Initial therapeutic evidence of a borosilicate bioactive glass(BSG)and Fe_(3)O_(4)magnetic nanoparticle scaffold on implant-associated Staphylococcal aureus bone infection

Implant-associated Staphylococcus aureus(S.aureus)osteomyelitis is a severe challenge in orthopedics.While antibiotic-loaded bone cement is a standardized therapeutic approach for S.aureus osteomyelitis,it falls short in eradicating Staphylococcus abscess communities(SACs)and bacteria within osteocyte-lacuna canalicular network(OLCN)and repairing bone defects.To address limitations,we developed a borosilicate bioactive glass(BSG)combined with ferroferric oxide(Fe_(3)O_(4))magnetic scaffold to enhance antibacterial efficacy and bone repair capabilities.We conducted comprehensive assessments of the osteoinductive,immunomodulatory,antibacterial properties,and thermal response of this scaffold,with or without an alternating magnetic field(AMF).Utilizing a well-established implant-related S.aureus tibial infection rabbit model,we evaluated its antibacterial performance in vivo.RNA transcriptome sequencing demonstrated that BSG+5%Fe_(3)O_(4)enhanced the immune response to bacteria and promoted osteogenic differentiation and mineralization of MSCs.Notably,BSG+5%Fe_(3)O_(4)upregulated gene expression of NOD-like receptor and TNF pathway in MSCs,alongside increased the expression of osteogenic factors(RUNX2,ALP and OCN)in vitro.Flow cytometry on macrophage exhibited a polarization effect towards M2,accompanied by upregulation of anti-inflammatory genes(TGF-β1 and IL-1Ra)and downregulation of pro-inflammatory genes(IL-6 and IL-1β)among macrophages.In vivo CT imaging revealed the absence of osteolysis and periosteal response in rabbits treated with BSG+5%Fe_(3)O_(4)+AMF at 42 days.Histological analysis indicated complete controls of SACs and bacteria within OLCN by day 42,along with new bone formation,signifying effective control of S.aureus osteomyelitis.Further investigations will focus on the in vivo biosafety and biological mechanism of this scaffold within infectious microenvironment.

Effect of magnetic nanoparticles on magnetic field homogeneity

The mechanism of magnetic nanoparticles(MNPs)affecting magnetic field uniformity is studied in this work.The spatial distribution of MNPs in liquid is simulated based on Monte Carlo method.The induced field of the single MNP is combined with the magnetic field distribution of magnetofluid.In the simulation,magnetic field uniformity is described by a statistical distribution.As the chemical shift(CS)and full width at half maximum(FWHM)of magnetic resonance(MR)spectrum can reflect the uniformity of magnetic field,the simulation is verified by spectrum experiment.Simulation and measurement results prove that the CS and FWHM of the MR spectrum are basically positively correlated with the concentration of MNPs and negatively correlated with the temperature.The research results can explain how MNPs play a role in MR by affecting the uniform magnetic field,which is of great significance in improving the temperature measurement accuracy of magnetic nanothermometers and the spatial resolution of magnetic particle imaging.

Adsorption behavior and adsorption mechanism of Cu(Ⅱ) ions on amino-functionalized magnetic nanoparticles

Amino-functionalized magnetic nanoparticle （NH2-MNP） were prepared by a sol-gel approach. The adsorption behavior of Cu（II） ions on NH2-MNP was discussed systematically by batch experiments. The effects of initial Cu（II） ions concentration, time, pH and temperature were investigated. In kinetic studies, the pseudo-second-order model was successfully employed, and the pseudo-first-order model substantiated that Cu（II） adsorption on NH2-MNP was a diffusion-based process. Langmuir model and Dubinin-Radushkevich model （R2〉0.99） were more corresponded with the adsorption isotherm data of Cu（II） ions than Freundlich model. The adsorption capacity was increased with the increment of temperature and pH. NH2-MNP remains excellent Cu（II） recoveries after reusing five adsorption and desorption cycles, making NH2-MNP a promising candidate for repetitively removing heavy metal ions from environmental water samples. According to the results obtained from adsorption activation energy and thermodynamic studies, it can be inferred that the main adsorption mechanism between absorbent and Cu（II） ions is ion exchange-surface complexation.

Effect of targeted magnetic nanoparticles containing 5-FU on expression of bcl-2, bax and caspase 3 in nude mice with transplanted human liver cancer

AIM: To investigate the anti-tumor effect and mechanisms of magnetic nanoparticles targeting hepatocellular carcinoma. METHODS: Human hepatocellular carcinoma was induced in nude mice, and the mice were randomly divided into group A receiving normal saline, group B receiving magnetic nanoparticles containing 5-fluorouracil (5-FU), group C receiving 5-FU, and group D receiving magnetic nanoparticles containing 5-FU with a magnetic field built in tumor tissues. The tumor volume was measured on the day before treatment and 1, 4, 7, 10 and 13 d after treatment. Tumor tissues were isolated for examination of the expression of bcl-2, bax and caspase 3 by immunohistochemical method, reverse transcription polymerase chain reaction and Western blotting. RESULTS: The tumor volume was markedly lower in groups C and D than in groups A and B (group C or D vs group A or B, P < 0.01). The volume was markedly lower in group D than in group C (P < 0.05). The expression of protein and mRNA of bcl-2 was markedly lower in groups C and D than in groups A and B (group C or D vs group A or B, P < 0.01), and was markedly lower in group D than in group C (P < 0.01). The expression of bax and caspase 3 in groups C and D was signif icantly increased, compared with that in groups A and B (P < 0.01). CONCLUSION: The targeted magnetic nanoparticles containing 5-FU can improve the chemotherapeutic effect of 5-FU against hepatocellular carcinoma by decreasing the expression of bcl-2 gene, and increasing the expression of bax and caspase 3 genes.

Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatocellular carcinoma marker glypican-3

Glypican-3 （GPC3） is reported as a great promising tumor marker for hepatocellular carcinoma （HCC） diagnosis. Highly sensitive and accurate analysis of serum GPC3 （sGPC3）, in combination with or instead of traditional HCC marker alpha-fetoprotein （AFP）, is essential for early diagnosis of I-ICC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles （MnPs） and magnetic microparticles （MmPs） with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay （CLEIA）. After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance.

PREPARATION AND CHARACTERIZATION OF PVA COATED MAGNETIC NANOPARTICLES

Polyvinyl alcohol coated magnetic particles (PVA ferrofluids) have been synthesized by chemical co-precipitation of Fe(II)/Fe(III) salts in 1.5 mol/L NH4OH solution at 70 degreesC in the presence of PVA. The resultant colloidal particles have core-shell structures, in which the iron oxide crystallites form the cores and PVA chains form the shells. The hydrodynamic diameter of the colloidal particles is in the range of 108 to 155 nm, which increases with increasing PVA concentration from 5 wt% to 20 wt%, The size of the magnetic cores is ca. 5-10 nm, which is relatively independent of PVA concentration. Under transmission electron microscopic (TEM) examination, the magnetic cores exhibit somewhat irregular shapes varying from spherical, oval, to cubic. Magnetometry measurement revealed that the PVA coated magnetic particles are superparamagnetic. The saturation magnetization of 5 wt% and 20 wt% PVA ferrofluids at 300 K is 54 and 49 emu/g, respectively. All the PVA ferrofluids exhibited excellent colloidal stability in pure water and phosphate buffer saline (PBS, pH = 7.4). The ferrofluids can remain stable in above solutions for more than three months at 4 degreesC.

Fabrication of Poly（y-glutamic acid）-coated Fe3O4 Magnetic Nanoparticles and Their Application in Heavy Metal Removal

In this study, poly（y-glutamic acid）-coated Fe3O4 magnetic nanoparticles （y-PGA/Fe304 MNPs） were successfully fabricated using the co-precipitation method. Fe3O4 MNPs were also prepared for comparison. The av erage size and specific surface area results reveal that 7-PGA/Fe304 MNPs （52.4 nm, 88.41 m2.g-1） have smaller particle size and larger specific surface area_ than Fe3O4 MNPs （62.0 nm, 76.83 mLg-1）. The y-PGA/Fe3O4 MNPs

Immobilized laccase on magnetic nanoparticles for enhanced lignin model compounds degradation

As a natural aromatic polymer,lignin has great potential but limited industrial application due to its complex chemical structure.Among strategies for lignin conversion,biodegradation has attracted promising interest recently in term of efficiency,selectivity and mild condition.In order to overcome the issues of poor stability and non-reusability of enzyme in the biodegradation of lignin,this work explored a protocol of immobilized laccase on magnetic nanoparticles(MNPs)with rough surfaces for enhanced lignin model compounds degradation.Scanning electron microscope with energy dispersive spectrometer(SEM-EDS),flourier transformation infrared spectroscopy(FTIR)and thermal gravimetric analysis(TGA)were utilized to characterize the immobilization of laccase.The results showed a maximum activity recovery of 64.7%towards laccase when it was incubated with MNPs and glutaraldehyde(GA)with concentrations of 6 mg·ml^-1and 7.5 mg·ml^-1for 5 h,respectively.The immobilized laccase showed improved thermal stability and pH tolerance compared with free laccase,and remained more than 80%of its initial activity after 20 days of storage at 4℃.In addition,about 40%residual activity of the laccase remained after 8 times cycles.Gas chromatography–mass spectrometry(GC–MS)was utilized to characterize the products of lignin model compound degradation and activation,and the efficiency of immobilized laccase was calculated to be 1–5 times that of free laccase.It was proposed that the synergistic effect between MNPs and laccase displays an important role in the enhancement of stability and activity in lignin model compound biodegradation.

Simulation research on effect of magnetic nanoparticles on physical process of magneto–acoustic tomography with magnetic induction

Magneto–acoustic tomography with magnetic induction（MAT-MI） is a multiphysics coupled imaging technique that is combined with electrical impedance tomography and ultrasound imaging. In order to study the influence of adding magnetic nanoparticles as a contrast agent for MAT-MI on its physical process, firstly, we analyze and compare the electromagnetic and acoustical properties of MAT-MI theoretically before and after adding magnetic nanoparticles, and then construct a two-dimensional（2 D） planar model. Under the guidance of space-time separation theory, we determine the reasonable simulation conditions and solve the electromagnetic field and sound field physical processes in the two modes by using the finite element method. The magnetic flux density, sound pressure distribution, and related one-dimensional（1 D）, 2 D, and three-dimensional（3 D） images are obtained. Finally, we make a qualitative and quantitative analysis based on the theoretical and simulation results. The research results show that the peak time of the time item separated from the sound source has a corresponding relationship with the peak time of the sound pressure signal. At this moment, MAMPTMI produces larger sound pressure signals, and the sound pressure distribution of the MAMPT-MI is more uniform, which facilitates the detection and completion of sound source reconstruction. The research results may lay the foundation for the MAT-MI of magnetically responsive nanoparticle in subsequent experiments and even clinical applications.

Magnetic nanoparticle-based cancer therapy

Nanoparticles（NPs） with easily modified surfaces have been playing an important role in biomedicine.As cancer is one of the major causes of death,tremendous efforts have been devoted to advance the methods of cancer diagnosis and therapy.Recently,magnetic nanoparticles（MNPs） that are responsive to a magnetic field have shown great promise in cancer therapy.Compared with traditional cancer therapy,magnetic field triggered therapeutic approaches can treat cancer in an unconventional but more effective and safer way.In this review,we will discuss the recent progress in cancer therapies based on MNPs,mainly including magnetic hyperthermia,magnetic specific targeting,magnetically controlled drug delivery,magnetofection,and magnetic switches for controlling cell fate.Some recently developed strategies such as magnetic resonance imaging（MRI） monitoring cancer therapy and magnetic tissue engineering are also addressed.

Intensification of levofloxacin sono-degradation in a US/H_2O_2 system with Fe_3O_4 magnetic nanoparticles

Fe3O4 magnetic nanoparticles(MNPs) were synthesised, characterised, and used as a peroxidase mimetic to accelerate levofloxacin sono-degradation in an ultrasound(US)/H2O2 system. The Fe3O4 MNPs were in nanometre scale with an average diameter of approximately 12 to 18 nm. The introduction of Fe3O4 MNPs increased levofloxacin sono-degradation in the US/H2O2 system. Experimental parameters, such as Fe3O4 MNP dose, initial solution p H, and H2O2 concentration, were investigated by a one-factor-at-a-time approach. The results showed that Fe3O4 MNPs enhanced levofloxacin removal in the p H range from 4.0 to 9.0. Levofloxacin removal ratio increased with Fe3O4 MNP dose up to 1.0 g·L-1and with H2O2 concentration until reaching the maximum. Moreover, three main intermediate compounds were identified by HPLC with electrospray ionisation tandem mass spectrometry, and a possible degradation pathway was proposed. This study suggests that combination of H2O2, Fe3O4 MNPs and US is a good way to improve the degradation efficiency of antibiotics.

Combined Effects of 50 Hz Magnetic Field and Magnetic Nanoparticles on the Proliferation and Apoptosis of PC12 Cells

Objective To investigate the bioeffects of extremely low frequency （ELF） magnetic field （MF） （50 Hz, 400 μT） and magnetic nanoparticles （MNPs） via cytotoxicity and apoptosis assays on PC12 cells. Methods MNPs modified by SiO2 （MNP-SiO2） were characterized by transmission electron microscopy （TEM）, dynamic light scattering and hysteresis loop measurement. PC12 cells were administrated with MNP-SiO2 with or without MF exposure for 48 h. Cytotoxicity and apoptosis were evaluated with MTI- assay and annexin V-FITC/PI staining, respectively. The morphology and uptake of MNP-SiO2 were determined by TEM. MF simulation was performed by Ansoft Maxwell based on the finite element method. Results MNP-SiO2 were identified as -20 nm （diameter） ferromagnetic particles. MNP-SiO2 reduced cell viability in a dose-dependent manner. MF also reduced cell viability with increasing concentrations of MNP-SiO2. MNP-SiO2 alone did not cause apoptosis in PC12 cells; instead, the proportion of apoptotic cells increased significantly under MF exposure and increasing doses of MNP-SiO2. MNP-SiO2 could be ingested and then cause a slight change in cell morphology. Conclusion Combined exposure of MF and MNP-SiO2 resulted in remarkable cytotoxicity and increased apoptosis in PC12 cells. The results suggested that MF exposure could strengthen the MF of MNPs, which may enhance the bioeffects of ELF MF.

Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles （MSNP） prepared by two steps, i.e., Fe304 synthesis and silica shell growth on the surface. This magnetic nanoparticle supported ionic liquid （MNP-IL） were applied in the immobilization of penicillin G acylase （PGA）. The MSNPs and MNP-ILs were characterized by themeans of Fourier transform infrared spectroscopy （FTIR）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, and vibrating sample magnetometer （VSM）. The results showed that the average size of magnetic Fe304 nanoparticles and MSNPs were -10 and -90 nm, respectively. The saturation magnetizations of magnetic Fe304 nanoparticles and MNP-ILs were 63.7 and 26.9 A＇m2·kg^-1, respectively. The MNP-IL was successfully applied in the immobilization of PGA. The maximum amount of loaded enzyme-was about 209 mg·g^-1 （based on carder）, and the highest enzyme activity of immobilized PGA （based on ImPGA） was 261 U·g^-1. Both the amount of loaded enzyme and the activity of ImPGA are at the same leyel of or higher than that in previous reports. After 10 consecutive operat!ons, ImPGA still mainrained 62% of its initial activity, indicating the＇good recovery property of ImPGA activity. The ionic liquid modified magnetic particles integrate the magnetic properties of Fe304 and the structure-tunable properties of ionic liquids, and have extensive potential uses in protein immobilization and magnetic bioseparation. This work may open up a novel strategy to immobilize proteins by ionic liquids.

Surface modification of magnetic nanoparticles in biomedicine

Progress in surface modification of magnetic nanoparticles（MNPs） is summarized with regard to organic molecules,macromolecules and inorganic materials. Many researchers are now devoted to synthesizing new types of multi-functional MNPs, which show great application potential in both diagnosis and treatment of disease. By employing an ever-greater variety of surface modification techniques, MNPs can satisfy more and more of the demands of medical practice in areas like magnetic resonance imaging（MRI）, fluorescent marking, cell targeting, and drug delivery.

Functionalized magnetic nanoparticles for drug delivery in tumor therapy

The side effects of chemotherapy are mainly the poor control of drug release. Magnetic nanoparticles(MNPs) have super-paramagnetic behaviors which are preferred for biomedical applications such as in targeted drug delivery, besides, in magnetic recording, catalysis, and others. MNPs, due to high magnetization response, can be manipulated by the external magnetic fields to penetrate directly into the tumor, thus they can act as ideal drug carriers. MNPs also play a crucial role in drug delivery system because of their high surface-to-volume ratio and porosity. The drug delivery in tumor therapy is related to the sizes, shapes, and surface coatings of MNPs as carriers. Therefore, in this review, we first summarize the effects of the sizes, shapes, and surface coatings of MNPs on drug delivery, then discuss three types of drug release systems, i.e., p H-controlled, temperature-controlled, and magnetic-controlled drug release systems, and finally compare the principle of passive drug release with that of active drug release in tumor therapy.

Multifunctional magnetic nanoparticles for magnetic resonance image-guided photothermal therapy for cancer

Key advances in multifunctional magnetic nanoparticles （MNPs） for magnetic resonance （MR） image-guided pho- tothermal therapy of cancer are reviewed. We briefly outline the design and fabrication of such multifunctional MNPs. Bimodal image-guided photothermal therapies （MR/fluorescence and MR/ultrasound） are also discussed.

Impacts of Heat Flux Distribution, Sloping Magnetic Field and Magnetic Nanoparticles on the Natural Convective Flow Contained in a Square Cavity

In the present paper,the effect of the heat flux distribution on the natural convective flow inside a square cavity in the presence of a sloping magnetic field and magnetic nanoparticles is explored numerically.The nondimensional governing equations are solved in the framework of a finite element method implemented using the Galerkin approach.The role played by numerous model parameters in influencing the emerging thermal and concentration fields is examined;among them are:the location of the heat source and its lengthH
,the magnitude of the thermal Rayleigh number,the nanoparticles shape and volume fraction,and the Hartmann number.It is found that the nanofluid velocity becomes higher when the thermal source length,the nanoparticles volume fraction and/or the thermal Rayleigh number are increased,while it decreases as the Hartmann number Ha grows and the position of the heat source moves toward the center of the lower wall of the cavity.Moreover,the temperature of the nanofluid grows with the extension of the thermal source and decreases slowly when the heat flux position moves toward the center of the lower wall.The outcomes of the research also indicate that the average Nusselt number becomes smaller on increasing Hartmann number Ha and heat source length H^(*).The addition of Fe_(3)O_(4) to engine oil leads to a higher rate of heat transfer with respect to the addition of SiO_(2) particles.Blade-shaped nanoparticles generate the highest value of the Nusselt number compared to all the other considered shapes.

A magnetic nanoparticles-based method for DNA extraction from the saliva of stroke patients

C677T polymorphism in the methylenetetrahydrofolate reductase （MTHFR） gene is a risk factor for stroke, suggesting that widespread detection could help to prevent stroke. DNA from 70 stroke pa- tients and 70 healthy controls was extracted from saliva using a magnetic nanoparticles-based method and from blood using conventional methods. Real-time PCR results revealed that the C677T polymorphism was genotyped by PCR using DNA extracted from both saliva and blood samples. The genotype results were confirmed by gene sequencing, and results for saliva and blood samples were consistent. The mutation TT genotype frequency was significantly higher in the stroke group than in controls. Homocysteine levels were significantly higher than controls in both TT genotype groups. Therefore, this noninvasive magnetic nanoparticles-based method using saliva samples could be used to screen for the MTHFR C677T polymorphism in target populations.

Modification of Fe_3O_4 Magnetic Nanoparticles by L-dopa or Dopamine as an Enzyme Support

Fe3O4 magnetic nanoparticles were prepared by co-precipitation of Fe^2＋ and Fe^3＋ in an ammonia solution, and its size was about 36 nm measured by an atomic force microscope. Fe3O4 magnetic nanoparticles were modified by L-dopa or dopamine using sonication method. The analysis of FTIR clearly indicated the formation of Fe-O-C bond. Direct immobilization of trypsin （EC： 3.4.21.4） on Fe3O4 magnetic nanoparticles with L-dopa and dopamine spacer was investigated using glutaraldehyde as a coupling agent. No significant changes in the size and magnetic property of the three kinds of magnetic nanoparticles linked with or without trypsin were observed. The existence of the spacer molecule on magnetic nanoparticles could greatly improve the activity and the storage stability of bound trypsin through increasing the flexibility of enzyme and changing the microenvironment on nanoparticles surface compared to the naked magnetic nanoparticles.

Synthesis of ternary magnetic nanoparticles for enhanced catalytic conversion of biomass-derived methyl levulinate into γ-valerolactone

Conversion of levulinic acid and its esters into versatile y-valerolactone(GVL)is a pivotal and challenging step in biorefineries,limited by high catalyst cost,the use of hydrogen atmosphere,or tedious catalyst preparation and recycling process.Here we have successfully synthesized a ternary magnetic nanoparticle catalyst(Al_(2)O_(3)-ZrO_(2)/Fe_(3)O_(4)(5)),over which biomass-derived methyl levulinate(ML)can be quantitively converted to GVL with an extremely high selectivity of>99%and yield of-98%in the absence of molecular hydrogen.Al_(2)O_(3)-ZrO_(2)/Fe_(3)O_(4)(5)incorporates simultaneously inexpensive alumina and zirconia onto magnetite support by a facile coprecipitation method,giving rise to a core-shell structure,welldistributed acid-base sites,and strong magnetism,as evidenced by the X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM),transmission electron microscopy(TEM),high-angle annular dark-field scanning-TEM(HAADF-STEM),SEM-energy dispersive Xray spectroscopy(SEM-EDX),temperature-programmed desorption of ammonia(NH3-TPD),temperature-programmed desorption of carbon dioxide(CO_(2)-TPD),pyridine-adsorption infrared spectra(Py-IR),and vibrating sample magnetometry(VSM).Such characteristics enable it to be highly active and easily recycled by a magnet for at least five cycles with a slight loss of its catalytic activity,avoiding a time-consuming and energy-intensive reactivation process.It is found that there was a synergistic effect among the metal oxides,and the high efficiency and selectivity originating from such synergism are evidenced by kinetic studies.Furthermore,a reaction mechanism regarding the hydrogenation of ML to GVL is proposed by these findings,coupled with gas chromatography-mass spectrometry(GC-MS)analysis.Accordingly,this readily synthesized and recovered magnetic nanocatalyst for conversion of biomassderived ML into GVL can provide an eco-friendly and safe way for biomass valorization.