Helicobacter heilmannii sensu lato(H.heilmannii s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other.H.heilmannii s.l.infect the stomach of...Helicobacter heilmannii sensu lato(H.heilmannii s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other.H.heilmannii s.l.infect the stomach of several animals and may have zoonotic potential.Although the prevalence of these infections in humans is low,they are associated with gastric pathology,including mucosa-associated lymphoid tissue lymphoma,making them a significant health issue.Here,the taxonomy,epidemiology,microbiology,diagnosis,and treatment of these infections will be reviewed.The gastric pathology associated with H.heilmannii s.l.infections in humans will also be addressed.Finally,the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be reviewed.The understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute H.heilmannii s.l.is essential for developing strategies for prevention and treatment of these infections.展开更多
为了进一步阐明血小板生成素(thrombopoietin,Tpo)的生物学作用,我们研究了rhTpo(recombinant human Tpo)对人红白血病(human erythroleukemia,HEL)细胞增殖的影响。采用血浆凝块集落培养法和5-溴-2’-脱氧尿核苷(5-BrdU)掺入HEL细胞DNA...为了进一步阐明血小板生成素(thrombopoietin,Tpo)的生物学作用,我们研究了rhTpo(recombinant human Tpo)对人红白血病(human erythroleukemia,HEL)细胞增殖的影响。采用血浆凝块集落培养法和5-溴-2’-脱氧尿核苷(5-BrdU)掺入HEL细胞DNA-ELISA法。培养基中加入rhTpo,HEL细胞集落数量和吸光度值明显高于未加rhTPo对照组。当rhTPo浓度为10,50,100,200ng/ml时,实验组集落数分别是对照组集落数的1.5,2.07,2.34,2.46倍;实验组A值分别是对照组A值的1.28,1.84,2.25,2.31倍。研究结果表明,rhTpo促进HEL细胞增殖和DNA合成。展开更多
目的:探讨白菊花抗巨细胞病毒(CMV)诱导的HEL细胞凋亡作用及其可能的分子机制。方法:将HEL细胞分为正常对照组、CMV对照组和药物预处理+CMV作用组,选用三种不同浓度(10μM,20μM and 50μM)的药物预处理三种细胞4h,再以病毒感染各组细...目的:探讨白菊花抗巨细胞病毒(CMV)诱导的HEL细胞凋亡作用及其可能的分子机制。方法:将HEL细胞分为正常对照组、CMV对照组和药物预处理+CMV作用组,选用三种不同浓度(10μM,20μM and 50μM)的药物预处理三种细胞4h,再以病毒感染各组细胞诱导其凋亡,通过MTT法测定各组细胞的存活率,并用流式细胞仪通过Annexin V/PI双染法检测各组细胞的凋亡百分比,用Western blot检测各组细胞中凋亡相关蛋白Caspase-3、6、7、9和NF-κB的表达。结果:不同浓度的白菊花能够提高CMV诱导的HEL细胞的存活率,而且白菊花可降低CMV诱导的HEL细胞的凋亡百分比,其中尤以两种药物的高浓度组作用最为显著,呈现出明显的剂量依赖关系;Western blot检测显示白菊花降低Caspase-3、6、7、9和NF-κB的蛋白表达,且随着浓度的升高,蛋白的表达量表现出逐渐减少的趋势,其中以两种药物的高浓度作用组最为明显。结论:白菊花对CMV诱导的HEL细胞凋亡有不同程度的抑制作用,其机制可能与抑制细胞中凋亡相关蛋白Caspase-3、6、7、9和NF-κB信号传导通路的激活有关。展开更多
文摘Helicobacter heilmannii sensu lato(H.heilmannii s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other.H.heilmannii s.l.infect the stomach of several animals and may have zoonotic potential.Although the prevalence of these infections in humans is low,they are associated with gastric pathology,including mucosa-associated lymphoid tissue lymphoma,making them a significant health issue.Here,the taxonomy,epidemiology,microbiology,diagnosis,and treatment of these infections will be reviewed.The gastric pathology associated with H.heilmannii s.l.infections in humans will also be addressed.Finally,the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be reviewed.The understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute H.heilmannii s.l.is essential for developing strategies for prevention and treatment of these infections.
文摘为了进一步阐明血小板生成素(thrombopoietin,Tpo)的生物学作用,我们研究了rhTpo(recombinant human Tpo)对人红白血病(human erythroleukemia,HEL)细胞增殖的影响。采用血浆凝块集落培养法和5-溴-2’-脱氧尿核苷(5-BrdU)掺入HEL细胞DNA-ELISA法。培养基中加入rhTpo,HEL细胞集落数量和吸光度值明显高于未加rhTPo对照组。当rhTPo浓度为10,50,100,200ng/ml时,实验组集落数分别是对照组集落数的1.5,2.07,2.34,2.46倍;实验组A值分别是对照组A值的1.28,1.84,2.25,2.31倍。研究结果表明,rhTpo促进HEL细胞增殖和DNA合成。
