高迁移率蛋白B1(high mobility group protein box 1,HMGB1)为高迁移率族(high mobility group,HMG)成员之一,存在于真核生物细胞内。HMGB1不仅是一种核内蛋白,也是一种促炎因子,体内HMGB1升高往往提示细胞破坏或炎症反应。各种原因所...高迁移率蛋白B1(high mobility group protein box 1,HMGB1)为高迁移率族(high mobility group,HMG)成员之一,存在于真核生物细胞内。HMGB1不仅是一种核内蛋白,也是一种促炎因子,体内HMGB1升高往往提示细胞破坏或炎症反应。各种原因所致的肝损伤可导致HMGB1释放,HMGB1含量不仅对肝损伤的诊断有提示作用,对其预后也有一定的预测作用。研究HMGB1对肝损伤的作用机制,对治疗靶点有一定的指导意义。本文对HMGB1参与肝损伤的作用机制、与肝损伤的关系、预后分析、治疗靶点研究及未来应用前景予以综述。展开更多
The word "autophagy" is derived from the Greek roots "auto" and "phagy" which mean "self" and "to eat", respectively.As a hot topic and rapidly expanding field in biol...The word "autophagy" is derived from the Greek roots "auto" and "phagy" which mean "self" and "to eat", respectively.As a hot topic and rapidly expanding field in biology and medicine, autophagy is a process by which cytoplasmic components, including soluble macromolecules (such as nucleic acids, proteins, carbohydrates and lipids) and organelles (such as mitochondria, peroxisomes and endoplasmic reticulum) are degraded by lysosomes.There are at least three recognized types of autophagy including chaperone-mediated autophagy, microautophagy, and macroautophagy[1].展开更多
目的探索缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)与母亲怀孕年龄及血清中高迁移率蛋白-1(high mobility group box protein 1,hmgb1)水平相关性,为有效预防和减少HIE的发生提供依据。方法随机选取山东大学第二医院于2012...目的探索缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)与母亲怀孕年龄及血清中高迁移率蛋白-1(high mobility group box protein 1,hmgb1)水平相关性,为有效预防和减少HIE的发生提供依据。方法随机选取山东大学第二医院于2012年3月—2014年3月收治的住院患者60例为病例组,同期60名正常新生儿为对照组。采用酶联免疫-双抗体夹心法测定病例组和对照组的血清hmgb1水平,并记录其孕母年龄,进行统计学分析。结果病例组母亲怀孕平均年龄较大,为(33.5±4.7)岁,高龄产妇的比例明显高于对照组;出生后2h内病例组各种程度HIE患儿(重、中、轻)的血清hmgb1水平均高于对照组,病情越严重,hmgb1在血清中的含量越高(174.88±9.12)ng/m L,且恢复正常的时间越长。结论 HIE与母亲怀孕年龄及血清中hmgb1水平相关,高龄产妇易导致HIE,hmgb1是早期临床检测HIE病情严重程度的指标之一。展开更多
Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male...Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male Sprague-Dawley rats were randomly divided into three groups： sham group, TBI group, and TBI＋Gly group （n=36 per group）. Rat TBI model was made by using the modified Feeney＇s method. In TBI＋Gly group, Gly was administered intravenously at a dosage of l0 mg/kg 30 min after TBI. At 24 h after TBI, motor function and brain water content were evaluated. Meanwhile, HMGB 1/HMGB 1 receptors including toll-like receptor 4 （TLR4） and receptor for advanced glycation end products （RAGE）/nuclear fac- tor-κ B（NF- κ B） signaling pathway and inflammatory cytokines in the injured brain tissues were detected using quantitative real-time polymerase chain reaction, western blot, electrophoretic mobility shift assay and enzyme-linked immunosorbent assay. Furthermore, HMGB l, RAGE and TLR4 immunohistochemistry and apoptosis were analyzed. Results： Beam walking performance impairment and brain edema were significantly reduced in TBI＋Gly group compared with TBI group; meanwhile, the over-expressions of HMGB 1PHMGB 1 receptors （TLR4 and RAGE）/NF- κB DNA-binding activity and inflammatory cytokines were inhibited. The percentages of HMGB 1, RAGE and TLR4- positive cells and apoptotic cells were respectively 58.37%±5.06%, 54.15%±4.65%, 65.50%± 4.83%, 52.02%±4.63% in TBI group and 39.99%±4.99%, 34.87%±5.02%, 43.33%±4.54%, 37.84%±5.16% in TBI＋Gly group （all P〈0.01 compared with TBI group）. Conclusion： Gly can reduce secondary brain injury and improve outcomes in rat following TBI by down-regula- tion of HMGB 1/HMGB 1 receptors （TLR4 and RAGE）/NF- κB - mediated inflammatory responses in the injured rat brain.展开更多
文摘高迁移率蛋白B1(high mobility group protein box 1,HMGB1)为高迁移率族(high mobility group,HMG)成员之一,存在于真核生物细胞内。HMGB1不仅是一种核内蛋白,也是一种促炎因子,体内HMGB1升高往往提示细胞破坏或炎症反应。各种原因所致的肝损伤可导致HMGB1释放,HMGB1含量不仅对肝损伤的诊断有提示作用,对其预后也有一定的预测作用。研究HMGB1对肝损伤的作用机制,对治疗靶点有一定的指导意义。本文对HMGB1参与肝损伤的作用机制、与肝损伤的关系、预后分析、治疗靶点研究及未来应用前景予以综述。
文摘The word "autophagy" is derived from the Greek roots "auto" and "phagy" which mean "self" and "to eat", respectively.As a hot topic and rapidly expanding field in biology and medicine, autophagy is a process by which cytoplasmic components, including soluble macromolecules (such as nucleic acids, proteins, carbohydrates and lipids) and organelles (such as mitochondria, peroxisomes and endoplasmic reticulum) are degraded by lysosomes.There are at least three recognized types of autophagy including chaperone-mediated autophagy, microautophagy, and macroautophagy[1].
文摘目的探索缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)与母亲怀孕年龄及血清中高迁移率蛋白-1(high mobility group box protein 1,hmgb1)水平相关性,为有效预防和减少HIE的发生提供依据。方法随机选取山东大学第二医院于2012年3月—2014年3月收治的住院患者60例为病例组,同期60名正常新生儿为对照组。采用酶联免疫-双抗体夹心法测定病例组和对照组的血清hmgb1水平,并记录其孕母年龄,进行统计学分析。结果病例组母亲怀孕平均年龄较大,为(33.5±4.7)岁,高龄产妇的比例明显高于对照组;出生后2h内病例组各种程度HIE患儿(重、中、轻)的血清hmgb1水平均高于对照组,病情越严重,hmgb1在血清中的含量越高(174.88±9.12)ng/m L,且恢复正常的时间越长。结论 HIE与母亲怀孕年龄及血清中hmgb1水平相关,高龄产妇易导致HIE,hmgb1是早期临床检测HIE病情严重程度的指标之一。
文摘Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male Sprague-Dawley rats were randomly divided into three groups： sham group, TBI group, and TBI＋Gly group （n=36 per group）. Rat TBI model was made by using the modified Feeney＇s method. In TBI＋Gly group, Gly was administered intravenously at a dosage of l0 mg/kg 30 min after TBI. At 24 h after TBI, motor function and brain water content were evaluated. Meanwhile, HMGB 1/HMGB 1 receptors including toll-like receptor 4 （TLR4） and receptor for advanced glycation end products （RAGE）/nuclear fac- tor-κ B（NF- κ B） signaling pathway and inflammatory cytokines in the injured brain tissues were detected using quantitative real-time polymerase chain reaction, western blot, electrophoretic mobility shift assay and enzyme-linked immunosorbent assay. Furthermore, HMGB l, RAGE and TLR4 immunohistochemistry and apoptosis were analyzed. Results： Beam walking performance impairment and brain edema were significantly reduced in TBI＋Gly group compared with TBI group; meanwhile, the over-expressions of HMGB 1PHMGB 1 receptors （TLR4 and RAGE）/NF- κB DNA-binding activity and inflammatory cytokines were inhibited. The percentages of HMGB 1, RAGE and TLR4- positive cells and apoptotic cells were respectively 58.37%±5.06%, 54.15%±4.65%, 65.50%± 4.83%, 52.02%±4.63% in TBI group and 39.99%±4.99%, 34.87%±5.02%, 43.33%±4.54%, 37.84%±5.16% in TBI＋Gly group （all P〈0.01 compared with TBI group）. Conclusion： Gly can reduce secondary brain injury and improve outcomes in rat following TBI by down-regula- tion of HMGB 1/HMGB 1 receptors （TLR4 and RAGE）/NF- κB - mediated inflammatory responses in the injured rat brain.
