The internal region of bacterial translocatable IS1 acts as a cis-element to stimulate transcription from the various promoters located upstream. The product of the artA gene is genetically shown to stimulate transcri...The internal region of bacterial translocatable IS1 acts as a cis-element to stimulate transcription from the various promoters located upstream. The product of the artA gene is genetically shown to stimulate transcription with the cis-element. Here, a codon-optimized artA gene was synthesized and cloned to express the ArtA protein. ArtA was purified as the Histagged protein. Nitrocellulose filter binding assay showed that ArtA specifically binds to the IS1 internal region. Electrophoretic mobility shift assay also showed specific binding of ArtA to the IS1 internal region. These results imply that ArtA directly binds to the IS1 internal region and stimulates transcription.展开更多
In this paper, we continue the discussion of [12] to establish the Hermite pseudospectral method with weight ω（x） = 1. As an application, we consider the pseudospectral approximation of the reaction-diffusion equat...In this paper, we continue the discussion of [12] to establish the Hermite pseudospectral method with weight ω（x） = 1. As an application, we consider the pseudospectral approximation of the reaction-diffusion equation on the whole line, we prove the existence of the approximate attractor and give the error estimate for the approximate solution.展开更多
文摘The internal region of bacterial translocatable IS1 acts as a cis-element to stimulate transcription from the various promoters located upstream. The product of the artA gene is genetically shown to stimulate transcription with the cis-element. Here, a codon-optimized artA gene was synthesized and cloned to express the ArtA protein. ArtA was purified as the Histagged protein. Nitrocellulose filter binding assay showed that ArtA specifically binds to the IS1 internal region. Electrophoretic mobility shift assay also showed specific binding of ArtA to the IS1 internal region. These results imply that ArtA directly binds to the IS1 internal region and stimulates transcription.
基金Supported by the National Natural Sciences Foundation of China (No. 10771142) and (No. 10671130)
文摘In this paper, we continue the discussion of [12] to establish the Hermite pseudospectral method with weight ω（x） = 1. As an application, we consider the pseudospectral approximation of the reaction-diffusion equation on the whole line, we prove the existence of the approximate attractor and give the error estimate for the approximate solution.
