F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发...F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发现At5g22700蛋白的F-box结构域与ASK4蛋白相互作用。实时定量PCR分析该基因在不同组织器官中的表达,发现该基因在根和花中的表达量最高,说明At5g22700可能在根和花的发育中具有重要作用。以At5g22700基因的T-DNA插入突变体和过量表达转基因株系为材料,分析不同光照条件下幼苗的表型,发现蓝光下At5g22700过量表达转基因幼苗的主根比野生型长。这些研究结果表明,At5g22700在植物体内可能形成SCF复合体,并在植物幼苗主根伸长生长中起促进作用。展开更多
F-box protein is an expanding family member of eukaryotic protein characterized by an F-box motif which has specificity of substrate recognition in the ubiquitin-mediated proteolysis.These proteins have been proved to...F-box protein is an expanding family member of eukaryotic protein characterized by an F-box motif which has specificity of substrate recognition in the ubiquitin-mediated proteolysis.These proteins have been proved to be critical for many physiological processes,such as cell-cycle transition,signal transduction,gene transcription,male sterility,programmed cell death （PCD） and so on.This paper mainly introduces the biological functions of the known F-box proteins and the analysis of F-box gene phylogeny.展开更多
文摘F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发现At5g22700蛋白的F-box结构域与ASK4蛋白相互作用。实时定量PCR分析该基因在不同组织器官中的表达,发现该基因在根和花中的表达量最高,说明At5g22700可能在根和花的发育中具有重要作用。以At5g22700基因的T-DNA插入突变体和过量表达转基因株系为材料,分析不同光照条件下幼苗的表型,发现蓝光下At5g22700过量表达转基因幼苗的主根比野生型长。这些研究结果表明,At5g22700在植物体内可能形成SCF复合体,并在植物幼苗主根伸长生长中起促进作用。
基金Supported by the Cloning and Genetics Analysis of Cold-Induced Genes in PA64S, Natural Science Foundation of Hunan Province(09JJ3044)
文摘F-box protein is an expanding family member of eukaryotic protein characterized by an F-box motif which has specificity of substrate recognition in the ubiquitin-mediated proteolysis.These proteins have been proved to be critical for many physiological processes,such as cell-cycle transition,signal transduction,gene transcription,male sterility,programmed cell death （PCD） and so on.This paper mainly introduces the biological functions of the known F-box proteins and the analysis of F-box gene phylogeny.
