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Earle's液与MEM培养基对神经元活性的影响 被引量:1
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作者 韩明 董丽萍 袁芳 《中国康复理论与实践》 CSCD 2005年第2期125-126,共2页
目的 观察Earle's液、新配最小必需培养基(MEM)及原 MEM对神经元活性的影响。方法 神经元培养 10 d后,分别换为Earle's液、新配MEM及原MEM,12 h和 24 h后用乳酸脱氢酶(LDH)试剂盒测定细胞的 LDH漏出量,四甲基偶氮唑盐(MTT)微量... 目的 观察Earle's液、新配最小必需培养基(MEM)及原 MEM对神经元活性的影响。方法 神经元培养 10 d后,分别换为Earle's液、新配MEM及原MEM,12 h和 24 h后用乳酸脱氢酶(LDH)试剂盒测定细胞的 LDH漏出量,四甲基偶氮唑盐(MTT)微量酶反应比色法测定MTT代谢率。结果 神经元在不同培养介质中孵育 12 h和 24 h后,细胞 LDH的漏出量及 MTT代谢率有非常显著性差异( P <0.01)。结论 不同孵育液对神经元的活性有影响。 展开更多
关键词 神经元 Earlés液 最小必需培养基(MEM) 乳酸脱氢酶 四甲基偶氮唑盐(MTT)
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追求利润——与Nick Earle谈电子商务发展的动力
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作者 禹刚 《互联网周刊》 2000年第19期29-29,共1页
5月8日惠普公司在北京举办了2000年HP电子化服务大世界的高峰论坛,这场主题为“洞悉网络明天、创新商业理念”的活动是北京第三界国际周的指定活动之一。惠普公司全球电子化服务解决方案总裁Nick Earle在这次高峰论坛中,作了“电子商务... 5月8日惠普公司在北京举办了2000年HP电子化服务大世界的高峰论坛,这场主题为“洞悉网络明天、创新商业理念”的活动是北京第三界国际周的指定活动之一。惠普公司全球电子化服务解决方案总裁Nick Earle在这次高峰论坛中,作了“电子商务中的电子服务”主题演讲,向参加这次活动的上千名业内人士介绍欧美等国电子商务发展状况,并对电子商务将来的发展提出了自己的看法。 展开更多
关键词 电子商务发展 Nick earle 利润 财政管理
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国内外虚拟参考服务实践及评价 被引量:7
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作者 牛学理 《情报科学》 CSSCI 北大核心 2003年第11期1221-1222,1229,共3页
文章对国外和国内有关数字参考服务的建设情况进行了简要的描述 ,做了一定的分析 ,并对我国进行数字参考服务提出建议。
关键词 中国 国外 虚拟参考服务 数字参考服务 网络咨询 EARL CDRS VRD 上海图书馆 知识导航
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饥饿条件下活性氧通过PINK1/Parkin通路调控人牙周膜细胞的线粒体自噬 被引量:3
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作者 范智博 金珂 +2 位作者 李胜鸿 徐洁 徐晓梅 《华西口腔医学杂志》 CAS CSCD 北大核心 2022年第6期645-653,共9页
目的探究人牙周膜细胞(hPDLC)在饥饿条件下活性氧(ROS)与PINK1/Parkin通路介导hPDLC线粒体自噬的具体机制。方法分离培养正常牙周组织的hPDLSC,利用Earle’s平衡盐溶液(EBSS)模拟饥饿环境诱导hPDLC线粒体自噬,利用N-乙酰-L-半胱氨酸(NAC... 目的探究人牙周膜细胞(hPDLC)在饥饿条件下活性氧(ROS)与PINK1/Parkin通路介导hPDLC线粒体自噬的具体机制。方法分离培养正常牙周组织的hPDLSC,利用Earle’s平衡盐溶液(EBSS)模拟饥饿环境诱导hPDLC线粒体自噬,利用N-乙酰-L-半胱氨酸(NAC)抑制ROS生成以探讨ROS在hPDLC线粒体自噬的作用,利用环孢素A(CsA)抑制PINK1/Parkin通路以研究ROS与PINK1/Parkin通路在饥饿条件下激活hPDLC中的作用。采用流式细胞术及JC-1线粒体膜电位检测试剂盒,检测线粒体膜电位;采用透射电镜观察线粒体自噬体的生成及线粒体形态变化;采用线粒体红色荧光探针定位线粒体,溶酶体绿色荧光探针定位溶酶体;采用DCFH-DA ROS荧光探针检测ROS生成强度;采用实时荧光定量聚合酶链反应(RT-qPCR)检测细胞中线粒体自噬基因(Tomm20、Timm23)及PINK1/Parkin通路的表达水平,采用蛋白质印迹(Western blot)检测细胞中线粒体自噬蛋白(Tomm20、Timm23)及PINK1/Parkin通路蛋白表达水平。结果EBSS饥饿作用30 min后,诱导激活hPDLC线粒体自噬的作用最强,ROS表达增加,且线粒体自噬相关基因(Tomm20、Timm23)下调(P<0.001),PINK1/Parkin通路表达上调(P<0.001)。NAC抑制ROS的产生后,自噬被抑制,同时Tomm20、Timm23表达上调(P<0.001,P<0.05),PINK1/Parkin通路表达下调(P<0.001,P<0.05)。而当CsA抑制PINK1/Parkin通路表达时(P<0.05,P<0.05),自噬被逆转,同时Tomm20、Timm23表达上调(P<0.001,P<0.01)。结论ROS在饥饿条件下主要通过PINK1/Parkin通路增强hPDLC线粒体自噬。 展开更多
关键词 earle’s平衡盐溶液 活性氧 线粒体自噬 人牙周膜细胞 PINK1/Parkin通路
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两种培养基对胚胎质量和IVF-ET妊娠率的影响 被引量:5
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作者 顾军 《中外医学研究》 2014年第27期37-38,共2页
目的:探讨采用earle’s培养液和HTF培养液两种培养基对胚胎质量和IVF-ET妊娠率的影响。方法:选取于笔者所在医院2013年期间进行的体外授精-胚胎移植技术治疗的患者34例(周期)。根据患者入院时间顺序进行分组,分别为A组和B组,A组受精卵... 目的:探讨采用earle’s培养液和HTF培养液两种培养基对胚胎质量和IVF-ET妊娠率的影响。方法:选取于笔者所在医院2013年期间进行的体外授精-胚胎移植技术治疗的患者34例(周期)。根据患者入院时间顺序进行分组,分别为A组和B组,A组受精卵子采用earle’s培养液,B组使用HTF培养液,每组研究对象17个周期。两组患者均采用常规体外授精-胚胎移植技术促排卵方案,密切观察卵子授精情况。比较两组的卵子授精率、卵裂率、移植周期妊娠率、优质胚胎率、种植率。结果:两组17个治疗周期中胚胎移植均为17个周期,两组卵裂率比较差异无统计学意义(P>0.05);earle’s培养液组的优质胚胎率、妊娠率、着床率均低于HTF培养液组(P<0.05)。结论:采用HTF培养液可明显提高优质胚胎率、妊娠率、着床率,且操作技术稳定,值得在临床中广泛推广。 展开更多
关键词 HTF培养基 earle’s培养基 胚胎质量 IVF-ET妊娠率
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Assessment of Economic Viability of Mini-livestock Production in Delta State,Nigeria:Implication for Extension Delivery Services
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作者 Ebewore Solomon Okeoghene Achoja Felix Odemero 《Journal of Northeast Agricultural University(English Edition)》 CAS 2016年第1期74-82,共9页
The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, ... The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, identify the types of animals reared as mini-livestock in the area, ascertain the reasons for keeping mini-livestock by farmers, determine the benefit level of mini- livestock farmers, and identify the problems facing mini-livestock farmers in the study area. Using snowball sampling technique, 674 respondents were sampled with the aid of a structured questionnaire. Various descriptive and inferential statistics were used for data analyses. The results of the study indicated various types of mini-livestock reared in the study area, mainly snail farming or heliciculture (about 90%) and rearing of cane rat (84.27%); the most important reasons for keeping mini-livestock were income (99.6%), employment (99.26%) and food (94.96%). The result further indicated that protein consumption and improved income were the most important benefits to respondents. In spite of these benefits, the respondents faced some constraints, amongst which were lack of technical information (X=4.70), lacking of enough space for expansion (X=4.58), inadequate training (X=4.20), poor extension delivery services (X=4.20) and problems of feed availability (X=3.86). In spite of these constraints, the production of mini-livestock was found to be profitable in the study area. Based on the t'mdings, it was recommended, among others, that mini-livestock farmers in the study area should be trained on various aspects of the management of mini-livestock especially by organising workshops for them. 展开更多
关键词 mini-livestock earle rat guinea pig VIABILITY SNAIL heliciculture
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中外会展场馆管理的比较研究——以中英会展场馆的个案为例 被引量:2
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作者 郑舒頔 《经济研究导刊》 2012年第26期53-56,共4页
以中国杭州白马湖国际会展中心和英国伦敦Earls Court会展中心作为中外会展场馆管理比较研究的对象,从物业管理、经营管理、场馆营销和品牌塑造等方面进行了对比分析,揭示了中国杭州白马湖国际会展中心的优势与不足,并就杭州白马湖国际... 以中国杭州白马湖国际会展中心和英国伦敦Earls Court会展中心作为中外会展场馆管理比较研究的对象,从物业管理、经营管理、场馆营销和品牌塑造等方面进行了对比分析,揭示了中国杭州白马湖国际会展中心的优势与不足,并就杭州白马湖国际会展中心的提升发展提出了若干建议。 展开更多
关键词 中外会展场馆管理 比较研究 杭州白马湖国际会展中心 英国伦敦Earls Court会展中心
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"Had He a Hand to Write This?" Missing "Secretary" Hands
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作者 W. Ron Hess Alan R. Tariea 《Journal of Literature and Art Studies》 2016年第10期1181-1202,共22页
When Elizabethan's were trained to read and write, the handwriting they first learned was "Secretary" (or "Secretarial") hand, reflecting the style used by monks and scribes well back into the Middle Ages. Only... When Elizabethan's were trained to read and write, the handwriting they first learned was "Secretary" (or "Secretarial") hand, reflecting the style used by monks and scribes well back into the Middle Ages. Only in the mid-1500s did an alternative hand called "Italic" (or "Italianate") slowly begin to be adopted as a second hand, reflecting handwriting used on the continent, and Italic was rarer than Secretary until well after 1600. Today, extant Elizabethan handwriting samples normally show each person used both hand styles, and where only one hand is extant for a given person, it is usually a Secretary hand. Thus, it's a surprise that two noblemen, the great William Cecil, Lord Burghley, and his son-in-law Edward DeVere, the 17th Earl of Oxford, are each assumed to have only Italic hand samples among their voluminous collections of handwriting. Did they not learn and never use Secretary hands, or is it that any Secretary hands in their documents are simply presumed to be written by clerks? This article begins with questioning whether the two men really were limited to Italic hands, fmds a few plausible Secretary hand samples for one of them (possibly for both), reconstructs a hypothetical Italic alphabet for him, and nominates many candidate manuscripts (MSS, singular MS) for having been written or contributed to by him, some of which may be relevant to Shakespeare studies. Other subjects touched on are calligraphy, a clerk (or amanuensis), and griffe de notaire (literally a "notary's scratch", or identifying scribble). 展开更多
关键词 Elizabethan handwriting-calligraphy William Cecil Lord Burghley Edward DeVere 17th Earl ofOxford SHAKESPEARE CALLIGRAPHY clerk-scribe-amanuensis griffe de notaire
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Comparing Two Latin Poetic Dedications by Anthony Munday and Robert Greene
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作者 W. Ron Hess 《Journal of Literature and Art Studies》 2016年第11期1310-1335,共26页
This article compares two Latin poem dedications which may contain hidden sub-meanings and possible displays of a basic puzzle method called acrostics, each dedicated to the same nobleman. The Latin verse in 1579 by A... This article compares two Latin poem dedications which may contain hidden sub-meanings and possible displays of a basic puzzle method called acrostics, each dedicated to the same nobleman. The Latin verse in 1579 by Anthony Munday follows acrostic English poems and may refer to his patron as "a lover of Pallas Athena" (the Spear-shaker of Greek mythology). The Latin verse in Robert Greene's 1584 book may contain a Latin acrostic and appears to identify Cupid, the "winged Love," as a companion of the same patron as Munday's from five years earlier. That Cupid trope appears similar to "the little love god" allusions to Cupid in several of Shakespeare's sonnets (e.g., #s 153 and 154). More than these discussions, this article explores difficulties of translating from Latin into English, or vice versa, and the pitfalls which can occur. 展开更多
关键词 Latin Translations Anthony Munday Robert Greene Edward DeVere 17th Earl of Oxford Shakespeare's Sonnets
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In memoriam of Thomas Earl Starzl,the pioneer of liver transplantation
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作者 Sezai Yilmaz Sami Akbulut 《World Journal of Transplantation》 2022年第3期55-58,共4页
Starzl’s nearly 3000 publications that contribute to the science of transplantation in every field have been the most important resources for every scientist working in this field.For those of us who work in the live... Starzl’s nearly 3000 publications that contribute to the science of transplantation in every field have been the most important resources for every scientist working in this field.For those of us who work in the liver transplant field,his contributions throughout his life have shaped our career and passion,even for those who have never met,spoken to,or worked with him.If we are able to help patients with liver failure today by offering them the chance of transplantation,it is because of Starzl’s passionate work and efforts.Thanks to Starzl’s scientific legacy,hundreds of scientists serve humanity and thousands of patients can hold on to life.It has been an honor for us to write this article about Professor Starzl. 展开更多
关键词 Liver transplantation Thomas Earl Starzl Pioneer of liver transplantation
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Embryonic intrauterine growth restriction: report of two cases
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作者 李小毛 沈慧敏 史众杰 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第11期1753-1755,共3页
关键词 EMBRYO intrauterine growth restriction earl y pregnancy
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H^∞极大理想空间上的Earl定理
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作者 郭坤宇 《复旦学报(自然科学版)》 CAS CSCD 北大核心 1996年第4期393-396,共4页
在这篇文章中,使用函数空间理论的技巧,证明了H∞极大理想空问上的Earl定理,这个定理也许在函数空间插值理论中有重要应用.
关键词 Earl定理 插值Blaschke积 零集 极大理想空间
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TYPHOON AND RAINSTORM EARLY WARNING AND ASSESSING SYSTEM PUT IN REAL-TIME TRIAL OPERATION
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作者 Li Huiming 《Natural Disaster Reduction in China》 1996年第1期45-46,共2页
TYPHOONANDRAINSTORMEARLYWARNINGANDASSESSINGSYSTEMPUTINREAL-TIMETRIALOPERATIONLiHuimingAsacomponentofsubject0... TYPHOONANDRAINSTORMEARLYWARNINGANDASSESSINGSYSTEMPUTINREAL-TIMETRIALOPERATIONLiHuimingAsacomponentofsubject09ofthekeyscientif... 展开更多
关键词 TRIA EARL
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THE EARLY FROST CHARACTERISTICS AND ITS PREVENTIVE AND CURATIVE STRATEGY FOR FRUIT TREES IN YUCHENG,SHANDONG PROVINCE
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作者 Su Peixi (Lanzhou Institute of Desert, Chinese Academy of Sciences ) 《Natural Disaster Reduction in China》 1996年第2期85-87,共3页
THEEARLYFROSTCHARACTERISTICSANDITSPREVENTIVEANDCURATIVESTRATEGYFORFRUITTREESINYUCHENG,SHANDONGPROVINCESuPeix... THEEARLYFROSTCHARACTERISTICSANDITSPREVENTIVEANDCURATIVESTRATEGYFORFRUITTREESINYUCHENG,SHANDONGPROVINCESuPeixi(LanzhouInstitut... 展开更多
关键词 EARL
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Leshan Sees Growth in Polysilicon Industry
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《China Chemical Reporter》 2009年第23期10-10,共6页
Leshan, a city in southwest China’sSichuan province, attempts to create threeindustrial chains for silicon materials usedin photovoltaic cells, electronic industry,and silicon chemicals recycle utilization.The electr... Leshan, a city in southwest China’sSichuan province, attempts to create threeindustrial chains for silicon materials usedin photovoltaic cells, electronic industry,and silicon chemicals recycle utilization.The electronic and silicon materials busi-nesses contributed RMB6.96 billion 展开更多
关键词 CHEMICALS photovoltaic CHAINS RECYCLE REVENUE earl
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Sinopec Corp.Announces 2009 Interim Results
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《China Chemical Reporter》 2009年第25期7-7,共6页
China Petroleum & Chemical Corporation(Sinopec Corp., HK: 386; NYSE: SNP; LSE:SNP; SH: 600028) announces its interim re-sults for year ended June 30th, 2009.In accordance with the PRC AccountingStandards for Busin... China Petroleum & Chemical Corporation(Sinopec Corp., HK: 386; NYSE: SNP; LSE:SNP; SH: 600028) announces its interim re-sults for year ended June 30th, 2009.In accordance with the PRC AccountingStandards for Business Enterprises, thecompany’s turnover was RMB534.025billion, down 26.9% from the same period in2008. Net profit was RMB33.19 billion, rep-resenting an increase of 332.6% over the sameperiod of previous year. EPS was RMB0.383, up 332.6% year-on-year.In accordance with the International Finan-cial Reporting Standards (IFRS), thecompany’s turnover and other operating rev-enues amounted to RMB534.025 billion, 展开更多
关键词 PETROLEUM TURNOVER PROFIT accordance thousand earl
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Prices of Organics Changed Differently
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《China Chemical Reporter》 2009年第21期18-18,共6页
PhenolIn June 2009, China’s phenol prices firstlyrose apparently with increased trading actionsbecause of the price rise of crude oilin the international market and then fellslightly in response to the high inventory... PhenolIn June 2009, China’s phenol prices firstlyrose apparently with increased trading actionsbecause of the price rise of crude oilin the international market and then fellslightly in response to the high inventory.As of June 30th, the bulk trading pricesof phenol were RMB6 900-7 000/t at 展开更多
关键词 TRADING apparently PHENOL PRICES CRUDE PHENOL earl
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