Antimicrobial,antibiofilm,angiogenesis,anti-inflammatory,and wound healing activities of zinc nanoparticles green synthesized using Ferula macrecolea extract

Objective:To assess the antimicrobial,antibiofilm,anti-inflammatory,angiogenic,and wound healing activities of zinc nanoparticles(ZNPs)green synthesized using Ferula macrecolea extract.Methods:The green synthesis was conducted using the precipitation method.Then,the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),and minimum biofilm inhibition concentration 50%(MBIC50)of ZNPs against Staphylococcus aureus(S.aureus)and Pseudomonas aeruginosa(P.aeruginosa)were evaluated.The effects of ZNPs on the gene expressions of Staphylococcus spp.[intracellular adhesion A(icaA)and D(icaD)]and P.aeruginosa(rhlI and rhlR)were investigated using quantitative real-time PCR.In addition,the effects of ZNPs on wound healing,angiogenesis,and anti-inflammatory markers were assessed.Results:The green-synthesized ZNPs demonstrated significant antimicrobial efficacy against S.aureus and P.aeruginosa.The biofilm formation in S.aureus and P.aeruginosa was also inhibited by ZNPs with MBIC50 values of 3.30μg/mL and 2.08μg/mL,respectively.Additionally,ZNPs downregulated the expression of biofilm-related genes icaA,icaD,rhlI,and rhlR in the tested bacteria.They also demonstrated promising in vitro wound healing effects by promoting fibroblast cell proliferation and wound closure in a dose-dependent manner.A significant increase in the expression of HLA-G5 and VEGF-A genes as well as a marked decrease in the expression of NF-κB,IL-1β,and TNF-αgenes were observed in cells treated with ZNPs compared to the control group(P<0.05).Conclusions:ZNPs display promising antibacterial effects against S.aureus and P.aeruginosa and wound-healing effects by inhibiting biofilm formation,inducing angiogenesis,and reducing inflammation.However,further studies must be conducted to specify the accurate mechanisms of action and toxicity of ZNPs.

Endophytic bacteria associated with endangered plant Ferula sinkiangensis K. M. Shen in an arid land： diversity and plant growth-promoting traits

Ferula spp. are traditional medicinal plants found in arid land. Large-scale excavation for extracting bioactive compounds from the plants in arid regions of Xinjiang over the last few years has, however, significandy decreased their distributions. Due to the urgent need for preservation of these plant resources, along with the need of searching for alternative source of the useful metabolites, it is important to screen the endophytic microbial resources associated with the plant Ferula sinkiangensis K. M. Shen. In the study, a total of 125 endophytic bacteria belonging to 3 phyla, 13 orders, 23 families, and 29 genera were isolated based on 16S rRNA gene sequencing data. Among the different isolates, three strains isolated from roots were potential novel species of the genera Porphyrobacter, Paracoccus and draycofatopsis. In this study, 79.4% and 57.1% of the total isolates were capable of producing indole-3-acetic acid （IAA） and siderophore, respectively. And, 40.6% of the strains inhibit the growth of fungal pathogen Afternaria alternata, 17.2% and 20.2% strains were positive for antagonism against Vertidllium dahlia 991 and V. dahlia 7, respectively. These results demonstrated that E sinkiangensis is a rich reservoir of endophytic bacterial resources with potential for production of biologically important functions such as plant growth-promoting factors.

Network pharmacology used to decode potential active ingredients in Ferula assafoetida and mechanisms for the application to Alzheimer’s disease

Background:Alzheimer’s disease(AD)is a neurodegenerative disease that primarily manifests as progressive memory loss and cognitive impairment.Traditional herbal medicines may be helpful in the discovery of new anti-AD drugs.Studies have shown that Ferula assafoetida has neuroprotective and memory-enhancing effects,which may be beneficial for the treatment of AD.However,the combination of active ingredients and their mechanisms remain unclear.Therefore,we aimed to identify potential active ingredients in F.assafoetida and their mechanisms of action against AD by using network pharmacology.Methods:In our study,an integrated network pharmacological approach,that included adsorption,distribution,metabolism and excretion screening,target identification,network construction,topological analysis,gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis,and molecular docking,was used to predict the pharmacological material basis and potential mechanisms through which these ingredients may treat and prevent AD.Results:The results indicated that 12 key active ingredients,obtained by topological analysis(including farnesiferol a,conferol,farnesiferol b,ferulic acid,etc.),may be the primary pharmacological components that may ameliorate AD.The 2 key significant pathways identified are the cholinergic synapse signaling pathway(critical targets include ACHE,CHRM1,CHRM2,MAPK1,PIK3CA,PIK3CB,PIK3CD,and PIK3CG)and the AD signaling pathway(critical targets include APP,BACE1,GSK3B,MAPK1,NCSTN,NOS1,PSEN1).These critical targets are closely related to the regulation of three typical pathological features of AD[central nervous system(CNS)cholinergic hypofunction,amyloid-β(Aβ)plaques,and hyperphosphorylated tau proteins].Finally,14 critical targets in the 2 key significant pathways were validated by molecular docking analysis.Conclusion:F.assafoetida may be effective for alleviating AD symptoms,through multi-component,multi-target,and multi-pathway synergistic effects,associated with the multiple pathogenesis hypotheses of AD.Our study may provide certain clues for the further development and utilization of this natural herbal medicine.

Anticancer potential of Ferula assa-foetida and its constituents,a powerful plant for cancer therapy

Cancer is one of the main challenges of the health system around the world.This disease is increasing in developing countries and imposes heavy costs on patients and governments.On the other hand,despite various drugs,the death rate among cancer patients is still high and the current treatments have many harmful effects.In the traditional medicine of different countries,there are many medicinal plants that can be effective in the treatment of cancer.Ferula plants are traditionally used as spices and food or for medicinal purposes.Ferula assa-foetida is one of the famous plants of this genus,which has been used for the treatment of various diseases since ancient times.Among the main compounds of this plant,we can mention monoterpenes,sulfide compounds and polyphenols,which can show different therapeutic effects.This article has been compiled with the aim of collecting evidence and articles related to the anti-cancer effects of extracts,derived compounds,essential oils and nanoparticles containing Ferula assa-foetida.This review article was prepared by searching the terms Ferula assa-foetida and cancer,and relevant information was collected through searching electronic databases such as ISI Web of Knowledge,PubMed,and Google Scholar.Fortunately,the results of this review showed that relatively comprehensive studies have been conducted in this field and shown that Ferula assa-foetida can be very promising in the treatment of cancer.

Protective effect of active ingredient of Ferula sinkiangensis on global cerebral ischemia-reperfusion mice

OBJECTIVE Learning and memory impairment is one of the common sequelae of stroke patients,which is called"post-stroke dementia"and seriously affects the quality of life of the patients.For post-stroke dementia,there is still no effective clinical treatment.In the present study,we aim to investigate the effect of the active ingredient of Ferula sinkiangensis,AW09,on global cerebral ischemia-reperfusion mice.METHODS The bilateral common carotid artery occlusion(BCCAO)reperfusion model was used to investigate the protective effect of AW09 on cognitive dysfunction in mice with global cerebral ischemia reperfusion.Y-maze and Morris water maze were used to test the learning and memory ability of mice.RESULTS Y-maze test showed that AW09 treatment significantly increased the spontaneous alternation rate of BCCAO model animals and had no significant effect on the total number of arm entries.The results of Morris water maze showed that AW09 significantly reduced the escape latency of BCCAO mice during the training period.During the probe test phase,AW09 significantly increased the swimming time in target quadrant,distance in target quadrant and number of platform crossings and decreased the swimming time in the quadrant opposite the target quadrant of BCCAO mice.CONCLUSION AW09,the active ingredient of Ferula sinkiangensis,can improve working memory impairment and spatial memory impairment in animals with global cerebral ischemia-reperfusion,suggesting that AW09 has poten⁃tial therapeutic value for cognitive dysfunction caused by global cerebral ischemia.

Mutation-Screening of Pleurotus Ferulae with High Temperature Tolerance by Nitrogen Ion Implantation

In order to obtain Pleurotus ferulae with high temperature tolerance, conidiophores of wild type strain ACK were implanted with nitrogen ions in energy of 5 -15 keV and dose of 1.5×10^15 - 1.5 × 10^16 cm^-2, and a mutant CGMCC1763 was isolated subsequently through thermotolerant screening method. It was found that during riper period the surface layer mycelium of the mutant in mushroom bag wasn＇t aging neither grew tegument even above 30℃. The mycelium endurable temperature of the mutant was increased by 5℃ compared to that of the wild type strain. The fruiting bodies growth temperature of the mutant was 18 -22℃ in daytime and 8 -14℃ at night. The highest growth temperature of fruiting bodies of the mutant was increased about 7℃ w.r.t, that of original strain. Through three generations investigations, it was found that the mutant CGMCC1763 was stable with high temperature tolerance.

Determination of Volatile Oil and Ferulic Acid in Different Parts of Wild Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M.Shen Cultivars

[Objectives] To increase the reserves distribution and planting area of Ferula resources,protect wild Ferula resources,make comparative analysis on wild and cultivated varieties of Ferula medicinal materials,and provide references for quality evaluation of Ferula herbs collected from cultivation environment. [Methods] Volatile oil determination and high performance liquid chromatography were used to measure volatile oil and ferulic acid in different parts of wild and cultivated varieties of Ferula. [Results] In the volatile oil measured from artificially pressed Ferula lipid,wild varieties in different places conformed to the pharmacopoeia standard( volatile oil content ≥10%); in cultivated varieties,F. fukanensis K. M. Shen and F. sinkiangensis K. M. shen reached the pharmacopoeia standard. According to measurement results of ferulic acid,the ferulic acid content of roots and leaves of Altay,Fukang,and Yining cultivars were higher than that of wild varieties. [Conclusions]The ferulic medicinal herbs collected from artificial cultivation environment have considerable prospect,and many parts of Ferula herbs can be used for raw materials for preparation of extracts.

Comparative Analysis of the Fruit Pericarp Structure of the Desert and Mountain Species of <i>Ferula</i>L. (Apiaceae Lindl.)

It has described structure of the pericarp of 6 Ferula species fruits in natural habitat conditions: Nuratau range and Kyzylkum Desert. Differences in the location and structure of the secretory conceptacles have been discovered due to their biomes and habitat conditions. Secretory tubules of mature fruit pericarp (Scorodosma subgenus) F. foetida obliterated. In the pericarp (Macrorrhiza section) of F. varia fruit, there are two types of secretory structures: dorsal and commissural. In the fruit pericarp of (Xeronarthex section) F. kyzylkumica, F. helenae, F. dshizakensis and F. angreni species, there are three types: dorsal, commissural and costal, which increase their drought resistance and reflect a higher evolutionary level of taxa [1].

The Biology of Flowering of Some Pamir-Alai Species of the Genus <i>ferula</i>L.

The sources about the seasonal and blossoming biology of some Ferula L. (F angrenii, F. ovina, F. dshizakensis, F. helenae) types which can be found in Western Pamir-Alay have been given in the article.

Ecological Analysis of Species of the Genus Ferula L., Distributed in Navoi Region (Uzbekistan)

Ferula L. is a perennial herbaceous plant belonging to the family Apiaceae Lindl. (Umbelliferae). Our research was carried out on the territory of Navoi region. The article presents an ecological analysis of the species of the Ferula L. in Navoi region. The species of genus Ferula mostly grows in mountainous regions and some are distributed in arid climates. According to that 15 species of the genus were found in the Navoi region, most of these species are considered to be plants that contain tar as well as have a strong medicinal property. In various parts of the world, different species of Ferula have been used in traditional medicine. In the course of studies, it was found that the species has medicinal, essential-oil, honey-juicy, nutritious, and fodder properties. According to ecological analysis, the genus Ferula grows Rocky, gravelly, fine-grained slopes and ridges of mountains, scree, rocks, outcrops of variegated rocks, rocky slopes, sandy, clay and gravelly deserts, foothill plains, fine-grained slopes. Considered rare, Ferula kyzylkumica Korovin is listed in the Red Book of the Republic of Uzbekistan, while Ferula nuratavica Pimenov is an endemic plant of the Aktau ridge.

阿魏侧耳(Pleuratus ferulae)产漆酶条件的优化及其酶学性质研究

为获得酶活较高的酶液用于农药污染的修复,探讨阿魏侧耳(Pleuratus ferulae)产漆酶的条件。对碳源、氮源、pH诱导剂和金属离子这5个培养条件进行优化,并对酶学性质进行研究。结果表明:阿魏侧耳产漆酶的最佳培养时间为9天,最佳碳源为可溶性淀粉,最佳氮源为蛋白胨,培养基最适的初始pH 6.0,0.1 mmol/L愈创木酚和1 mmol/L Mn^(2+)对阿魏侧耳产漆酶有明显的促进作用,使酶活分别提高了32.2%和30.8%。对阿魏侧耳产的漆酶进行酶学性质研究,其最适反应温度为45℃,在40、45℃下酶活相对稳定,48 h后能保持较高酶活。最适反应pH 6.0,在pH 4.0~7.0时,漆酶均有较高活性,在pH 5.0~7.0时有较高稳定性,48 h后相对酶活保持在70%以上。阿魏侧耳在发酵条件优化后具有较高的产漆酶能力,所产漆酶的最适温度相对较高,最适pH为中性偏酸性。

Structure-guided isolation of anti-neuroinflammatory sesquiterpene coumarins from Ferula sinkiangensis

The resin of Ferula sinkiangensis has been traditionally utilized for treating gastrointestinal disorders,inflammation,tumors,various cancers,and alopecia areata.The primary bioactive constituents,sesquiterpene coumarins,have demonstrated notable therapeutic potential against neuroinflammation.In this study,a structure-guided fractionation method was used to isolate nine novel sesquiterpene coumarins from the resin of F.sinkiangensis.These compounds were characterized and structurally elucidated using comprehensive physicochemical and spectroscopic techniques,including calculated electronic circular dichroism(ECD).Anti-neuroinflammatory assays revealed that compounds 2,3,and 6 significantly inhibited nitric oxide(NO)production in lipopolysaccharide(LPS)-stimulated BV2 microglial cells,with IC_(50)values ranging from 1.63 to 12.25μmol·L^(−1).

温度对托里阿魏(Ferula krylovii)和骆驼蓬(Peganum harmala)的呼吸及光合作用的影响

比较了荒漠短命药用植物托里阿魏(Ferula krylovii)及其伴生的非短命植物骆驼蓬(Peganum harmala)的呼吸和光合作用的温度响应。结果表明:以每克叶片鲜重耗氧量为基础的叶片呼吸速率来看,托里阿魏在30℃时突然出现呼吸速率的大幅度跃迁,在温度达到35℃时出现了呼吸系统快速失活现象,在45℃时呼吸速率达到20℃的3.1倍。相比之下,骆驼蓬叶片在20~45℃的呼吸速率没有明显的变化,也没有出现呼吸系统的失活现象。这说明,与处于同一生境的非短命植物骆驼蓬相比,托里阿魏的呼吸系统对温度变化有高度的敏感性,对高温的耐受性较差。托里阿魏叶片的光合作用在20~35℃连续升高并明显高于骆驼蓬,在35℃时达到最高点,在40~45℃时光合系统在光照条件下数分钟内快速失活。相比之下,骆驼蓬叶片的光合作用在40℃时达到最大值,在45℃时光合速率略有下降,但在20~45℃始终保持准线性变化,没有出现失活,表明夏季不休眠的骆驼蓬叶片的光合系统对高温有较高的耐受性。由于托里阿魏无论是呼吸和光合系统对温度的耐受性都明显低于非短命植物骆驼蓬,这种代谢上对温度的敏感性差异,可能是导致托里阿魏进入初夏休眠的主要因素。

托里阿魏挥发油的GC-MS指纹图谱及抗宫颈癌作用研究

目的建立托里阿魏挥发油的气相色谱-质谱(GC-MS)指纹图谱,并考察托里阿魏挥发油的抗宫颈癌作用。方法采用有机溶剂提取法提取托里阿魏根的挥发油,并通过GC-MS鉴定所含成分。通过MTT比色法考察不同浓度托里阿魏挥发油对人宫颈癌Hela细胞增殖的抑制作用。采用Transwell法检测托里阿魏挥发油对人宫颈癌Hela细胞迁移、侵袭能力的影响。应用流式细胞术检测托里阿魏挥发油作用下的细胞凋亡率。结果托里阿魏挥发油中鉴别出58种化学成分,含量最多的是孕酮。托里阿魏挥发油对人宫颈癌Hela细胞的增殖有一定的抑制作用,IC50为55.21μg/ml。托里阿魏高、低剂量组与对照组相比,迁移和侵袭细胞数量显著减少(P<0.01)。结论托里阿魏挥发油中含量最高的是酮类化合物。托里阿魏提取物有一定的抗肿瘤作用。

栽培和野生大果阿魏营养成分的分析和比较

测定栽培和野生大果阿魏的营养成分、重金属含量,栽培大果阿魏的挥发油成分和农药残留量,通过氨基酸评分(AAS)、化学评分(CSAA)、氨基酸比值系数评分(SRCAA)和必需氨基酸指数(EAAI)评价大果阿魏蛋白质的营养价值。结果显示:2种大果阿魏中营养成分丰富,总膳食纤维含量(栽培7.93%,野生8.62%)远高于其他富含纤维的蔬菜;两者氨基酸的营养价值相当,重金属含量均低于国家限量标准;栽培大果阿魏中维生素总含量明显高于野生大果阿魏,其挥发油主要为萜类成分;农药残留量远低于国家食品安全标准。

基于蛋白质组学探讨准噶尔阿魏抗结肠癌的作用机制

目的通过动物实验分析准噶尔阿魏乙醇提取物(简称醇提物)的体内抗结肠癌活性,基于蛋白质组学探讨准噶尔阿魏醇提物治疗结肠癌可能涉及的药理作用机制。方法建立结肠癌CT26荷瘤小鼠模型,随机分为正常对照组、模型组、顺铂组及准噶尔阿魏醇提物低、中、高剂量组,连续给药12 d,取材并计算各组抑瘤率及脏器指数。取模型组和抑瘤率>40%的准噶尔阿魏醇提物剂量组小鼠肿瘤组织标本,利用串联质谱标记(TMT)技术筛选准噶尔阿魏醇提物组与模型组间的差异蛋白,分析涉及的生物功能、关键作用靶标及信号通路。结果准噶尔阿魏醇提物高、中、低剂量组的抑瘤率分别为(63.82±4.01)%、(38.17±3.78)%、(36.25±3.43)%,准噶尔阿魏醇提物高剂量组的抑瘤率明显高于准噶尔阿魏醇提物中、低剂量组(P<0.05)。与正常对照组相比,顺铂组及准噶尔阿魏醇提物高、中、低剂量组小鼠的脾重、肾重及胸腺重均明显减小(P<0.05);准噶尔阿魏醇提物高、中、低剂量组间小鼠的脾重、肾重及胸腺重比较,差异均无统计学意义(P>0.05)。与正常对照组相比,准噶尔阿魏醇提物高剂量组小鼠的脾指数、胸腺指数明显减小(P<0.05),肾指数差异无统计学意义(P>0.05);与正常对照组相比,准噶尔阿魏醇提物中剂量组小鼠的脾指数、肾指数、胸腺指数均明显减小(P<0.05);与正常对照组相比,准噶尔阿魏醇提物低剂量组小鼠的肾指数、胸腺指数明显减小(P<0.05),脾指数差异无统计学意义(P>0.05);准噶尔阿魏醇提物高剂量组小鼠的肾指数明显高于准噶尔阿魏醇提物中、低剂量组(P<0.05)。蛋白质组学筛选出642个差异蛋白,其中FN1、FLNC、HSPH1、HSP90AA1等蛋白排名在前10;COG分析结果显示差异蛋白共参与了582个生物功能,主要涉及翻译后修饰及蛋白转运(15.8%)、信号转导机制(15.1%)、转录(9.1%)等;KEGG富集分析显示差异蛋白参与的信号通路主要涉及PI3K-Akt信号通路、细胞外基质-受体相互作用、癌症中的蛋白聚糖、补体和凝血级联反应、TNF信号通路、AMPK信号通路、甘氨酸丝氨酸和苏氨酸代谢等。结论动物实验显示准噶尔阿魏醇提物的抗结肠癌活性好,蛋白质组学分析揭示了准噶尔阿魏治疗结肠癌可能的关键靶标及分子机制,FN1、FLNC、HSPH1、HSP90AA1等可能是其中的关键蛋白。

圆锥茎阿魏的成分分离鉴定及葡萄糖摄取活性研究

目的探究圆锥茎阿魏(Ferula conocaula Korovin)的成分及药理活性。方法采用多种技术对圆锥茎阿魏石油醚部位与乙酸乙酯部位进行分离纯化,用现代波谱方法对化合物进行结构鉴定,并对分离得到的化合物进行葡萄糖摄取试验。结果从圆锥茎阿魏中分离得到20个化合物,分别为正十六烷酸(1)、豆甾醇(2)、β-谷甾醇(3)、莨菪亭(4)、香草醛(5)、pinoresinol(6)、硝基丙酸(7)、4-羟基苯乙基乙酸酯(8)、焦谷氨酸乙酯(9)、香草酸(10)、阿魏酸(11)、咖啡酸乙酯(12)、malyngicacid(13)、木犀草素(14)、(2E,4E)-sinkiangenoxide(15)、绿原酸甲酯(16)、(2Z,4E)-sinkiangenoxide(17)、二氢菜豆酸(18)、异香草酸(19)、酪醇(20),20个化合物均为首次从该植物中分离得到。葡萄糖摄取试验结果显示化合物6、18具有较强的促葡萄糖摄取活性。结论通过多种技术手段从圆锥茎阿魏中提取分离了20个化合物,均为首次从该种植物中分离得到,且pinoresinol、二氢菜豆酸具有显著的降血糖效果。

新疆阿魏含药血清对HeLa细胞凋亡的影响

目的研究新疆阿魏含药血清对HeLa细胞凋亡及相关蛋白Survivin和Caspase-3的影响。方法大鼠灌胃新疆阿魏获得含药血清,用含药血清处理HeLa细胞,采用CCK-8法测定细胞增殖抑制率,采用Hoechst33258染色联合流式细胞仪检测细胞凋亡,运用Western Blot法检测Survivin和Caspase-3蛋白表达。结果各浓度含药血清对细胞增殖抑制率分别为(11.4%±2.3%)、(14.9%±3.1%)、(25.3%±3.2%),细胞凋亡率分别为(29.4%±9.9%)、(35.8%±5.3%)、(43.1%±4.7%),各浓度含药血清组细胞中Survivin蛋白降低、Caspase-3蛋白升高(P<0.05)。与空白对照组比较,上述结果差异有统计学意义(P<0.01)。结论新疆阿魏含药血清促进HeLa细胞凋亡,可能与抑制Survivin蛋白表达和提高Caspase-3蛋白表达有关。

基于网络药理学研究阿魏、九香虫治疗胃癌的作用机制

目的 通过网络药理学方法探讨阿魏、九香虫治疗胃癌的作用机制。方法 利用中药综合数据库(Traditional Chinese Medicines Integrated Database,TCMID)与文献检索获取阿魏、九香虫的活性成分,将化学活性成分导入PubChem统一格式。利用SwissTargetPrediction数据库预测活性成分靶点,利用比较毒物基因组学数据库(Compargtive Toxicogenomics Database,CTD)获取胃癌靶点,并与药物所对应的靶点比较,筛选出共同部分,然后采用Cytoscape3.8.0软件搭建“药物-成分-靶点-疾病”网络。通过String数据库获取共有靶点之间的相互作用关系,并筛选出关键靶点。将共有靶点导入注释、可视化和集成数据库(the Database for Annotation, Visualization and Integrated Discovery,DAVID)进行基因本体(gene ontology,GO)分析、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果 从阿魏中获得抗胃癌相关活性成分15个,九香虫21个,从阿魏、九香虫中发现共同作用于胃癌的靶点112个。获得丝裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)、蛋白激酶B1(protein kinase B1,AKT1)、热休克蛋白90α家族A类成员1(heat shock protein 90α family class A member 1,HSP90AA1)、信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)、SRC基因等5个与胃癌相关的关键靶点,与胃癌密切联系的通路有磷脂酰肌醇3激酶-蛋白激酶B(phosphatidylinositol 3-kinase-protein kinase B,PI3K-Akt)信号通路、MAPK信号通路、细胞周期通路等。结论 阿魏、九香虫能够影响小血管生成,抑制细胞增殖、分化,促进细胞凋亡,可能通过抑制细胞周期G1期、G2/M期来发挥抗胃癌作用。

新疆阿魏乙酸乙酯部位体内外对CT-26.WT细胞的影响

目的研究新疆阿魏(Ferula sinkiangensis K.M.Shen)乙酸乙酯部位体内外对鼠源性结肠癌细胞(CT-26.WT)的影响。方法采用Cell Counting Kit-8(CCK-8试剂盒)法检测新疆阿魏乙酸乙酯部位体外对CT-26.WT细胞的抗肿瘤活性,计算细胞增殖抑制率、半数抑制浓度(IC50);采用流式细胞术检测新疆阿魏对CT-26.WT细胞的总凋亡率;建立小鼠CT-26.WT实体瘤模型,随机分为正常对照组,肿瘤模型组,阿魏低、中、高剂量组,顺铂组,给药10 d后观察小鼠脏器指数、体质量、肿瘤病理切片,计算肿瘤抑制率。结果新疆阿魏乙酸乙酯部位对体外CT-26.WT肿瘤细胞的IC50为28.23μg/m L,而顺铂为4.81μg/m L;低、中、高剂量新疆阿魏乙酸乙酯部位对CT-26.WT细胞具有促凋亡作用;对小鼠肿瘤抑制率分别为(38.26±7.82)%、(48.60±9.09)%和(66.54±12.02)%,而顺铂的抑制率为(95.18±3.02)%,新疆阿魏乙酸乙酯部位中、高剂量组脾指数均明显升高,与正常对照组相比差异有统计学意义,与模型组相比差异有统计学意义。胸腺指数和体质量与正常对照组比较无明显变化。结论新疆阿魏乙酸乙酯部位在体内外对CT-26.WT肿瘤生长具有一定的抑制作用。