期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells 被引量:1
1
作者 HONG Wen Xu YE Jin Bo +10 位作者 CHEN Mou Tong YAN Yan ZHOU Gui Feng YANG Xi Fei YANG Liang REN Xiao Hu HUANG Hai Yan ZHOU Li HUANG Xin Feng ZHUANG Zhi Xiong LIU Jian Jun 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第7期618-621,共4页
Trichloroethylene (TCE) is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TC... Trichloroethylene (TCE) is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells. However, the molecular mechanisms by which TCE induces hepatotoxicity are not well understood. SET (also known as protein phosphatase 2A inhibitor, 12PP2A, or template-activating factor-I, TAF-D is a nuclear protein that regulates histone modification, gene transcription, DNA replication, nucleosome assembly, 展开更多
关键词 SET As TCE Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the expression of SET-Associated Proteins in Human hepatic L-02 Cells
下载PDF
Protein expression and antigenicity detection of hepatitis C virus envelope protein E2
2
《中国输血杂志》 CAS CSCD 2001年第S1期331-,共1页
关键词 Protein expression and antigenicity detection of hepatitis C virus envelope protein E2
下载PDF
Expression and semi-quantification of hepatitis B virus reverse transcriptase protein in a prokaryotic system
3
作者 CHAO ZHAO YONG XIANG WANG ZHENG HONG YUAN YU MEI WEN 《Journal of Microbiology and Immunology》 2006年第3期189-193,共5页
The reverse transcriptase (RT) protein of hepatitis B virus (HBV) has been successfully expressed by recombinant technology in Eschericahia coli ( E. coli ). In this study we aimed to develop a semi-quantitative... The reverse transcriptase (RT) protein of hepatitis B virus (HBV) has been successfully expressed by recombinant technology in Eschericahia coli ( E. coli ). In this study we aimed to develop a semi-quantitative assay for the study of HBV RT protein using this system. Complete HBV polymerase gene from a wild type virus (rt306P) and the polymerase gene from a mutant, with rt306P substituted by serine (rtP306S) were separately fused to the maltose binding protein (MBP) gene and expressed in E. coli respectively. The expression levels of HBV polymerase genes from the wild type virus and its counterpart mutant at rt306 were compared. When these proteins were semi-quantified by Westem blotting using rabbit anti-TP serum, the rtP306S mutant showed decreased expression of MBP-HBV polymerase. By this method, we have shown that the expression level of HBV RT could be affected by substitutions in its amino acid sequences, and this method could be used to study the characteristics of HBV RT protein. 展开更多
关键词 Hepatitis B virus DNA polymerase expression Mutants
下载PDF
STRUCTURE IN THE PRECORE REGION OF HEPATITIS B CORE GENE AFFECTING ITS EXPRESSION IN E.coil
4
作者 马贤凯 丁广治 +4 位作者 石成华 左平 逯好英 卢秀桂 黄翠芬 《Science China Chemistry》 SCIE EI CAS 1987年第11期1190-1198,共9页
Restriction fragments of HBV-DNA, cleaved by endonuclease HhaI,containing HBcAg gene were trimmed by BAL-31 exonuclease to remove different lengths of the precore sequence.They were inserted into plasmid pUR222 at Eco... Restriction fragments of HBV-DNA, cleaved by endonuclease HhaI,containing HBcAg gene were trimmed by BAL-31 exonuclease to remove different lengths of the precore sequence.They were inserted into plasmid pUR222 at EcoRI site through synthetic linker ligation. Transformants in E.coli BMH7118 showing different levels of HBcAg gene expression were screened and analyzed for their nucleotide sequences in the junction region both by Maxam and Gilbert's chemical degradation method and by M13 chain termination method. Results of sequence analysis of different transformants revealed a partial palindromic (loop and stem) structure, at -7 to -35 nucleotide with regard to ATG of the HBcAg gene as position +1, which has dramatic effect on the level of expression of the inserted gene using the same promoter,SD sequence and identical N-terminus.The amount of HBcAg synthesized differed from 9% in the high expressing plasmid to less than 0.01% of the total cell proteins in the low expressing transformants.The findings were compared to results obtained by other workers in studies of HBcAg expression in procaryotes and their significance in the expression of eucaryotic genes in procaryotic cells were discussed. 展开更多
关键词 HBeAg HBcAg STRUCTURE IN THE PRECORE REGION OF HEPATITIS B CORE GENE AFFECTING ITS expression IN E.coil
原文传递
A microarray analysis of early activated pathways in concanavalin A-induced hepatitis 被引量:2
5
作者 Qing-yi CAO Feng CHEN Jie LI Shan-shan WU Jing WANG Zhi CHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第5期366-377,共12页
Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and progression.Methods:Twelve BALB/c mice were divided into four groups:one ... Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and progression.Methods:Twelve BALB/c mice were divided into four groups:one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline(PBS),and another three groups with concanavalin A(Con A) administration sacrificed at 1,3,and 6 h after injection.Affymetrix GeneChip Mouse 430 2.0 Array was employed to evaluate the expression profile of each of the 12 samples.Further analysis was done on the microarray data to extract the genes that were differentially expressed.Enrichment analysis was carried out to determine relevant pathways within which regulated genes were significantly enriched.Results:A total of 393,8354 and 11 344 differentially expressed genes were found,respectively,at three time points.During 0-1 h and 1-3 h,most of the pathways enriched with regulated genes were related to immune response and inflammation,among which Toll-like receptor(TLR) signaling and mitogen-activated protein kinase(MAPK) signaling appeared during both phases,while cytokine-cytokine receptor interaction,apoptosis,T cell receptor signaling,and natural killer(NK) cell-mediated cytotoxicity pathways emerged during the second phase.Pathways found to be significant during 3-6 h were mostly related to metabolic processes.Conclusion:The TLR signaling pathway dominates the early responses of Con A-induced FH in mice.It stimulates the production of type I cytokines,therefore recruiting and activating T/NK cells.Activated T/NK cells exert their cytotoxicity on hepatocytes through inducing death receptorintermediated apoptosis,resulting in liver injury. 展开更多
关键词 Concanavalin A Fulminant hepatitis Microarray expression profile Pathway analysis
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部