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Recurrent malignant hyperthermia after scoliosis correction surgery
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作者 Junfeng Su Man Huang 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2024年第1期70-72,共3页
Malignant hyperthermia(MH)is a genetic disorder of skeletal muscle cells that affects muscle cytoplasmic calcium homeostasis,with high mortality and low morbidity.Generally,it presents with non-specific signs of a hyp... Malignant hyperthermia(MH)is a genetic disorder of skeletal muscle cells that affects muscle cytoplasmic calcium homeostasis,with high mortality and low morbidity.Generally,it presents with non-specific signs of a hypermetabolic response,including high fever,tachycardia,and elevated end-tidal carbon dioxide(ETCO_(2)).The successful treatment lies in the timely recognition and early use of dantrolene.[1]As an inhibitor of Ca2+release through ryanodine receptor(RYR)channels,the skeletal muscle relaxant dantrolene has proven to be both a valuable experimental probe of intracellular Ca2+signaling and a lifesaving treatment for MH.[2]Dominant mutations in the skeletal muscle RYR1 gene are well-recognized causes of both malignant hyperthermia susceptibility(MHS)and central core disease(CCD). 展开更多
关键词 hyperthermia MALIGNANT ELEVATED
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Structural, Magnetic and Heating Efficiency of Ball Milled γ-Fe2O3/Gd2O3 Nanocomposite for Magnetic Hyperthermia
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作者 Abdelaziz Sabik 《Advances in Materials Physics and Chemistry》 CAS 2024年第1期15-23,共9页
The preparation of γ-Fe<sub>2</sub>O<sub>3</sub>/Gd<sub>2</sub>O<sub>3</sub> nanocomposite for possible use in magnetic hyperthermia application was done by ball millin... The preparation of γ-Fe<sub>2</sub>O<sub>3</sub>/Gd<sub>2</sub>O<sub>3</sub> nanocomposite for possible use in magnetic hyperthermia application was done by ball milling technique. The nanocomposite was characterized by X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The heating efficiency and the effect of milling time (5 h and 30 h) on the structural and magnetic properties of the nanocomposite were reported. XRD analysis confirms the formation of the nanocomposite, while magnetization measurements show that the milled sample present hysteresis with low coercivity and remanence. The specific absorption rate (SAR) under an alternating magnetic field is investigated as a function of the milling time. A mean heating efficiency of 68 W/g and 28.7 W/g are obtained for 5 h and 30 h milling times respectively at 332 kHz and 170 Oe. The results showed that the obtained nanocomposite for 5 h milling time is a promising candidate for magnetic hyperthermia due to his properties which show an interesting magnetic behavior and high specific absorption rate. 展开更多
关键词 Magnetic hyperthermia Iron Oxides Nanoparticles Maghemite Ball Milling XRD VSM
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Preclinical Verification of Modulated Electro-Hyperthermia —Part I. In Vitro Research
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作者 Andras Szasz 《International Journal of Clinical Medicine》 CAS 2024年第7期257-298,共42页
Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency mo... Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency modulation. The high-frequency part was chosen to select the malignant lesion using the specialties of the tumor: the higher conductivity and dielectric constant of the tumor than its host. The electric field selects the tumor, and the low-frequency amplitude modulation polarizes and excites the transmembrane proteins of the malignant cells. The dominant absorption of the energy by the microscopic clusters of the membrane rafts acts like nanoparticle heating. Exciting the membrane produces various apoptotic signals. The processes were modeled using silico and phantom experiments, which proved the concept. The preclinical verification was made in vitro and in vivo, and in the end, clinical proofs validated the method. Our objective is to follow all the development steps from the laboratory to the clinics in a trilogy of articles. This present is the first part, which deals with in silico, phantom, and in vitro research. 展开更多
关键词 Modulated Electro-hyperthermia mEHT In Silico Calculations Phantom Measurement In Vitro Experiments Thermal Effects Nonthermal Processes Apoptosis
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Calculation of Electromagnetic Field and SAR Dis- tributions of Two Antenna Structures for Bone Tu- mors Microwave Hyperthermia 被引量:1
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作者 席晓莉 汪文秉 王丽黎 《Journal of Nanjing Medical University》 2003年第3期143-148,共6页
Objective: To investigate the electromagnetic field and specific absorptionrate (SAR) distribution of different structure applicators with different depths for treating bonetumors using microwave hyperthermia. Methods... Objective: To investigate the electromagnetic field and specific absorptionrate (SAR) distribution of different structure applicators with different depths for treating bonetumors using microwave hyperthermia. Methods: The finite element method (FEM) was used to calculate,electromagnetic field and SAR distribution. Two different structure applicators were simulated. Theone is simple coaxial antenna, which has been successfully used in clinic treating bone tumors inTangdu hospital of the Forth Military Medical University several years. It was formed by a coaxialcable peeled off the out copper at end. The other applicator was coaxial- slot antenna, which waswidely used in microwave hyperthermia. The applicator inserted into the cylindrical bone withdifferent depths, and worked at the frequency of 2 450 MHz. Results: The electric field and SARgenerated by the simple coaxial applicator were mainly concentrated out the tissues, and were notuniform in the tissues, while the coaxial- slot applicator well transmits the electric field and SARinto the tissues, and can easily treat different position by adjusting the slat position.Conclusion: The results calculated by EFM, were well accordant with the experimental and clinicalresulls, and will be important for improving the clinical effects of microwave hyperthermia. 展开更多
关键词 bone tumors microwave hyperthermia finite element method electromagnetic field specific absorption rate
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Magnetic-mediated hyperthermia for cancer treatment:Research progress and clinical trials 被引量:2
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作者 赵凌云 刘嘉毅 +4 位作者 欧阳伟炜 李丹叶 李立 李利亚 唐劲天 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第10期32-45,共14页
Research progress and frontiers of magnetic-mediated hyperthermia (MMH) are presented, along with clinical trials in Germany, the US, Japan, and China. Special attention is focused on MMH mediated by magnetic nanopa... Research progress and frontiers of magnetic-mediated hyperthermia (MMH) are presented, along with clinical trials in Germany, the US, Japan, and China. Special attention is focused on MMH mediated by magnetic nanoparticles, and multifunctional magnetic devices for cancer multimodality treatment are also introduced. 展开更多
关键词 aagnetic-mediated hyperthermia magnetic nanoparticles alternative magnetic field magneticLanoparticle hyperthermia THERMOCHEMOTHERAPY
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Bioelectromagnetic Paradigm of Cancer Treatment—Modulated Electro-Hyperthermia (mEHT) 被引量:3
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作者 Oliver Szasz 《Open Journal of Biophysics》 2019年第2期98-109,共12页
One of the most frequently applied bioelectromagnetic effects is the deep heating of the living species with EMF energy. Despite its long history, hyperthermia is a rarely applied oncotherapy. The reason is its contro... One of the most frequently applied bioelectromagnetic effects is the deep heating of the living species with EMF energy. Despite its long history, hyperthermia is a rarely applied oncotherapy. The reason is its controversial results and complicated control. One of the solutions is concentrating the electromagnetic energy nanoscopically on the parts of the malignant cells instead of heating up the complete tumor-mass. This approach is a kind of non-uniform energy absorption, providing energy liberation only in the selected regions. The energy-absorption of the malignant cells targets the membranes and creates a situation far from thermal equilibrium. The selection of the malignant cells is based on their decided differences from their healthy counterparts. The distinguishing parameters are the electromagnetic properties of the components of the malignant tissue which are the physiologic differences between the malignant cells and their healthy counterparts. The targets realize nano-range heating, using natural nanoclusters on the cell-membrane without artificially implementing them. This energy absorption generates consequent reactions, like programmed cell-death (apoptosis) continued by immunogenic cell-death involving extended immune reactions.? The applied radiofrequency current is amplitude modulated by time-fractal modulation pattern. The accurately matched impedance realizes the self-selective mechanisms which are promoted by stochastic resonances. This complex method is a new kind of hyperthermia, named mEHT. Our objective is to analyze the problems of the selective, non-equilibrium energy absorption, and present a solution by the electromagnetic mechanisms for an effective and controllable hyperthermia in oncology. 展开更多
关键词 Electromagnetic Effects RF-Current Warburg-Effect Szent-Gyorgyi-Effect Fractal-Physiology Oncology hyperthermia Nanoscale Heating MODULATED Electro-hyperthermia Nanothermia mEHT Apoptosis Immunogenic Cell-Death
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Intraperitoneal perfusion of cytokine-induced killer cells with local hyperthermia for advanced hepatocellular carcinoma 被引量:21
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作者 Xiao-Pu Wang Meng Xu +2 位作者 Hong-Fei Gao Jian-Fu Zhao Ke-Cheng Xu 《World Journal of Gastroenterology》 SCIE CAS 2013年第19期2956-2962,共7页
AIM:To study the effect and tolerance of intraperitoneal perfusion of cytokine-induced killer(CIK) cells in combination with local radio frequency(RF) hyperthermia in patients with advanced primary hepatocellular carc... AIM:To study the effect and tolerance of intraperitoneal perfusion of cytokine-induced killer(CIK) cells in combination with local radio frequency(RF) hyperthermia in patients with advanced primary hepatocellular carcinoma(HCC).METHODS:Patients with advanced primary HCC were included in this study.CIK cells were perfused intraperitoneal twice a week,using 3.2 × 10 9 to 3.6 × 10 9 cells each session.Local RF hyperthermia was performed 2 h after intraperitoneal perfusion.Following an interval of one month,the next course of treatment was administered.Patients received treatment until disease progression.Tumor size,immune indices(CD3 +,CD4 +,CD3 + CD8 +,CD3 + CD56 +),alpha-fetoprotein(AFP) level,abdominal circumference and adverse events were recorded.Time to progression and overall survival(OS) were calculated.RESULTS:From June 2010 to July 2011,31 patients diagnosed with advanced primary HCC received intraperitoneal perfusion of CIK cells in combination with local RF hyperthermia in our study.Patients received an average of 4.2 ± 0.6 treatment courses(range,1-8 courses).Patients were followed up for 8.3 ± 0.7 mo(range,2-12 mo).Following combination treatment,CD4 +,CD3 + CD8 + and CD3 + CD56 + cells increased from 35.78% ± 3.51%,24.61% ± 4.19% and 5.94% ± 0.87% to 45.83% ± 2.48%(P = 0.016),39.67% ± 3.38%(P = 0.008) and 10.72% ± 0.67%(P = 0.001),respectively.AFP decreased from 167.67 ± 22.44 to 99.89 ± 22.05 ng/mL(P = 0.001) and abdominal circumference decreased from 97.50 ± 3.45 cm to 87.17 ± 4.40 cm(P = 0.002).The disease control rate was 67.7%.The most common adverse events were low fever and slight abdominal erubescence,which resolved without treatment.The median time to progression was 6.1 mo.The 3-,6-and 9-mo and 1-year survival rates were 93.5%,77.4%,41.9% and 17.4%,respectively.The median OS was 8.5 mo.CONCLUSION:Intraperitoneal perfusion of CIK cells in combination with local RF hyperthermia is safe,can efficiently improve immunological status,and may prolong survival in HCC patients. 展开更多
关键词 Cytokine-induced KILLER cell Radio frequency hyperthermia Primary HEPATOCELLULAR carcinoma INTRAPERITONEAL PERFUSION Clinical observation
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Synergistic Effect of Hyperthermia and Neferine on Reverse Multidrug Resistance in Adriamycin-resistant SGC7901/ADM Gastric Cancer Cells 被引量:10
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作者 黄程辉 李亚萍 +2 位作者 曹培国 谢兆霞 秦志强 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2011年第4期488-496,共9页
Multidrug resistance(MDR) plays a major obstacle to successful gastric cancer chemotherapy.The purpose of this study was to investigate the MDR reversal effect and mechanisms of hyperthermia in combination with nefe... Multidrug resistance(MDR) plays a major obstacle to successful gastric cancer chemotherapy.The purpose of this study was to investigate the MDR reversal effect and mechanisms of hyperthermia in combination with neferine(Nef) in adriamycin(ADM) resistant human SGC7901/ADM gastric cancer cells.The MDR cells were heated at 42℃ and 45℃ for 30 min alone or combined with 10 μg/mL Nef.The cytotoxic effect of ADM was evaluated by MTT assay.Cellular plasma membrane lipid fluidity was detected by fluorescence polarization technique.Intracellular accumulation of ADM was monitored with high performance liquid chromatography.Mdr-1 mRNA,P-glycoprotein(P-gp),γH2AX expression and γH2AX foci formation were determined by real-time PCR,Western blot and immunocytochemical staining respectively.It was found that different heating methods induced different cytotoxic effects.Water submerged hyperthermia had the strongest cytotoxicity of ADM and Nef combined with hyperthermia had a synergistic cytotoxicity of ADM in the MDR cells.The water submerged hyperthermia increased the cell membrane fluidity.Both water submerged hyperthermia and Nef increased the intracellular accumulation of ADM.The water submerged hyperthermia and Nef down-regulated the expression of mdr-1 mRNA and P-gp.The water submerged hyperthermia could damage DNA and increase the γH2AX expression of SGC7901/ADM cells.The higher temperature was,the worse effect was.Our results show that combined treatment of hyperthermia with Nef can synergistically reverse MDR in human SGC7901/ADM gastric cancer cells. 展开更多
关键词 gastric cancer multidrug resistance hyperthermia NEFERINE MDR-1 P-glycoprotein ADRIAMYCIN
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Nanosized As_2O_3/Fe_2O_3 complexes combined with magnetic fluid hyperthermia selectively target liver cancer cells 被引量:21
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作者 Zi-Yu Wang Jian Song Dong-Sheng Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第24期2995-3002,共8页
AIM:To study the methods of preparing the magnetic nano-microspheres of Fe2O3 and As2O3/Fe2O3 complexes and their therapeutic effects with magnetic fluid hyperthermia(MFH). METHODS:Nanospheres were prepared by chemica... AIM:To study the methods of preparing the magnetic nano-microspheres of Fe2O3 and As2O3/Fe2O3 complexes and their therapeutic effects with magnetic fluid hyperthermia(MFH). METHODS:Nanospheres were prepared by chemical co-precipitation and their shape and diameter were observed.Hemolysis,micronucleus,cell viability,and LD50 along with other in vivo tests were performed to evaluate the Fe2O3 microsphere biocompatibility.The inhibition ratio of tumors after Fe2O3 and As2O3/Fe2O3 injections combined with induced hyperthermia in xenograft human hepatocarcinoma was calculated. RESULTS:Fe2O3 and As2O3/Fe2O3 particles were round with an average diameter of 20 nm and 100 nm as observed under transmission electron microscope.Upon exposure to an alternating magnetic field(AMF),the temperature of the suspension of magnetic particles increased to 41-51℃,depending on different particle concentrations,and remained stable thereafter.Nanosized Fe2O3 microspheres are a new kind of biomaterial without cytotoxic effects.The LD50 of both Fe2O3 and As2O3/Fe2O3 in mice was higher than 5 g/kg.One to four weeks after Fe2O3 and As2O3/Fe2O3 complex injections into healthy pig livers,no significant differences were found in serum AST,ALT,BUN and Cr levels among thepigs of all groups(P>0.05),and no obvious pathological alterations were observed.After exposure to alternating magnetic fields,the inhibition ratio of the tumors was significantly different from controls in the Fe2O3 and As2O3/Fe2O3 groups(68.74% and 82.79%,respectively; P<0.01).Tumors of mice in treatment groups showed obvious necrosis,while normal tissues adjoining the tumor and internal organs did not. CONCLUSION:Fe2O3 and As2O3/Fe2O3 complexes exerted radiofrequency-induced hyperthermia and drug toxicity on tumors without any liver or kidney damage. Therefore,nanospheres are ideal carriers for tumortargeted therapy. 展开更多
关键词 Liver cancer Magnetic fluid hyperthermia NANOPARTICLE AS2O3
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Narrow line between benefit and harm: Additivity of hyperthermia to cisplatin cytotoxicity in different gastrointestinal cancer cells 被引量:6
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作者 Vaidotas Cesna Arturas Sukovas +5 位作者 Aldona Jasukaitiene Rima Naginiene Giedrius Barauskas Zilvinas Dambrauskas Saulius Paskauskas Antanas Gulbinas 《World Journal of Gastroenterology》 SCIE CAS 2018年第10期1072-1083,共12页
AIM To investigate the response to hyperthermia and chemotherapy, analyzing apoptosis, cytotoxicity, and cisplatin concentration in different digestive system cancer cells.METHODS AGS(gastric cancer cell line), Caco-2... AIM To investigate the response to hyperthermia and chemotherapy, analyzing apoptosis, cytotoxicity, and cisplatin concentration in different digestive system cancer cells.METHODS AGS(gastric cancer cell line), Caco-2(colon cancer cell line) and T3M4(pancreatic cancer cell line) were treated by cisplatin and different temperature setting(37 ℃ to 45 ℃) either in isolation, or in combination. Treatment lasted for one hour. 48 h after the treatment viability was evaluated by MTT, cell apoptosis by Annexin V-PE and 7 ADD flow cytometry. Intracellular cisplatin concentration was measured immediately after the treatment, using mass spectrometry. Isobologram analysis was performed to evaluate the mathematical combined effect of temperature and cisplatin.RESULTS AGS cells were the most sensitive to isolated application of hyperthermia. Hyperthermia, in addition to cisplatin treatment, did not provoke a synergistic effect at intervals from 37 ℃ to 41 ℃ in neither cancer cell line. However, a temperature of 43 ℃ enhanced cisplatin cytotoxicity for Caco-2 cells. Moreover, isobologram analysis revealed mathematical antagonistic effects of cisplatin and temperature combined treatment in AGS cells; variations between synergistic, additive, and antagonistic effects in Caco-2 cells; and additive and antagonistic effects in T3 M4 cells. Combined treatment enhanced initiation of cell apoptosis in AGS, Caco-2, and T3 M4 cells by 61%, 20%, and 19% respectively. The increase of intracellular cisplatin concentration was observed at 43 ℃ by 30%, 20%, and 18% in AGS, Caco-2, and T3 M4 cells, respectively.CONCLUSION In addition to cisplatin, hyperthermia up to 43 ℃ does not affect the viability of cancer cells in a synergistic manner. 展开更多
关键词 hyperthermal INTRAPERITONEAL chemotherapy CISPLATIN hyperthermia Isobolograms Gastric CANCER Pancreatic CANCER Colon CANCER
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Change in expression of apoptosis genes after hyperthermia,chemotherapy and radiotherapy in human colon cancer transplanted into nude mice 被引量:14
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作者 Han Liang Hong-Jie Zhan Bao-Gui Wang Yuan Pan Xi-Shan Hao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第32期4365-4371,共7页
AIM:To investigate the change in expression of p53 ,Bcl-2 ,and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia,chemotherapy,radiotherapy,thermochemotherapy,thermoradiotherapy and t... AIM:To investigate the change in expression of p53 ,Bcl-2 ,and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia,chemotherapy,radiotherapy,thermochemotherapy,thermoradiotherapy and thermochemoradiotherapy. METHODS:Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43℃,60 min),the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer. The mice were divided into 6 groups according to the treatment approaches:hyperthermia,chemotherapy,radiotherapy,thermochemotherapy,thermoradiotherapy,and thermochemoradiotherapy. The mice were sacrificed at different time points and the tumor tissue was taken for further procedures. The morphologic changes in membrane,cytoplasm and nuclei of tumor cells of p53,Bcl-2,and Bax after treatment,were observed by immunohistochemistry staining. RESULTS:All of the six treatment modalities down-regulated the expression of p53,Bcl-2 and up-regulated the expression of Bax at different levels. The combined therapy of hyperthermia,with chemotherapy,and/or irradiation showed a greater effect on down-regulating the expression of p53 (0.208 ± 0.009 vs 0.155 ± 0.0115,P < 0.01) and Bcl-2 (0.086 ± 0.010 vs 0.026 ± 0.0170,P < 0.01) and up-regulating Bax expression (0.091 ± 0.0013 vs 0.207 ± 0.027,P < 0.01) compared with any single therapy.CONCLUSION:Hyperthermia enhances the effect of radio-and chemotherapy on tumors by changing the expression of apoptosis genes,such as p53,Bcl-2 and Bax. 展开更多
关键词 hyperthermia Apoptosis p53 Bcl-2 Bax Nude mice Colon cancer Cell line
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Treatment of malignant glioma using hyperthermia 被引量:5
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作者 Jiahang Sun Mian Guo +3 位作者 Hengyuan Pang Jingtao Qi Jinwei Zhang Yunlong Ge 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第29期2775-2782,共8页
Thirty pathologically diagnosed patients with grade Ⅲ-Ⅳ primary or recurrent malignant glioma (tumor diameter 3-7 cm) were randomly divided into two groups. The control group underwent conventional radiotherapy an... Thirty pathologically diagnosed patients with grade Ⅲ-Ⅳ primary or recurrent malignant glioma (tumor diameter 3-7 cm) were randomly divided into two groups. The control group underwent conventional radiotherapy and chemotherapy. In the hyperthermia group, primary cases received hyperthermia treatment, and patients with recurrent tumors were treated with hyperthermia in com- bination with radiotherapy and chemotherapy. Hyperthermia treatment was administered using a 13.56-MHz radio frequency hyperthermia device. Electrodes were inserted into the tumor with the aid of a CT-guided stereotactic apparatus and heat was applied for 1 hour. Dudng 3 months a^er hyperthermia, patients were evaluated with head CT or MRI every month. Gliomas in the hyper- thermia group exhibited growth retardation or growth termination. Necrosis was evident in 80% of the heated tumor tissue and there was a decrease in tumor diameter. Our findings indicate that ra- dio frequency hyperthermia has a beneficial effect in the treatment of malignant glioma. 展开更多
关键词 neural regeneration GLIOMA radio frequency hyperthermia NECROSIS malignant tumor RECURRENCE CT MRI intracranial hypertension clinical effects grants-supported paper NEUROREGENERATION
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Clinical antiangiogenic effect of recombinant adenovirus-p53 combined with hyperthermia for advanced cancer 被引量:6
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作者 Xiaofan Li Shaowen Xiao +1 位作者 Yongheng Li Shanwen Zhang 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2013年第6期749-755,共7页
Objective: To assess the safety and clinical antiangiogenic effect of recombinant adenovirus-p53 (rAd-p53) combined with hyperthermia plus or not plus radiotherapy in advanced cancer. Methods: Expression of Vascul... Objective: To assess the safety and clinical antiangiogenic effect of recombinant adenovirus-p53 (rAd-p53) combined with hyperthermia plus or not plus radiotherapy in advanced cancer. Methods: Expression of Vascular epithelial growth factor (VEGF) after intratumoral injection of rAd-p53 was assayed by immunohistochemistry (IHC) imaging. Forty-four patients with advanced cancer were enrolled into this clinical study. The patients were intratumorally injected with rAd-p53 (Gendicine) at a dose of 1×1012 vp once a week, with a total of 4-54 (mean 7.7) times. Total of 4-29 (mean 8.5) times of hyperthermia was given to the patients. Among the 44 patients, 30 patients were concurrently added with radiotherapy of a total dose 30-76 Gy/15-38 f/3-8 w (mean 58 Gy). Results: Before and after intratumoral injection of rAd-p53, the VEGF IHC positive cell scores were 2.80 and 1.50, respectively (P=0.031). The treatment of rAd-p53 combined with hyperthermia plus or not plus radiotherapy in advanced cancer achieved CR rate of 13.60% (6/44), and PR rate of 29.6% (13/44), and thus the effective rate was 43.2%. In addition to 6 patients with CR, 19 patients (19/38, 50.0%) had low density area (LDA) of more than 50% area on CT image within tumor indicating tumor tissue necrosis. Conclusions: Our data indicate that rAd-p53 inhibits VEGF expression and angiogenesis, and promotes tumor necrosis and shrinkage induced by hyperthermia plus or not plus radiotherapy in advanced cancer. 展开更多
关键词 Vascular epithelial growth factor (VEGF) recombinant adenovirus-p53 (rAd-p53) advanced cancer hyperthermia radiotherapy
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Inductive heat property of Fe_3O_4 nanoparticles in AC magnetic field for local hyperthermia 被引量:5
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作者 ZHAO Donglin ZENG Xianwei XIA Qisheng TANG Jintian 《Rare Metals》 SCIE EI CAS CSCD 2006年第z1期621-625,共5页
Magnetite (Fe3O4) nanoparticles with different magnetic properties were prepared by coprecipitation of Fe3+ and Fe2+ with aqueous NaOH solution. The inductive heat properties of Fe3O4 nanoparticles in an alternating c... Magnetite (Fe3O4) nanoparticles with different magnetic properties were prepared by coprecipitation of Fe3+ and Fe2+ with aqueous NaOH solution. The inductive heat properties of Fe3O4 nanoparticles in an alternating current (AC) magnetic field were investigated for local hyperthermia. The maximum saturation magnetization Ms of Fe3O4 nanoparticles is 65.53 emu·g-1 under the optimum conditions of Fe3+: Fe2+ molar ratio at 1.8:1. The Ms of Fe3O4 nanoparticles decreased as the Fe3+/Fe2+ molar ratio increased. But the coercivity Hc increases with the increasing of Fe3+/Fe2+ molar ratio. Exposed in the AC magnetic field for 29 min, the temperatures of physiological saline suspension containing Fe3O4 nanoparticles were 42-97.5 ℃. The inductive heat property of Fe3O4 nanoparticles in AC magnetic field decreases as Hc increases, but increases with the increasing of Ms. The Fe3O4 nanoparticles would be useful as good thermoseeds for localized hyperthermia treatment of cancers. 展开更多
关键词 magnetite nanoparticles local hyperthermia cancer treatment biomedical applications
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Preparation and characterization of ferromagnetic fluids for hyperthermia of tumor
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作者 周彦菲 范田园 +2 位作者 杨仁杰 孙宏亮 夏启胜 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第3期187-191,共5页
Aim To prepare and characterize ferromagnetic fluids for hyperthermia of tumor. Methods Ferromagnetic fluids (FFs) of magnetite (Fe3O4) was prepared in the presence of polyethylene glycol (PEG-6000) by chemical ... Aim To prepare and characterize ferromagnetic fluids for hyperthermia of tumor. Methods Ferromagnetic fluids (FFs) of magnetite (Fe3O4) was prepared in the presence of polyethylene glycol (PEG-6000) by chemical precipitation method. The iron content of the FFs was determined by spectrophotometric method using o-phenanthroline. The FFs/PEG-6000 was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), infrared spectrometry (IR), and vibrating sample magnetometer (VSM). Heating effects of the FFs was measured in an alternating magnetic field in vitro. The hyperthermia of FFs in a rabbit was performed. Results The FFs/PEG-6000 was proved to be composed of Fe3O4 by XRD and IR. TEM showed that the ferromagnetic particles appeared to be almost spherical and dispersed well The average particle size was 13.3 ± 3.8 nm by XRD. The saturation magnetization and residual magnetization of the FFs were 23.39 A/m (1.556 emu/g) and 0.56 A/m (0.02604 emu/g), respectively. The coercive force was 12 Oe. The specific absorption rate (SAR) of FFs was 69 ± 10W/g [Fe]. After direct injection of FFs to hepatic VX2 carcinoma of a rabbit, the temperature in the core of the tumor was between 41 - 46 ℃ in an alternating magnetic field. Conclusion FFs/PEG-6000 was expected to be useful in hyperthermia of tumor. 展开更多
关键词 Ferromagnetic fluids FE3O4 Polyethylene glycol (PEG) Heating effects hyperthermia
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Hyperthermia inhibits hypoxia-induced epithelial-mesenchymal transition in HepG2 hepatocellular carcinoma cells 被引量:5
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作者 Guang-Jin Yuan Qian-Wen Li +3 位作者 Shun-Lin Shan Wu-Ming Wang Sen Jiang Xi-Ming Xu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第34期4781-4786,共6页
AIM:To investigate the effect of hyperthermia on hy-poxia-induced epithelial-mesenchymal transition (EMT) in HepG2 hepatocellular carcinoma (HCC) cells, and its mechanism. METHODS:Cells were treated with hyperthermia ... AIM:To investigate the effect of hyperthermia on hy-poxia-induced epithelial-mesenchymal transition (EMT) in HepG2 hepatocellular carcinoma (HCC) cells, and its mechanism. METHODS:Cells were treated with hyperthermia at 43 ℃ for 0.5 h, followed by incubation under hypoxic or normoxic conditions for 72 h. Cell morphology was observed. Expressions of E-cadherin and vimentin were determined by immunofluorescence assay or Western blot. The protein and mRNA expressions of Snail were also determined by Western blot and reverse transcrip-tion-polymerase chain reaction. Cell migratory capacity was evaluated. RESULTS:Hypoxia induced EMT in HepG2 cells, which was evidenced by morphological, molecular and func-tional changes, including the formation of a spindle shape and the loss of cell contact. The expression of E-cadherin was decreased but the expression of vimentin was increased; also, the migratory capability was increased by 2.2 ± 0.20-fold as compared with normoxia. However, those effects were inhibited by hyperthermia pretreatment. Furthermore, protein synthesis and mRNA expression of Snail in the cells were enhanced by hy-poxia as compared with normoxia, and also significantly inhibited by hyperthermia pretreatment. CONCLUSION:Hyperthermia may inhibit hypoxia-induced EMT in HepG2 HCC cells, and the mechanism may involve inhibition of induced expression of Snail. 展开更多
关键词 hyperthermia HYPOXIA Epithelial-mesen-chymal transition Hepatocellular carcinoma SNAIL
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Flow of a biomagnetic viscoelastic fluid:application to estimation of blood flow in arteries during electromagnetic hyperthermia,a therapeutic procedure for cancer treatment 被引量:3
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作者 J.C.MISRA A.SINHA G.C.SHIT 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2010年第11期1405-1420,共16页
The paper deals with the theoretical investigation of a fundamental problem of biomaguetic fluid flow through a porous medium subject to a magnetic field by using the principles of biomagnetic fluid dynamics (BFD). ... The paper deals with the theoretical investigation of a fundamental problem of biomaguetic fluid flow through a porous medium subject to a magnetic field by using the principles of biomagnetic fluid dynamics (BFD). The study pertains to a situation where magnetization of the fluid varies with temperature. The fluid is considered to be non-Newtonian, whose flow is governed by the equation of a second-grade viscoelastic fluid. The walls of the channel are assumed to be stretchable, where the surface velocity is proportional to the longitudinal distance from the origin of coordinates. The problem is first reduced to solving a system of coupled nonlinear differential equations involving seven parameters. Considering blood as a biomagnetic fluid and using the present analysis, an attempt is made to compute some parameters of the blood flow by developing a suitable numerical method and by devising an appropriate finite difference scheme. The computational results are presented in graphical form, and thereby some theoretical predictions are made with respect to the hemodynamical flow of the blood in a hyperthermal state under the action of a magnetic field. The results clearly indicate that the presence of a magnetic dipole bears the potential so as to affect the characteristics of the blood flow in arteries to a significant extent during the therapeutic procedure of electromagnetic hyperthermia. The study will attract the attention of clinicians, to whom the results would be useful in the treatment of cancer patients by the method of electromagnetic hyperthermia. 展开更多
关键词 biomagnetic fluid blood stretching walls porous medium electromagnetic hyperthermia
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Inhibitory Effects of Mild Hyperthermia plus Docetaxel Therapy on ER(+/–) Breast Cancer Cells and Action Mechanisms 被引量:4
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作者 吕峰 于洋 +3 位作者 张斌 梁栋 李兆明 尤伟 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第6期870-876,共7页
Summary: The purpose of this study was to verify that a combination of mild hyperthermia and do- cetaxel chemotherapy produces synergistic antitumor effects and to explore the action mechanisms of this treatment appr... Summary: The purpose of this study was to verify that a combination of mild hyperthermia and do- cetaxel chemotherapy produces synergistic antitumor effects and to explore the action mechanisms of this treatment approach. The effects of docetaxel on the proliferation of cells from the estrogen receptor (ER)-positive human breast cancer cell line MCF-7 and the ER-negative human breast cancer cell line MDA-MB-453 were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and effective experimental concentrations of docetaxel were determined. The effects of mild hy- perthermia plus docetaxel therapy on apoptosis rate in the MCF-7 and MDA-MB-453 human breast cancer cell lines were analyzed by using flow cytometry with Annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. The effects of these combined treatments on cell cycle progres- sion in the MCF-7 and MDA-MB-453 human breast cancer cell lines were examined by using flow cy- tometry. The effects of these combined treatments on the expression of apoptosis-related proteins and proteins in the mitogen-activated protein kinase (MAPK) pathways were analyzed by using Western blotting. The effects of these combined treatments on the expression of the heat shock protein 70 (HSP70) and the multi-drug resistance (MDR) gene product P-glycoprotein (Pgp) were examined by using Western blotting. The results showed that the half-maximal inhibitory concentration (IC50) of do- cetaxel for MCF-7 and MDA-MB-453 cells was 19.57±1.12 and 21.64±2.31 gmol/L respectively. Mild hyperthermia with docetaxel therapy could increase apoptosis rate in the MCF-7 and MDA-MB-453 cells. Apoptosis rate in MCF-7 and MDA-MB-453 cells was increased from (23.66±3.59)% and (18.51±3.17)% in docetaxel treatment group to (47.12±6.73)% and (55.16±7.42)% in mild hyperthermia plus docetaxel group, indicating that the mild hyperthermia and docetaxel therapeutic approaches exhib- ited significant synergistic antitumor effects. Treatments of mild hyperthermia plus docetaxel induced G2/M cell cycle arrest in the MCF-7 and MDA-MB-453 cells. Western blotting demonstrated that pro- teins in the MAPK pathway were expressed at higher levels in docetaxel-treated cells following mild hypothermia than those in cells treated with docetaxel alone. As compared with blank control group, cells from the mild hyperthermia plus docetaxel group exhibited significantly decreased B-cell lym- phoma 2 (Bcl-2) protein expression but slightly increased Bcl-2-associated X protein (Bax) expression. Western blotting results revealed that HSP70 and Pgp expression levels were significantly increased following mild hypothermia. It was concluded that treatments of mild hyperthermia plus docetaxel in- hibited the proliferation of human breast cancer cells, promoted apoptosis of breast cancer cells, and produced synergistic antitumor effects. 展开更多
关键词 mild hyperthermia DOCETAXEL human breast cancer cell lines estrogen receptor mito- gen-activated protein kinase apoptosis
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Expression change of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and Hpopolysaccharide 被引量:2
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作者 Xiao-Jun Zhang Ji Yue Ting-Bao Zhao 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2013年第4期300-304,共5页
Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly di... Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly divided into room temperature+physiological saline group (Croup C),hyperthermia+physiological saline group(Croup II),room temperature+LPS group(Croup L) and hyperthermia+LPS group(Croup HL).The rats were put in simulated climate cabin.Croup HL and Croup H were exposed in the environment at a dry bulb temperature(TDB) of(35.0±0.5)℃,while Croup L and Croup C were exposed in the environment at a TDB of(26.0±0.5)℃. The rats in Croup HL and Croup L were given tail intravenous injection of LPS 10 mg/kg,while the rats in Croup H and Croup C were given tail intravenous injection of 9 g/L NaCl 10 ml/kg. After the stress,immunohistochemical SABC staining method was used to detect the expression characteristics of TNF-αin myocardium and hepatic tissue of rats,and those rats were given routine pathological examinations.Results:The expression of TNF-αin myocardium and hepatic tissue in Croup HL was enhanced remarkably,and the tissue damages of Croup HL were severest.Conclusions:The eardiotoxieity and hepatotoxicity caused by compound stress of hyperthermia and LPS is closely related to the expression of TNF-α. 展开更多
关键词 hyperthermia LIPOPOLYSACCHARIDE SYSTEMIC inflammatory response Syndrome IMMUNOHISTOCHEMISTRY TNF-Α
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Heating Preciosity—Trends in Modern Oncological Hyperthermia 被引量:6
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作者 Oliver Szasz Marcell A. Szasz +1 位作者 Carrie Minnaar Andras Szasz 《Open Journal of Biophysics》 2017年第3期116-144,共29页
The use of hyperthermia as a treatment in oncology is a common topic for debate. Some researchers expect a breakthrough in oncological treatments with hyperthermia, whereas others have disregarded the method. Serious ... The use of hyperthermia as a treatment in oncology is a common topic for debate. Some researchers expect a breakthrough in oncological treatments with hyperthermia, whereas others have disregarded the method. Serious questions concerning hyperthermia have arisen. Should homogeneous (isothermal) or heterogeneous (selective) heating being used? When we use selective heating (heterogeneity), should the entire tumour be targeted or should the malignant cells be individually selected? Does the mechanism involve thermal cell death or thermally-assisted cell death? Is the goal necrosis or apoptosis? Is hyperthermia safe as a monotherapy or does it have to be combined with conventional treatments? When the selection is local, how do we act on disseminated cells that represent a high risk of life threatening metastases? When local heating is the focus, how should it be carried out with measured and controlled? Our objective is to show how precise, selective heat transfer is necessary to remove malignant cells and, consequently, how hyperthermia as part of the immune-oncology can change the game in this promising field of oncological therapies. 展开更多
关键词 HEATING Preciosity hyperthermia ONCOLOGY IMMUNE Effects Bystander-Effect Abscopal EFFECT
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