Meta-analysis of the curative efficacy of Duhuo Jisheng Decoction combined with western medicine on knee osteoarthritis

Objective:To evaluate the efficacy and safety of Duhuo Jisheng Decoction combined with western medicine in the treatment of knee osteoarthritis.Methods:The databases of CNKI,VIP,CBM,WANFANG DATA and PubMed were electronically to collect randomized controlled trials(RCTs)of the treatment of knee osteoarthritis with Duhuo Jisheng Decoction combined with western medicine.After literature was strictly screened and the study quality was evaluated according to relevant procedures,the Meta analysis was performed by RevMan5.3 software.Resluts:A total of 15 RCTs were included.The result of meta-analysis showed that:compared with the western medicine alone,Duhuo Jisheng Tang combined with western medicine could significantly improve the efficacy(RR=1.25,95%CI(1.18,1.32),p<0.00001),the posttreatment pain visual analogue scale scores(MD=-0.85,95%CI(-1.00,-0.71),p<0.00001)、the western ontario and mcmaster universities arthritis index scores(MD=-7.76,95%CI(-10.37,-5.15),p<0.00001)and matrix metalloproteinase3(MD=-11.72,95%CI(-17.39,-6.05),p<0.0001)were lower in treatment group compared to control group.There was no statistical difference in the incidence of adverse reactions between the 2groups.Conclusions:The current evidence shows that Duhuo Jisheng Decoction combined with western medicine can further improve the clinical efficacy for knee osteoarthritis.

Mechanism of Duhuo Jisheng Decoction based on bioinformatics and network pharmacology in treating osteoporosis of kidney Yin deficiency

Objective:To study the active ingredients,target and mechanism of Duhuo JiSheng Decoction for the treatment of osteoporosis by bioinformatics analysis and network pharmacology.Methods:The gene expression profile of GSE56116 was acquired through the NCBI GEO database,and the software R was used for differential analysis.The Chinese medicine system pharmacology technology platform(TCMSP)was used to retrieve the chemical components,target genes of Duhuo,Fangfeng,Xixin,and Sangjisheng.The differential genes acquired from GSE56116 were intersected and analyzed by GO enrichment analysis and KEGG pathway to study the mechanism of Duhuo JiSheng Decoction.Results:A total of 33 active ingredients and 9 differential genes of Duhuo JiSheng Decoction for the treatment of Shengyin deficiency osteoporosis were screened.GO function enrichment analysis focuses on the regulation of intracellular signal transduction,calcium-mediated signaling,and steroid hormone mediated signaling.KEGG analysis showed that it was mainly concentrated in signal pathways such as P53 signal pathway,TNF signal pathway,and IL-17 signal pathway.Conclusion:Through bioinformatics and network pharmacological analysis,Duhuo JiShenng Decoction may exert therapeutic effects by acting on AR,IGFBP3,and FOS target genes.