In this paper,we first propose a memristive chaotic system and implement it by circuit simulation.The chaotic dynamics and various attractors are analysed by using phase portrait,bifurcation diagram,and Lyapunov expon...In this paper,we first propose a memristive chaotic system and implement it by circuit simulation.The chaotic dynamics and various attractors are analysed by using phase portrait,bifurcation diagram,and Lyapunov exponents.In particular,the system has robust chaos in a wide parameter range and the initial value space,which is favourable to the security communication application.Consequently,we further explore its application in image encryption and present a new scheme.Before image processing,the external key is protected by the Grain-128a algorithm and the initial values of the memristive system are updated with the plain image.We not only perform random pixel extraction and masking with the chaotic cipher,but also use them as control parameters for Brownian motion to obtain the permutation matrix.In addition,multiplication on the finite field GF(2^(8))is added to further enhance the cryptography.Finally,the simulation results verify that the proposed image encryption scheme has better performance and higher security,which can effectively resist various attacks.展开更多
目的·探究miR-128-3p/瘦素(leptin,LEP)轴对银屑病中角质形成细胞过度增殖及炎症反应的调控作用。方法·选取BALB/c小鼠,分为对照组(n=10)及模型组(n=10)。模型组小鼠背部行咪喹莫特软膏连续涂抹以构建银屑病小鼠模型。构建miR...目的·探究miR-128-3p/瘦素(leptin,LEP)轴对银屑病中角质形成细胞过度增殖及炎症反应的调控作用。方法·选取BALB/c小鼠,分为对照组(n=10)及模型组(n=10)。模型组小鼠背部行咪喹莫特软膏连续涂抹以构建银屑病小鼠模型。构建miR-128-3p过表达和干扰质粒、LEP干扰质粒分别转染人永生化角质形成细胞HaCaT细胞。经实时定量聚合酶链反应检测miR-128-3p及LEP的mRNA,Western blotting检测LEP蛋白表达;酶联免疫吸附实验检测培养液中肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、IL-1β的含量;MTT实验检测细胞相对活力;EdU实验检测细胞增殖;采用双荧光素酶报告基因实验验证miR-128-3p与LEP的靶向关系。结果·与对照组小鼠比,模型组小鼠miR-128-3p表达下调,Lep的基因表达及蛋白水平升高(均P<0.05)。双荧光素酶报告基因实验证实LEP是miR-128-3p下游靶点。与模拟物阴性对照(negative control of mimic,NC mimic)组相比,miR-128-3p模拟物(miR-128-3p mimic)组miR-128-3p表达上调,LEP的基因表达及蛋白降低;miR-128-3p mimic组TNF-α、IL-6、IL-1β显著低于NC mimic;miR-128-3p上调后细胞相对活力、EdU阳性细胞率均降低(均P<0.05)。与抑制物阴性对照(negative control of inhibitor,NC inhibitor)组相比,miR-128-3p抑制物(miR-128-3p inhibitor)组miR-128-3p表达下调,LEP的基因表达及蛋白水平增加;miR-128-3p下调后细胞TNF-α、IL-6、IL-1β升高;miR-128-3p表达下调导致细胞相对活力和EdU阳性细胞率增加(均P<0.05)。进一步实验结果显示:与LEP抑制物(LEP inhibitor)组相比,miR-128-3p inhibitor+LEP inhibitor组LEP表达上调,而TNF-α、IL-6、IL-1β水平升高,细胞相对活力及EdU阳性细胞率增加(均P<0.05)。结论·miR-128-3p靶向沉默LEP抑制角质形成细胞增殖及炎症反应,抑制银屑病发生发展。展开更多
基金This work was supported by the National Natural Science Foundation of China(61203004)the Natural Science Foundation of Heilongjiang Province(F201220)the Heilongjiang Provincial Natural Science Foundation of Joint Guidance Project(LH2020F022).
文摘In this paper,we first propose a memristive chaotic system and implement it by circuit simulation.The chaotic dynamics and various attractors are analysed by using phase portrait,bifurcation diagram,and Lyapunov exponents.In particular,the system has robust chaos in a wide parameter range and the initial value space,which is favourable to the security communication application.Consequently,we further explore its application in image encryption and present a new scheme.Before image processing,the external key is protected by the Grain-128a algorithm and the initial values of the memristive system are updated with the plain image.We not only perform random pixel extraction and masking with the chaotic cipher,but also use them as control parameters for Brownian motion to obtain the permutation matrix.In addition,multiplication on the finite field GF(2^(8))is added to further enhance the cryptography.Finally,the simulation results verify that the proposed image encryption scheme has better performance and higher security,which can effectively resist various attacks.
文摘目的·探究miR-128-3p/瘦素(leptin,LEP)轴对银屑病中角质形成细胞过度增殖及炎症反应的调控作用。方法·选取BALB/c小鼠,分为对照组(n=10)及模型组(n=10)。模型组小鼠背部行咪喹莫特软膏连续涂抹以构建银屑病小鼠模型。构建miR-128-3p过表达和干扰质粒、LEP干扰质粒分别转染人永生化角质形成细胞HaCaT细胞。经实时定量聚合酶链反应检测miR-128-3p及LEP的mRNA,Western blotting检测LEP蛋白表达;酶联免疫吸附实验检测培养液中肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、IL-1β的含量;MTT实验检测细胞相对活力;EdU实验检测细胞增殖;采用双荧光素酶报告基因实验验证miR-128-3p与LEP的靶向关系。结果·与对照组小鼠比,模型组小鼠miR-128-3p表达下调,Lep的基因表达及蛋白水平升高(均P<0.05)。双荧光素酶报告基因实验证实LEP是miR-128-3p下游靶点。与模拟物阴性对照(negative control of mimic,NC mimic)组相比,miR-128-3p模拟物(miR-128-3p mimic)组miR-128-3p表达上调,LEP的基因表达及蛋白降低;miR-128-3p mimic组TNF-α、IL-6、IL-1β显著低于NC mimic;miR-128-3p上调后细胞相对活力、EdU阳性细胞率均降低(均P<0.05)。与抑制物阴性对照(negative control of inhibitor,NC inhibitor)组相比,miR-128-3p抑制物(miR-128-3p inhibitor)组miR-128-3p表达下调,LEP的基因表达及蛋白水平增加;miR-128-3p下调后细胞TNF-α、IL-6、IL-1β升高;miR-128-3p表达下调导致细胞相对活力和EdU阳性细胞率增加(均P<0.05)。进一步实验结果显示:与LEP抑制物(LEP inhibitor)组相比,miR-128-3p inhibitor+LEP inhibitor组LEP表达上调,而TNF-α、IL-6、IL-1β水平升高,细胞相对活力及EdU阳性细胞率增加(均P<0.05)。结论·miR-128-3p靶向沉默LEP抑制角质形成细胞增殖及炎症反应,抑制银屑病发生发展。
