2019年7月24日,世界知识产权组织(WIPO)发布了2019年全球创新指数(Global Innovation Index 2019,GII),基于80项指标对129个经济体进行排名,评出了各个地区的创新领导者。北美:美国(3),在信贷和投资市场质量方面保持全球领先,并且拥有...2019年7月24日,世界知识产权组织(WIPO)发布了2019年全球创新指数(Global Innovation Index 2019,GII),基于80项指标对129个经济体进行排名,评出了各个地区的创新领导者。北美:美国(3),在信贷和投资市场质量方面保持全球领先,并且拥有研发密集型的全球公司与顶尖的科学出版物和高校;在创新质量方面居全球之冠;是世界技术集群百强中入围最多的国家(26个)。展开更多
Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduc...Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.展开更多
文摘2019年7月24日,世界知识产权组织(WIPO)发布了2019年全球创新指数(Global Innovation Index 2019,GII),基于80项指标对129个经济体进行排名,评出了各个地区的创新领导者。北美:美国(3),在信贷和投资市场质量方面保持全球领先,并且拥有研发密集型的全球公司与顶尖的科学出版物和高校;在创新质量方面居全球之冠;是世界技术集群百强中入围最多的国家(26个)。
基金funded by the Science and Technology Development Fund,Macao SAR(Nos.0065/2020/A2,SKLQRCM(MUST)-2020-2022)Shenzhen-Hong Kong-Macao Science and Technology Project(Grade c)(No.SGDX20210823104201010).
文摘Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.
