目的:尘螨过敏原Der p 7新的IgE抗原表位鉴定及尘螨交叉反应性的研究。方法:ELISA检测华东地区安徽省合肥市的尘螨过敏患者与尘螨过敏临床相关性较高的重组蛋白Der p 7的IgE结合率;通过ELISA和Dot Blot鉴定屋尘螨过敏原Der p 7的新IgE...目的:尘螨过敏原Der p 7新的IgE抗原表位鉴定及尘螨交叉反应性的研究。方法:ELISA检测华东地区安徽省合肥市的尘螨过敏患者与尘螨过敏临床相关性较高的重组蛋白Der p 7的IgE结合率;通过ELISA和Dot Blot鉴定屋尘螨过敏原Der p 7的新IgE抗原表位;利用圆二色光谱法检测Der p 7和其突变体的结构及热稳定性;最后用ELISA和ELISA抑制实验鉴定尘螨新的交叉过敏物质。结果:通过ELISA实验检测发现,华东地区的合肥市内尘螨过敏患者体内IgE与Der p 7的结合率为36.2%,与华南地区的广州市(37.4%)和西班牙(34.7%)相近。然而,这一结合率受到地理区域、自然环境和生活方式等多种因素的影响,与华北地区的北京市(19.3%)、乌克兰(22.67%)、意大利(28%)相比明显较低,而与非洲(56%)和日本(超过60%)相比则显著不同。ELISA和Dot Blot鉴定出尘螨重要过敏原Der p 7的两个新IgE抗原结合表位,分别是第36位天冬氨酸和第100位天冬氨酸。用圆二色光谱实验证明了Der p 7的结构稳定性,且突变后结构未发生改变,并检测了其热稳定性特征。最后用ELISA和ELISA抑制实验新发现了两种与尘螨存在交叉过敏的物质(小麦和花生)。结论:基于尘螨过敏原Der p 7与华东地区安徽省合肥市内过敏患者IgE的结合率及新的IgE抗原表位的研究为预防、诊断、治疗过敏性疾病提供理论基础,对开发尘螨过敏原低敏疫苗具有参考价值。发现新的尘螨交叉过敏物质为交叉过敏反应的预防、治疗提供了科学依据。展开更多
Gut microbiota plays an important role in food allergy.The immunoglobulin G(IgG)/immunoglobulin E(IgE)binding capacity and human gut microbiota changes of digestion products derived from glycated ovalbumin(OVA)were in...Gut microbiota plays an important role in food allergy.The immunoglobulin G(IgG)/immunoglobulin E(IgE)binding capacity and human gut microbiota changes of digestion products derived from glycated ovalbumin(OVA)were investigated.Gastrointestinal digestion effectively destroyed the primary structure of glycated OVA,resulting in a significantly higher digestibility than gastric digestion,and more abundant peptides<3 kDa.Moreover,gastric and gastrointestinal digestion products have different fluorescence quenching and red shift of fluorescence peaks,and possess different conformational structures.These changes resulted in a decrease in 28.7%of the IgE binding capacity of gastrointestinal digestion products beyond that of pepsin.Moreover,gastrointestinal digestion products of glycated OVA increased significantly the proportion of Subdoligranulum,Collinsella,and Bifidobacterium.Therefore,gastrointestinal digestion products of glycated OVA altered human intestinal microbiota,reducing the risk of potential allergy.展开更多
目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期...目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。展开更多
目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)...目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)检测。结果:吸入过敏原组1634例,食物过敏原组2086例,吸入过敏原组阳性率为38.2%,排名前3位依次为粉尘螨(40.8%)、户尘螨(39.2%)、链格孢(25.9%);食物过敏原组阳性率为49.8%,排名前3位依次为蛋白(32.2%)、牛奶(24.7%)、小麦(18.4%)。除了粉尘螨、户尘螨与链格孢,其他不同过敏原检测分级阳性样本中sIgE的浓度均以1~3级为主。除了普通豚草外,男女童组间不同种过敏原slgE阳性率均存在差异,且差异均具有统计学意义(均P Objective: Exploring the retrospective study on the distribution characteristics and patterns of allergens in children treated at Qingdao University Affiliated Hospital, in order to provide effective basis for the diagnosis and treatment of allergic diseases in children. Method: Collect 0~14-year-old children with allergic or suspected allergic diseases who visited our hospital from January 2021 to December 2022 for serum specific allergen IgE testing. Result: There were 1634 cases in the inhaled allergen group and 2086 cases in the food allergen group. The positive rate of the inhaled allergen group was 38.2%, and the top three were dust mites (40.8%), household dust mites (39.2%), and Streptomyces (25.9%);The positive rate of the food allergen group was 49.8%, with the top three ranked in sequence being protein (32.2%), milk (24.7%), and wheat (18.4%). Except for dust mites, household dust mites, and Streptomyces, the concentration of sIgE in positive samples with different allergen detection grades is mainly in grades 1~3. Except for ordinary ragweed, there were differences in the positive rates of different allergens SLgE between male and female groups, and the differences were statistically significant (all P < 0.05). The distribution characteristics of slgE allergens vary among different age groups. The positive rate of food allergens slgE in the infant group is higher than that in other age groups. The three common allergens are milk (58%), crab (56%), and protein (53%);Except for dog dander and cat dander, the positive rate of inhaled allergen SLgE in the preschool group was higher than that in the other two age groups. The three common inhaled allergens in the preschool group were household dust mites (56%), German cockroaches (53%), and dust mites (51%), and the differences between the three age groups were statistically significant (all P < 0.01). Conclusion: The distribution of allergens in children is different in terms of gender, age and intensity. By studying the distribution of serum allergens, we can provide more effective prevention and treatment for children.展开更多
文摘目的:尘螨过敏原Der p 7新的IgE抗原表位鉴定及尘螨交叉反应性的研究。方法:ELISA检测华东地区安徽省合肥市的尘螨过敏患者与尘螨过敏临床相关性较高的重组蛋白Der p 7的IgE结合率;通过ELISA和Dot Blot鉴定屋尘螨过敏原Der p 7的新IgE抗原表位;利用圆二色光谱法检测Der p 7和其突变体的结构及热稳定性;最后用ELISA和ELISA抑制实验鉴定尘螨新的交叉过敏物质。结果:通过ELISA实验检测发现,华东地区的合肥市内尘螨过敏患者体内IgE与Der p 7的结合率为36.2%,与华南地区的广州市(37.4%)和西班牙(34.7%)相近。然而,这一结合率受到地理区域、自然环境和生活方式等多种因素的影响,与华北地区的北京市(19.3%)、乌克兰(22.67%)、意大利(28%)相比明显较低,而与非洲(56%)和日本(超过60%)相比则显著不同。ELISA和Dot Blot鉴定出尘螨重要过敏原Der p 7的两个新IgE抗原结合表位,分别是第36位天冬氨酸和第100位天冬氨酸。用圆二色光谱实验证明了Der p 7的结构稳定性,且突变后结构未发生改变,并检测了其热稳定性特征。最后用ELISA和ELISA抑制实验新发现了两种与尘螨存在交叉过敏的物质(小麦和花生)。结论:基于尘螨过敏原Der p 7与华东地区安徽省合肥市内过敏患者IgE的结合率及新的IgE抗原表位的研究为预防、诊断、治疗过敏性疾病提供理论基础,对开发尘螨过敏原低敏疫苗具有参考价值。发现新的尘螨交叉过敏物质为交叉过敏反应的预防、治疗提供了科学依据。
基金sopported by National Natural Science Foundation of China(31960457)Jiangxi Province National Science and Technology Prizes Backup Project and Cultivation Plan(20212AEI91001).
文摘Gut microbiota plays an important role in food allergy.The immunoglobulin G(IgG)/immunoglobulin E(IgE)binding capacity and human gut microbiota changes of digestion products derived from glycated ovalbumin(OVA)were investigated.Gastrointestinal digestion effectively destroyed the primary structure of glycated OVA,resulting in a significantly higher digestibility than gastric digestion,and more abundant peptides<3 kDa.Moreover,gastric and gastrointestinal digestion products have different fluorescence quenching and red shift of fluorescence peaks,and possess different conformational structures.These changes resulted in a decrease in 28.7%of the IgE binding capacity of gastrointestinal digestion products beyond that of pepsin.Moreover,gastrointestinal digestion products of glycated OVA increased significantly the proportion of Subdoligranulum,Collinsella,and Bifidobacterium.Therefore,gastrointestinal digestion products of glycated OVA altered human intestinal microbiota,reducing the risk of potential allergy.
文摘目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。
文摘目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)检测。结果:吸入过敏原组1634例,食物过敏原组2086例,吸入过敏原组阳性率为38.2%,排名前3位依次为粉尘螨(40.8%)、户尘螨(39.2%)、链格孢(25.9%);食物过敏原组阳性率为49.8%,排名前3位依次为蛋白(32.2%)、牛奶(24.7%)、小麦(18.4%)。除了粉尘螨、户尘螨与链格孢,其他不同过敏原检测分级阳性样本中sIgE的浓度均以1~3级为主。除了普通豚草外,男女童组间不同种过敏原slgE阳性率均存在差异,且差异均具有统计学意义(均P Objective: Exploring the retrospective study on the distribution characteristics and patterns of allergens in children treated at Qingdao University Affiliated Hospital, in order to provide effective basis for the diagnosis and treatment of allergic diseases in children. Method: Collect 0~14-year-old children with allergic or suspected allergic diseases who visited our hospital from January 2021 to December 2022 for serum specific allergen IgE testing. Result: There were 1634 cases in the inhaled allergen group and 2086 cases in the food allergen group. The positive rate of the inhaled allergen group was 38.2%, and the top three were dust mites (40.8%), household dust mites (39.2%), and Streptomyces (25.9%);The positive rate of the food allergen group was 49.8%, with the top three ranked in sequence being protein (32.2%), milk (24.7%), and wheat (18.4%). Except for dust mites, household dust mites, and Streptomyces, the concentration of sIgE in positive samples with different allergen detection grades is mainly in grades 1~3. Except for ordinary ragweed, there were differences in the positive rates of different allergens SLgE between male and female groups, and the differences were statistically significant (all P < 0.05). The distribution characteristics of slgE allergens vary among different age groups. The positive rate of food allergens slgE in the infant group is higher than that in other age groups. The three common allergens are milk (58%), crab (56%), and protein (53%);Except for dog dander and cat dander, the positive rate of inhaled allergen SLgE in the preschool group was higher than that in the other two age groups. The three common inhaled allergens in the preschool group were household dust mites (56%), German cockroaches (53%), and dust mites (51%), and the differences between the three age groups were statistically significant (all P < 0.01). Conclusion: The distribution of allergens in children is different in terms of gender, age and intensity. By studying the distribution of serum allergens, we can provide more effective prevention and treatment for children.
