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Antioxidant metabolic system and comparative proteomics analysis in winter turnip rape(Brassica rapa L.)under cold stress
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作者 Xiucun Zeng Li Ma +6 位作者 Jinhai Yuan Yaping Xie Tianxia Guan Xiaoqin Wang Guotai Ma Yaozhao Xu Wancang Sun 《Oil Crop Science》 CSCD 2022年第2期95-102,共8页
Winter turnip rape(Brassica rapa L.)is widely cultivated in winter in Northwest China,however,its cold-tolerant mechanism remains insufficiently understood.In this study,winter turnip rape cultivar Longyou 7,a cold-to... Winter turnip rape(Brassica rapa L.)is widely cultivated in winter in Northwest China,however,its cold-tolerant mechanism remains insufficiently understood.In this study,winter turnip rape cultivar Longyou 7,a cold-tolerant variety,was used as material,whose accumulation of H_(2)O_(2) and O_(2)^(·-),antioxidant enzyme activity as well as differences in protein expression based on two-dimensional gel electrophoresis(2-DE)technique under -4℃ stress were analyzed.Results showed that,production of H_(2)O_(2) and O_(2)^(·-) were increased in Longyou 7 leaves,simultaneously,SOD and POD activities were also obviously rosed up,but the activities of CAT and APX were gradually reduced with the temperature.Thirty-six differential protein spots were successfully identified between control and treatments group by using mass spectrometry analysis.Among them,4 differential protein spots were induced under cold stress,and 2 were inhibited at-4℃.Functional analysis found that these identified proteins mainly participated in photosynthesis,carbohydrate metabolism,defense,protein synthesis,enzyme activity,redox and membrane metabolism,respectively.Additionally,13 proteins'function were still unknown.In conclusion,strong antioxidant capacity and cell defense ability might play important roles in Longyou 7 response to cold stress. 展开更多
关键词 Brassica rapa Accumulation of H2O2 and O2- Antioxidant enzyme activity Cold stress
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Enhanced anaerobic fermentation with azo dye as electron acceptor: Simultaneous acceleration of organics decomposition and azo decolorization
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作者 Yang Li Yaobin Zhang +3 位作者 Xie Quan Jingxin Zhang Shuo Chen Shahzad Afzal 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2014年第10期1970-1976,共7页
Accumulation of hydrogen during anaerobic processes usually results in low decomposition of volatile organic acids(VFAs). On the other hand, hydrogen is a good electron donor for dye reduction, which would help the ... Accumulation of hydrogen during anaerobic processes usually results in low decomposition of volatile organic acids(VFAs). On the other hand, hydrogen is a good electron donor for dye reduction, which would help the acetogenic conversion in keeping low hydrogen concentration. The main objective of the study was to accelerate VFA composition through using azo dye as electron acceptor. The results indicated that the azo dye serving as an electron acceptor could avoid H2 accumulation and accelerate anaerobic digestion of VFAs. After adding the azo dye, propionate decreased from 2400.0 to 689.5 mg/L and acetate production increased from 180.0 to 519.5 mg/L. It meant that the conversion of propionate into acetate was enhanced. Fluorescence in situ hybridization analysis showed that the abundance of propionate-utilizing acetogens with the presence of azo dye was greater than that in a reference without azo dye. The experiments via using glucose as the substrate further demonstrated that the VFA decomposition and the chemical oxygen demand(COD) removal increased by 319.7 mg/L and 23.3% respectively after adding the azo dye. Therefore, adding moderate azo dye might be a way to recover anaerobic system from deterioration due to the accumulation of H2 or VFAs. 展开更多
关键词 Azo dye Anaerobic treatment Volatile fatty acids h2accumulation
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