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On the Phenomenon of Information Silo in the E- government Construction in China
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作者 Li Jing 《学术界》 CSSCI 北大核心 2015年第1期312-316,共5页
In the past decade,the e-government affairs keep developing,which prom otes the reform of governmental affairs and the enhancement of administrative efficiency.However,the phenomenon of inform ation silo has bad influ... In the past decade,the e-government affairs keep developing,which prom otes the reform of governmental affairs and the enhancement of administrative efficiency.However,the phenomenon of inform ation silo has bad influence upon the governm ental information resources sharing and the efficiency of the e-government affairs,hindering inform ation connectivity,so that the government management cannot meet the need of society,nor can the e-governm ent develop in the long run.This paper attempts to analyze the embodiment and causes of the phenomenon of information silo,putting forward measures of solving the problem of inform ation silo. 展开更多
关键词 电子政务建设 信息孤岛 政府管理 中国 行政效率 资源共享 电子商务 信息化
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Reinforcing of Citizen’s Trust in E-Government: The Cameroon’s Case
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作者 Patrick Dany Bavoua Kenfack Check Njei 《Journal of Computer and Communications》 2024年第1期77-109,共33页
The embracing of ICTs and related technologies has enhanced different approaches for governments worldwide to deliver services to their citizens in a smart way. However, the usage of e-government services by common ci... The embracing of ICTs and related technologies has enhanced different approaches for governments worldwide to deliver services to their citizens in a smart way. However, the usage of e-government services by common citizens is recognized as one of the major setbacks of e-government development in both developed and developing countries. Moreover, government agencies in these countries are facing great challenges in keeping the citizens motivated enough to continue to use e-government services. This research aims to investigate the factors that influence citizens’ trust towards continue use of e-government services in Cameroon. The proposed research model consisted of three main constructs including technological, governmental, risk factors as well as six demographic characteristics (age, gender, educational level, income, internet experience and cultural perception). A five-point Likert scale questionnaire was designed to collect data physically and electronically, 352 valid questionnaires were retrieved. Simple and Multiple regression analysis methods were applied to build an adequate model based on the verification of hypotheses proposed. Based on results obtained, four demographic characteristics (age, education, occupation and income) have influence on citizens’ trust in e-government meanwhile gender and cultural affiliation have no influence. Furthermore, technological factors and governmental factors positively influence trust level in e-government, whereas risk factors have a negative influence on trust level. Deducing from the results, a list of recommendations is proposed to the government of Cameroon in order to reinforce citizens’ trust in e-government services. 展开更多
关键词 e-government Risk Factors Technological Factors governmental Factors TRUST Linear Regression
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Aspirations on the Bright Side of Humanity: It is Time to Translate Aspirations Into Actions With Establishing the Government of Earth 被引量:2
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作者 Nandor Ludvig 《Philosophy Study》 2024年第1期1-8,共8页
The thesis of this paper is that the digital noosphere of the World Wide Web and its users,the borderless move of virtually all resources of the planet,and the consequent globalization of most aspects of national poli... The thesis of this paper is that the digital noosphere of the World Wide Web and its users,the borderless move of virtually all resources of the planet,and the consequent globalization of most aspects of national politics,economy,and culture,along with the increasing difficulty of controlling the related high-tech military conflicts,human-made environmental crises,and the abnormal migrations of abused people now make it inevitable to leave behind the competitive international chaos rooted in humankind’s animal past and turn to the cooperative social spirit recommended by Aristotle,Lao Tzu,Jesus,Dante,H.G.Wells,Martin Luther King Jr.,John Lennon,Nicholas Hagger,and others to finally translate their aspirations into actions via establishing the Government of Earth for the decent and free.The paper presents a five-step,15-year,~$25B plan to start this process.That this plan has nothing to do with the Godless work of secret societies,the authoritarian urges of renewed fascism or the political absurdities of Marxism-Leninism it is relayed by the envisioned Intercontinental Education System of the outlined Government of Earth to teach every child on the planet-via a new community of digitally linked extraordinary teachers-for the beauty of lifelong learning,tolerance,duty,honor,free cooperation,and artful communication for divine missions. 展开更多
关键词 humankind Soul of Multiverse cosmological neuroscience government of Earth
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Inter-agency government information sharing under data-driven blockchain framework
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作者 XIAO Jiong-en HONG Ming DING Li-ping 《控制理论与应用》 EI CAS CSCD 北大核心 2024年第8期1369-1376,共8页
The inter-agency government information sharing(IAGIS)plays an important role in improving service and efficiency of government agencies.Currently,there is still no effective and secure way for data-driven IAGIS to fu... The inter-agency government information sharing(IAGIS)plays an important role in improving service and efficiency of government agencies.Currently,there is still no effective and secure way for data-driven IAGIS to fulfill dynamic demands of information sharing between government agencies.Motivated by blockchain and data mining,a data-driven framework is proposed for IAGIS in this paper.Firstly,the blockchain is used as the core to design the whole framework for monitoring and preventing leakage and abuse of government information,in order to guarantee information security.Secondly,a four-layer architecture is designed for implementing the proposed framework.Thirdly,the classical data mining algorithms PageRank and Apriori are applied to dynamically design smart contracts for information sharing,for the purposed of flexibly adjusting the information sharing strategies according to the practical demands of government agencies for public management and public service.Finally,a case study is presented to illustrate the operation of the proposed framework. 展开更多
关键词 government data processing blockchain PAGERANK APRIORI
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Influence mechanism of government subsidy on the green transformation of coal company in China
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作者 Xiaolei Li Changsong Wu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第7期1033-1040,共8页
The optimization of government subsidies to enhance the efficiency of coal companies’green transformation constitutes a critical component in the pursuit of global sustainability.We investigate the influence mechanis... The optimization of government subsidies to enhance the efficiency of coal companies’green transformation constitutes a critical component in the pursuit of global sustainability.We investigate the influence mechanism of government subsidies on the green transformation using data from the listed coal companies in China from 2007 to 2022.According to our findings and hypothesis testing,previous government subsidies did not have a significant direct impact on coal companies’green transformation.Nevertheless,government subsidies can help coal companies transition to greener practices by promoting innovative green initiatives.Furthermore,we confirmed an indirect route:that government subsidies enable the adoption of low-carbon initiatives,which in turn could facilitate the transition of coal companies towards green practices.In addition,we discovered that the coal company’s digitization will improve this indirect route.Thus,we propose increasing the effectiveness of government subsidies in facilitating coal companies’transition to green practices by focusing on technological advancements and enhancing company digitalization. 展开更多
关键词 government subsidy Green transformation Coal company DIGITALIZATION INNOVATION
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Constructing a Triangular Linkage Model of Government Audit,Social Audit,and Internal Audit to Promote High-Quality Development of Audit Services
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作者 TANG Pengzhan 《Journal of Modern Accounting and Auditing》 2024年第3期89-98,共10页
It is a complex and important topic to study the linkage mechanism of government audit,social audit,and internal audit in the context of China’s high-quality economic development.The implementation of measures,such a... It is a complex and important topic to study the linkage mechanism of government audit,social audit,and internal audit in the context of China’s high-quality economic development.The implementation of measures,such as establishing a sound and perfect organizational safeguard mechanism,strengthening project collaborative audit mechanism,enhancing the mechanism for utilizing audit results,and establishing an audit and rectification joint mechanism can promote the efficient operation of the audit supervision system and the high-quality development of audit services. 展开更多
关键词 government audit social audit internal audit triangular linkage model high-quality development
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The Impact of Transformational Leadership on Commitment to Change Among Jordanian Government Accountant Toward IPSAS Implementation
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作者 Mohammad Abu Zraiq Rosnia Masruki 《Journal of Modern Accounting and Auditing》 2024年第3期73-88,共16页
IPSAS was accepted by Jordan’s government in accordance with worldwide trends.The Jordanian Ministry of Finance launched cash-basis IPSAS in 2015 and planned to implement accrual accounting by January 1,2021.However,... IPSAS was accepted by Jordan’s government in accordance with worldwide trends.The Jordanian Ministry of Finance launched cash-basis IPSAS in 2015 and planned to implement accrual accounting by January 1,2021.However,the commitment to change remains uneven,hindering the full shift.Moving from old accounting processes to new ones is tough.Even then,it hasn’t been implemented,creating a gap due to the difficulty in committing to new accounting standards throughout implementation due to obstacles.Thus,knowing government accountants’issues is essential to applying IPSAS in government accounting.This study examines how transformational leadership affects government accountants’commitment to IPSAS adoption in Jordan’s public sector.This study used a quantitative approach to survey Jordanian Ministry of Finance accountants.The 384-person study had a 78%response rate.Additionally,PLS-SEM was used to confirm variable relationships.Transformational leadership positively predicted IPSAS implementation,according to the study. 展开更多
关键词 IPSAS commitment to change transformational leadership government accountant Ministry of Finance Jordan
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Exploration of the Impact Mechanism of Government Credibility Based on Variable Screening Method
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作者 Jiajun Wu Yuxiang Ma +2 位作者 Helin Zou Chun Zhang Ran Yan 《Journal of Data Analysis and Information Processing》 2024年第3期479-494,共16页
Government credibility is an important asset of contemporary national governance, an important criterion for evaluating government legitimacy, and a key factor in measuring the effectiveness of government governance. ... Government credibility is an important asset of contemporary national governance, an important criterion for evaluating government legitimacy, and a key factor in measuring the effectiveness of government governance. In recent years, researchers’ research on government credibility has mostly focused on exploring theories and mechanisms, with little empirical research on this topic. This article intends to apply variable selection models in the field of social statistics to the issue of government credibility, in order to achieve empirical research on government credibility and explore its core influencing factors from a statistical perspective. Specifically, this article intends to use four regression-analysis-based methods and three random-forest-based methods to study the influencing factors of government credibility in various provinces in China, and compare the performance of these seven variable selection methods in different dimensions. The research results show that there are certain differences in simplicity, accuracy, and variable importance ranking among different variable selection methods, which present different importance in the study of government credibility issues. This study provides a methodological reference for variable selection models in the field of social science research, and also offers a multidimensional comparative perspective for analyzing the influencing factors of government credibility. 展开更多
关键词 government Credibility Variable Selection Models Social Statistics Regression Based Approach Method Based on Random Forest
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Research on the Relationship Between Average Cigarette Price per Box and Government Procurement in City A Based on a Regression Model
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作者 Yao Nie Hongbo Wan Mingming Mao 《Proceedings of Business and Economic Studies》 2024年第5期68-72,共5页
This study aims to analyze and predict the relationship between the average price per box in the cigarette market of City A and government procurement,providing a scientific basis and support for decision-making.By re... This study aims to analyze and predict the relationship between the average price per box in the cigarette market of City A and government procurement,providing a scientific basis and support for decision-making.By reviewing relevant theories and literature,qualitative prediction methods,regression prediction models,and other related theories were explored.Through the analysis of annual cigarette sales data and government procurement data in City A,a comprehensive understanding of the development of the tobacco industry and the economic trends of tobacco companies in the county was obtained.By predicting and analyzing the average price per box of cigarette sales across different years,corresponding prediction results were derived and compared with actual sales data.The prediction results indicate that the correlation coefficient between the average price per box of cigarette sales and government procurement is 0.982,implying that government procurement accounts for 96.4%of the changes in the average price per box of cigarettes.These findings offer an in-depth exploration of the relationship between the average price per box of cigarettes in City A and government procurement,providing a scientific foundation for corporate decision-making and market operations. 展开更多
关键词 Cigarette marketing Regression model Predictive model government purchasing
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Analysis of Influencing Factors of Work Motivation of Government Employees Based on MaxDiff Model:Evidence from Jiangmen City
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作者 Weijie Chen 《Proceedings of Business and Economic Studies》 2024年第4期226-230,共5页
The purpose of this paper is to use the MaxDiff model to explore the factors influencing the work motivation of staff in certain government agencies in Jiangmen City,and to conduct relevant empirical analyses.The stud... The purpose of this paper is to use the MaxDiff model to explore the factors influencing the work motivation of staff in certain government agencies in Jiangmen City,and to conduct relevant empirical analyses.The study aims to propose strategies for improving work motivation among these staff members.The results indicate that the most significant factors affecting the work motivation of staff in Jiangmen government agencies are salary and remuneration,additional welfare benefits,and the power to make decisions about their own work.In contrast,factors such as a sense of belonging to the work unit,recognition of their work,and job security are less important.Therefore,it is recommended that government agencies in Jiangmen City increase the salaries and benefits of their staff and provide them with more decision-making power.This approach will stimulate their desire to work,enhance their motivation,improve the efficiency of government agencies,and elevate the level of public service.Ultimately,these measures will support the construction of a harmonious,stable,and orderly society. 展开更多
关键词 government agency workers Job motivation MaxDiff model
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Research on the Relationship between the School-Running Characteristics of Private Colleges and Universities and the Development Objectives of the Government
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作者 Lishuxian Zhao Xiaoxia Wu Zhuolin Zhu 《Journal of Contemporary Educational Research》 2024年第9期296-302,共7页
The development objectives of the Chinese government have a guiding effect on the school-running characteristics of private colleges and universities.This paper explores the relationship between the characteristics of... The development objectives of the Chinese government have a guiding effect on the school-running characteristics of private colleges and universities.This paper explores the relationship between the characteristics of private colleges and universities and the development objectives of the provincial government,using Jiangsu Province as a case study.All the schools have the consciousness of fully considering the development objectives of Jiangsu Province when formulating the school-running characteristics.Their characteristics all contain some training methods,training courses,or institutional construction aiming at the development objectives.Most universities can support the government’s objectives on employment and innovation.Only a third of the schools provide government support for science research.Private colleges and universities should make full use of the free and flexible advantages of private education,combine them with the continuous changes of policies and markets,and create clear,scientific,and dynamic school-running characteristics,in order to achieve long-term development. 展开更多
关键词 Development objectives of the government School-running characteristics Chinese Private colleges and universities
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Effective Utilization of Government-Provided CTI by Small Businesses within the Defense Industrial Base
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作者 Josiah Dykstra Lawrence A. Gordon +2 位作者 Martin P. Loeb Benjamin Wall Lei Zhou 《Journal of Information Security》 2024年第2期196-217,共22页
There are two broad objectives of the research reported in this paper. First, we assess whether government-provided cyber threat intelligence (CTI) is helpful in preventing, or responding to, cyber-attacks among small... There are two broad objectives of the research reported in this paper. First, we assess whether government-provided cyber threat intelligence (CTI) is helpful in preventing, or responding to, cyber-attacks among small businesses within the U.S. Defense Industrial Base (DIB). Second, we identify ways of improving the effectiveness of government-provided CTI to small businesses within the DIB. Based on a questionnaire-based survey, our findings suggest that government-provided CTI helps businesses within the DIB in preventing, or responding to, cyber-attacks providing a firm is familiar with the CTI. Unfortunately, a large percentage of small firms are not familiar with the government-provided CTI feeds and consequently are not utilizing the CTI. This latter situation is largely due to financial constraints confronting small businesses that prevent firms from having the wherewithal necessary to effectively utilize the government-provided CTI. However, we found a significant positive association between a firm’s familiarity with the government-provided CTI and whether a firm is being periodically reviewed by the Defense Counterintelligence and Security Agency (DCSA) or is compliant with the Cybersecurity Maturity Model Certification (CMMC) program. The findings from our study also show that the participating firms believe that external cyber threats are more likely to be the cause of a future cybersecurity breach than internal cybersecurity threats. Finally, our study found that the portion of the IT budget that small businesses within the DIB spend on cybersecurity-related activities is dependent on the perception that a firm would be the target of an external cyber-attack. 展开更多
关键词 government-Provided CTI Small Businesses Defense Industrial Base
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A Role-Based PMI Security Model for E-Government 被引量:2
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作者 WULi-jun SUKai-le YANGZhi-hua 《Wuhan University Journal of Natural Sciences》 CAS 2005年第1期329-332,共4页
We introduce the general AC( atlribure certificate), the role specificationAC and the rolt assignment AC We discuss the rolt-based PMI(Privilege Management Infrastructure)architecture. The role-based PMT(Public-Kty In... We introduce the general AC( atlribure certificate), the role specificationAC and the rolt assignment AC We discuss the rolt-based PMI(Privilege Management Infrastructure)architecture. The role-based PMT(Public-Kty In-frastructure) secure model forE-govcrnment isresearehed by combining the role-bastd PMI with PKI architeclure (Public Key Infrastructure). Themodel has advantages of flexibility, convenience, less storage space and less network consumptionetc. We are going to ust iht secure modelin the E-govern-ment system. 展开更多
关键词 e-govemment public key certificate role-specification AC role-assignmentAC ihe security model for e- government
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组织中MMP-9、E-cadherin与子宫内膜样癌伴MELF浸润方式及肿瘤侵袭程度的关系
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作者 李坤 王静 +2 位作者 金春亭 白睿 刘晓娟 《河北医科大学学报》 CAS 2024年第8期980-986,共7页
目的分析子宫内膜样癌组织中基质金属蛋白酶(matrix metalloproteinase,MMP)-9、E-钙黏蛋白(E-cadherin)与微囊性、伸长及碎片状(microcystic,elongation,and fragmentation,MELF)浸润方式及肿瘤侵袭程度之间的关系,探讨能够识别子宫内... 目的分析子宫内膜样癌组织中基质金属蛋白酶(matrix metalloproteinase,MMP)-9、E-钙黏蛋白(E-cadherin)与微囊性、伸长及碎片状(microcystic,elongation,and fragmentation,MELF)浸润方式及肿瘤侵袭程度之间的关系,探讨能够识别子宫内膜样癌伴MELF浸润的指标,抑制肿瘤侵袭能力。方法回顾性分析在河北北方学院附属第一医院接受手术治疗的112例子宫内膜样癌患者临床资料,全部患者均顺利完成改良根治性子宫切除术,并获得肿瘤标准,根据病理检查结果对112例患者进行分组,分为MELF组和N-MELF组。统计112例患者一般临床资料,血液检查项目包括肿瘤标志物,免疫组织化学检查项目包括组织中MMP-9、E-cadherin,根据检查结果,构建Logistic回归模型,分析组织中MMP-9、E-cadherin及相关指标与子宫内膜样癌MELF浸润方式及肿瘤侵袭程度的关系。结果研究中112例子宫内膜样癌患者,评估为MELF浸润患者21例(18.75%);MELF浸润患者病理等级低级别,国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)ⅣA期,淋巴脉管间隙浸润(lymphatic vascular space,LVSI)、组织中MMP-9阳性患者占比多于N-MELF浸润患者,E-cadherin阳性患者占比少于N-MELF浸润患者,人附睾蛋白(humanepididymisprotein 4,HE4),甲壳质酶蛋白(human cartilageglycopmtein,YKL-40)水平高于N-MELF患者,差异有统计学意义(P<0.05)。多元Logistic回归分析提示,组织中E-cadherin表达率低、MMP-9表达率高,MELF浸润与子宫内膜样癌FIGO分期进展有关(P<0.05)。结论子宫内膜样癌发生MELF浸润与组织中MMP-9、E-cadherin有关,MMP-9表达率高和E-cadherin表达率低能够通过导致MELF浸润发生,从而增强肿瘤侵袭能力。 展开更多
关键词 子宫内膜样癌 基质金属蛋白酶-9 e-钙黏蛋白
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血清ESM-1、E-cad对ACI-LAA溶栓后HT的预测价值
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作者 茹姗 邹婷 +2 位作者 杜磊 韩登峰 马建华 《脑与神经疾病杂志》 CAS 2024年第2期96-99,共4页
目的 探讨血清内皮细胞特异性分子-1 (ESM-1)、E-钙黏连蛋白(E-cad)对大动脉粥样硬化型脑梗死(ACI-LAA)溶栓后出血转化(HT)的预测价值。方法 选取2020年1月至2022年12月新疆医科大学第一附属医院收治的110例ACI-LAA患者,根据是否发生HT... 目的 探讨血清内皮细胞特异性分子-1 (ESM-1)、E-钙黏连蛋白(E-cad)对大动脉粥样硬化型脑梗死(ACI-LAA)溶栓后出血转化(HT)的预测价值。方法 选取2020年1月至2022年12月新疆医科大学第一附属医院收治的110例ACI-LAA患者,根据是否发生HT分为HT组和non-HT组。对比两组基础资料及血清ESM-1、E-cad水平。采用ROC曲线分析ESM-1、E-cad预测ACI-LAA患者发生HT的价值。结果 HT组患者NIHSS评分高于non-HT组,梗死面积大于non-HT组(P <0.05),HT组患者血清ESM-1、E-cad水平也高于non-HT组(P<0.05)。Logistic回归分析显示,梗死面积大、NIHSS评分高、血清ESM-1和E-cad水平升高是ACI-LAA患者发生HT的危险因素(P<0.05)。ESM-1联合E-cad预测ACI-LAA患者发生HT的曲线下面积为0.859,预测灵敏度为84.6%,特异度为72.6%。结论 血清ESM-1和E-cad水平与ACI-LAA患者HT密切相关,可作为早期预测发生HT的参考指标。 展开更多
关键词 动脉粥样硬化 脑梗死 出血转化 内皮细胞特异性分子-1 e-钙黏连蛋白
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MMP25和E-cadherin在类风湿关节炎中的诊断价值
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作者 方全钢 刘海芸 +1 位作者 万静 张水生 《江西医药》 CAS 2024年第2期143-147,154,共6页
目的探讨基质金属蛋白酶25(Matrix metalloproteinase 25,MMP25)和E-钙黏蛋白(E-cadherin)对类风湿关节炎的诊断意义。方法选取2020年1月至2023年10月我院67例类风湿关节炎患者为实验组,同期50例健康体检人群为对照组;采用酶联免疫吸附... 目的探讨基质金属蛋白酶25(Matrix metalloproteinase 25,MMP25)和E-钙黏蛋白(E-cadherin)对类风湿关节炎的诊断意义。方法选取2020年1月至2023年10月我院67例类风湿关节炎患者为实验组,同期50例健康体检人群为对照组;采用酶联免疫吸附试验(ELISA)检测二组血清中MMP25和E-cadherin蛋白水平。收集实验组患者ESR、CRP、CCP、RF、DAS28-ESR、DAS28-CRP指标,以及是否为初诊、是否合并并发症(贫血、间质性肺炎等)、病程、临床活动度等级,并与MMP25和E-cadherin水平进行相关性分析。结果实验组血清MMP25和E-cadherin水平均高于对照组,差异均有统计学意义(P<0.001)。与ESR、CRP、CCP和RF等指标进行散点图和简单线性回归性分析,结果显示:不同临床活动度患者表达MMP25水平差异有统计学意义(P<0.001),MMP25与病程(β=0.479,P<0.001),MMP25与CRP(β=0.271,P<0.05),MMP25与DAS28-ESR(β=0.660,P<0.001)和MMP25与DAS28-CRP(β=0.624,P<0.001)均有相关性。不同临床活动度患者表达E-cadherin水平差异有统计学意义(P<0.001),E-cadherin与ESR(β=0.425,P<0.001),E-cadherin与CRP(β=0.328,P<0.05),E-cadherin与DAS28-ESR(β=0.746,P<0.001),E-cadherin与DAS28-CRP(β=0.671,P<0.001)均有相关性。多重线性回归分析结果显示,MMP25表达水平与临床活动度正相关(β=1.032,P<0.001),E-cadherin表达水平与临床活动度正相关(β=0.407,P<0.05)、与病程长短正相关(β=0.497,P<0.001)。结论MMP25和E-cadherin表达水平可以作为类风湿关节炎评估临床活动度的标志物,此外,E-cadherin表达水平也可以衡量患者病程长短。 展开更多
关键词 基质金属蛋白酶25 e-钙黏蛋白 类风湿关节炎 诊断价值
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miR-200c/ZEB1/E-cadherin轴在胆管癌转移侵袭中的作用及对胆管癌预后评估的价值
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作者 潘超 许蔚 +2 位作者 刘子君 陶平 杨士勇 《现代肿瘤医学》 CAS 2024年第7期1287-1294,共8页
目的:探讨微小RNA-200c(miR-200c)/锌指转录因子(ZEB1)/E-钙黏蛋白(E-cadherin)在胆管癌(HCCA)转移、侵袭中的作用及对HCCA预后评估的临床价值。方法:选取2020年08月至2022年12月间我院收治的胆管癌患者36例为研究对象,同时选取胆管良... 目的:探讨微小RNA-200c(miR-200c)/锌指转录因子(ZEB1)/E-钙黏蛋白(E-cadherin)在胆管癌(HCCA)转移、侵袭中的作用及对HCCA预后评估的临床价值。方法:选取2020年08月至2022年12月间我院收治的胆管癌患者36例为研究对象,同时选取胆管良性疾病患者(胆总管结石或胆管良性狭窄)20例作为对照。采用原位杂交、实时荧光定量PCR检测miR-200c在HCCA患者组织及血清中的表达;生存曲线分析miR-200c表达与总生存期的相关性;Transwell实验检测细胞的迁移及侵袭能力;免疫印迹检测ZEB1及E-cadherin蛋白的表达。体内研究构建裸鼠成瘤实验,采用免疫组化检测ZEB1及E-cadherin蛋白的表达;Tunel技术检测组织中的凋亡情况。受试者工作特征(ROC)曲线评估生物标志物对胆管癌预后的诊断效能。结果:miR-200c低表达于HCCA组织及血清中;生存曲线分析显示,miR-200c表达与患者的总生存期呈正相关性,TNM分期与患者的总生存期呈负相关性;miR-200c mimics抑制了胆管癌细胞的迁移和侵袭能力,并降低ZEB1表达而诱导E-cadherin表达;转染ZEB1逆转miR-200c的作用效应,促进胆管癌细胞的迁移和侵袭特性,抑制胆管癌细胞的凋亡。CA199、CEA和miR-200c联合检测诊断胆管癌预后的ROC曲线下面积为0.892,灵敏度0.8611,特异度0.8000。结论:miR-200c/ZEB1/Ecadherin轴参与胆管癌的转移侵袭;CA199、CEA和miR-200c联合检测对胆管癌预后的诊断效能良好,对临床胆管癌的治疗策略具有重要指导价值。 展开更多
关键词 胆管癌 微小RNA-200c(miR-200c) 锌指转录因子(ZEB1) e-钙黏蛋白(e-cadherin) 迁移 侵袭
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中药砒霜上调E-钙黏蛋白抑制宫颈癌Hela细胞生长、侵袭、迁移机制研究
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作者 李思瑾 李海盈 +3 位作者 何泽阳 李安 张亚文 李道成 《新中医》 CAS 2024年第14期145-152,共8页
目的:研究中药砒霜的主要成分三氧化二砷(As_(2)O_(3))抑制宫颈癌Hela细胞生长、侵袭、迁移的机制,观察As_(2)O_(3)对宫颈癌Hela细胞组蛋白脱乙酰基酶1(HDAC1)、E-钙黏蛋白表达的影响,以及联合HDAC1抑制剂伏立诺他(SAHA)的协同作用。方... 目的:研究中药砒霜的主要成分三氧化二砷(As_(2)O_(3))抑制宫颈癌Hela细胞生长、侵袭、迁移的机制,观察As_(2)O_(3)对宫颈癌Hela细胞组蛋白脱乙酰基酶1(HDAC1)、E-钙黏蛋白表达的影响,以及联合HDAC1抑制剂伏立诺他(SAHA)的协同作用。方法:以宫颈癌Hela细胞作为载体,随机分为空白对照组、As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组4组,采用CCK-8法测定不同药物浓度对Hela细胞活性的抑制情况,并筛选后续实验药物浓度;采用Transwell法、划痕法检测各组药物对Hela细胞侵袭、迁移能力的影响;采用实时荧光定量聚合酶链式反应及蛋白免疫印迹法检测HDAC1和E-钙黏蛋白的mRNA表达情况与蛋白表达情况。结果:随着各组药物浓度增加,Hela细胞的活性逐渐降低。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组的Hela细胞活性均低于空白对照组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组穿越Transwell小室膜的细胞数量均少于空白对照组(P<0.05);As_(2)O_(3)+SAHA组的细胞数量少于As_(2)O_(3)组、SAHA组(P<0.05);As_(2)O_(3)组的细胞数量少于SAHA组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组的Hela细胞迁移百分比均低于空白对照组(P<0.05);As_(2)O_(3)+SAHA组的Hela细胞迁移百分比低于As_(2)O_(3)组、SAHA组(P<0.05);As_(2)O_(3)组的Hela细胞迁移百分比低于SAHA组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组的HDAC1 mRNA表达水平均低于空白对照组(P<0.05);As_(2)O_(3)组的HDAC1 mRNA表达水平高于SAHA组(P<0.05);As_(2)O_(3)+SAHA组的HDAC1 mRNA表达水平低于As_(2)O_(3)组、SAHA组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组的E-钙黏蛋白mRNA表达水平均高于空白对照组(P<0.05);As_(2)O_(3)+SAHA组的E-钙黏蛋白mRNA表达水平高于As_(2)O_(3)组、SAHA组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组的HDAC1蛋白表达水平均低于空白对照组(P<0.05);As_(2)O_(3)+SAHA组的HDAC1蛋白表达水平低于As_(2)O_(3)组、SAHA组(P<0.05)。As_(2)O_(3)组、SAHA组、As_(2)O_(3)+SAHA组E-钙黏蛋白的蛋白表达水平均高于空白对照组(P<0.05);As_(2)O_(3)+SAHA组E-钙黏蛋白的蛋白表达水平高于As_(2)O_(3)组、SAHA组(P<0.05)。结论:As_(2)O_(3)抑制Hela细胞的最佳浓度为8μmol/L,联合SAHA时,最佳抑制浓度为4μmol/L。As_(2)O_(3)可能通过抑制HDAC1的表达,同时促进升高E-钙黏蛋白的表达,与SAHA发挥协同作用抑制Hela细胞生长、侵袭、迁移。 展开更多
关键词 宫颈癌 HELA细胞 砒霜 三氧化二砷 伏立诺他 组蛋白脱乙酰基酶1 e-钙黏蛋白
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血清E-cadherin、VEGF、CYFRA21-1水平在甲状腺癌患者手术前后的变化及其对术后复发转移的预测价值
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作者 孙晓光 李彬 洪帅 《实用临床医学(江西)》 CAS 2024年第1期34-37,49,共5页
目的探讨血清E-钙粘连蛋白(E-cadherin)、血管内皮生长因子(VEGF)、细胞角蛋白19片段抗原21-1(CYFRA21-1)水平在甲状腺癌患者手术前后的变化及其对术后复发转移的预测价值。方法选取118例行甲状腺癌根治术的分化型甲状腺癌(DTC)患者为... 目的探讨血清E-钙粘连蛋白(E-cadherin)、血管内皮生长因子(VEGF)、细胞角蛋白19片段抗原21-1(CYFRA21-1)水平在甲状腺癌患者手术前后的变化及其对术后复发转移的预测价值。方法选取118例行甲状腺癌根治术的分化型甲状腺癌(DTC)患者为研究组,118例健康体检者为对照组;根据术后6个月复发转移情况,将DTC患者分为复发转移组33例与未复发转移组85例。比较对照组与研究组(术前)、研究组手术前后、复发转移组与未复发转移组血清E-cadherin、VEGF、CYFRA21-1水平,采用ROC曲线分析上述各指标对术后复发转移的预测价值。结果研究组血清E-cadherin水平显著低于对照组,血清VEGF、CYFRA21-1水平显著高于对照组(P<0.05);研究组术后2周血清E-cadherin水平显著高于术前,血清VEGF、CYFRA21-1水平显著低于术前(P<0.05);未复发转移组术后2周血清E-cadherin水平显著高于术后复发转移组,血清VEGF、CYFRA21-1水平显著低于术后复发转移组(P<0.05)。术后2周上述各指标联合检测预测DTC患者术后复发转移的AUC为0.862,敏感度为90.91%,特异度为75.29%。结论DTC患者术后血清中E-cadherin表达上调,VEGF、CYFRA21-1表达下调,各指标联合检测对复发转移具有较高的预测效能,可作为临床评估DTC患者术后复发转移情况的辅助指标。 展开更多
关键词 e-钙粘连蛋白 血管内皮生长因子 细胞角蛋白19片段抗原21-1 甲状腺癌 复发转移
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血清人软骨糖蛋白-39、可溶性E-选择素与儿童湿疹及严重程度的相关性
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作者 张辉 杜凡 李燕妮 《安徽医药》 CAS 2024年第11期2197-2201,共5页
目的探究血清人软骨糖蛋白-39(YKL-40)、可溶性E-选择素(E-selectin)与儿童湿疹的相关性及与湿疹严重程度的关系。方法选取2019年3月至2020年3月延安大学附属医院收治的湿疹病儿120例为观察组,根据湿疹面积及严重程度指数(EASI)评分将... 目的探究血清人软骨糖蛋白-39(YKL-40)、可溶性E-选择素(E-selectin)与儿童湿疹的相关性及与湿疹严重程度的关系。方法选取2019年3月至2020年3月延安大学附属医院收治的湿疹病儿120例为观察组,根据湿疹面积及严重程度指数(EASI)评分将病儿分为轻度组(42例)、中度组(40例)和重度组(38例)。选取同期进行体检者50例作为对照组。检测血清YKL-40、E-selectin水平;比较临床资料;采用Pearson法分析观察组YKL-40、E-selectin水平的相关性;Spearman法分析血清YKL-40、E-selectin水平与病情严重程度的相关性;logistic分析发生重度湿疹的影响因素。结果观察组年龄和性别与对照组比较均差异无统计学意义(P>0.05),而观察组病儿在过敏性鼻炎[18.33%(32/120)]、过敏史[65.00%(78/120)]、糖尿病家族史[57.50%(69/120)]、YKL-40[(38.05±4.80)g/L]和E-selectin水平[(64.12±6.83)μg/L]上与对照组[10.00%(6/50)、28.00%(14/50)、38.00%(19/50)、(24.69±3.44)g/L、(56.38±6.18)μg/L]差异有统计学意义(t/χ^(2)=4.37、19.46、5.38、17.85、6.92,P<0.05);轻度组、中度组和重度组的YKL-40[(30.95±3.51)g/L、(38.28±4.69)g/L、(45.66±6.34)g/L]和E-selectin水平[(58.82±6.05)μg/L、(63.96±6.85)μg/L、(70.15±7.68)μg/L]均依次显著升高,差异有统计学意义(F=88.67、26.71,P<0.05);观察组血清YKL-40、E-selectin呈正相关(r=0.68,P=0.001);血清YKL-40、E-selectin与病情严重程度呈正相关(r=0.68、0.58,均P<0.001);过敏史、糖尿病家族史、YKL-40和E-selectin为患湿疹严重程度的独立危险因素(P<0.05)。结论血清YKL-40和E-selectin水平升高,湿疹病儿病情可能会加重,二者均为湿疹严重程度的独立危险因素。 展开更多
关键词 湿疹 人软骨蛋白-39 可溶性e-选择素 病情严重程度
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