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EuSHT Acts as a Hub Gene Involved in the Biosynthesis of 6-Hydroxyluteolin and Quercetin Induced by Salt Stress in Eucommia ulmoides
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作者 Fuxin Li Enyan Chen +5 位作者 Xinxin Chen Jingyu Jia Hemin Wang Jie Zhang Jianrui Sun Xin Li 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第8期2095-2113,共19页
Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites.Flavonoids are natural compounds that possess many important biological activities,playing a significant role in the medicina... Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites.Flavonoids are natural compounds that possess many important biological activities,playing a significant role in the medicinal activity of Eucommia ulmoides(E.ulmoides).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in E.ulmoides,a comprehensive analysis of metabolomics and transcriptomics was conducted.The results indicated that salt stress led to the wilting and darkening of E.ulmoides leaves,accompanied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical conductivity.During salt stress,most metabolites in the flavonoid biosynthesis pathway of E.ulmoides were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt stress.Among them,secondary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt stress.The correlation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as 6-Hydroxyluteolin.The co-expression network showed a strong positive correlation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative correlation with Catechin biosynthesis.The branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid compound biosynthesis in the plant stress response during salt stress.The results of this study provided a preliminary mechanism of secondary metabolites such as flavonoids in the medicinal plant E.ulmoides induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress response.It also provided useful information for subsequent exploration of resistance genes in E.ulmoides. 展开更多
关键词 eucommia ulmoides flavonoid salt stress TRANSCRIPTOMICS widely targeted metabolomics
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Comprehensive Assessment of the Safety of Eucommia ulmoides Leaf Extract for Consumption as a Traditional Chinese Health Food 被引量:2
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作者 Huiling Fu Mijun Peng +4 位作者 Qiuwen Tang Haojun Liang Yanli Liang Jiali Fang Xuesong Wang 《Journal of Renewable Materials》 EI 2023年第7期3091-3114,共24页
To ensure the export quality of Eucommia ulmoides leaf extract(ELE)and facilitate E.ulmoides leaf inclusion in the directory of traditional Chinese health foods,an overall safety assessment of ELE was performed,includ... To ensure the export quality of Eucommia ulmoides leaf extract(ELE)and facilitate E.ulmoides leaf inclusion in the directory of traditional Chinese health foods,an overall safety assessment of ELE was performed,including genotoxicity and long-term toxicity,according to the national food safety standards of China.No variations in the reverse mutation number of the nominal bacterial strains were observed under ELE treatment in comparison with the solvent control.Additionally,the micronucleus rates of in vivo mammalian erythrocytes and in vitro mammalian cells under ELE treatment were equivalent to or significantly lower than those of the solvent control.The fold change in the trifluorothymidine resistance mutation frequency of the thymidine kinase gene under ELE treatment was less than three times in comparison with the solvent control,suggesting that ELE did not cause genotoxicity.Moreover,animal experiments showed that the growth performance of rats under ELE treatment was enhanced because the body weights of rats increased.No oxidative injury or inflammatory responses were induced and no histopathological lesions of tissues were detected under ELE treatment.In addition,plasma triglycerides and low-density lipoprotein cholesterol levels significantly decreased,and plasma high-density lipoprotein cholesterol levels significantly increased with ELE treatment,suggesting that ELE was health-promoting.Furthermore,moderate to excellent antimicrobial activities,a favorable anticancer capacity,and superior antioxidant abilities of ELE were found,implying ELE possesses good bioactivities.Therefore,we affirmed ELE is safe to consume as a traditional Chinese health food. 展开更多
关键词 eucommia ulmoides leaf extract GENOTOXICITY long-term toxicity growth performance bioactivity
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Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides
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作者 Jiali Wang Xiangmei Chen +2 位作者 Xiaozhen Huang Yichen Zhao Degang Zhao 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第5期1377-1389,共13页
The phytoene desaturase(PDS)encodes a crucial enzyme in the carotenoid biosynthesis pathway.Silencing or inhibiting PDS expression leads to the appearance of mottled,chlorosis,or albino leaves.In this study,the CDS se... The phytoene desaturase(PDS)encodes a crucial enzyme in the carotenoid biosynthesis pathway.Silencing or inhibiting PDS expression leads to the appearance of mottled,chlorosis,or albino leaves.In this study,the CDS sequence of EuPDS(Eucommia ulmoides Phytoene Desaturase)was first cloned and then PDS was silenced in Nicotiana benthamiana.Result showed the expression level of EuPDS in leaves was higher than that in the roots and stems.In N.benthamiana leaves,which were treated by Agrobacterium for 24 h,photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the period of emersion.This suggested that EuPDS could silence PDS of N.benthamiana,so as to cause the phenotype of leaf whitening.PDS is the main reporter gene involved in virus-induced gene silencing(VIGS).This study offered molecular evidence for identifying PDS gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in E.ulmides.It also laid a useful foundation for study on leaf discoloration mechanism of other woody plants. 展开更多
关键词 eucommia ulmoides EuPDS virus-induced gene silencing TRV relative expression
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Overexpression ofβ-1,4-Glucanase Gene EuEG1 Improves Micrografting of Eucommia ulmoides
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作者 Lei Wang Runying Wang +2 位作者 Yi Li Yichen Zhao Degang Zhao 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第11期3063-3075,共13页
Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia ulmoides.Micrografting offers a new method for the transplantation of genetic transformation,and its su... Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia ulmoides.Micrografting offers a new method for the transplantation of genetic transformation,and its success depends on the formation of graft unions.This study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing process.We found that the EuEG1 gene is a vital regulator of graft,and its overexpression contributes to the survival of Eucommia ulmoides micrografting.The EuEG1 gene transgenic plants(TP)used as scions for micrografting presented a significantly higher survival rate than the wild type(WT)and empty vector(EV)regenerated scions.During the grafting healing process,the expression of the EuEG1 gene was higher during the period of callus proliferation,suggesting that the EuEG1 gene was involved in the graft healing process.Histological observation revealed that more calluses tissue appeared at the junction of transgenic scions,and the connection with the rootstock was stronger,which benefits wound healing.These results provide new insights into Eucommia ulmoides micrografting and indicate that the EuEG1 gene can promote wound healing and improve the micrografting survival rate. 展开更多
关键词 eucommia ulmoides genetic transformation EuEG1 gene MICROGRAFTING
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Role and Effects of Eucommia ulmoides Leaves in the Growth Process of Livestock and Poultry
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作者 Shijin GUO Ling LI +6 位作者 Xin LI Yi ZHANG Jianjun WANG Lei ZHAO Yanping WANG Lu GUO Shijun FU 《Agricultural Biotechnology》 CAS 2023年第5期43-45,共3页
Substitutes of feed antibiotics have been a key research topic in the new stage of animal husbandry.Chinese veterinary medicine refers to decoction pieces and their preparations processed from natural plants,animals,a... Substitutes of feed antibiotics have been a key research topic in the new stage of animal husbandry.Chinese veterinary medicine refers to decoction pieces and their preparations processed from natural plants,animals,and minerals and used for animal disease prevention and improvement of animal production performance under the guidance of Chinese veterinary pharmacy theory.Eucommia ulmoides leaf extract has many active functional components such as chlorogenic acid,E.ulmoides polysaccharides and flavonoids,which have many biological properties such as antibacterial,antioxidant,immune-regulation,sugar ester-and bone metabolism-regulation effects.This paper explored active ingredients and biological properties of E.ulmoides leaf extract,as well as its role and effects in livestock and poultry breeding,providing a scientific basis for the use of E.ulmoides leaves in livestock and poultry breeding. 展开更多
关键词 eucommia ulmoides leaves Livestock and poultry farming ROLE EFFICACY
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Dynamic Changes in Distribution of Lignin and Hemicelluloses in Cell Walls During Differentiation of Secondary Xylem in Eucommia ulmoides 被引量:5
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作者 贺新强 崔克明 李正理 《Acta Botanica Sinica》 CSCD 2001年第9期899-904,共6页
The dynamic changes in the distribution of lignin and hemicelluloses (xylans and xyloglucans) in cell walls during the differentiation of secondary xylem in Eucommia ulmoides Oliv. were studied by means of ultraviolet... The dynamic changes in the distribution of lignin and hemicelluloses (xylans and xyloglucans) in cell walls during the differentiation of secondary xylem in Eucommia ulmoides Oliv. were studied by means of ultraviolet light microscopy and transmission electron microscopy combined with immunogold labelling. In the cambial zone and cell expansion zone, xyloglucans were localized both in the tangential and radial walls, but no xylans or lignin were found in these regions. With the formation of secondary wall S-1 layer, lignin occurred in the cell corners and middle lamella, while xylans appeared in S-1 layer, and xyloglucans were localized in the primary walls and middle lamella. In pace with the formation of secondary wall S-2 and S-3 layer, lignification extended to S-1, S-2 and S-3 layer in sequence, showing a patchy style of lignin deposition. Concurrently, xylans distributed in the whole secondary walls and xyloglucans, on the other hand, still localized in the primary walls and middle lamella. The results indicated that along with the formation and lignification of the secondary wall, great changes had taken place in the cell walls. Different parts of cell walls, such as cell corners, middle lamella, primary walls and various layers of secondary walls, had different kinds of hemicelluloses, which formed various cell wall architecture combined with lignin and other cell wall components. 展开更多
关键词 cell wall LIGNIN hemicelluloses secondary xylem differentiation eucommia ulmoides
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Ultracytochemical Localization of ATPase During the Secondary Xylem Differentiation and Dedifferentiation in Eucommia ulmoides Trunk 被引量:12
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作者 王雅清 Kalima-N’ +1 位作者 KomaMWANGE 崔克明 《Acta Botanica Sinica》 CSCD 2000年第5期455-460,共6页
The ultracytochemical localization of ATPase in the secondary xylem cells during their differentiation and dedifferentiation in the girdled Eucommia ulmoides Oliv. was carried out using a lead phosphate precipitation ... The ultracytochemical localization of ATPase in the secondary xylem cells during their differentiation and dedifferentiation in the girdled Eucommia ulmoides Oliv. was carried out using a lead phosphate precipitation technique. Throughout the differentiation, which is a typical programmed cell death (PCD) process, ATPase deposits increased in the nucleus but decreased and progressively disappeared in the cell organelles. At the same time, the distribution of ATPase increased in the inner face of the cell wall and pits with cytoplasmic degeneration. The results demonstrated that the PCD was an energy dependent active process and was controlled by nuclear genes. On the other hand, the distribution of ATPase in the intercellular spaces increased with the formation of the new cambium resulted from the dedifferentiation of the secondary xylem cells after girdling. However, ATPase was not found in the nucleus of the dividing cells, suggesting that nutrients were transported through protoplast during differentiation, and through both protoplast and apoplast during dedifferentiation. Thus, the energy required in cell division was provided mainly by intercellular spaces. These findings indicate that the dynamic distribution of ATPase reflected which cell component was actively taking part in the cell metabolism at various stages of the plant development, and its distribution was associated with the physiological state of the cell. Based on the characteristic distributions of ATPase, the critical stage of cell differentiation and the relationship between the critical stage and dedifferentiation were discussed. 展开更多
关键词 eucommia ulmoides secondary xylem DIFFERENTIATION DEDIFFERENTIATION programmed cell death (PCD) ATPASE
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Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides 被引量:2
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作者 曹静 贺新强 +2 位作者 王雅清 苏都莫日根 崔克明 《Acta Botanica Sinica》 CSCD 2003年第12期1465-1474,共10页
Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases ... Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gel electrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-end labeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-like proteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation. The results thus indicated that secondary xylem differentiation in E ulmoides was a typical process of PCD and the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation. 展开更多
关键词 caspase-like protease DNA fragmentation eucommia ulmoides poly (ADP-ribose) polymerase programmed cell death secondary xylem differentiation
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Effect of Regeneration After Girdling on Tree Growth in Eucommia ulmoides 被引量:5
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作者 崔克明 李正理 《Acta Botanica Sinica》 CSCD 2000年第11期1115-1121,共7页
Annual trunk diameters and tree heights were measured in July after initial_girdling and regirdling in Eucommia ulmoides Oliv. And the percentages of fallen leaves in all leaves and burst buds in all buds were est... Annual trunk diameters and tree heights were measured in July after initial_girdling and regirdling in Eucommia ulmoides Oliv. And the percentages of fallen leaves in all leaves and burst buds in all buds were estimated respectively on every autumn or spring. The results indicated that although the tree growth slowed down in the first year after girdling, but in the second year after girdling, the growth of the girdled trees tended to become normalized and up to the third year, it was similar to that of the normal trees. According to its developmental trait after girdling, the authors suggested that optimal girdling of the trees is in every 4 or 5 year intervals. With this method it was able to improve bark production and to protect the resource from extinction as well. 展开更多
关键词 eucommia ulmoides GIRDLING REGENERATION growth\
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Ultrastructural Observation of Changes of Polysaccharide Grains in Dormant Shoots of Eucommia ulmoides 被引量:4
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作者 崔克明 罗立新 李正理 《Acta Botanica Sinica》 CSCD 2000年第8期788-793,共6页
Cytochemical and ultrastructural studies indicated that compound spherical bodies observed near the sieve plate in the sieve tube members during the period of cambial dormancy in the shoots of Eucommia ulmoides O... Cytochemical and ultrastructural studies indicated that compound spherical bodies observed near the sieve plate in the sieve tube members during the period of cambial dormancy in the shoots of Eucommia ulmoides Oliv. were polysaccharide bodies different from the polysaccharide grains in other parenchyma cells in shoots, and were similar to cell walls in their staining properties. The compound spherical bodies occurred in the sieve elements during the period of cambial rest and disappeared in the sieve elements during the period of cambial quiescence and activity. 展开更多
关键词 ULTRASTRUCTURE polysaccharide grains sieve tube member DORMANCY eucommia ulmoides
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Mechanism of Dormancy in the Buds and Cambium of Eucommia ulmoides 被引量:2
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作者 Kalima N’Koma MWANGE 王学文 崔克明 《Acta Botanica Sinica》 CSCD 2003年第6期698-704,共7页
This paper describes changes in the structure of buds and vascular cambium as well as in protein content of one-year-old dormant branches of Eucommia ulmoides Oliv. in water-culture conditions. Results confirm the exi... This paper describes changes in the structure of buds and vascular cambium as well as in protein content of one-year-old dormant branches of Eucommia ulmoides Oliv. in water-culture conditions. Results confirm the existence of 2 quiescences (Q1, Q2) and 1 rest (R) phases in this tree during the dormancy period. In the R time, the E. ulmoides cambium was unable to reactivate even though the tree was subjected to exogenous IAA, suitable temperature or required luminosity. Furthermore, pistillate trees entered the dormant phase earlier than staminate ones. The protein content in the bark during Q1 increased significantly ( P < 0.01), but drastically decreased in the R period, before rising up again at the onset of Q2 ( P < 0.01). Even though the change pattern of protein content was similar, its occurrence is much earlier in staminate than in pistillate plants. SDS-PAGE revealed a 'special' protein band of 11.8 kD in the transitional Q1-R-Q2 stages. This 'special' protein bands would be related to the cambial dormancy and to the regulation of the Q1-R-Q2 transition. 展开更多
关键词 eucommia ulmoides IAA QUIESCENCE REST special protein
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Comparative analysis of chemical components between barks and leaves of Eucommia ulmoides Oliver 被引量:10
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作者 周菊峰 张泰铭 +1 位作者 陈望爱 梁逸曾 《Journal of Central South University》 SCIE EI CAS 2009年第3期371-379,共9页
The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), a... The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%-106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones. 展开更多
关键词 couple chromatography chemometric algorithm comparative analysis essential oil FLAVONE eucommia ulmoides Oliver
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Chemical constituents,biological functions and pharmacological effects for comprehensive utilization of Eucommia ulmoides Oliver 被引量:22
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作者 Yi-Fan Xing Dong He +6 位作者 Yi Wang Wen Zeng Chong Zhang Yuan Lu Nan Su Yan-Hua Kong Xin-Hui Xing 《Food Science and Human Wellness》 SCIE 2019年第2期177-188,共12页
Eucommia ulmoides Oliver is a native plant and valuable tonic Chinese medicine in China with a long history,great economic value and comprehensive development potential.Traditionally,the comprehensive utilization rate... Eucommia ulmoides Oliver is a native plant and valuable tonic Chinese medicine in China with a long history,great economic value and comprehensive development potential.Traditionally,the comprehensive utilization rate of E.ulmoides Oliv.is still very low,only bark has been used as medicine and other parts of Eucommia ulmoides Oliv.cannot be fully utilized,even the leaves have been well utilized in food products in Japan in the past decades.In order to improve the comprehensive utilization efficiency of E.ulmoides Oliv.,in this review,we summarized the varieties and contents of main active compounds,biological functions and pharmacological effects in different parts of E.ulmoides Oliv.The findings suggest that other parts of E.ulmoides Oliv.could replace the bark of E.ulmoides Oliv.to some extent besides of their respective applications.The unique and extensive physiological functions between different parts of E.ulmoides Oliv.indicate that the comprehensive utilization of E.ulmoides Oliv.has a wide space to develop,which is also an effective way to protect E.ulmoides Oliv.resources and improve its the utilization rate. 展开更多
关键词 eucommia ulmoides Oliver Comprehensive utilization Chemical constituents Biological functions Pharmacological effects
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Isolation of a novel antifungal peptide from the bark of Eucommia ulmoides Oliv 被引量:2
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作者 刘世会 Zhao Degang Han Yuzhen 《High Technology Letters》 EI CAS 2008年第2期216-219,共4页
A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3... A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3 is 4157.3 Da, and its partial amino acid sequence is -. LYQQLIAGITLNK.-. EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration required for 50% growth inhibition (IC50) is 31.25μg/mL. 展开更多
关键词 antifungal peptide ISOLATION the bark of eucommia ulmoides Oliv Candida albicans
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Determining the in vitro Anti-Aging Effect of the Characteristic Components from Eucommia ulmoides 被引量:1
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作者 Xuesong Wang Zhihong Wang +2 位作者 Qiuling Yang Sheng Peng Mijun Peng 《Journal of Renewable Materials》 SCIE EI 2022年第12期3131-3145,共15页
To evaluate the potential anti-aging ability of Eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidativ... To evaluate the potential anti-aging ability of Eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidative damage model of human umbilical vein endothelial cell(HUVEC).Oxidative damage indexes,inflammatory factors,cell cycle,cell apoptosis,cell senescence,and their related proteins were analyzed by methyl thiazolyl tetrazolium(MTT)assay,enzyme-linked immunosorbent assay(ELISA),propidium iodide(PI)staining,annexin V-FITC/PI double staining,SAβ-galactosidase staining,and western blotting(WB).The results showed that H_(2)O_(2)-induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H_(2)O_(2) group.Meanwhile,the contents of antioxidant indexes(reactive oxygen species,lactate dehydrogenase,molondialdehyde,superoxide dismutase,glutathione),inflammatory factors(nuclear factor kappa-B,intercellular cell adhesion molecule-1,vascular cell adhesion protein 1),and functional factors(NO,Endothelin-1)in characteristic components treated groups improved if comparison with H_(2)O_(2) group,suggesting the characteristic components of E.ulmoides could alleviate H_(2)O_(2)-induced oxidative damage.Moreover,cell cycle,cell apoptosis,cell senescence,and their related proteins under characteristic components treatment exhibited a better effect than under H_(2)O_(2) treatment,implying the characteristic components could participate in anti-aging via multiple pathways.These results manifested that the characteristic components of E.ulmoides posses the capacity of anti-aging,which provided a basis for investigating the anti-aging ability of E.ulmoides itself. 展开更多
关键词 ANTI-AGING eucommia ulmoides characteristic components oxidative damage cell senescence
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Transcriptomic and Physiological Analyses of Pigment Accumulation in Eucommia ulmoides‘Hongye’
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作者 Mengjiao Chen Jinhui Zhai +6 位作者 Jiajia Zhang Hui Li Xinjiang Niu Yaxin Liu Yue Ren Hongyan Du Jingle Zhu 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第5期1027-1044,共18页
Eucommia ulmoides‘Hongye’is a new ornamental variety of E.ulmoides with excellent red or purple foliage.We found that E.ulmoides‘Hongye’exhibited a gradual change from green to red colour under light conditions.Ho... Eucommia ulmoides‘Hongye’is a new ornamental variety of E.ulmoides with excellent red or purple foliage.We found that E.ulmoides‘Hongye’exhibited a gradual change from green to red colour under light conditions.However,the colouring mechanism in the leaves of E.ulmoides‘Hongye’remains unclear.In this study,we compared the pigment content and leaf colour index of E.ulmoides‘Hongye’at five stages with those of E.ulmoides‘Xiaoye’,which was used as the control variety.The transcriptome sequencing data of the first-period(H1,green)and fifth-period(H5,red)leaves were also analysed and compared.The corresponding gene regulation in anthocyanin-related metabolic pathways was then analysed.Physiological results indicated that the contents of flavonoids and anthocyanins in red leaves(H5)were significantly higher than those in green leaves(H1),whereas the chlorophyll content in red leaves(H5)was lower than that in green leaves(H1).Moreover,the carotenoid content did not significantly differ between the two varieties.A transcriptome analysis identified 4240 differentially expressed genes(DEGs),and 20 of these genes were found to be involved in flavonoid and anthocyanin biosynthesis pathways.The results provide a reference for further study of the leaf colouration mechanism in E.ulmoides. 展开更多
关键词 eucommia ulmoides leaf colouring ANTHOCYANIN TRANSCRIPTOMICS flavonoid biosynthesis
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Cloning and Characterization of EuGID1 in Eucommia ulmoides Oliver
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作者 Yulu Chen Fen Zhang +3 位作者 Ju Cai Yichen Zhao Jiaxue Cui Yan Li 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第5期999-1013,共15页
Gibberellic acid controlled the key developmental processes of the life cycle of landing plants,and regulated the growth and development of plants.In this study,a novel gibberellin receptor gene EuGID1 was obtained fr... Gibberellic acid controlled the key developmental processes of the life cycle of landing plants,and regulated the growth and development of plants.In this study,a novel gibberellin receptor gene EuGID1 was obtained from Eucommia ulmoides Oliver.The cDNA of EuGID1 was 1556 bp,and the open reading frame was 1029 bp,which encoded 343 amino acids.EuGID1 had the homology sequence with the hormone-sensitive lipase family.Amino acid sequence alignment confirmed EuGID1 protein had the highest homology with the GID1 protein of Manihot esculenta.EuGID1 was located in the nucleus and cell membrane and had expression in four plant organs.Overexpression of EuGID1 in transgenic Arabidopsis plants promoted plant elongation and increased siliques yield. 展开更多
关键词 eucommia ulmoides Oliver EuGID1 gene OVEREXPRESSION subcellular localization heterologous transformation
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Novel Green resource material:Eucommia Ulmoides Gum
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作者 Donghan Li Chen Yang +5 位作者 Yiqi Huang Long Li Wenchi Han Hailan Kang Feng Yang Qinghong Fang 《Resources Chemicals and Materials》 2022年第1期114-128,共15页
Eucommia ulmoides gum(EUG),main composition is trans-1,4-polyisoprene,is a natural polymer extracted from Eucommia ulmoides plant tissue.Benefiting from the crystallization ability and rubber-plastic duality,it can be... Eucommia ulmoides gum(EUG),main composition is trans-1,4-polyisoprene,is a natural polymer extracted from Eucommia ulmoides plant tissue.Benefiting from the crystallization ability and rubber-plastic duality,it can be applied to a variety of fields,including aerospace,national defense,healthcare,transportation,sports,and con-struction.Herein,we summarized recent progress in EUG research concerning efficient extraction methods,crys-tallization characteristics and novel functional EUG materials focused on the relationship between its molecular structure,crystallization behavior,phase structure,and properties.Furthermore,the research and development directions of EUG for the development of its new materials have been outlined. 展开更多
关键词 eucommia ulmoides gum Extraction Crystallization Modification Functional material
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Dietary Eucommia ulmoides leaf extract improves laying performance by altering serum metabolic profiles and gut bacteria in aged laying hens 被引量:1
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作者 Xiaoxiao Liang Yawei Fu +4 位作者 Kaimin Niu Zhenya Zhai Hongxun Shi Ruxia Wang Yulong Yin 《Animal Nutrition》 SCIE CAS CSCD 2023年第4期307-319,共13页
The leaves of Eucommia ulmoides are rich in bioactive constituents that have potential gastrointestinal benefits for animals.In aged laying hens,intestinal health issues contribute to a significant decline in egg-layi... The leaves of Eucommia ulmoides are rich in bioactive constituents that have potential gastrointestinal benefits for animals.In aged laying hens,intestinal health issues contribute to a significant decline in egg-laying capacity during intermediate and later stages.It remains unclear whether E.ulmoides leaf extract(ELE)can improve intestinal health and enhance egg production in elderly laying hens,and the underlying mechanisms are yet to be elucidated.Therefore,we conducted a study with 480 laying hens(65 weeks old)randomly allocated into four groups:a control group fed with the basal diet,and three treatment groups supplemented with 500,1,000,and 2,000 mg/kg of ELE,respectively.The primary active constituents of ELE include flavonoids,polysaccharides,terpenoids,and phenolic acids.Dietary supplementation with ELE at 1,000 mg/kg(ELE1000)significantly improved laying performance and egg quality compared to the other groups.ELE1000 stimulated the maturation of intestinal epithelial cells,increased villus height,and reduced crypt depth.It also influenced the levels of proteins associated with tight junctions(claudin-1 and claudin-2)and intestinal inflammatory factors(IL-6,IL-1β,and IL-2)in different intestinal sections.Integrative analysis of serum metabolomics and gut microbiota revealed that ELE1000 improved nutrient metabolism by modulating amino acid and ubiquinone biosynthesis and influenced the abundance of intestinal microbiota by enriching pivotal genera such as Bacteroides and Rikenellaceae_RC9_gut_group.We identified 15 metabolites significantly correlated with both gut microbiota and laying performance,e.g.,DL-methionine sulfoxide,THJ2201 N-valerate metabolite,tetracarbonic acid,etc.In conclusion,ELE1000 improved laying performance in elderly laying hens by affecting intestinal morphology,barrier function,microbiota,and serum metabolite profiles.These findings suggest that ELE can be a beneficial feed additive for extending the peak producing period in aged laying hens. 展开更多
关键词 eucommia ulmoides leaf extract Laying performance Aged laying hens Gut microbiota Serum metabolomics
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Pharmacokinetic study about compatibility of Eucommia ulmoides and Psoralea corylifolia
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作者 Yuxing Huang Erwei Liu +3 位作者 Xuhua Huang Jia Hao Siyuan Hu Xiumei Gao 《Chinese Herbal Medicines》 CAS 2023年第2期263-270,共8页
Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat was explored in this study.Methods:Eu extract,Pc extract and the combined extracts(crude ... Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat was explored in this study.Methods:Eu extract,Pc extract and the combined extracts(crude drug ratio was 2:1)was administered by gavage,respectively.Two PK experiments were conducted.In first one,the blood samples were collected via the occuli chorioideae vein to get the PK properties of the components.In second one,the blood samples were simultaneously collected via the internal jugular vein or portal vein at different time points and the concentrations of target ingredients were detected by LC/MS/MS to clear the location where the interaction of Eu and Pc took place in vivo.Results:Eight of 11 ingredients in Eu and Pc extract were determined in rat plasma.The exposure levels of geniposidic acid(GPA),aucubin(AU),geniposide(GP),pinoresinol diglucoside(PDG),psoralen glycosides(PLG)and isopsoralen glycosides(IPLG)were decreased 1/5–2/3 after administration of combined extracts.Comparing to the combined administration,the exposure of GPA and AU in plasma of single Eu administration collected via the portal vein were decreased 1/3–2/3,and the values of AUC0-24h and AUC0-∞ of GP collected from the portal vein or internal jugular vein were double increased.The other components’parameters were not significantly changed.Conclusion:In summary,the Pc and Eu combined administration could affect the exposure of the main components of Eu extract in rats due to the changed intestinal absorption.The research on the compatibility of Pc and Eu was helpful to guide the clinical administration of Eu and Pc simultaneously. 展开更多
关键词 double intubation drug interaction eucommia ulmoides Oliver LC/MS/MS pharmacokinetic properties Psoralea corylifolia L
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