Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different...Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different boar breeds,the sperm motility,sperm viability and sperm deformity,as well as the spermadhesins' m RNA expression in fresh semen of Luchuan,Landrace and Duroc boars were compared.The results showed that the sperm motility and viability of Luchuan boar showed no significant differences with those of Landrace and Duroc boars(P〉 0.05),but its sperm deformity was significantly lower than those of the other two exotic breeds(P〈0.01);no significant differences were found in the m RNA expression levels of spermadhesins between Luchuan and Landrace,Duroc boars(P〉0.05),excepting that the m RNA expression level of AQN1 in Luchuan boar semen was significantly lower than that in Duroc boar semen(P〈0.05);the m RNA expression levels of AQN3,AWN and PSP-II in Landrace boar semen were significantly higher than those in Duroc boar semen(P〈0.05,P〈0.01).In short,there were significant differences in the m RNA expression levels of spermadhesins among different pig breeds,which showed no significant correlations with the differences in sperm motility,viability and deformity.展开更多
Immunohistochemistry (IHC) and semi-quantitative RT-PCR were used to detect the distribution of GnIH in the central nervous system of 30-day-old male Luchuan piglets, which laid a theoretical basis for in-depth rese...Immunohistochemistry (IHC) and semi-quantitative RT-PCR were used to detect the distribution of GnIH in the central nervous system of 30-day-old male Luchuan piglets, which laid a theoretical basis for in-depth research on physiological functions of Gn[H from the perspective of morphology and molecular lev- el. IHC results showed that in central nervous system, GnIH immunoreactive neurons distributed in cerebral cortex, cerebellum, hippocampus, olfactory bulb, ports, midbrain, medulla oblongata and spinal cord of Luchuan piglets, but GnIH-immunoreactive (GnIH-ir) cell bodies employed diversified morphologies with va- rying sizes. The results of semi-quantitative RT-PCR showed that the expression of GnIH mRNA in medulla obhingata was the highest, followed by midbrain, and the expression of GnIH mRNA in olfactory bulb was the lowest, while those in other tissues were medium. The results of IHC and semi-quantitative RT-PCR were coincident, which further confirmed wide distribution of GnIH in the central nervous system of male Luchuan piglets.展开更多
文摘Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different boar breeds,the sperm motility,sperm viability and sperm deformity,as well as the spermadhesins' m RNA expression in fresh semen of Luchuan,Landrace and Duroc boars were compared.The results showed that the sperm motility and viability of Luchuan boar showed no significant differences with those of Landrace and Duroc boars(P〉 0.05),but its sperm deformity was significantly lower than those of the other two exotic breeds(P〈0.01);no significant differences were found in the m RNA expression levels of spermadhesins between Luchuan and Landrace,Duroc boars(P〉0.05),excepting that the m RNA expression level of AQN1 in Luchuan boar semen was significantly lower than that in Duroc boar semen(P〈0.05);the m RNA expression levels of AQN3,AWN and PSP-II in Landrace boar semen were significantly higher than those in Duroc boar semen(P〈0.05,P〈0.01).In short,there were significant differences in the m RNA expression levels of spermadhesins among different pig breeds,which showed no significant correlations with the differences in sperm motility,viability and deformity.
基金Supported by Student’s Innovation and Entrepreneurship Training Program of National Innovation Training Program(141059332)Fund Project of Guangxi University(XJZ14307)+2 种基金Youth Science Fund Project of National Natural Science Foundation of China(31402151,31502079)Youth Science Fund Project of Natural Science Foundation of Guangxi Province(2015GXNSFBA139077,2015GXNSFBA139067)Innovation and Entrepreneurship Training Program for College Students in Guangxi Province(20151059309,201510593118)
文摘Immunohistochemistry (IHC) and semi-quantitative RT-PCR were used to detect the distribution of GnIH in the central nervous system of 30-day-old male Luchuan piglets, which laid a theoretical basis for in-depth research on physiological functions of Gn[H from the perspective of morphology and molecular lev- el. IHC results showed that in central nervous system, GnIH immunoreactive neurons distributed in cerebral cortex, cerebellum, hippocampus, olfactory bulb, ports, midbrain, medulla oblongata and spinal cord of Luchuan piglets, but GnIH-immunoreactive (GnIH-ir) cell bodies employed diversified morphologies with va- rying sizes. The results of semi-quantitative RT-PCR showed that the expression of GnIH mRNA in medulla obhingata was the highest, followed by midbrain, and the expression of GnIH mRNA in olfactory bulb was the lowest, while those in other tissues were medium. The results of IHC and semi-quantitative RT-PCR were coincident, which further confirmed wide distribution of GnIH in the central nervous system of male Luchuan piglets.