Clinical efficacy observation on treatment of primary dysmenorrhea with ginger-partitioned moxibustion at Zigong(EX-CA 1)

Objective： To investigate the clinical effect of ginger-partitioned moxibustion at Zigong（EX-CA 1） for primary dysmenorrhea. Methods： A total of 112 patients with primary dysmenorrhea were randomized into an observation group and a control group according to their visiting sequence, 56 cases in each group. Patients in the observation group received ginger-partitioned moxibustion at Zigong（EX-CA 1）, while patients in the control group received oral intake of analgesic. For both groups, treatment started 1 week before menstruation and lasted for 3 menstrual cycles, continued by a 3-month follow-up visit, then the clinical efficacy was evaluated. Results： By the end of treatment, symptom score in the observation group was lower than that in the control group, showing a statistical significance（P〈0.05）. After 3 months of treatment, the value of prostaglandin F2 a（PGF2α）, systolic-to-diastolic peak velocity ratio（S/D）, resistance index（RI） and pulsatility index（PI） in the observation group were significantly higher than those in the control group, showing statistical significances（all P〈0.01）.The recovery rate in the observation group was higher than that in the control group, showing a statistical significance（P〈0.05）. Conclusion： Ginger-partitioned moxibustion at Zigong（EX-CA 1） for primary dysmenorrhea is a combination of the merits of warming function of moxibustion, dissipating function of ginger and stimulation of acupoint, and is better than oral intake of analgesic.

Effect of ginger-partitioned moxibustion on immunocytokines in patients with chronic nonbacterial prostatitis

Objective： To observe the effect of ginger-partitioned moxibustion on immunocytokines in patients with chronic nonbacterial prostatitis （CNP）. Methods： A total of 80 CNP patients were randomly allocated into two groups according to their visiting sequence, 40 cases in each group. Cases in the observation group were treated with oral Tamsulosin Hydrochloride Sustained Release Capsules （Harnal） （0.2 mg for each dose, one dose a day） and ginger-partitioned moxibustion at Qihai （CV 6）, Guanyuan （CV 4）, Zhongji （CV 3） and bilateral Zusanli （ST 36）, Sanyinjiao （SP 6）, Pangguangshu （BL 28）, Shangliao （BL 31）, Ciliao （BL 32）, Zhongliao （BL 33） and Xialiao （BL 34） （once a day）. Cases in the control group were treated with the oral Western medication alone （same administration as those in the observation group）. Cases in both groups were treated for 28 d. Before and after treatment, the CD3＋, CD4＋, CDB＋, CD4＋ CD25＋, CD4＋ CD25＋ Foxp3＋, transforming growth factor （TGF）-β1, immunoglobulin （Ig） A, IgE, IgG and IgM were detected and scored using National Institutes of Health chronic prostatitis symptom index （NIH-CPSI）. Results： The total effective rate was 90.0% in the observation group, versus 72.5% in the control group, showing a statistical difference （P〈0.05）. After treatment, the CD3＋, CD4＋, CD8＋, CD4＋ CD25＋, CD4＋ CD25＋ Foxp3＋, TGF-131, IgA, IgG and IgM were significantly increased in both groups, showing statistical differences （P〈0.05）. There were between-group statistical differences in CD3＋, CD4＋, CD8＋, CD4＋ CD25＋, CD4＋ CD25＋ Foxp3＋, TGF-131, IgA, IgG, IgE, IgM, total NIH-CPSI score and pain and discomfort score （P〈0.05）. Conclusion： Ginger-partitioned moxibustion can improve clinical symptoms of CNP patients by improving their immune function.

Clinical observation on ginger-partitioned moxibustion plus manual repositioning for benign paroxysmal positional vertigo(BPPV)

Objective： To investigate the clinical effect of ginger-partitioned moxibustion combined with manual repositioning for benign paroxysmal positional vertigo（BPPV）. Methods： A total of 76 BPPV cases were randomly allocated into an observation group（n=38） and a control group（n=38）. Patients in the observation group received ginger-partitioned moxibustion at Tinggong（SI 19） plus manual repositioning, whereas patients in the control group received the same manual repositioning alone. Results： After 48 h of treatment, the total effective rate was 94.7% in the observation group, versus 86.8% in the control group; after 7 d of treatment, the total effective rate was 78.9% in the observation group, versus 73.7% in the control group, both showing between-group statistical differences（P〈0.05）. The follow-up after 3 months showed that 2 cases（6.7%） got relapse in the observation group, versus 8 cases（28.6%） in the control group, showing a statistical difference（P〈0.05）. The adverse reaction rate was 2.6% in the observation group, versus 21.1% in the control group, showing a statistical difference（P〈0.05）. Conclusion： Ginger-partitioned moxibustion at Tinggong（SI 19） plus manual repositioning can obtain better effect for BPPV than manual repositioning alone. In addition, this therapy has stable efficacy and causes less adverse reactions.

Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1,mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome

To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 （TFF1） and mucin 5AC （MUCSAC） levels, as well as the expression of epidermal growth factor receptor （EGFR） in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome. Methods： Seventy-five SPF grade Sprague-Dawley （SO） rats were randomly divided into a blank control group （group A）, a model group （group B）, a 3 moxa-cone ginger-partitioned moxibustion group （group C1）, a 6 moxa-cone ginger-partitioned moxibustion group （group C2） and a 9 moxa-cone ginger-partitioned moxibustion group （group C3） using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 ~C 200% concentrated Da Huang （Radix et RhizomaRhei） to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli （ST 36）and Zhongwan （CV 12） respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry. Results： After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased （all P〈0.01）; compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased （all P〈0.01）. Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased （all P〈0.01）, however, there was no significant difference between group C2 and C3 （all P〉0.05）. The mechanism may be related to the increase of serum TFF2 and MUC5AC levels and activation of EGFR protein. Conclusion： Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone Ringer-Dartitioned moxibustion groups.

Effects of ginger-partitioned moxibustion on the expression levels of PGF2α, E2, P, and mRNAs of PGF2αR and E2R in rats with primary dysmenorrhea due to cold-dampness stagnation

Objective To observe the effects of ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4)on the expression levels of endocrine-related molecules and their receptors in rats with primary dysmenorrhea(PD)due to cold-dampness stagnation,thus to explore their analgesic mechanisms.Methods Thirty-two female Wistar rats were divided into a normal group,a model group,a ginger-partitioned moxibustion group,and a Western medicine group according to the random number table method,with 8 rats in each group.Except for rats in the normal group,all other rats were treated with oxytocin combined with ice-water bath to establish the rat models of PD due to cold-dampness stagnation.After successful modeling,rats in the normal group and the model group did not receive treatment;rats in the ginger-partitioned moxibustion group received treatments with ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4);rats in the Western medicine group received ibuprofen by intragastric administration.The writhing response of rats was compared among groups,and the serum levels of prostaglandin F2α(PGF2α),estrogen(estradiol,E2),progesterone(P),and the mRNA expression of PGF2αand E2 receptors in the uterine tissues were detected.Results No writhing behavior was observed in the normal group;compared with the normal group,the serum PGF2αand E2 levels in the model group were increased(P<0.01),while the P level was decreased(P<0.01),and the mRNA expression levels of the uterine PGF2αand E2 receptors were increased(P<0.01,P<0.05).Compared with the model group,the writhing behavior latency was prolonged,and the writhing response score was decreased in the ginger-partitioned moxibustion group and the Western medicine group(P<0.01);the serum PGF2αand E2 levels in the ginger-partitioned moxibustion group and the Western medicine group were decreased,while the P level was increased(P<0.05 or P<0.01);the mRNA expression levels of the uterine PGF2αand E2 receptors in the ginger-partitioned moxibustion group and the Western medicine group were decreased(P<0.05).Compared with the Western medicine group,the ginger-partitioned moxibustion group showed a prolonged writhing behavior latency,reduced writhing response score(P<0.05),and decreased serum E2 level(P<0.05),while no statistical differences in the serum PGF2αand P levels,or the mRNA expression levels of uterine PGF2αand E2 receptors(P>0.05).Conclusion The analgesic effect of ginger-partitioned moxibustion on PD due to cold-dampness stagnation may be related to regulating the mRNA expression levels of PGF2αand E2 receptors in the uterine tissues.

Ginger-partitioned moxibustion plus pediatric massage for treating infantile diarrhea due to spleen deficiency:a randomized controlled clinical trial

Objective:To observe the clinical efficacy of ginger-partitioned moxibustion plus pediatric massage(tuina)in treating infantile diarrhea due to spleen deficiency.Methods:Ninety infants were randomly divided into a massage plus moxibustion group,a massage group and a drug group by the random number table method,with 30 cases in each group.The intervention was conducted for two consecutive courses.The infants in the massage plus moxibustion group were treated with pediatric massage and ginger-partitioned moxibustion at Shenque(CV 8).The infants in the massage group were treated with pediatric massage alone,while those in the drug group were treated with smecta.The primary and secondary symptom scales were assessed before and after treatment and at the follow-ups,and the total effective rate was evaluated after treatment.Results:The total effective rate in the massage plus moxibustion group was significantly different from that in the massage group and drug group(both P<0.05).After treatment,the scores of primary and secondary symptoms decreased in all three groups,with statistically significant intra-group differences(all P<0.05);the scores of primary symptoms were significantly different between the massage plus moxibustion group and the drug group(P<0.05);the scores of secondary symptoms in the massage plus moxibustion group and the massage group were significantly different from that in the drug group(both P<0.05).The differences in the time to recover normal bowel movement frequency among the three groups were not statistically significant(P>0.05).Conclusion:Ginger-partitioned moxibustion plus pediatric massage compared with pediatric massage or smecta monotherapy shows superior clinical efficacy in treating infantile diarrhea due to spleen deficiency,and has the advantages of appetite improvement,physique strengthening and short course.

Treatment of 28 Chronic Diarrhea Patients by Ginger-partitioned Moxibustion

Tianshu (ST 25), Shongwan (CV 12), Qihai (CV 6), Zusanli (ST 36) and etc were selected to treat chronic diarrhea. The curative effect between ginger moxibustion and acupuncture was compared. The effective rate of two groups was respectively 93% and 82%. Key Words Diarrhea, Chronic - Ginger-partitioned Moxibustion - Acupuncture-moxibustion Translator: WANG Si-you

Treatment of Child Diarrhea by Ginger-partitioned Moxibustion plus Tuina: A Report of 52 Cases

Treated 52 cases of child diarrhea with ginger-partitioned moxibustion plus Tuina, after three treatments, all the patients are cured. Key Words Point CV 8 (Shenque) - Ginger-partitioned Moxibustion - Massage - Tuina - Diarrhea - Abdominal Pain Author: LIU Xiao-feng (1968-), male, resident physician

Clinical Observation of 82 Cases of Enuresis Treated by Ginger-partitioned Moxibustion plus Cupping Therapy

Purpose: To observe the clinical therapeutic effect in the treatment of enuresis by ginger-partitioned moxibustion plus cupping therapy.Methods: Ginger-partitioned moxibustion was applied on Guanyuan (CV 4) and bilateral Zusanli (ST 36) and cupping therapy was applied on Shenque (CV 8) in the treatment of 82 cases of enuresis, in comparison with 76 cases treated by Chinese herbal medicine.Results: The effective rate was 84.1% in the treatment by ginger-partitioned moxibustion plus cupping therapy and was 64.5% in the treatment by Chinese herbal medicine.Conclusion: Ginger-partitioned moxibustion plus cupping therapy was better than Chinese herbal medicine in the treatment of enuresis (P<0.05). Key Words Ginger-partitioned Moxibustion - Cupping Therapy - Enuresis Translator: HUANG Guo-qi

Effects of moxibustion on the P2X7R/STAT3/VEGF pathway in rats with colitis-associated colon cancer

Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibustion intervening CACC through the purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)pathway.Methods:A total of 26 male Sprague-Dawley rats were selected.According to the random number table method,6 rats were selected as the normal group.The remaining 20 rats were injected intraperitoneally with azoxymethane(AOM)combined with oral dextran sodium sulfate(DSS)to prepare the CACC model.After the model was successfully established,2 rats were randomly selected for model identification.The remaining 18 rats which were successfully modeled were randomly divided into a model group,a herb-partitioned moxibustion group and a ginger-partitioned moxibustion group,with 6 rats in each group.Moxibustion intervention was performed in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group at Qihai(CV 6)and bilateral Tianshu(ST 25).Moxibustion was performed twice at each point each time,once a day,at a 1-day interval after 6 consecutive interventions,for a total of 30 interventions.After intervention,the colon tumor load,pathological change and histopathological score were observed.Immunohistochemistry was used to detect the expressions of VEGF,P2X7R,phospho-STAT3(p-STAT3),and nuclear factor-kappa B p65(NF-κB p65)proteins in rat colon tissue.Western blot was used to detect the levels of p-STAT3 and NF-κB p65 proteins in rat colon tissue.Results:Compared with the normal group,the colon tumor load and histopathological score in the model group were significantly increased(both P<0.001),and different grades of dysplasia were observed in colon tissue from the model group,reaching the degree of adenocarcinoma;the expression level of P2X7R protein in colon tissue was significantly decreased(P<0.001),and the expression levels of p-STAT3,NF-κB p65 and VEGF proteins were significantly increased(all P<0.001)in the model group.Compared with the model group,the colon tumor load,colon histopathological score and the levels of p-STAT3,NF-κB p65 and VEGF proteins in colon tissue were significantly decreased(all P<0.05)in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group while the expression levels of P2X7R protein in colon tissue were significantly increased(both P<0.05).Conclusion:Both herb-partitioned moxibustion and ginger-partitioned moxibustion can reduce the colon tumor load in CACC rats and delay the progression of colon adenomas.The mechanism may be mediated by the P2X7R/STAT3 pathway to inhibit STAT3 phosphorylation,thereby reducing VEGF protein expression.