Elaidic acid(EA)stimulation can lead to endoplasmic reticulum stress(ERS),accompanied by a large release of Ca^(2+),and ultimately the activation of NLRP3 inflammasome in Kupffer cells(KCs).Mitochondrial instability o...Elaidic acid(EA)stimulation can lead to endoplasmic reticulum stress(ERS),accompanied by a large release of Ca^(2+),and ultimately the activation of NLRP3 inflammasome in Kupffer cells(KCs).Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome,and sustained Ca^(2+)transfer can result in mitochondrial dysfunction.We focused on KCs to explore the damage to mitochondria by EA.After EA stimulation,cells produced an oxidative stress(OS)response with a significant increase in ROS release.Immunoprecipitation experiments and the addition of inhibitors revealed that the increase in the level of intracellular Ca^(2+)led to Ca^(2+)accumulation in the mitochondrial matrix via mitochondria-associated membranes(MAMs).This was accompanied by a significant release of m ROS,loss of MMP and ATP,and a significant increase in mitochondrial permeability transition pore opening,ultimately leading to mitochondrial instability.These findings confirmed the mechanism that EA induced mitochondrial Ca^(2+)imbalance in KCs via MAM,ultimately leading to mitochondrial dysfunction.Meanwhile,EA induced OS and the decrease of MMP and ATP in rat liver,and significant lesions were found in liver mitochondria.Swelling of the inner mitochondrial cristae and mitochondrial vacuolization occurred,with a marked increase in lipid droplets.展开更多
Effective English reading requires readers not only possess good English grammar, flexible reading skills and plentiful vocabulary but also master rich cultural background knowledge.However, for the adult students edu...Effective English reading requires readers not only possess good English grammar, flexible reading skills and plentiful vocabulary but also master rich cultural background knowledge.However, for the adult students educated in the Continuing Education College, it is rather obvious that they have troubles learning English The author of this thesis pro-posed a new idea of English reading teaching-teaching with allusions and their stories in English reading class.The author tries to apply the relationship between schema theory and reading in the process of teaching adult students so that they can get a deeper understanding of English allusions and their stories.The author of this thesis did an empirical research and made a quantitative analysis for pretest and posttest.The result of this research indicates that to improve English reading proficiency, either teachers or students should attach equal importance both to language structures and to cultural background knowledge.展开更多
基金supported by fund from the National Natural Science Foundation of China(32172322)。
文摘Elaidic acid(EA)stimulation can lead to endoplasmic reticulum stress(ERS),accompanied by a large release of Ca^(2+),and ultimately the activation of NLRP3 inflammasome in Kupffer cells(KCs).Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome,and sustained Ca^(2+)transfer can result in mitochondrial dysfunction.We focused on KCs to explore the damage to mitochondria by EA.After EA stimulation,cells produced an oxidative stress(OS)response with a significant increase in ROS release.Immunoprecipitation experiments and the addition of inhibitors revealed that the increase in the level of intracellular Ca^(2+)led to Ca^(2+)accumulation in the mitochondrial matrix via mitochondria-associated membranes(MAMs).This was accompanied by a significant release of m ROS,loss of MMP and ATP,and a significant increase in mitochondrial permeability transition pore opening,ultimately leading to mitochondrial instability.These findings confirmed the mechanism that EA induced mitochondrial Ca^(2+)imbalance in KCs via MAM,ultimately leading to mitochondrial dysfunction.Meanwhile,EA induced OS and the decrease of MMP and ATP in rat liver,and significant lesions were found in liver mitochondria.Swelling of the inner mitochondrial cristae and mitochondrial vacuolization occurred,with a marked increase in lipid droplets.
文摘Effective English reading requires readers not only possess good English grammar, flexible reading skills and plentiful vocabulary but also master rich cultural background knowledge.However, for the adult students educated in the Continuing Education College, it is rather obvious that they have troubles learning English The author of this thesis pro-posed a new idea of English reading teaching-teaching with allusions and their stories in English reading class.The author tries to apply the relationship between schema theory and reading in the process of teaching adult students so that they can get a deeper understanding of English allusions and their stories.The author of this thesis did an empirical research and made a quantitative analysis for pretest and posttest.The result of this research indicates that to improve English reading proficiency, either teachers or students should attach equal importance both to language structures and to cultural background knowledge.
