Electroacupuncture at Fengchi(GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine

Most migraine patients suffer from cutaneous allodynia; however, the underlying mechanisms are unclear. Calcitonin gene-related peptide（CGRP） plays an important role in the pathophysiology of migraine, and it is therefore, a potential therapeutic target for treating the pain. In the present study, a rat model of conscious migraine, induced by repeated electrical stimulation of the superior sagittal sinus, was established and treated with electroacupuncture at Fengchi（GB20）（depth of 2–3 mm, frequency of 2/15 Hz, intensity of 0.5–1.0 m A, 15 minutes/day, for 7 consecutive days）. Electroacupuncture at GB20 significantly alleviated the decrease in hind paw and facial withdrawal thresholds and significantly lessened the increase in the levels of CGRP in the trigeminal ganglion, trigeminal nucleus caudalis and ventroposterior medial thalamic nucleus in rats with migraine. No CGRP-positive cells were detected in the trigeminal nucleus caudalis or ventroposterior medial thalamic nucleus by immunofluorescence. Our findings suggest that electroacupuncture treatment ameliorates migraine pain and associated cutaneous allodynia by modulating the trigeminovascular system ascending pathway, at least in part by inhibiting CGRP expression in the trigeminal ganglion.

WEI JIA'S EXPERIENCE ON THE APPLICATION OF FENGCHI (GB 20)

The article introduces Prof.Weijia’s experience in using Fengchi(GB 20)totreat gastric ulcer,bronchial asthma,sciatica,and alopecia,etc.He considers that the point has seven general functions of dispelling pathogenic wind,purging pathogenic fire,relieving the depressedliver-Qi,resolving the phlegm,activating the blood,tranquilizing the mind,and checking spasm andconvulsion.It also introduces some common diseases treated with needling Fengchi and adjunctpoints,manipulations,and precautions,etc.It Is a comparatively comprehensive summary of Prof.Wei Jia’s individual experience in wonderfully using Fengchi.

TREATING 100 CASES OF HEADACHE BY MAINLY NEEDLING FENGCHI AND TAICHONG

This article introduces the writers’ experience in treating 100 cases of headache bymainly needling Fengchi (GB 20) and Taichong (LR 3). It is considered in TCM that headache ismostly caused by pathogenic wind, and there is a saying of "no wind, no headache". The combinationof the two points can expel both endogenous and exogenous wind. So we treat headache mainly withneedling Fengchi and Taichong. The result is good, especially for cluster headache and psychicheadache.’

CLINICAL APPLICATION OF FENGCHI(GB 20)

The article introduces the writers’ experience in using Fengchi(GB 20) to treat sciatica,gastric ulcer and bronchial asthma, etc. The writers consider that the point has seven general functions of dispelling pathogenic wind, purging pathogenic fire, relieving the depressed liver-qi, resolvingthe phlegm, activating the blood, tranquilizing the mind, and checking spasm and c0nvulsion. It alsointroduces the manipulations and precautions of needling Fengchi.

EFFECT OF ACUPUNCTURE OF FENGCHI (GB 20) ON BLOOD PRESSURE AND SERUM IL-6 and PLASMA ET LEVELS IN PATIENTS WITH HYPERTENSION

Objective：To observe the effect of acupuncture of Fengchi （风池 GB 20） on blood pressure, serum IL-6 and plasma endothelin （ET） contents in patients with hypertension. Methods： Thirty cases of hy-pertension patients were made up of treatment group and treated with acupuncture of Fengchi （GB 20） ; while 20 healthy subjects were made up of control group. Blood pressure was determined before and after the treatment. Fasting blood samples were taken from the cubital venous vessel for detecting serum interleukin （IL）-6 and plasma ET contents with radioimmunoassay before and after acupuncture treatment. Results： Before the treatment, in hypertension patients, serum IL-6 and plasma ET levels were significant higher thanthose in healthy subjects （P〈 0.01 ）; while after the treatment, blood pressure of treatment group declined significantly （ P 〈 0.01 ）, serum IL-6 and plasma ET levels decreased considerably （ P 〈 0.01 ）. Conclusion： Acupuncture of Fengchi （GB 20） can lower blood pressure, regulate cellular immunity and secretion functions of the vascular endothelial cells in hypertension patients.

ACUPUNCTURE TREATMENT OF OCCIPITAL NEURALGIA WITH DAZHUI AND FENGCHI ACUPOINTS

Objective: To explore therapeutic method for occipital neuralgia. Methods: A total of 90 occipital neuralgia patients were randomly divided into treatment group (n=60 cases) and control group (n=30). In treatment group, Dazhui (GV 14) was punctured and stimulated with reinforcing method, and Fengchi (GB 20) punctured and stimulated with reducing needling method. In control group, the tender point was punctured and stimulated with reducing needling method. The acupuncture needles were retained for 30 min and the treatment was conducted once daily, with 10 times being a therapeutic course. Results: After one session of treatment, the markedly effective rates of treatment and control groups were 98% and 57% respectively. Comparison between two groups showed a considerable difference in the therapeutic effect (P<0.05). Conclusion: Acupuncture of Dazhui (GV 14) and Fengchi (GB 20) are effective in the treatment of occipital neuralgia.

Effects of electroacupuncture at Fengchi(GB20)on motor function and GFAP/NeuN expression around the ischemic tissue of the motor cortex in MCAO rats

Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research and clinical applications.Methods:Forty male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups:a normal group,a normal with EA group,a model group,and a model with EA group,with 10 rats in each group.The normal group received no intervention.The normal with EA group received EA at bilateral Fengchi(GB20).The model group underwent middle cerebral artery occlusion(MCAO)using the suture.The model with EA group underwent MCAO and received EA at bilateral Fengchi(GB20).Cerebral blood flow was monitored using a laser Doppler cerebral blood flow meter.Neurologic damage was assessed using the neurologic deficit score,and motor ability was observed using the CatWalk gait system.The expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)protein,the neuron markers,was detected by Western blotting.The protein expression levels of GFAP and NeuN,as well as the number of positive cells in the motor cortex,were detected using immunofluorescence.Results:Compared to the normal group,the cerebral blood flow values in the model group and the model with EA group decreased by more than 50%during the modeling process(P<0.01)and returned to pre-modeling levels after reperfusion(P>0.05).The neurologic deficit score increased(P<0.05),the average motor velocity decreased(P<0.05),GFAP protein expression and the number of positive cells in the motor cortex increased(P<0.05),and the NeuN protein expression and the number of positive cells decreased(P<0.05)in the model group.Compared to the model group,the neurologic deficit score decreased(P<0.05),the average motor velocity accelerated(P<0.05),GFAP and NeuN protein expression and the number of positive cells in the motor cortex increased(P<0.01)in the model with EA group.Conclusion:EA at bilateral Fengchi(GB20)can reduce neuronal loss and increase GFAP and NeuN protein expression in the motor cortex of rats after ischemia-reperfusion,improve the motor function after ischemic stroke,and accelerate the recovery of balance and stability of the affected limbs.

Effect of acupuncture at Fengchi(GB 20)on the activity of myosin light chain kinase in the middle meningeal artery of migraine modeled rats

OBJECTIVE:To study the effect of acupuncture at Fengchi(GB 20) on the activation of myosin light chain kinase(MLCK) in the middle meningeal artery of migraine modeled rats.METHODS:Forty-four clean grade healthy female Sprague-Dawley(SD) rats were randomly divided into four groups:the control group,blank control group,Fengchi(GB 20) acupuncture group,and Fengchi(GB 20) prevention group.Neurogenic inflammation of these rats was induced by electrical stimulation.The γ-^(32)P infiltration method was then used to detect MLCK activation in the middle meningeal artery,and immunocytochemistry was applied to detect the structural protein expression of MLCK.RESULTS:The migraine model was successfully established in the rats.Compared with the control group,MLCK activation was significantly decreased in the blank control group(P < 0.01).CONCLUSION:The activation of MLCK in the middle meningeal artery was increased by acupuncture at Fengchi(GB 20),indicating its effectiveness in preventing and curing on acute migraine attacks.

电针风池穴对颈性高血压家兔血压和椎动脉、颈动脉血流动力学的影响

目的:探讨电针风池穴对颈性高血压模型兔血压、颈动脉和椎动脉血流动力学指标的影响。方法:建立颈性高血压模型,将造模成功的12只家兔按随机数字表法分为模型组和电针组,每组6只,另选取6只健康家兔作为正常组。对电针组家兔给予电针风池穴治疗,模型组和正常组家兔仅给予相同时间的固定。观察各组家兔椎动脉及颈动脉收缩期最大流速(peak systolic velocity, PSV)、舒张期最大流速(end-diastolic volume, EDV)、阻力指数(resistance index, RI)、股动脉收缩压(systolic blood pressure, SBP)变化。结果:与正常组比较,电针组家兔颈动脉收缩期血流速度[(61.30±9.62)cm/s]显著改善(P<0.05);与治疗前比较,电针组家兔股动脉收缩血压[(109.60±4.56)mm Hg]显著降低(P<0.05),模型组家兔股动脉收缩血压[(112.00±6.07)mm Hg]无明显变化。结论:电针风池穴可有效降低颈性高血压家兔血压,其降压作用可能与改善颈区血管血流动力学指标相关。

自拟清热润目汤联合朱氏风池三针法配合眼周取穴对老年睑板腺功能障碍致干眼的治疗效果

目的探究自拟清热润目汤联合朱氏风池三针法配合眼周取穴对老年睑板腺功能障碍致干眼的治疗效果。方法将2021年8月至2023年8月浙江省丽水市中心医院眼科收治的109例老年睑板腺功能障碍致干眼患者作为研究对象。根据患者接受的不同治疗方式进行分组,将接受常规西药治疗的患者纳入A组(53例),将接受西药治疗和自拟清热润目汤联合朱氏风池三针法配合眼周取穴治疗的患者纳入B组(56例),2组均治疗3个月。对比2组患者的治疗有效性、干眼程度[国际标准眼表疾病指数(OSDI)]、睑板腺功能(睑脂排出能力、眼睑分泌物性状)、角膜染色情况、非入侵泪膜破裂时间和并发症发生率(角膜溃疡、角膜穿孔、继发细菌感染)。结果B组的治疗有效率(95%)高于A组(81%)(P<0.05);B组患者的干眼程度[正常(63%)、轻度干眼(32%)、中度干眼(5%)、重度干眼(0)]低于A组[正常(41%)、轻度干眼(47%)、中度干眼(8%)、重度干眼(4%)](P<0.05);B组患者的睑脂排出能力正常率(73%)高于A组(51%),眼睑分泌物性状正常率(68%)高于A组(47%)(P<0.05);B组患者的角膜染色评分(4.6±0.7)分低于A组(6.5±0.8)分,非入侵泪膜破裂时间(11.0±1.3)s长于A组(10.3±1.1)s(P<0.05);B组并发症发生率(13%)与A组(8%)差异无统计学意义(P>0.05)。结论自拟清热润目汤联合朱氏风池三针法配合眼周取穴对老年睑板腺功能障碍致干眼的疗效显著,可有效缓解干眼症状、恢复眼睑功能、增强泪膜稳定性。

风池穴针刺不同角度、深度对老年脑卒中的疗效

目的观察不同角度和深度针刺风池穴对老年脑卒中患者的临床疗效。方法选取2022年7月-2023年7月长春骨伤医院收治的60例脑卒中合并吞咽障碍的老年患者作为研究对象。采用抽签法随机分为两组,各30例。对照组针刺风池穴按照常规针刺深度进行针刺,向鼻尖方向针刺0.8~1.2寸;观察组针刺风池穴时向喉结方向针刺1.5~2.0寸;两组同时均给予常规治疗。比较两组的临床疗效、中医症候、吞咽功能(洼田饮水试验及视频透视吞咽检查法)、神经功能[美国国立卫生研究院脑卒中量表(NIHSS)]、生活质量及不良反应。结果观察组总有效率高于对照组(P<0.05)。治疗后观察组口角歪斜程度、眼裂闭合程度和鼻唇沟深浅3项中医症候指标评分均低于对照组(P<0.05)。治疗后观察组洼田饮沙沙试验结果、NIHSS评分均低于对照组(P<0.05),且观察组的视频透视吞咽检查结果和生活质量评分均高于对照组(P<0.05)。两组不良反应发生情况比较,差异无统计学意义(P>0.05)。结论风池穴深刺和针向喉结方向对老年脑卒中患者的症状改善及预后有着明显作用,该方法安全可行。

风池穴“气至病所”针刺法对针刺治疗偏头痛疗效的影响

目的:观察风池穴“气至病所”针刺法对针刺治疗偏头痛临床疗效的影响。方法:将60例偏头痛患者按随机数字表法分为治疗组和对照组各30例。对照组取双侧太阳、丝竹空、率谷、风池、外关、侠溪穴,予以常规针刺治疗。治疗组取穴同对照组,风池穴在揣穴的基础上快速进针,缓慢调整针尖方向及针刺深度进行搜气,直至患者自觉头颞部有酸胀感,余穴操作同对照组。2组均连续治疗4周。比较2组首次治疗前后视觉模拟评分法(VAS)评分,以及治疗前、治疗4周后偏头痛特异性生活质量问卷(MSQ)和头痛症状积分,评价2组临床疗效。结果:首次治疗后,2组VAS评分均较治疗前降低(P<0.05),治疗组VAS评分低于对照组(P<0.05)。治疗4周后,2组MSQ评分均较治疗前降低(P<0.05),组间比较,差异无统计学意义(P>0.05)。治疗4周后,2组头痛发作次数、头痛程度、头痛时长、伴随症状积分及总分均较治疗前降低(P<0.05),上述评分组间比较,差异均无统计学意义(P>0.05)。治疗组总有效率90.00%,对照组总有效率86.67%,2组比较,差异无统计学意义(P>0.05)。结论:2种方法均能减轻偏头痛患者疼痛,改善其生活质量,但风池穴“气至病所”针刺法可提高即时镇痛效果。

电针风池穴对MCAO/R大鼠运动功能及运动皮层缺血周围灶Olig2蛋白的影响

目的探讨电针风池穴治疗脑缺血再灌注损伤的可能机制。方法将40只雄性SPF级SD大鼠随机分为空白组、空白电针组、模型组、模型电针组,每组10只。空白组不进行任何处理,空白电针组采用电针风池穴治疗,模型组进行线栓法大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfusion,MCAO/R)模型制备,模型电针组进行线栓法MCAO/R模型制备并采用电针风池穴治疗。4组在电针前后均进行体质量测量、Cat Walk步态检测,电针治疗结束后进行2,3,5-三苯基氯化四氮唑(2,3,5-Triphenyltetrazolium Chloride,TTC)染色观察脑梗死情况,蛋白免疫印迹(Western blot,WB)、免疫荧光检测少突胶质细胞转录因子2(oligodendrocyte transcription factor 2,Olig2)蛋白表达。结果与空白组比较,模型组大鼠脑梗死面积显著增大(P<0.01),体质量显著下降(P<0.01),四肢摆动速度明显减慢(P<0.05),左前脚掌宽度明显减小(P<0.05),WB及免疫荧光检测运动皮层区Olig2表达减少(P<0.05,P<0.01)。与模型组比较,模型电针组大鼠脑梗死面积明显减小(P<0.01),体质量增加(P<0.05),造模后2~3 d大鼠四肢摆动速度增加(P<0.05),造模后3~4 d大鼠左前脚掌宽度增大(P<0.05),WB及免疫荧光检测运动皮层缺血周围灶区域Olig2表达增加(P<0.01)。结论电针风池穴可上调再灌注后大鼠运动皮层区域缺血周围灶Olig2蛋白的表达,减小再灌注后大鼠脑组织梗死面积,促进运动皮层的修复,加速患肢平衡与稳定性的恢复。

基于主成分分析法的穴位按摩装置

基于主成分分析法设计了一套能精确寻找穴位的按摩装置,以替代中医按摩师进行风池穴按摩。首先,基于风池穴位于人脑后部中心、距离颈部最低点两侧的距离为手掌4指宽度1/2的特征,应用激光测距传感器、步进电机、直线位移传感器进行人后脑颈部最低点搜索并在后脑特定区域进行三维建模。然后,应用主成分分析法计算建立的三维模型应用风池穴周边点云的曲率及其法向量,得出按摩头的位置和摆角角度。最后,该装置将按摩头以计算的摆角角度移动到对应位置进行按摩。

针刺风池穴对脑血流的影响

应用经颅多普勒超声对97例患者进行针刺风池穴前后椎基底动脉血流速度观测,结果表明,治疗组高流速型与低流速型针刺前后血流速度比较均有显著变化(P<0.001),治疗组与对照组两样本针刺后比较有显著差别(P<0.001)。

风池穴的解剖结构和针刺深度

目的 :探讨风池穴的解剖结构和针刺深度。方法 :随机抽样取 57具较新鲜的成年人尸体 ,其中男尸 2 4具 ,女尸 3 3具 ,采用解剖断面法和解剖层次法进行研究。结果 :向下直刺的解剖结构依次是 :皮肤、浅筋膜、斜方肌与胸锁乳突肌之间、头夹肌、头半棘肌、头后大直肌。针刺的平均危险深度是 49.71mm。结论 :为了安全 ,建议风池穴针刺的深度控制在 3 4.

电针风池、供血穴治疗椎-基底动脉供血不足性眩晕的临床研究

目的探讨电针风池、供血穴治疗椎-基底动脉供血不足(VBI)眩晕的临床疗效。方法将55例符合入选标准的VBI眩晕患者随机分为电针组28例和西药组27例。电针组采用电针风池、供血穴治疗,西药组采用西比灵治疗,疗程均为2周。观察两组患者治疗前后眩晕症状积分、临床疗效以及双侧椎动脉(VA)、基底动脉(BA)平均血流速度(Vm)的变化。结果治疗后,电针组眩晕症状积分明显低于西药组(P<0.01),电针组LVA、RVA、BA的Vm值均高于西药组(P<0.05);电针组总有效率92.86%,西药组总有效率70.37%,两组比较差异有统计学意义(P<0.05)。结论电针风池、供血穴可缓解眩晕症状,改善VBI眩晕患者椎-基底动脉血流速度,临床疗效显著,值得进一步推广。

针刺太冲、曲池、风池穴治疗原发性高血压病的疗效对比研究

目的:比较针刺太冲、曲池、风池穴的降压疗效,对针刺治疗原发性高血压病进行穴位优选研究。方法:将90例原发性高血压病患者根据随机数字表分为太冲组、曲池组、风池组,分别针刺相应穴位,疗程2周,观察第一次针刺后即时血压、治疗前后平均血压及总有效率。结果:针刺后3组血压均较治疗前明显下降(P<0.05),其中太冲组、曲池组在即时降压疗效和长期降压疗效方面均优于风池组(P<0.05),而太冲组总有效率最高(P<0.05)。结论:针刺太冲、曲池、风池均具有降压作用,其中太冲穴降压疗效最好。

深刺风池穴对偏头痛患者脑血流动力学及血液流变学的影响

目的:探讨深刺风池穴对偏头痛患者脑血流动力学及血液流变学的影响。方法:本研究纳入偏头痛患者40例,分为深刺风池穴组(20例)和常规针刺风池穴组(20例),应用经颅多普勒检测治疗前后脑血流变化及血液流变仪检测治疗前后血流变学的改变。结果:深刺组有效率100%,常规针刺组有效率85%。两组治疗后大脑中动脉(MCA)、大脑前动脉(ACA)、大脑后动脉(PCA)及基底动脉(BA)血流量差异有显著性(P<0.05),而两组比较治疗前后血液流变学指标差异无显著性(P>0.05)。结论:深刺风池穴对偏头痛患者脑血管机能及脑血流变化可能有更好的调整作用。

电针四神聪和风池穴治疗血管性认知障碍临床研究

目的探讨电针四神聪和风池穴治疗血管性认知障碍的临床疗效及其对不同血管平均血流速度的影响。方法将60例血管性认知障碍患者随机分为电针四神聪和风池穴治疗组30例,传统针刺对照组30例。两组采用MMSE、ADL的Barthel指数评分及TCD评定受试者治疗前后的差异。结果治疗组总有效率为93.3%,对照组总有效率为80.0%,两组总有效率存在明显差异(P<0.05)。两组治疗前后MMSE、Barthel指数评分及各血管平均血流速度组内比较,差异均有统计学意义(P<0.05)。治疗后治疗组与对照组MMSE、Barthel指数评分及各血管平均血流速度有显著差异(P<0.05)。结论两组经治疗后,MMSE和Barthel指数评分及各血管平均血流速度均升高,但电针刺激四神聪和风池穴优于传统针刺治疗,可以提高疗效,是有效的治疗方法。