The authors utilized the polymerase chain reaction to rapidly and accurately type a variable number of tandemly repeated loci (VNTR loci) of the 3’ flanking region of apolipoprotein B (apo B) gene, and studied the re...The authors utilized the polymerase chain reaction to rapidly and accurately type a variable number of tandemly repeated loci (VNTR loci) of the 3’ flanking region of apolipoprotein B (apo B) gene, and studied the relationship between this loci polymorphisms and coronary heart disease (CAD); comparisons were made with relative diseases and展开更多
The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants w...The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.展开更多
Brachytic stem is a major trait in plant type .of soybean and its yield potential may be higher under high population when compared with normal stem. In the present investigation, 152 recombinant inbred line （RIL） f...Brachytic stem is a major trait in plant type .of soybean and its yield potential may be higher under high population when compared with normal stem. In the present investigation, 152 recombinant inbred line （RIL） families derived from the cross of Bogao （normal stem） and Nannong 94-156 （brachytic stem） were used to map genes and QTLs of three plant type traits and to identify the effects of brachytic stem on agronomic traits such as yield. The primary results indicated that brachytic stem （sb） and determinate growth habit （drl） were mapped on linkage groups B2 and L, three major QTLs related to plant height were detected and mapped on linkage group L near drl, another minor QTL was mapped near sb on linkage group B2-1. Lines with brachytic stem had shorter plant height, lower biomass, yield, harvest index and pods per plant, and essentially no differences in days to maturity and 100-seed weight when compared with normal stem lines. It was obvious that the effect of brachytic stem on yield was due to the decreased height, biomass and harvest index.展开更多
The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative...The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.展开更多
旨在基于基因多态性分析Wnt家族成员3a(wnt family member 3a,Wnt3a)基因遗传变异位点与崇仁麻鸡皮肤毛囊密度的相关性。本研究随机选取600只在相同环境和相同管理水平下饲养至上市日龄(120 d),健康且状况良好的崇仁麻鸡,其中原种母鸡20...旨在基于基因多态性分析Wnt家族成员3a(wnt family member 3a,Wnt3a)基因遗传变异位点与崇仁麻鸡皮肤毛囊密度的相关性。本研究随机选取600只在相同环境和相同管理水平下饲养至上市日龄(120 d),健康且状况良好的崇仁麻鸡,其中原种母鸡200只,公鸡100只和B系母鸡200只,公鸡100只。屠宰后选取背部、胸部和大腿部皮肤相同的位置测量毛囊密度,通过皮肤组织切片HE染色的方法观察毛囊的形态结构并测量毛囊直径和皮肤厚度。采血提DNA用PCR扩增后直接测序的方法筛选Wnt3a基因遗传变异位点,并与鸡皮肤毛囊密度进行相关性分析。通过实时荧光定量PCR技术检测Wnt3a基因在不同皮肤部位的表达量。结果表明:1)原种和B系同性别同皮肤部位相比较,原种母鸡背部毛囊密度极显著高于B系(P<0.01),大腿部毛囊密度显著高于B系(P<0.05),背部和大腿部毛囊直径显著低于B系(P<0.05)。原种公鸡背部皮肤毛囊密度极显著高于B系(P<0.01),胸部毛囊密度极显著低于B系(P<0.01),背部和大腿部毛囊直径显著低于B系(P<0.05)。B系公鸡胸部皮肤厚度显著高于原种(P<0.05),B系公、母鸡大腿皮肤厚度均显著高于原种(P<0.05)。2)Wnt3a基因筛选出的3个SNPs位点与各部位皮肤毛囊密度进行相关性分析后发现,g.2555812 T>C和g.2555377 T>C位点的CC基因型个体背部皮肤毛囊密度显著高于其他基因型个体背部(P<0.05)。3)实时荧光定量PCR结果显示,Wnt3a基因在原种和B系鸡的3个皮肤部位的毛囊组织上都有比较高的表达量,且在原种背部的表达量显著高于在B系背部的表达量(P<0.05)。综上所述,崇仁麻鸡原种皮肤毛孔相较于B系更加细密,Wnt3a基因g.2555812 T>C位点的CC基因型和g.2555377 T>C位点的CC基因型是崇仁麻鸡皮肤毛囊的有利基因型,可选作鸡皮肤毛囊密度性状的分子标记。本研究探讨了作为毛囊生长发育的候选基因Wnt3a的遗传效应及SNPs与皮肤毛囊性状的相关性,以期为优质鸡胴体包装性状的分子标记筛选与标记辅助选择提供参考依据。展开更多
为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的...为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的体重、体高、体厚、眼径、体长和背鳍前长6个生长性状进行QTL定位分析。结果显示,多鳞[鱼喜]首张高密度遗传连锁图谱全长2 154.803 c M,标记间平均遗传距离0.455 c M,共有4 735个SNP标记分配到24个连锁群。QTL定位分析结果发现在6个生长性状中共检测到20个生长显著相关QTL位点,分布在8个连锁群上,单个QTL的LOD值范围为3.02~4.23,可解释的表型变异范围为0.14%~8.42%。其中,在连锁群LG08聚集了8个生长性状显著相关的QTL。通过对候选QTL区间内的基因进行功能注释,共筛选到了19个潜在生长调控相关基因,包含igf1、igf2、sstr5、sst1a、tgfbr2、gas1、igfals、gfg6、gfg20、bmp7、kdm5c、tti1以及rbm10等。实验获得的遗传标记及相关候选基因是多鳞[鱼喜]生长相关性状标记辅助选择(MAS)的有用基因资源,为进一步研究鱼类生长调控机制提供了更多的理论依据。展开更多
文摘The authors utilized the polymerase chain reaction to rapidly and accurately type a variable number of tandemly repeated loci (VNTR loci) of the 3’ flanking region of apolipoprotein B (apo B) gene, and studied the relationship between this loci polymorphisms and coronary heart disease (CAD); comparisons were made with relative diseases and
基金the National Natural science Fundationof china(39970386)
文摘The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.
基金This work was supported by the National 973 Program(2004CB7206)National 863 Program of China(2002AA211052)Jiangsu Provincial Natural Science Foundation(BK2004045).
文摘Brachytic stem is a major trait in plant type .of soybean and its yield potential may be higher under high population when compared with normal stem. In the present investigation, 152 recombinant inbred line （RIL） families derived from the cross of Bogao （normal stem） and Nannong 94-156 （brachytic stem） were used to map genes and QTLs of three plant type traits and to identify the effects of brachytic stem on agronomic traits such as yield. The primary results indicated that brachytic stem （sb） and determinate growth habit （drl） were mapped on linkage groups B2 and L, three major QTLs related to plant height were detected and mapped on linkage group L near drl, another minor QTL was mapped near sb on linkage group B2-1. Lines with brachytic stem had shorter plant height, lower biomass, yield, harvest index and pods per plant, and essentially no differences in days to maturity and 100-seed weight when compared with normal stem lines. It was obvious that the effect of brachytic stem on yield was due to the decreased height, biomass and harvest index.
基金funded by the National Key Research and Development Program of China(2016YFD0100301)the National Natural Science Foundation of China(31671602)the Agricultural Science and Technology Innovation Program and the Cooperation and Innovation Mission(CAASZDXT2018001)
文摘The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.
文摘旨在基于基因多态性分析Wnt家族成员3a(wnt family member 3a,Wnt3a)基因遗传变异位点与崇仁麻鸡皮肤毛囊密度的相关性。本研究随机选取600只在相同环境和相同管理水平下饲养至上市日龄(120 d),健康且状况良好的崇仁麻鸡,其中原种母鸡200只,公鸡100只和B系母鸡200只,公鸡100只。屠宰后选取背部、胸部和大腿部皮肤相同的位置测量毛囊密度,通过皮肤组织切片HE染色的方法观察毛囊的形态结构并测量毛囊直径和皮肤厚度。采血提DNA用PCR扩增后直接测序的方法筛选Wnt3a基因遗传变异位点,并与鸡皮肤毛囊密度进行相关性分析。通过实时荧光定量PCR技术检测Wnt3a基因在不同皮肤部位的表达量。结果表明:1)原种和B系同性别同皮肤部位相比较,原种母鸡背部毛囊密度极显著高于B系(P<0.01),大腿部毛囊密度显著高于B系(P<0.05),背部和大腿部毛囊直径显著低于B系(P<0.05)。原种公鸡背部皮肤毛囊密度极显著高于B系(P<0.01),胸部毛囊密度极显著低于B系(P<0.01),背部和大腿部毛囊直径显著低于B系(P<0.05)。B系公鸡胸部皮肤厚度显著高于原种(P<0.05),B系公、母鸡大腿皮肤厚度均显著高于原种(P<0.05)。2)Wnt3a基因筛选出的3个SNPs位点与各部位皮肤毛囊密度进行相关性分析后发现,g.2555812 T>C和g.2555377 T>C位点的CC基因型个体背部皮肤毛囊密度显著高于其他基因型个体背部(P<0.05)。3)实时荧光定量PCR结果显示,Wnt3a基因在原种和B系鸡的3个皮肤部位的毛囊组织上都有比较高的表达量,且在原种背部的表达量显著高于在B系背部的表达量(P<0.05)。综上所述,崇仁麻鸡原种皮肤毛孔相较于B系更加细密,Wnt3a基因g.2555812 T>C位点的CC基因型和g.2555377 T>C位点的CC基因型是崇仁麻鸡皮肤毛囊的有利基因型,可选作鸡皮肤毛囊密度性状的分子标记。本研究探讨了作为毛囊生长发育的候选基因Wnt3a的遗传效应及SNPs与皮肤毛囊性状的相关性,以期为优质鸡胴体包装性状的分子标记筛选与标记辅助选择提供参考依据。
文摘为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的体重、体高、体厚、眼径、体长和背鳍前长6个生长性状进行QTL定位分析。结果显示,多鳞[鱼喜]首张高密度遗传连锁图谱全长2 154.803 c M,标记间平均遗传距离0.455 c M,共有4 735个SNP标记分配到24个连锁群。QTL定位分析结果发现在6个生长性状中共检测到20个生长显著相关QTL位点,分布在8个连锁群上,单个QTL的LOD值范围为3.02~4.23,可解释的表型变异范围为0.14%~8.42%。其中,在连锁群LG08聚集了8个生长性状显著相关的QTL。通过对候选QTL区间内的基因进行功能注释,共筛选到了19个潜在生长调控相关基因,包含igf1、igf2、sstr5、sst1a、tgfbr2、gas1、igfals、gfg6、gfg20、bmp7、kdm5c、tti1以及rbm10等。实验获得的遗传标记及相关候选基因是多鳞[鱼喜]生长相关性状标记辅助选择(MAS)的有用基因资源,为进一步研究鱼类生长调控机制提供了更多的理论依据。
