A total of 12 mature healthy Holstein dairy cows of the nearly body weight （580±30） kg, milk yield （22.5±2.8） kg in the early stages of lactation were selected in this experiment. The cows were randomly ...A total of 12 mature healthy Holstein dairy cows of the nearly body weight （580±30） kg, milk yield （22.5±2.8） kg in the early stages of lactation were selected in this experiment. The cows were randomly divided into 2 groups, every group had 6 cows, every group had 6 repeats, and every repeat had 1 cow. Added 20 g protected methionine in earlier lactating cow food every day. The results showed that protected methionine increased milk yield by 10.83%, testing group milk yield was significantly different than that of control （P〈0.05）; protected methionine increased milk fat by 5.98%, testing group milk fat was significantly different than that of control （P〈0.05）; Milk protein increased by 2.15%, but bad insignificantly different （P〉0.05）; dry matter of milk had the tendency of decrease, but had insignificant difference （P〉0.05）.展开更多
The fire distribution can be divided into weapon assignment and firing time scheduling. The criterion of weapon allocation is that a target with greater threat has higher priority. And the criterion of firing time sch...The fire distribution can be divided into weapon assignment and firing time scheduling. The criterion of weapon allocation is that a target with greater threat has higher priority. And the criterion of firing time scheduling is that a target can be damaged with the expected probability before a specific time. A fire distribution scheme and a program for the integrated missile-gun air defense system based on a criterion of earlier damage were presented. An example was taken to illustrate its effectiveness.展开更多
Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis...Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that control visual cortex development.展开更多
基金National Key Research Program of China (2006BAD04A03-02)Heilongjiang Province Office of Education Funded Major Projects (10541z003)Dr. Start-up Funds of Northeast Agricultural University
文摘A total of 12 mature healthy Holstein dairy cows of the nearly body weight （580±30） kg, milk yield （22.5±2.8） kg in the early stages of lactation were selected in this experiment. The cows were randomly divided into 2 groups, every group had 6 cows, every group had 6 repeats, and every repeat had 1 cow. Added 20 g protected methionine in earlier lactating cow food every day. The results showed that protected methionine increased milk yield by 10.83%, testing group milk yield was significantly different than that of control （P〈0.05）; protected methionine increased milk fat by 5.98%, testing group milk fat was significantly different than that of control （P〈0.05）; Milk protein increased by 2.15%, but bad insignificantly different （P〉0.05）; dry matter of milk had the tendency of decrease, but had insignificant difference （P〉0.05）.
基金Sponsored by Jiangsu Planned Project for Postdoctoral (0901014B)
文摘The fire distribution can be divided into weapon assignment and firing time scheduling. The criterion of weapon allocation is that a target with greater threat has higher priority. And the criterion of firing time scheduling is that a target can be damaged with the expected probability before a specific time. A fire distribution scheme and a program for the integrated missile-gun air defense system based on a criterion of earlier damage were presented. An example was taken to illustrate its effectiveness.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 81070765） and the Department of Science ＆ Technology of Guangdong Province, China （No. 2004A30801001）
文摘Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that control visual cortex development.
