinese Dream" is the inevitable demands of Chinese reconstruction and it is the deepest desire of Chinese nation since the Chinese modern history. Second, the modernism of socialism with Chinese feature is the process of Chinese modernism. Third, the third theoretical dimension, 1 believe, is the ideal of socialism. I would like to take the film "Lost" and "Peking Monk" as examples and analyze the specific form of expression. This article is going to indicate in the end that "the revolution of aesthetics" and the assignments of critics, which can be recognized as the intuitive ability, could merge the historical affair and the human nature, which literature comprise, together and deliver deeper analyses and appraise The mission of critics is not only to distinctly emerge the aesthetic form of the literal works, but also analyze and seize the "aesthetic form" of them, that is to say, their mission is to find the aesthetic meanings of aesthetic expression from commonage in order to achieve the goal of leading and stimulating people ensuing the consciousness and liberation of culture. That is the most important mission and responsibility of Marxist literal criticism and literal critics. This is a rather tough task and refers to the new understanding and attitude to the relative questions of literature and culture. In the eyes of some self-complacent intellectuals, the responsibility for literature and critics is the "aesthetic entertainment" and "spiritual redemption". The significance of Marxist Aesthetics lies in that it upgrades the general "literal principle" and "aesthetic principle" in to a level of social development and national deliverance, and also it regards whether the literature having the function of cultural libration as the more pivotal content. The assignment for the critics and litterateurs is to engage and promote the production and promulgate for this significance, the cultural consciousness and liberation. 展开更多
关键词 Chinese dream aesthetic dimensions the revolution of aesthetics cultural libration
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Research fit under the "China Dream" background Chinese Wushu industry and traditional culture
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作者 Wang Wen 《Review of Global Academics》 2014年第2期140-146,共7页
In this paper, the author uses literature data and logical analysis in the ',China Dream" background and discusses the opportunities and challenges of Chinese Wushu industry. The author points out that China's deve... In this paper, the author uses literature data and logical analysis in the ',China Dream" background and discusses the opportunities and challenges of Chinese Wushu industry. The author points out that China's development should be directed to the development of the fusion of traditional Chinese culture, to help China establish an energetic, self-improvement image and the pursuit of harmony and common prosperity. The image of peaceful rise of a big country enhances China's international status and influence Martial Arts and Martial industrial development should focus on the rich cultural connotations, tap the cultural symbol it could develop, build martial arts industry brands through various media channels, government support and other ways to expand the widest range of consumer groups, and expand development of martial arts industry 展开更多
关键词 Chinese dream Martial arts industry Chinese traditional culture
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Construction the Value Dimensions of"Chinese Dream" Take the Film American Dreams in China as an Example
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作者 YIN Qing-hong 《Journal of Literature and Art Studies》 2015年第12期1123-1128,共6页
In recent years, the "China Dream" has become a popular topic in China's Mainland. To tell the stories of the "Chinese Dream" from different aspect in the field of literary and artistic creation have emerged a... In recent years, the "China Dream" has become a popular topic in China's Mainland. To tell the stories of the "Chinese Dream" from different aspect in the field of literary and artistic creation have emerged as a significant phenomenon. To some extent, these texts can be regarded as national allegory. This paper will take the film American Dreams in China as a case, to provide some thoughts on the value dimensions for the construction of the "Chinese Dreams". 展开更多
关键词 Chinese dream National Allegory Value Dimensions
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基于DREAM算法的DSGE模型参数估计精度研究
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作者 罗琦 赵胜民 《统计与决策》 CSSCI 北大核心 2024年第2期46-51,共6页
文章将人工智能算法中的DREAM算法首次应用到动态随机一般均衡模型的参数估计中,并以动态随机一般均衡模型LS(2005)为例对该算法的估计精度进行了系统分析,研究结果表明:根据待估参数随机抽样序列的箱线图来看,由DREAM算法产生的待估参... 文章将人工智能算法中的DREAM算法首次应用到动态随机一般均衡模型的参数估计中,并以动态随机一般均衡模型LS(2005)为例对该算法的估计精度进行了系统分析,研究结果表明:根据待估参数随机抽样序列的箱线图来看,由DREAM算法产生的待估参数随机抽样序列的箱体长度均比RWMH和IMH算法产生的箱体长度要长,说明由DREAM算法产生的参数估计序列的分散程度比RWMH和IMH算法要大,表明了DREAM算法遍历参数空间范围更为广泛,算法逃逸局部最优值的能力更强。另外,从箱线图中的中位数数值来看,除了5个参数以外,由DREAM算法产生的参数估计序列的中位数相比RWMH和IMH算法,与真实数据生成过程更为接近,说明由DREAM算法产生的参数估计值大部分都集中在参数的真值附近。由于DREAM算法依据IQR统计方法除去无用链,故由DREAM算法产生的参数估计序列的异常值也明显降低,而RWMH算法产生的参数估计序列的异常值尤其多。从待估参数的90%最大后验密度可信区间来看,DREAM算法产生的待估参数90%最大后验密度可信区间除了3个参数以外,其余全部包含了真值,而传统的RWMH和IMH算法分别只有7个和1个区间包含了真值,表明DREAM算法的估计不确定性远小于传统的RWMH和IMH算法。最后,根据待估参数的无效因子来看,DREAM算法产生的待估参数序列与传统的RWMH和IMH算法相比,其相关性更弱,即无效因子数值更小,这一点进一步验证了DREAM算法遍历整个参数空间的能力更强。 展开更多
关键词 dream算法 DSGE模型 估计精度
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Dreaming across Languages and Cultures
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作者 ZHOU LIN 《China Today》 2024年第11期64-66,共3页
From a Ph.D.student to a budding sinologist,a young Spanish woman explains her vision for more cultural exchanges between China and her country.DREAM of the Red Chamber,Cao Xueqin’s classic about the rise and decline... From a Ph.D.student to a budding sinologist,a young Spanish woman explains her vision for more cultural exchanges between China and her country.DREAM of the Red Chamber,Cao Xueqin’s classic about the rise and decline of a family that is also a portrayal of its contemporary Qing Dynasty(1644-1911),continues to inspire and influence long after it was published in the 18th century.Patricia Amate Núñez is a current acolyte. 展开更多
关键词 dream CONTINUE EXCHANGES
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The Application of Embodied Cognitive Linguistics in the English Translation of Two-Part Allegorical Saying—An Analysis of Examples From A Dream of Red Mansions
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作者 HE Jiamei ZOU Jianling 《Sino-US English Teaching》 2024年第7期337-342,共6页
As a kind of widely circulated folk language,two-part allegorical saying(“xiehouyu”)carries the wisdom of the Chinese people and is characterized with humor and vivid imagery.Taking the two English translation versi... As a kind of widely circulated folk language,two-part allegorical saying(“xiehouyu”)carries the wisdom of the Chinese people and is characterized with humor and vivid imagery.Taking the two English translation versions of A Dream of Red Mansions as examples,this paper analyzes the English translation of two-part allegorical saying based on the core principle of Embodied Cognitive Linguistics(ECL),“Reality-Cognition-Language”,and explores how the English translation of two-part allegorical saying reflects the three dimensions of ECL. 展开更多
关键词 two-part allegorical saying A dream in Red Mansions Embodied Cognitive Linguistics TRANSLATION
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Translation Studies from a Cross-Cultural Perspective:Reinventing Subjectivity-A Case Study of English Translation of A Dream of Red Mansions
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作者 YANG Yin-chuan YANG Zhan 《Journal of Literature and Art Studies》 2024年第4期279-283,共5页
The integration of cross-cultural inquiries into the realm of translation has given rise to a distinct subfield known as cross-cultural translation studies.This approach emphasizes the importance of delving into the c... The integration of cross-cultural inquiries into the realm of translation has given rise to a distinct subfield known as cross-cultural translation studies.This approach emphasizes the importance of delving into the cultural subtleties and the awareness of cultural subjectivity within the translation process.Central to this framework is the concept of reshaping cultural nuances and the subjectivity of culture.This paper introduces three principal translation strategies that emerge from a cross-cultural standpoint:the alienation translation strategy,the‘cannibalism’translation strategy,and the destructive translation strategy.Utilizing the English translation of“Dream of the Red Chamber”as a case study,the paper examines how cultural nuances and cultural subjectivity influence the selection of translation strategies and demonstrates the practical application of cross-cultural research within the translation sphere.Hence,the significance of cross-cultural translation studies lies in its capacity to offer a novel lens through which to view translation studies.It encourages a reevaluation of cultural nuances and subjectivity in translation,which is of profound practical importance for advancing the field of translation studies,enhancing cross-cultural communication,and facilitating the spread of Chinese discourse on the global stage. 展开更多
关键词 CROSS-CULTURAL translation strategies A dream of Red Mansions
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Living the Mechanical Dream
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作者 KEDIR ABDUREZAK NURIYE 《ChinAfrica》 2024年第3期56-56,共1页
Studying in China is not just about academics,it is also about connecting with communities and learning local culture.As a child,I was captivated by machines and their inner workings.I yearned to design contraptions t... Studying in China is not just about academics,it is also about connecting with communities and learning local culture.As a child,I was captivated by machines and their inner workings.I yearned to design contraptions that could solve real-world problems and improve people’s lives.So,when it came time to choose a university,I knew that mechanical engineering was the path for me. 展开更多
关键词 earned connecting dream
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Fascination For Planes Young Ghanaian pursues her aviation dream in China
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作者 GE LIJUN 《ChinAfrica》 2024年第5期54-55,共2页
Every morning at 7 o’clock,Rachael Amoah,a young woman from Ghana,prepares for a new day of challenges and innovations at a leading aerospace company in Shanghai’s Pudong New Area.In March last year,she completed he... Every morning at 7 o’clock,Rachael Amoah,a young woman from Ghana,prepares for a new day of challenges and innovations at a leading aerospace company in Shanghai’s Pudong New Area.In March last year,she completed her studies in the School of Aeronautics and Astronautics at Shanghai Jiao Tong University(SJTU),and fulfilled her ambition to work in this megalopolis. 展开更多
关键词 AVIATION dream pursue
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DREAM算法分析地下水数值模拟不确定性的影响因素 被引量:6
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作者 杨运 吴吉春 +1 位作者 骆乾坤 吴剑锋 《地质论评》 CAS CSCD 北大核心 2016年第2期353-361,共9页
地下水数值模拟常受到模型不确定性、观测资料不确定性等多种不确定性因素的影响,对这些影响因素进行定量分析十分必要。将差分进化自适应梅特罗波利斯(Differential Evolution Adaptive Metropolis,DREAM)算法与MODFLOW模型结合应用于... 地下水数值模拟常受到模型不确定性、观测资料不确定性等多种不确定性因素的影响,对这些影响因素进行定量分析十分必要。将差分进化自适应梅特罗波利斯(Differential Evolution Adaptive Metropolis,DREAM)算法与MODFLOW模型结合应用于地下水数值模拟不确定性的定量分析。以模型结构概化、水位观测资料误差这两种重要不确定性来源为例,通过一个理想地下水流模型,系统分析两者对模型参数及模型输出结果不确定性的影响。研究结果表明:模型结构概化及水位观测资料误差共同引起了地下水数值模拟的不确定性,但模型结构概化起到了主控作用。模型结构概化合理时,模型参数及模型输出结果的不确定性较小,并随观测资料误差不确定性的增大而增大;模型结构概化不合理时,模型参数及模型输出结果主要受控于模型结构概化带来的影响,且不确定性显著增大;观测资料误差相同情况下,模型结构概化越接近于真实的水文地质条件,模型参数及模型输出结果的不确定性越小。 展开更多
关键词 dream 模型结构概化 观测资料误差 数值模拟 不确定性 地下水
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基于Access和Dream Weaver软件的实验室菌种管理系统设计与应用 被引量:4
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作者 刘畅 黄文睿 +6 位作者 汪永喆 叶萌 方泰惠 刘景晶 罗新龙 范晓旭 曹荣月 《实验技术与管理》 CAS 北大核心 2011年第1期100-103,共4页
利用开放性实验和实践创新训练的教学模式为学生提供实践机会,指导学生参与实验室中的基因工程菌菌种管理。针对实验室构建的工程菌的品种多、数量大、流动快等特点,利用Microsoft Office Access2003和Macromedia Dream Weaver 8.0设计... 利用开放性实验和实践创新训练的教学模式为学生提供实践机会,指导学生参与实验室中的基因工程菌菌种管理。针对实验室构建的工程菌的品种多、数量大、流动快等特点,利用Microsoft Office Access2003和Macromedia Dream Weaver 8.0设计了一种简单易行的菌种管理系统,对现有菌种进行良好的统计和查找。阐述了如何使用Microsoft Office Access 2003和Macromedia Dream Weaver 8.0软件管理实验室菌种,并介绍了该系统的实际应用。 展开更多
关键词 基因工程菌 ACCESS dream Weaver 实验室管理 开放性实验
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染料木素磺酸钠对脑缺血再灌注损伤的保护作用及DREAM和PSD95的表达变化 被引量:8
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作者 钟星明 黄志华 +3 位作者 龚衍 黎晓 李良东 曾靖 《中风与神经疾病杂志》 CAS CSCD 北大核心 2013年第8期697-699,共3页
目的研究染料木素磺酸钠(GSS)对脑缺血再灌注损伤的保护作用,并初步探讨转录抑制子DREAM及突触后致密区蛋白95(PSD95)表达的变化。方法以大鼠大脑中动脉栓塞制作局灶性脑缺血再灌注损伤模型,缺血后10min于舌下静脉给不同剂量的GSS。再灌... 目的研究染料木素磺酸钠(GSS)对脑缺血再灌注损伤的保护作用,并初步探讨转录抑制子DREAM及突触后致密区蛋白95(PSD95)表达的变化。方法以大鼠大脑中动脉栓塞制作局灶性脑缺血再灌注损伤模型,缺血后10min于舌下静脉给不同剂量的GSS。再灌注24h,行神经功能学评分。麻醉后,腹腔静脉取血并分离血清。取脑,一组前脑做TTC染色,测定脑梗死体积;另一组取损伤侧脑组织制成10%的脑组织匀浆,比色法测血清及脑组织匀浆LDH活性;另取半暗带区脑组织,Western blot方法检测脑组织DREAM蛋白的表达。结果模型组大鼠出现明显的脑梗死区及神经功能缺损,血清LDH活性升高(P<0.01),缺血侧脑组织LDH活性降低(P<0.05),皮层半暗带区脑组织DREAM蛋白表达降低(P<0.05),PSD95蛋白表达升高(P<0.001)。与模型组相比,1mg/kg及2mg/kg GSS治疗组脑梗死体积减小;神经功能评分降低(P<0.01,P<0.05),血清LDH活性降低(P<0.05),缺血侧脑组织LDH活性升高(P<0.05);2mg/kg GSS治疗组皮层半暗带区DREAM蛋白表达升高(P<0.01),PSD95蛋白表达降低(P<0.05)。结论 GSS对脑缺血再灌注损伤具有保护作用,其保护机制与GSS提高转录抑制子DREAM的表达,从而抑制NMDA受体介导的兴奋性毒性损伤有关。 展开更多
关键词 染料木素磺酸钠 脑缺血再灌注损伤 dream
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基于DREAM算法的含水层渗透系数空间变异特征识别 被引量:4
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作者 骆乾坤 吴剑锋 +2 位作者 杨运 吴吉春 马淑芬 《南京大学学报(自然科学版)》 CAS CSCD 北大核心 2016年第3期448-455,共8页
采用差分进化自适应Metropolis(DREAM)算法对描述含水层渗透系数空间变异特征的参数进行识别.利用直接傅里叶变换方法产生一组空间结构参数下的若干个渗透系数场实现,借鉴噪声遗传算法(NGA)思想,计算该组空间结构参数对应的贝叶斯后验... 采用差分进化自适应Metropolis(DREAM)算法对描述含水层渗透系数空间变异特征的参数进行识别.利用直接傅里叶变换方法产生一组空间结构参数下的若干个渗透系数场实现,借鉴噪声遗传算法(NGA)思想,计算该组空间结构参数对应的贝叶斯后验概率值,提高DREAM算法求解的效率.算例求解结果表明,DREAM算法能够有效获得含水层渗透系数空间结构参数的后验分布,并可得到对应的一系列渗透系数场,可为含水层参数空间变异性研究提供新的思路. 展开更多
关键词 渗透系数 空间变异特征 噪声遗传算法 dream
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DREAM基因小分子干扰RNA重组腺相关病毒载体的构建与鉴定 被引量:4
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作者 陈敏 项红兵 田玉科 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2011年第1期75-79,共5页
目的构建表达DREAM基因的小分子干扰RNA(siRNA)重组腺相关病毒(rAAV)载体,并观察感染PC12细胞后DREAM蛋白表达受抑制情况。方法设计并合成shRNA对应的2条互补的寡核苷酸链,pDC316-EGFP-U6质粒经BamHⅠ和HindⅢ双酶切与退火后的寡核苷酸... 目的构建表达DREAM基因的小分子干扰RNA(siRNA)重组腺相关病毒(rAAV)载体,并观察感染PC12细胞后DREAM蛋白表达受抑制情况。方法设计并合成shRNA对应的2条互补的寡核苷酸链,pDC316-EGFP-U6质粒经BamHⅠ和HindⅢ双酶切与退火后的寡核苷酸连接,构建pDC316-EGFP-DREAMshRNA-U6质粒,以其为模板设计引物并引入EcoRⅠ和SalⅠ位点,PCR产物酶切后与pSANV2.0连接构建重组质粒pSANV2.0-DREAMshRNA-EGFP,酶切测序证实后,重组腺相关病毒介导将pSANV2.0-DREAMshRNA-EGFP感染PC12细胞,Western blot检测DREAM蛋白表达水平。结果经PCR、酶切及测序证实,重组腺相关病毒载体质粒pSANV2.0-DREAMshRNA-EGFP构建成功,包装成病毒感染PC12细胞48 h后,Western blot结果显示与对照组细胞相比,DREAM蛋白表达水平显著下降(P<0.05)。结论成功构建和筛选出介导特异性shRNA-DREAM的重组腺相关病毒载体。 展开更多
关键词 dream基因 RNA干扰 重组腺相关病毒载体
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携带DREAM基因shRNA的慢病毒载体的构建及其对坐骨神经缩窄损伤大鼠的镇痛作用 被引量:5
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作者 王云姣 程智刚 +5 位作者 于鹏 李靖贻 白念岳 贺正华 杨胜辉 郭曲练 《中南大学学报(医学版)》 CAS CSCD 北大核心 2009年第8期723-730,共8页
目的:构建含下游调控元件的拮抗分子(downstream regulatory element antagonistic modulator,DREAM)基因有效短发夹RNA(shRNA)的慢病毒载体,利用RNA干扰技术抑制DREAM基因表达,开展对神经病理性疼痛基因治疗的实验研究。方法:将靶向大... 目的:构建含下游调控元件的拮抗分子(downstream regulatory element antagonistic modulator,DREAM)基因有效短发夹RNA(shRNA)的慢病毒载体,利用RNA干扰技术抑制DREAM基因表达,开展对神经病理性疼痛基因治疗的实验研究。方法:将靶向大鼠DREAM基因的shRNA重组到慢病毒穿梭质粒中,将重组质粒pKCSHR-puro/GFP-DREAM和慢病毒包装系统共转染293T细胞包装成慢病毒,收集病毒上清并进行载体病毒滴度的测定。将纯种健康清洁级成年雄性SD大鼠36只随机分为6组:正常对照组(N)、假手术组(S),根据干预手段的不同将大鼠坐骨神经缩窄损伤(chronic constriction injury of sci-atic nerve,CCI)模型组分为4组:CCI(C0)组,CCI疼痛模型建立后不给任何干预措施,生理盐水对照(C1)组,空白载体对照(C2)组,LV-shRNADREAM慢病毒载体治疗(C3)组。该3组大鼠于CCI后第7天分别于蛛网膜下腔注射生理盐水、空白载体、LV-shRNADREAM慢病毒载体。观察各组大鼠术后3,7,10,14,21 d的疼痛行为学的改变。实验结束取腰段脊髓荧光显微镜检测绿色荧光蛋白(GFP)的表达,Realtime-PCR测定DREAM基因的mRNA含量、Western印迹测定DREAM蛋白含量。结果:测序分析证实载体构建成功,成功进行慢病毒包装,得到病毒液滴度为1.0×108IFU/mL慢病毒载体。鞘内注射LV-shRNADREAM慢病毒载体后,CCI大鼠热痛阈和机械痛阈的异常明显得到改善(P<0.01),腰段脊髓神经细胞DREAM基因mRNA和DREAM蛋白的表达均显著降低(P<0.01)。结论:成功构建了携带大鼠DREAM基因有效shRNA的慢病毒载体,慢病毒携带的短发夹干扰RNA能干扰脊髓中DREAM的表达,为神经病理性疼痛的治疗提供了实验基础。 展开更多
关键词 RNA干扰 神经病理性疼痛 下游调控元件拮抗分子 慢病毒载体
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慢病毒介导的DREAM沉默治疗在坐骨神经慢性压迫模型大鼠疼痛中的作用及其对GALR1的表达调控 被引量:2
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作者 潘冰冰 程智刚 +5 位作者 孔高茵 王云姣 肖丹 丁惠娟 赵媛 郭曲练 《中国现代医学杂志》 CAS CSCD 北大核心 2013年第32期7-12,共6页
目的甘丙肽受体1(galanin receptor 1,GALR1)信号通路在痛觉调节中发挥重要作用,该研究利用坐骨神经慢性压迫(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,探索慢病毒介导的DREAM沉默治疗在CCI模型大鼠疼痛中的作... 目的甘丙肽受体1(galanin receptor 1,GALR1)信号通路在痛觉调节中发挥重要作用,该研究利用坐骨神经慢性压迫(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,探索慢病毒介导的DREAM沉默治疗在CCI模型大鼠疼痛中的作用及其对GALR1的表达调控。方法选择健康雄性SD大鼠24只,随机分为4组(n=6):即RNA干扰组、空白载体组、单纯CCI组和正常对照组。鞘内置管前后分别测定基础痛阈,RNA干扰组和空白载体组于CCI后经微导管给予pKCSHR-Puro/GFP-DREAM慢病毒和空白载体,并测定腰段脊髓内下游调控元件拮抗分子(downstream regulatory element antagonist,DREAM)和GALR1的蛋白表达。结果大鼠痛阈的变化:手术同侧热痛阈和机械痛阈测定结果表明,CCI处理后,RNA干扰组、空白载体组及单纯CCI组在各个时间点热痛阈和机械痛阈较正常对照组均显著降低(P<0.01);RNA干扰组鞘内注射后较注射前痛阈显著升高,治疗后相同时间点RNA干扰组较空白载体组及单纯CCI组痛阈亦显著升高。Western blotting结果表明,与其他实验组对比,RNA干扰组的DREAM蛋白表达水平显著下调,而GALR1蛋白表达水平较空白载体组、单纯CCI组亦有显著下调。结论 DREAM可以调控GALR1的表达,提示甘丙肽受体1信号通路参与DREAM基因调节大鼠神经病理性疼痛。 展开更多
关键词 甘丙肽受体1(GALR1) 下游调控元件拮抗分子(dream) 坐骨神经慢性压迫 RNA干扰 神经痛
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DREAM/Calsenilin/KChIP3:神经系统的多功能蛋白 被引量:3
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作者 张瑛 王韵 《生理科学进展》 CAS CSCD 北大核心 2005年第3期199-203,共5页
新近发现的转录因子DREAM(downstreamregulatoryelementantagonistmodulator)可结合到基因(包括PPD、Hrk、cfos等)的DRE(downstreamregulatoryelement)位点,抑制基因转录。它是第一个已知的可以直接与DNA结合发挥转录抑制作用的Ca2+结... 新近发现的转录因子DREAM(downstreamregulatoryelementantagonistmodulator)可结合到基因(包括PPD、Hrk、cfos等)的DRE(downstreamregulatoryelement)位点,抑制基因转录。它是第一个已知的可以直接与DNA结合发挥转录抑制作用的Ca2+结合蛋白,为Ca2+调节基因表达除蛋白激酶/磷酸酶这条主要通路外提供了另一条通路。由于DREAM与另两个研究小组发现的蛋白calsenilin和KChIP3实为同一种物质,所以DREAM具有PS(presenilin)作用蛋白、Kv4通道调节蛋白和转录因子的多重功能特性。本文将就DREAM的分布、功能及其调控、DREAM与疼痛的关系作简要综述。 展开更多
关键词 dream/Calsenilin/KChlP3 钙离子 基因表达调控 强啡肽 疼痛
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基于DREAM算法的双超模型参数不确定性研究 被引量:2
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作者 曹玉涛 郭向红 杨玫 《水电能源科学》 北大核心 2017年第9期13-16,共4页
鉴于半干旱半湿润地区的流域水文模拟及预报一直是水文模型领域研究的重点和难点问题,基于山西省张峰水库1988~2007年间18场洪水资料,前12场用于率定模型参数,后6场用于检验模型,采用DREAM方法分析其双超模型参数的不确定性,并在此基础... 鉴于半干旱半湿润地区的流域水文模拟及预报一直是水文模型领域研究的重点和难点问题,基于山西省张峰水库1988~2007年间18场洪水资料,前12场用于率定模型参数,后6场用于检验模型,采用DREAM方法分析其双超模型参数的不确定性,并在此基础上分析由参数不确定性导致的模型输出的不确定性。结果表明,10个参数的后验分布峰值不明显,说明参数的不确定性较大;4个参数的后验分布为具有明显峰值的不规则分布,7个参数的后验分布呈现偏态分布,其峰值也比较明显,说明该参数的不确定性较小。研究结果可为双超模型不确定性分析提供一定的理论基础。 展开更多
关键词 水文模型 dream算法 不确定性 双超模型 张峰水库
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