Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer

AIM To investigate the anticancer mechanisms of the monoterpenoid alcohol linalool in human colon cancer cells.METHODS The cytotoxic effect of linalool on the human colon cancer cell lines and a human fibroblast cell line was examined using the WST-8 assay. The apoptosisinducing effect of linalool was measured using the terminal deoxynucleotidyl transferase d UTP nickend labeling assay and flow cytometry with Annexin V. Oxidative stress was investigated by staining for diphenyl-1-pyrenylphosphine, which is a cellular lipid peroxidation marker, and electron spin resonance spectroscopy. Sixteen SCID mice xenografted with human cancer cells were randomized into 3 groups for in vivo analysis: control and low-dose and high-dose linalool groups. The control group was administered tap water orally every 3 d. The linalool treatment groups were administered 100 or 200 μg/kg linalool solution orally for the same period. All mice were sacrificed under anesthesia 21 d after tumor inoculation, and tumors and organs were collected for immunohistochemistry using an anti-4-hydroxynonenal antibody. Tumor weights were measured and compared between groups. RESULTS Linalool induced apoptosis of cancer cells in vitro, following the cancer-specific induction of oxidative stress, which was measured based on spontaneous hydroxyl radical production and delayed lipid peroxidation. Mice in the high-dose linalool group exhibited a 55% reduction in mean xenograft tumor weight compared with mice in the control group(P < 0.05). In addition, tumor-specific lipid peroxidation was observed in the in vivo model.CONCLUSION Linalool exhibited an anticancer effect via cancerspecific oxidative stress, and this agent has potential for application in colon cancer therapy.

Expression in Arabidopsis of a Strawberry Linalool Synthase Gene Under the Control of the Inducible Potato PI2 Promoter

To investigate the role of inducible linalool in Arabidopsis-insect interactions, the FANES 1 linalool synthase （LIS） cDNA from strawberry with plastid targeting and a synthetic intron （LIS＇） was placed under the control of the wound inducible proteinase inhibitor 2 （PI2） promoter from potato. The construct pBin-PPi2-LIS＇ was transformed to Arabidopsis thaliana ecotype Columbia 0. Kanamycin resistant TO seedlings were confirmed for the presence and transcription of the LIS＇ gene by PCR analysis on genomic DNA and by RT-PCR analysis on RNA. Genomic and RT-PCR products were sequenced to confirm correct splicing of the synthetic intron. The expression of active linalool synthase by the PPI2-LIS＇ gene construct in the transgenic lines was assessed by measuring linalool emission using solid phase micro-extraction （SPME） GC-MS measurements after induction with methyl jasmonate. Among 30 tested independent T2 transgenic lines, 10 exhibited linalool production. Linalool expression could be induced by methyl jasmonate treatment, but not by diamondback moth larvae.

Putative Mode of Action of the Monoterpenoids Linalool, Methyl Eugenol, Estragole, and Citronellal on Ligand-Gated Ion Channels

Essential oil has been used as sedatives,anticonvulsants,and local anesthetics in traditional medical remedies;as preservatives for food,fruit,vegetable,and grain storage;and as bio-pesticides for food production.Linalool(LL),along with a few other major components such as methyl eugenol(ME),estragole(EG),and citronellal,are the active chemicals in many essential oils such as basil oil.Basil oil and the aforementioned monoterpenoids are potent against insect pests.However,the molecular mechanism of action of these chemical constituents is not well understood.It is well-known that the c-aminobutyric acid type A receptors(GABAARs)and nicotinic acetylcholine receptor(nAChR)are primary molecular targets of the synthetic insecticides used in the market today.Furthermore,the GABAAR-targeted therapeutics have been used in clinics for many decades,including barbiturates and benzodiazepines,to name just a few.In this research,we studied the electrophysiological effects of LL,ME,EG,and citronellal on GABAAR and nAChR to further understand their versatility as therapeutic agents in traditional remedies and as insecticides.Our results revealed that LL inhibits both GABAAR and nAChR,which may explain its insecticidal activity.LL is a concentration-dependent,noncompetitive inhibitor on GABAAR,as the half-maximal effective concentration(EC50)values of c-aminobutyric acid(GABA)for the rat a1b3c2L GABAAR were not affected by LL:(36.2±7.9)lmol-1 and(36.1±23.8)μmol·L-1 in the absence and presence of 5 mmol·L-1 LL,respectively.The half-maximal inhibitory concentration(IC50)of LL on GABAAR was approximately 3.2 mmol·L-1.Considering that multiple monoterpenoids are found within the same essential oil,it is likely that LL has a synergistic effect with ME,which has been previously characterized as both a GABAAR agonist and a positive allosteric modulator,and with other monoterpenoids,which offers a possible explanation for the sedative and anticonvulsant effects and the insecticidal activities of LL.

Ferric Chloride Catalyzed Isomerization and Cyclization of Geraniol, Linalool and Nerol

Ferric chloride catalyzes the isomerization and cyclization of geraniol, linalool and nerol in acetonitrile giving a-terpineol in good to high yields.

Supercritical Equilibrium Data of the Systems Carbon Dioxide—Linalool and Carbon Dioxide—Orange Essential Oil

In this paper experimental equilibrium data on the system supercritical CO2-orange essential oil and the system supercritical CO2-linalool are reported at 323.15 K and 343.15 K, for pressures in the ranges of 7.6-13.5 MPa. The behavior of the system supercritical CO2-orange essential oil was represented by means of thermodynamic model, based on Peng-Robinson equation of state. To this aim the orange essential oil was represented by a mixture of limonene, linalool and β-caryophyllene, selected to represent the classes of monoterpenes, oxygenated terpenes and sesquiterpenes respectively. The model uses only regression parameters calculated from binary sub-systems, CO2-limonene and CO2-β-caryophyllene (taken from literature) and CO2-linalool (calculated from the fitting of original data reported in the present work) thus being predictive with respect to the multicomponent mixture.

Subchronic Administration of Linalool Decreases Depressive-Like Behaviour in Restrained Rats

Objectives： The aim of this study was to investigate the effect of linalool in chronically stressed rats on their behaviour as related to depressive disorders and BDNF （brain-derived neurotropic factor） protein in the hippocampus. Methods： Either Tween 80 or linalool （50, 160, 500 mg/kg） was intraperitonealty administered to rats, daily, for two weeks. Some rats were housed in home cages but the others were induced with chronic restrained stress （15 min daily）. At the end of the treatment, the rats were assessed for depressive-like behaviour using the forced swimming test. At the end of the behaviour test, the animals were immediately decapitated and the hippocampus of each animal was removed for the measurement of the BDNF protein by ELISA. Result： The immobility time was significantly increased （p 〈 0.05） but time of climbing was significantly decreased （p 〈 0.05）. The restrained rats treated with linalool, 500 mg/kg, displayed immobility times less than those of their controls （p 〈 0.05） while these rats showed significantly more climbing than in the control rats （p 〈 0.05）. Linalool showed no effect on the BDNF protein in the hippocampus. Conclusions： linalool decreases behaviour related to depressive disorders but it has no effect on the BDNF protein in chronic restrained stress.

阜阳地区6种杂薰衣草的引种栽培

为探讨杂薰衣草在阜阳地区的生态适应性与应用潜质,以6种杂薰衣草引种品种为研究对象,在栽植第3年对株高、冠幅、花枝数等生长指标,叶色、花色、花期等观赏指标,精油含量、成分、产量等经济指标进行调查分析。结果显示,在阜阳地区各品种均能正常生长,其中‘Mentha’株高最高,达81.30 cm;‘Sumian’冠幅最大,达114.25 cm;‘Nizza’花量最大,干重达252.63 g/株;‘Arabian Nights’花萼、花冠颜色深,花期最早,观赏期最长。经济指标检测显示,‘Arabian Nights’精油提取率最高,达5.34%;‘Nizza’精油产量最高,达9.22 g/株。气相色谱-质谱联用仪(GC-MS)分析显示,‘Nizza’精油成分中芳樟醇、乙酸芳樟酯含量较高,品质显著优于其他品种。综上,在阜阳地区,‘Arabian Nights’最适宜用于观赏栽培,‘Nizza’最适宜用于精油提取栽培。

茶树橙花叔醇和芳樟醇樱草糖苷含量全基因组关联分析及候选基因预测

橙花叔醇与芳樟醇是广泛分布于植物中的挥发性萜烯醇类化合物,在茶树新梢中主要以樱草糖苷形式存在,提高其含量对茶叶香气品质改良具有重要意义。为揭示茶树橙花叔醇和芳樟醇樱草糖苷的遗传机制,本研究以169个茶树自然杂交后代单株为关联群体,利用均匀分布在茶树染色体上的675,245个SNP标记,对3个年份下茶树新梢中橙花叔醇与芳樟醇樱草糖苷含量进行全基因组关联分析(genome-wide association study,GWAS)。结果表明,橙花叔醇与芳樟醇樱草糖苷含量的表型变异系数为60.83%~80.08%,广义遗传力分别为51.29%与61.87%,基本符合正态分布,具有典型的数量性状遗传特征。全基因组关联分析共检测到50个显著关联位点,各位点分别对橙花叔醇和芳樟醇樱草糖苷含量变化的贡献率均超过20%,其中橙花叔醇樱草糖苷含量(nerolidol primeveroside content,NPC)变化位点最大贡献率为38.73%,芳樟醇樱草糖苷含量(linalool primeveroside content,LPC)变化位点最大贡献率为39.07%。通过等位变异效应分析,鉴定出4个主效SNP位点及其优异等位变异,并发现1个可同时调控NPC和LPC的一因多效位点。结合已有的文献报道和基因的功能注释,筛选出每个显著位点置信区间内最有可能的候选基因共59个,主要涉及茶树的糖类代谢、转录调控、萜烯类生物合成等多个生物学过程,其中26个基因在适制绿茶品种和适制乌龙茶品种单芽中的表达水平存在显著差异。本研究为深入解析茶树橙花叔醇和芳樟醇樱草糖苷含量的遗传机制提供了新的信息,为加速培育高香型优异茶树新品种提供了重要的基因资源。

芳樟醇对生姜枯萎病菌的抑制作用

为明确芳樟醇对生姜枯萎病菌尖孢镰刀菌(Fusarium oxysporum)的抑菌效果与作用机制,以不同质量浓度的芳樟醇处理尖孢镰刀菌,对尖孢镰刀菌菌丝体生长、孢子萌发和细胞膜的损伤以及防治效果进行系统研究。结果表明:芳樟醇可显著抑制尖孢镰刀菌的菌丝生长,其半数有效浓度为1.183 g/L,最小抑菌浓度(minimal inhibitory concentration,MIC)为2.0 g/L,抑制效果与芳樟醇质量浓度呈剂量效应关系;以芳樟醇MIC处理尖孢镰刀菌6 h,其孢子浓度为(1.43±0.12)×10^(4)spores/mL,显著低于对照组((5.79±0.19)×10^(4)spores/mL)(P<0.05);MIC芳樟醇处理枯萎病菌尖孢镰刀菌12 h,其孢子萌发率仅为(5.83±2.16)%,显著低于对照组((95.12±3.78)%)(P<0.05)。1/2 MIC与MIC芳樟醇处理破坏了细胞膜的完整性,导致菌丝悬液中相对电导率、核酸渗漏量显著增加,菌丝中丙二醛浓度也显著增加,麦角固醇含量显著降低,减弱了尖孢镰刀菌的致病力;MIC芳樟醇处理3 d后姜块上没有病斑产生,有良好的防治效果。综上所述,芳樟醇通过抑制生姜枯萎病菌尖孢镰刀菌菌丝生长与孢子萌发、破坏细胞结构从而影响其正常的生理功能,本研究可为防治生姜枯萎病提供新思路。

右旋芳樟醇型樟树不同优株的生理生态特性和精油含量分析

为右旋芳樟醇型樟树的良种培育及开发利用提供参考,对江西省金溪县11棵右旋芳樟醇型樟树优株(122、129、250、251、265、266、280、281、282、283、294)进行扦插栽培,测定形态指标(株高、冠幅、基径、生物量)、叶表型性状(叶长、叶宽、叶形指数、叶面积、叶周长)、生理指标(叶绿素a、b含量、可溶性糖含量、可溶性蛋白含量)及精油含量,并分析指标差异和相关关系,采用主成分分析法筛选优良优株。结果表明:11棵优株在株高、基径、冠幅、地上生物量等方面存在显著差异(P<0.05);叶表型指标中,叶面积变异程度最大,251优株叶长等指标最大,283优株叶形指数最大;总叶绿素和可溶性糖含量在优株间差异显著,蛋白质含量差异不显著,其中281优株总叶绿素(5.19 mg·L^(−1))和可溶性蛋白含量(0.21 mg·g^(−1))最高,283优株的可溶性糖含量(18.38 mg·g^(−1))最高;优株间得油率差异较大,122优株得油率(2.49%)最高;叶长、叶宽等与叶面积、叶周长存在正相关关系,可溶性蛋白含量与叶精油含量呈极显著负相关关系;聚类分析将优株分为4类,主成分分析表明251优株综合得分最高,251与280优株的综合表现较好。根据主成分分析和优株品质评价结果,251与280优株可作为金溪县栽培的右旋芳樟醇型樟树优良品种。

Oxygen vacancy engineering on cerium oxide nanowires for room-temperature linalool detection in rice aging

It is a huge challenge for metal oxide semiconductor gas sensors to inspect volatile organic compounds(VOCs)at room temperature(RT).Herein,the effective utilization of cerium oxide(CeO_(2))nanowires for RT detection of VOCs was realized via regulating its surface chemical state.Oxygen vacancy engineering on CeO_(2) nanowires,synthesized via hydrothermal method,can be manipulated by annealing under various controlled atmospheres.The sample annealed under 5%H_(2)+95%Ar condition exhibited outstanding RT sensing properties,displaying a high response of 16.7 towards 20 ppm linalool,a fast response and recovery time(16 and 121 s,respectively),and a low detection of limit of 0.54 ppm.The enhanced sensing performance could be ascribed for the synergistic effects of its nanowire morphology,the large specific surface area(83.95 m^(2)/g),and the formation of extensive oxygen vacancy accompanied by an increase in Ce^(3+).Additionally,the practicability of the sensor was verified via two varieties of rice(Indica and Japonica rice)stored in various periods(1,3,5,7,15,and 30 d).The experimental results revealed that the sensor was able to distinguish Indica rice from Japonica rice.Accordingly,the as-developed sensor delivers a strategic material to develop high-performance RT electronic nose equipment for monitoring rice quality.

Fabrication linalool-functionalized hollow mesoporous silica spheres nanoparticles for efficiently enhance bactericidal activity

To develop a novel food preservation technology for efficiently enhance bactericidal activity in a long term,hollow mesoporous silica spheres(HMSS)with regular nanostructures were applied to encapsulate natural organic antimicrobial agents.The chemical structures,morphologies and thermal stabilities of linalool,HMSS and linalool-functionalized hollow mesoporous silica spheres(L-HMSS)nanoparticles were evaluated by polarimeter,field emission scanning electron microscope(FE-SEM),transmission electron microscope(TEM),fourier transform infrared(FT-IR),thermal gravimetric analyzer(TGA),nitrogen adsorption-desorption,zeta potential and small angle X-ray diffraction(SXRD).The results show that the linalool was successfully introduced into the cavities of HMSS,and the inorganic host exhibited a high loading capacity of about 1500 mg/g.In addition,after 48 h of incubatio n,the minimum bactericidal concentrations(MBC)of L-HMSS against Escherichia coli(E.coli),Salmonella enterica(S.enterica)and Staphylococcus aureus(S.aureus),Listeria monocytogenes(L.monocytogenes)were decreased to be 4(<5)mg/mL and 8(<10)mg/mL,respectively.These results revealed linaloolfunctionalized hollow mesoporous spheres could efficiently improve the bactericidal activities of the organic component.Furthermore,SEM images clearly showed that L-HMSS indeed had an extremely inhibitory effect against gram-negative(E.coli)and gram-positive(S.aureus)by breaking the structure of the cell membrane.This research is of great significance in the application of linalool in nano-delivery system as well as food industry.

芳樟醇诱导肠炎沙门氏菌进入活的不可培养状态的研究

芳樟醇可有效降低食品在生产过程中受致病菌污染而引起食源性疾病的概率。鲜有关注由芳樟醇诱导活的不可培养状态(VBNC)细菌的抗性以及其复苏研究。采用二倍稀释法测定芳樟醇对肠炎沙门氏菌的最小抑菌浓度(MIC)为0.4%(V/V),通过PMAxx-qPCR方法对比可培养菌体细胞与VBNC状态的肠炎沙门氏菌对热、柠檬酸、乙酸、乳酸、头孢曲松钠、左氧氟沙星以及过氧化氢的耐受能力差异。采用丙酮酸钠、过氧化氢酶及对数期沙门氏菌无菌上清对VBNC细菌进行复苏。结果表明,芳樟醇诱导的VBNC状态的肠炎沙门氏菌对热、酸、抗生素的耐受能力均弱于可培养状态细胞,而对过氧化氢的耐受能力显著高于可培养沙门氏菌。经过72 h处理,只有过氧化氢酶成功复苏约(5.51±0.08)lg(CFU/mL)的肠炎沙门氏菌。通过测定可培养、VBNC以及复苏菌株内活性氧差异,表明活性氧的积累在菌体VBNC状态形成中的重要作用。本研究为监控天然抑菌物质使用过程中VBNC细菌的形成及其可能产生的食品安全问题提供了研究思路和科学依据。

一标多测法同时测定艾纳香药材中6种成分的含量

目的建立一标多测法同时测定艾纳香药材中β-蒎烯、樟脑、芳樟醇、β-石竹烯、α-石竹烯和龙脑6种主要活性成分含量。方法采用气相色谱法,色谱柱为PEG-20M(30 m×0.32 mm,10μm)石英毛细管柱,升温程序:起始温度60℃,维持2 min,以5℃·min^(-1)升至140℃,维持2 min,以2℃·min^(-1)升至150℃。高纯氮气为载气(99999%),火焰离子化检测器和进样口温度均设定为240℃。以龙脑为内参物,建立其与β-蒎烯、樟脑、芳樟醇、β-石竹烯和α-石竹烯的相对校正因子,计算各待测组分的含量,并将测定结果与用内标法测定结果进行比对。结果β-蒎烯、樟脑、芳樟醇、β-石竹烯、α-石竹烯和龙脑分别在2.01~80.4μg·mL^(-1)、6.03~241.2μg·mL^(-1)、2.00~80.0μg·mL^(-1)、6.06~242.4μg·mL^(-1)、4.04~161.6μg·mL^(-1)和80.1~3204μg·mL^(-1)浓度范围内具有良好线性关系,方法平均回收率(n=9)分别为98.4%(RSD=06%)、99.1%(RSD=1.2%)、98.9%(RSD=1.2%)、99.0%(RSD=1.3%),98.8%(RSD=1.5%)和98.5%(RSD=1.1%),与常规内标法比较,一标多测法所得结果无显著性差异。结论该方法简便快速,准确可靠,可用于艾纳香药材的质量控制。

QuEChERS-GC-MS法快速检测茶叶中的芳樟醇和alpha-紫罗兰酮

【目的】为完善茶叶中香气物质的检测方法,针对芳樟醇与alpha-紫罗兰酮两种香气物质建立气相色谱-质谱联用法(Gas Chromatography-Mass Spectrometry,GC-MS)快速同时检测的分析方法,以期为茶叶中香气物质的检测提供新思路和参考。【方法】茶叶样品经乙腈提取后,用QuEChERS净化管(内含PSA 200 mg、GCB 200 mg、无水硫酸钠400 mg)净化茶叶上清液,采用GC-MS选择离子监测模式进行测定,外标法定量。【结果】芳樟醇和alpha-紫罗兰酮在5.0~2000.0μg·L^(−1)内线性良好,线性相关系数(R^(2))均大于0.999;方法的检出限为分别为0.020 mg·kg^(−1)和0.004 mg·kg^(−1),定量限分别为0.080 mg·kg^(−1)和0.010 mg·kg^(−1)。使用该方法在空白茶叶样品中分别添加3种不同加标浓度(5.0、10.0、1000.0μg·L^(−1))的混合标准溶液,测得平均回收率为88.23%~106.11%,相对标准偏差(RSD)为1.98%~3.25%(n=6)。【结论】该方法操作快速便捷,运行时间仅需16.8 min,重现性和精确度高、灵敏度好,适用于茶叶中芳樟醇、alpha-紫罗兰酮的快速检测,能够为相关机构对茶叶中香气物质的检测提供技术支持。

Zwitterionic comb-like lipid polymers encapsulating linalool for increasing the fragrance retention time

Fragrances are widely used in cosmetics,apparel and detergents.However,the rapid evaporation of the aro ma shortens the useful life of the aromatic product.Therefore,improving the fragrance retention time of aromatic products and prolonging the service life of aromatic products are the key scientific problems that need to be solved in current aromatic products.In this study,zwitterionic comb-like lipid polymers were synthesized to encapsulate the fragrance molecule linalool.The results showed that the zwitterionic comb lipid molecules were capable of encapsulating more linalool than linear lipid molecules.At the same time,the zwitterionic comb-like lipid molecules also limited the slow release rate of the aroma,thereby increasing the fragrance retention time of the nano-fragrance.

Natural variation in linalool metabolites:One genetic locus,many functions?

The ubiquitous volatile linalool is metabolized in plants to nonvolatile derivatives.We studied Nicotiana attenuata plants which naturally vary in(S)-(+)-linalool contents,and lines engineered to produce either(R)-(-)-or(S)-(+)-linalool.Only(S)-(+)-linalool production was associated with slower growth of a generalist herbivore,and a large fraction was present as nonvolatile derivatives.We found that variation in volatile linalool and its nonvolatile glycosides mapped to the same genetic locus which harbored the biosynthetic gene,NaLIS,but that free linalool varied more in environmental responses.This study reveals how(S)-(+)-linalool and conjugates differ in their regulation and possible functions in resistance.

氮素形态对樟树生长及出油效果的影响

以芳樟醇型樟树为研究对象,探讨不同硝铵比例(10∶0、7∶3、3∶7、0∶10)硝铵态氮(NO_(3)^(-)-N/NH_(4)^(+)-N)对樟树苗期及1.5年生幼树林分生长与出油效果的影响。结果表明:氮素形态对樟树苗高、地径生长影响显著。在供试4个比例氮素形态处理中,以硝铵比3∶7处理下的苗高、地径生长量最大。樟树苗期生长具有明显的节律性,其中以6-8月为生长高峰期。在硝铵比3∶7处理下,樟树幼林树高、地径、枝叶生物量最大,高径比最小,且枝叶出油率、总醇、芳樟醇含量高,而樟脑含量最低。纯硝态氮、铵态氮不利于樟树生长,两者分别显著抑制了幼林地径与树高生长量,尤其在纯硝态氮处理下,枝叶出油率及出油主成分含量最低。以检测的幼林生长及出油方面的8个指标为评价指数,通过主成分分析法对不同比例氮素形态处理进行综合评分,表现为:硝铵比3∶7>0∶10>7∶3>10∶0。研究认为,相较单一氮素或高硝态氮混合肥,樟树施肥应以“少硝、铵适量”混合性氮肥为宜。

桧木醇通过TGF-β1/Foxp3/RORγt通路抑制乳腺癌细胞侵袭、迁移及凋亡的影响

目的 研究桧木醇对乳腺癌MCF-7细胞增殖和凋亡的影响及其相关机制。方法 用0、5、10和20μmol/L桧木醇处理人乳腺癌MCF-7细胞,采用划痕法检测细胞的迁移、Transwell法检测细胞侵袭能力、流式细胞技术检测细胞凋亡情况、采用蛋白免疫印迹法检测TGF-β1/Foxp3/RORγt信号通路相关蛋白的表达。结果 与对照组比较,5、10和20μmol/L桧木醇组细胞细胞划痕愈合率、侵袭能力均显著降低(P<0.01或P<0.05),且随着桧木醇浓度的升高,细胞划痕愈合率和侵袭能力逐渐下降,差异有统计学意义(P<0.05);细胞凋亡率显著升高(P<0.01或P<0.05),且随着桧木醇浓度的升高,细胞凋亡率逐渐升高,差异有统计学意义(P<0.05);TGF-β1、Foxp3、RORγt蛋白显著降低(P<0.05),随着桧木醇浓度的升高,TGF-β1、Foxp3、RORγt蛋白表达水平逐渐降低,差异有统计学意义(P<0.05)。结论 桧木醇可以通过抑制乳腺癌细胞中TGF-β1/Foxp3/RORγt信号通路来调节乳腺癌细胞的侵袭、迁移和凋亡,具有成为乳腺癌免疫治疗的潜在靶点。

烟叶香型特征致香成分研究及含量测定

采用高效液相色谱法(HPLC)对烟叶致香成分麦芽酚、乙基麦芽酚、香兰素、芳樟醇、β-大马酮的含量进行分析、鉴定和评价。通过查阅相关文献,确定以麦芽酚、乙基麦芽酚、香兰素、芳樟醇、β-大马酮烟叶特征致香成分为考查对象,在规定色谱条件下对检测方法的精密度、稳定性进行研究,并对湖南永州(2019)陈化烟叶样品中5种特征致香成分含量进行了测定分析。在规定色谱条件下,5种致香物质的特征吸收峰与其他各组分均达到良好的基线分离。建立的麦芽酚、乙基麦芽酚、香兰素、芳樟醇、β-大马酮HPLC测定方法可行。