目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD...目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD模型,将造模成功的36只大鼠随机分为模型组、治疗组和治疗+咖啡酸组,每组12只。记录大鼠吞咽潜伏期和吞咽次数,生物信号采集器检测舌下神经放电、舌肌阈强度和收缩幅度,酶联免疫吸附测定血清P物质含量,甲苯胺蓝染色检测舌下神经核尼氏体数目,免疫组织化学检测舌下神经核TRPV1、五羟色胺(5-HT)、磷酸化p38、神经元型一氧化氮合酶(nNOS)蛋白表达水平。结果与正常组比较,模型组大鼠吞咽潜伏期、吞咽次数、舌下神经放电积分面积、舌肌收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平下降,舌肌阈强度和舌下神经核磷酸化p38、nNOS蛋白表达水平增加(P<0.05);与模型组比较,治疗组大鼠舌肌单收缩幅度、舌肌强直收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平增加[2.36±0.26 vs 1.77±0.22、3.46±0.36 vs 2.15±0.18、(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml、(33.60±3.65)个vs(24.60±2.34)个、(19.85±2.11)%vs(9.79±1.07)%、(22.43±2.34)%vs(10.85±1.13)%,P<0.05]。结论电针廉泉穴可能通过激活TRPV1信号通路改善PSD大鼠神经功能缺损。展开更多
To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods： Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China Natio...To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods： Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China National Knowledge Infrastructure （CNKI）, Wanfang Academic Journal Full-text Database （Wanfang）, Chongqing VIP Database （CQVIP）, PubMed and Science Direct between the time that databases were created and March 25th,2017. Relevant excel database was established and descriptive studies and association rules were analyzed. Results： The most frequently used point was Lianquan （CV 23） and the most frequently used meridian was the Stomach Meridian. The analysis of association rules showed that the clinical choice of acupuncture points was highly correlated, among which the combination of the highest degree of confidence and the highest degree of support was Shenmen （HT 7） and Sishencong （EX-HN 1）; Lieque （LU 7）, lianquan （CV 23） and Zhaohai （KI 6）. Conclusion： Acupuncture treatment of sleep apnea syndromes has specific selection rules of points, providing certain references for clinical and scientific research.展开更多
Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-m...Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database（CBM）, Chongqing VIP Database（CQVIP）, China National Knowledge Infrastructure（CNKI）, and Wanfang Academic Journal Full-text Database（Wanfang）. The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results： The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao（SP 6）, Shenshu（BL 23）, Guanyuan（CV 4）, Baihui（GV 20）, and Shenmen（HT 7）. The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were：（1） Sanyinjiao（SP 6）;（2） Shenshu（BL 23） and Guanyuan（CV 4）;（3） Baihui（GV 20）, Shenmen（HT 7）, Zusanli（ST 36）, Ganshu（BL 18） and Taichong（LR 3）;（4） Taixi（KI 3）, Pishu（BL 20）, Xinshu（BL 15）, Qihai（CV 6） and Neiguan（PC 6）;（5） Sishencong（EX-HN 1）, Zhongwan（CV 12）, Hegu（LI 4）, Yintang（GV 29）, Fengchi（GB 20）, Zhongji（CV 3） and Feishu（BL 13）. The three most significant acupoints were Sanyinjiao（SP 6）, Shenshu（BL 23） and Guanyuan（CV 4）. Acupoint groups based on syndrome differentiation included：（1） Hegu（LI 4）, Zhongwan（CV 12） and Sishencong（EX-HN 1）;（2） Feishu（BL 13）, Zhongji（CV 3）, Fengchi（GB 20） and Yintang（GV 29）;（3） Xinshu（BL 15）, Pishu（BL 20）, Qihai（CV 6）, Neiguan（PC 6） and Taixi（KI 3）;（4） Ganshu（BL 18）, Zusanli（ST 36）, Shenmen（HT 7）, Taichong（LR 3） and Baihui（GV 20）. The analysis of association pattern elaborated that Shenshu（BL 23） and Sanyinjiao（SP 6） won the highest support rate in the paired groups; Ganshu（BL 18）, Shenshu（BL 23） and Sanyinjiao（SP 6） had the highest support rate among the acupoint groups. Conclusion： The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.展开更多
文摘目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD模型,将造模成功的36只大鼠随机分为模型组、治疗组和治疗+咖啡酸组,每组12只。记录大鼠吞咽潜伏期和吞咽次数,生物信号采集器检测舌下神经放电、舌肌阈强度和收缩幅度,酶联免疫吸附测定血清P物质含量,甲苯胺蓝染色检测舌下神经核尼氏体数目,免疫组织化学检测舌下神经核TRPV1、五羟色胺(5-HT)、磷酸化p38、神经元型一氧化氮合酶(nNOS)蛋白表达水平。结果与正常组比较,模型组大鼠吞咽潜伏期、吞咽次数、舌下神经放电积分面积、舌肌收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平下降,舌肌阈强度和舌下神经核磷酸化p38、nNOS蛋白表达水平增加(P<0.05);与模型组比较,治疗组大鼠舌肌单收缩幅度、舌肌强直收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平增加[2.36±0.26 vs 1.77±0.22、3.46±0.36 vs 2.15±0.18、(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml、(33.60±3.65)个vs(24.60±2.34)个、(19.85±2.11)%vs(9.79±1.07)%、(22.43±2.34)%vs(10.85±1.13)%,P<0.05]。结论电针廉泉穴可能通过激活TRPV1信号通路改善PSD大鼠神经功能缺损。
基金supported by Hunan Provincial Innovation Foundation for Postgraduate,No.CX2017B427~~
文摘To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods： Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China National Knowledge Infrastructure （CNKI）, Wanfang Academic Journal Full-text Database （Wanfang）, Chongqing VIP Database （CQVIP）, PubMed and Science Direct between the time that databases were created and March 25th,2017. Relevant excel database was established and descriptive studies and association rules were analyzed. Results： The most frequently used point was Lianquan （CV 23） and the most frequently used meridian was the Stomach Meridian. The analysis of association rules showed that the clinical choice of acupuncture points was highly correlated, among which the combination of the highest degree of confidence and the highest degree of support was Shenmen （HT 7） and Sishencong （EX-HN 1）; Lieque （LU 7）, lianquan （CV 23） and Zhaohai （KI 6）. Conclusion： Acupuncture treatment of sleep apnea syndromes has specific selection rules of points, providing certain references for clinical and scientific research.
基金supported by Scientific Research Project of Hebei Provincial Administration Bureau of Traditional Chinese Medicine,No.2015162Hebei Tangshan Science and Technology Project,No.14130264B+1 种基金Cultivation Fund of North China University of Science and Technology,No.GP201512Undergraduate Innovation and Entrepreneurship Training Programs of North China University of Science and Technology,No.X2015203,No.X2016281~~
文摘Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database（CBM）, Chongqing VIP Database（CQVIP）, China National Knowledge Infrastructure（CNKI）, and Wanfang Academic Journal Full-text Database（Wanfang）. The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results： The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao（SP 6）, Shenshu（BL 23）, Guanyuan（CV 4）, Baihui（GV 20）, and Shenmen（HT 7）. The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were：（1） Sanyinjiao（SP 6）;（2） Shenshu（BL 23） and Guanyuan（CV 4）;（3） Baihui（GV 20）, Shenmen（HT 7）, Zusanli（ST 36）, Ganshu（BL 18） and Taichong（LR 3）;（4） Taixi（KI 3）, Pishu（BL 20）, Xinshu（BL 15）, Qihai（CV 6） and Neiguan（PC 6）;（5） Sishencong（EX-HN 1）, Zhongwan（CV 12）, Hegu（LI 4）, Yintang（GV 29）, Fengchi（GB 20）, Zhongji（CV 3） and Feishu（BL 13）. The three most significant acupoints were Sanyinjiao（SP 6）, Shenshu（BL 23） and Guanyuan（CV 4）. Acupoint groups based on syndrome differentiation included：（1） Hegu（LI 4）, Zhongwan（CV 12） and Sishencong（EX-HN 1）;（2） Feishu（BL 13）, Zhongji（CV 3）, Fengchi（GB 20） and Yintang（GV 29）;（3） Xinshu（BL 15）, Pishu（BL 20）, Qihai（CV 6）, Neiguan（PC 6） and Taixi（KI 3）;（4） Ganshu（BL 18）, Zusanli（ST 36）, Shenmen（HT 7）, Taichong（LR 3） and Baihui（GV 20）. The analysis of association pattern elaborated that Shenshu（BL 23） and Sanyinjiao（SP 6） won the highest support rate in the paired groups; Ganshu（BL 18）, Shenshu（BL 23） and Sanyinjiao（SP 6） had the highest support rate among the acupoint groups. Conclusion： The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.
