[Objectives] The study aims to improve the ability of inspectors to recognize humoral morphology and provide data reference for the improvement of EQA work of humoral morphology by Guangxi Clinical Laboratory Center. ...[Objectives] The study aims to improve the ability of inspectors to recognize humoral morphology and provide data reference for the improvement of EQA work of humoral morphology by Guangxi Clinical Laboratory Center. [Methods] A retrospective analysis was made on the EQA of humoral morphology by Guangxi Clinical Laboratory Center from 2010 to 2022, and the number of participating laboratories, the rate of return, sample types and maps of EQA of humoral morphology such as urine sediment, vaginal secretions and so on(except feces), and the reasons for the high error rate of the results of the participating laboratories were analyzed. [Results] The number of laboratories participating in the assessment of humoral morphology increased from 98 in 2010 to 371 in 2022. Except for 2011(90.98%), the rate of return was more than 94%, and the highest rate of return was up to 100%. A total of 119 pictures of EQA of humoral morphology were studied, including 101 urine sediment smears, 13 vaginal secretions smears and 5 other smears. The types of specimens were relatively comprehensive. The distribution of the maps was basically reasonable. The urinary sediment was mainly composed of crystalline salts, followed by tubes, and cells and fungi ranked third. The coverage was relatively complete. The error rate of return results from high to low were bacteria(error rate was equal to 40%), tubes and crystalline salts(error rate was close to 29%), and cells and fungi(error rate was about 14%). The main cases with high error rate of return results are as follows: the difference between different tubes was not clear, and the diversity of crystal morphology was not understood enough;the phase division of cells in the same system was wrong. [Conclusions] The analysis and summary of the reasons for the high error rate of the data and results of Guangxi's EQA of humoral morphology is helpful for laboratory physicians to improve their awareness of morphological examination and identify clearly the error type and blurred concept, and lays a foundation for Guangxi Clinical Laboratory Center to carry out EQA of humoral morphology more specifically.展开更多
目的利用室间质量评价(EQA)分析上海地区临床实验室运动神经元存活(survival motor neuron,SMN)基因外显子缺失项目的检测能力。方法选用含有不同SMN外显子拷贝数的细胞株基因组DNA作为EQA样本,2023年间共两次,每次随机选取5份发放给上...目的利用室间质量评价(EQA)分析上海地区临床实验室运动神经元存活(survival motor neuron,SMN)基因外显子缺失项目的检测能力。方法选用含有不同SMN外显子拷贝数的细胞株基因组DNA作为EQA样本,2023年间共两次,每次随机选取5份发放给上海地区各参评实验室,要求其在规定时间内进行检测并上传结果,依据回报结果统计分析并评价其检测能力。结果两次EQA中SMN1判定结果回报全部正确的实验室分别占100%(38/38)和97.22%(35/36),SMN1第7,8号外显子拷贝数检测的总体符合率分别为96.92%(315/325)和98.18%(324/330),SMN2第7,8号外显子拷贝数检测的总体符合率分别为100%(65/65)和96.56%(43/45)。回报错误结果中包括1例结果判定错误和4例拷贝数检测错误。结论通过筛选出具有不同SMN外显子拷贝数的细胞株基因组DNA可有效模拟临床样本并应用于SMN外显子缺失EQA活动中。回报结果分析显示上海地区临床实验室SMN外显子缺失项目整体符合率较高,但个别实验室检测能力尚待提高。展开更多
This study aimed to evaluate the external and internal fluxes of nutrients of an urban eutrophic reservoir （lbirit6 reservoir, SE-Brazil）. External loads were estimated in the tributaries （Pintados and Ibirit6 cre...This study aimed to evaluate the external and internal fluxes of nutrients of an urban eutrophic reservoir （lbirit6 reservoir, SE-Brazil）. External loads were estimated in the tributaries （Pintados and Ibirit6 creeks） through nutrient concentrations and discharge measurements. Using Fick＇s law, internal loads were estimated in the reservoir from fluxes across sediment-water interface from nutrient concentration gradients between the pore water and the water. The Ibirit6 creek （urban sewage recipient） contributes with 72%-47% of ammonium （NH4^＋） and 100%-8% of SRP （soluble reactive phosphorus） of the total quantity entering the reservoir, whereas the Pintados creek （oil refinery effluent recipient） contributions are 20%-19% and 44%-100%, respectively. Despite the high external loads （130 and 2.2 ton-yr^-1 of NH4＋ and SRP, respectively）, internal loads （average flux of 120 and 2 mg·cm^-2yr^-1, respectively） correspond to 25% of the total external loads which may sustain a high productivity in the reservoir for a long time even if the external loads are controlled. The stocks of ammonium and SRP of the interstitial water （100 cm of sediment） would be released to the water in six years and five months, respectively, The release time would be extremely larger （〉 3,000 years） considering the stocks of total N and bioavailable P.展开更多
基金Supported by the Scientific Research Project of Guangxi Health Commission (Z2014221)。
文摘[Objectives] The study aims to improve the ability of inspectors to recognize humoral morphology and provide data reference for the improvement of EQA work of humoral morphology by Guangxi Clinical Laboratory Center. [Methods] A retrospective analysis was made on the EQA of humoral morphology by Guangxi Clinical Laboratory Center from 2010 to 2022, and the number of participating laboratories, the rate of return, sample types and maps of EQA of humoral morphology such as urine sediment, vaginal secretions and so on(except feces), and the reasons for the high error rate of the results of the participating laboratories were analyzed. [Results] The number of laboratories participating in the assessment of humoral morphology increased from 98 in 2010 to 371 in 2022. Except for 2011(90.98%), the rate of return was more than 94%, and the highest rate of return was up to 100%. A total of 119 pictures of EQA of humoral morphology were studied, including 101 urine sediment smears, 13 vaginal secretions smears and 5 other smears. The types of specimens were relatively comprehensive. The distribution of the maps was basically reasonable. The urinary sediment was mainly composed of crystalline salts, followed by tubes, and cells and fungi ranked third. The coverage was relatively complete. The error rate of return results from high to low were bacteria(error rate was equal to 40%), tubes and crystalline salts(error rate was close to 29%), and cells and fungi(error rate was about 14%). The main cases with high error rate of return results are as follows: the difference between different tubes was not clear, and the diversity of crystal morphology was not understood enough;the phase division of cells in the same system was wrong. [Conclusions] The analysis and summary of the reasons for the high error rate of the data and results of Guangxi's EQA of humoral morphology is helpful for laboratory physicians to improve their awareness of morphological examination and identify clearly the error type and blurred concept, and lays a foundation for Guangxi Clinical Laboratory Center to carry out EQA of humoral morphology more specifically.
文摘目的利用室间质量评价(EQA)分析上海地区临床实验室运动神经元存活(survival motor neuron,SMN)基因外显子缺失项目的检测能力。方法选用含有不同SMN外显子拷贝数的细胞株基因组DNA作为EQA样本,2023年间共两次,每次随机选取5份发放给上海地区各参评实验室,要求其在规定时间内进行检测并上传结果,依据回报结果统计分析并评价其检测能力。结果两次EQA中SMN1判定结果回报全部正确的实验室分别占100%(38/38)和97.22%(35/36),SMN1第7,8号外显子拷贝数检测的总体符合率分别为96.92%(315/325)和98.18%(324/330),SMN2第7,8号外显子拷贝数检测的总体符合率分别为100%(65/65)和96.56%(43/45)。回报错误结果中包括1例结果判定错误和4例拷贝数检测错误。结论通过筛选出具有不同SMN外显子拷贝数的细胞株基因组DNA可有效模拟临床样本并应用于SMN外显子缺失EQA活动中。回报结果分析显示上海地区临床实验室SMN外显子缺失项目整体符合率较高,但个别实验室检测能力尚待提高。
文摘This study aimed to evaluate the external and internal fluxes of nutrients of an urban eutrophic reservoir （lbirit6 reservoir, SE-Brazil）. External loads were estimated in the tributaries （Pintados and Ibirit6 creeks） through nutrient concentrations and discharge measurements. Using Fick＇s law, internal loads were estimated in the reservoir from fluxes across sediment-water interface from nutrient concentration gradients between the pore water and the water. The Ibirit6 creek （urban sewage recipient） contributes with 72%-47% of ammonium （NH4^＋） and 100%-8% of SRP （soluble reactive phosphorus） of the total quantity entering the reservoir, whereas the Pintados creek （oil refinery effluent recipient） contributions are 20%-19% and 44%-100%, respectively. Despite the high external loads （130 and 2.2 ton-yr^-1 of NH4＋ and SRP, respectively）, internal loads （average flux of 120 and 2 mg·cm^-2yr^-1, respectively） correspond to 25% of the total external loads which may sustain a high productivity in the reservoir for a long time even if the external loads are controlled. The stocks of ammonium and SRP of the interstitial water （100 cm of sediment） would be released to the water in six years and five months, respectively, The release time would be extremely larger （〉 3,000 years） considering the stocks of total N and bioavailable P.
