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Expression Pattern of the Foreign Gene Regulated by the Tomato rbcS3A Promoter in Transgenic Tomato
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作者 徐晓霞 金烨 杨万年 《Agricultural Science & Technology》 CAS 2013年第4期559-563,共5页
[Objective] This study aimed to reveal the expression pattern of foreign genes regulated by tomato rbcS3A promoter in transgenic tomato. [Method] Rubisco small subunit promoter rbcS3A was cloned by PCR, fused to the u... [Objective] This study aimed to reveal the expression pattern of foreign genes regulated by tomato rbcS3A promoter in transgenic tomato. [Method] Rubisco small subunit promoter rbcS3A was cloned by PCR, fused to the upstream of Gus coding region in a binary vector, and transformed into tomato plants mediated by Agrobacterium. Histochemical staining on PCR positive plants was performed to ana- lyze the expression pattern of the foreign gene regulated by the tomato rbcS3A pro- moter in transgenic tomato. [Result] A total of 15 positive plants were obtained, ac- counting for 33.3%. Histochemical staining showed that the expression level of Gus fusion gene was highest in mature leaf, lower in reproductive organs such as fruit, and not detected in seed. [Conclusion] More positive seedlings were obtained using the modified tissue culture method. Under the control of tomato rbcS3A promoter, exogenous gene highly expressed in transgenic plant leaves, but did not express in seeds and tomato pulp. 展开更多
关键词 Tomato rbcS3A promoter TOMATO Genetic transformation gus staining
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Expression analysis of two reverse duplicated small heat shock protein genes in rice(Oryza sativa L.) 被引量:1
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作者 GUO Hong-xia ZENG Wen-zhi +8 位作者 WANG Chuang-yun FENG Jing-lei TANG Hui-wu BAI Mei LIU Yao-guang ZHAO Li WANG Lu-jun FAN Tao GUO Jing-xin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第8期1685-1692,共8页
supported by grants from the National Natural Science Foundation of China (30671178);the Shanxi Province Science Foundation for Youths, China (2014021029-2)
关键词 small heat shock proteins(sHSPs) expression characteristics semi-quantitative RT-PCR gus staining rice
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Efficient Regeneration and Agrobacterium-mediated Transformation of Brassica napus Cultivar Qingza No.5
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作者 Yajing XIE Aihua WU Xianjin LIU 《Agricultural Biotechnology》 CAS 2016年第3期48-53,56,共7页
Using the hypocotyl and cotyledon explants of Brassica napus L. cuhivar Qingza No. 5 as receptors, hormone combinations in bud differentiation medi- um, bud growth medium and rooting medium were optimized to establish... Using the hypocotyl and cotyledon explants of Brassica napus L. cuhivar Qingza No. 5 as receptors, hormone combinations in bud differentiation medi- um, bud growth medium and rooting medium were optimized to establish an efficient plantlet regeneration system of B. napus cuhivar Qingza No. 5. The results showed that the highest differentiation efficiency of hypocotyls of B. napus cuhivar Qingza No. 5 reached about 90%, which was three times that of cotyledons. The appropriate differentiation medium was MSB + 5 mg/L thidiazuron (TDZ) +7.5 mg/L AgNO3 + 0.1 mg/L NAA + 2 mg/L proline (L-pro) + 250 mg/L casein acid hydrolysate (CH) + 3% sucrose; the appropriate growth medium was 1/2 MSB + 1 mg/L IBA + 2 mg/L L-pro + 250 mg/L CH + 1.5% sucrose; the ap- propriate rooting medium was 1/2 MSB + 0.2 mg/L IAA + 1.5% sucrose. On this basis, a binary expression vector harboring insect resistance gene B12 was constructed and introduced into B. napus hypocotyls by Agrobacterium-mediated transformation. Positive plants were screened using hygromycin and carbenicillin. Transgenic plants were verified by PCR and GUS histochemical staining. The results showed that insect resistance gene B12 was successfully integrated into the nu- clear genome of B. napus plants and could be expressed normally. Leaves of transgenic plants with high expression levels were collected for indoor inoculation test with Plutella xylotella larvae to evaluate insect resistance of transgenic plants. 展开更多
关键词 words Brassica napus L. Efficient regeneration from hypocotyls Agrobacterium-mediated transformation Insect resistance genes gus staining Transgenicplants
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Functional Characterization of the Promoter and Second Intron of CUM1 During Flower Development in Cucumber(Cucumis sativus L.) 被引量:9
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作者 GU Ran LIU Xiaofeng +4 位作者 ZHAO Wensheng YAN Shuangshuang SUN Linhan WU Binning ZHANG Xiaolan 《Horticultural Plant Journal》 SCIE 2018年第3期103-110,共8页
The characterization of flower specific promoter is critical during flower development by cucumber transformation technology.AGAMOUS(AG)is an organ identity gene that is required for carpel and stamen development in A... The characterization of flower specific promoter is critical during flower development by cucumber transformation technology.AGAMOUS(AG)is an organ identity gene that is required for carpel and stamen development in Arabidopsis.The promoter and second intron of AG contain multiple regulatory elements that confer proper spatial and temporal expression.Cucumber is an important vegetable with unisexual flowers.Cucumber MADS-box 1(CUM1)is the AG homolog in cucumber,belonging to the eu AG lineage along with AG.In situ hybridization showed that CUM1 was specifically expressed in the stamens and carpels of cucumber.GUS staining indicated that the second intron of CUM1 confers stamen-specific expression,while the promoter of CUM1 drives both stamen-and carpel-specific expression during the early stages of flower development,but is restricted to carpel-and connectivum-specific expression during the late stages of flower development.Furthermore,a yeast one-hybrid assay demonstrated that two auxin response factors(Cs ARF13 and Cs ARF17)had bound directly to the second intron of CUM1.Our data suggest that different regulatory circuits operate in AG homologs in plant species with distinct sex types. 展开更多
关键词 CUCUMBER CUM1 PROMOTER INTRON in situ hybridization gus staining yeast one-hybrid
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