[Objective] The study aimed at predicting the T cell epitopes from proteins encoded by the specific RD1 region of Mycobacterium tuberculosis.[Method] By using bioinformatics software NetMHC Server,all possible peptide...[Objective] The study aimed at predicting the T cell epitopes from proteins encoded by the specific RD1 region of Mycobacterium tuberculosis.[Method] By using bioinformatics software NetMHC Server,all possible peptides sequences from RD1 region of M.tuberculosis were analyzed in silico for the ability to bind to 34 alleles of HLA I and 9 alleles of HLA II.[Result] 1 580 peptides binding to 9 alleles of HLA II and 336 peptides binding to 34 alleles of HLA I were obtained by predication.[Conclusion] Identification of T cell epitopes from the proteins encoded by RD1 region may serve to define candidate antigens with potentials in specific diagnosis and development of new vaccines against TB.展开更多
动物实验证实协同刺激分子CD80(B7-1)是促进抗肿瘤免疫应答的重要分子.本文应用RT-PCR,FACS技术检测了人肿瘤细胞系及EBV转化的B细胞CD80的表达,结果表明除Raji细胞、EBV转化的B细胞CD80阳性外,3AO、MCF-7、MDA-453、MKN-45、Hela细胞...动物实验证实协同刺激分子CD80(B7-1)是促进抗肿瘤免疫应答的重要分子.本文应用RT-PCR,FACS技术检测了人肿瘤细胞系及EBV转化的B细胞CD80的表达,结果表明除Raji细胞、EBV转化的B细胞CD80阳性外,3AO、MCF-7、MDA-453、MKN-45、Hela细胞均为阴性.用我室建立的CD80逆转录病毒载体转染包装细胞PA317,筛选高滴度病毒上情感染人肿瘤细胞,得到CD80阳性表达的肿瘤细胞克隆.利用转染前后的人乳腺癌细胞系MDA-453作ICAM-I、HLA class Ⅰ、classⅡ分子表达的分析,结果表明转染有CD80基因的MDA-453细胞ICAM-Ⅰ、HLA classⅠ分子表达上调,class Ⅱ分子的表达未见改变.展开更多
基金Supported by National Science and Technology Major Project(2008ZX10301)~~
文摘[Objective] The study aimed at predicting the T cell epitopes from proteins encoded by the specific RD1 region of Mycobacterium tuberculosis.[Method] By using bioinformatics software NetMHC Server,all possible peptides sequences from RD1 region of M.tuberculosis were analyzed in silico for the ability to bind to 34 alleles of HLA I and 9 alleles of HLA II.[Result] 1 580 peptides binding to 9 alleles of HLA II and 336 peptides binding to 34 alleles of HLA I were obtained by predication.[Conclusion] Identification of T cell epitopes from the proteins encoded by RD1 region may serve to define candidate antigens with potentials in specific diagnosis and development of new vaccines against TB.
文摘动物实验证实协同刺激分子CD80(B7-1)是促进抗肿瘤免疫应答的重要分子.本文应用RT-PCR,FACS技术检测了人肿瘤细胞系及EBV转化的B细胞CD80的表达,结果表明除Raji细胞、EBV转化的B细胞CD80阳性外,3AO、MCF-7、MDA-453、MKN-45、Hela细胞均为阴性.用我室建立的CD80逆转录病毒载体转染包装细胞PA317,筛选高滴度病毒上情感染人肿瘤细胞,得到CD80阳性表达的肿瘤细胞克隆.利用转染前后的人乳腺癌细胞系MDA-453作ICAM-I、HLA class Ⅰ、classⅡ分子表达的分析,结果表明转染有CD80基因的MDA-453细胞ICAM-Ⅰ、HLA classⅠ分子表达上调,class Ⅱ分子的表达未见改变.
