AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal ci...AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.展开更多
pH-sensitive liposomes are designed to specifically triggered release the loaded drugs in response to the change of pH in the surrounding serum.So pH-sensitive liposomes can effectively deliver drug or gene fragments ...pH-sensitive liposomes are designed to specifically triggered release the loaded drugs in response to the change of pH in the surrounding serum.So pH-sensitive liposomes can effectively deliver drug or gene fragments into the cytoplasm via the endocytotic pathway.Furthermore,pH-sensitive liposomes can be successfully used in clinical if they enable the encapsulated drugs to be targeted to pathological tissues(such as primary tumors,metastases,local ischemia,inflammation and infection)of the body in which pH is less than the normal physiological value.That’s the reason why a growing amount of literatures described the development and applications of pH-sensitive liposomes to improve the therapeutic index of the encapsulated active ingredients.In this review,the commonly used pH-sensitive molecules for pH-sensitive liposome and the mechanisms of intracellular delivery of pH-sensitive liposomes were addressed.Besides,the potential clinical applications were fully discussed in detail with an expectation to contribute to the clinical research of pH-sensitive liposomes.展开更多
To evaluate the feasibility of using magnetic iron oxide nanoparticle as wild PTEN gene carrier for transfection in vitro to reverse cisplatin-resistance of A549/CDDP cells, A549/CDDP cells were transfected with the w...To evaluate the feasibility of using magnetic iron oxide nanoparticle as wild PTEN gene carrier for transfection in vitro to reverse cisplatin-resistance of A549/CDDP cells, A549/CDDP cells were transfected with the wild PTEN gene expression plasmid (pGFP-PTEN) by magnetic iron nanoparticle and lipo2000. The transfection efficiency was detected by fluorescence microscope and flow cytometer. The expression levels of PTEN mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry analysis. The effect of PTEN transfection on cell cycle enhances the sensitivity of A549/CDDP to cisplatin and nanoparticle-mediated transfection has a higher efficiency than that of the liposome-mediated group. The apoptosis level was up-regulated in PTEN transfection group. The magnetic iron oxide nanoparticle could be used as one of the ideal gene carriers for PTEN gene delivery in vitro. PTEN can be an effective target for reversing cisplatin-resistance in lung cancer.展开更多
为了从金钱菇中快速分离出降血压肽,利用超声细胞破碎法提取金钱菇蛋白,采用透析、超滤的方法对提取物进行纯化,以固定化金属亲和磁性脂质体(metal affinity-immobilied magnetic liposome,MA-IML)作为吸附介质,以金钱菇提取物为吸附对...为了从金钱菇中快速分离出降血压肽,利用超声细胞破碎法提取金钱菇蛋白,采用透析、超滤的方法对提取物进行纯化,以固定化金属亲和磁性脂质体(metal affinity-immobilied magnetic liposome,MA-IML)作为吸附介质,以金钱菇提取物为吸附对象,吸附分离其中的降血压肽,并解析其结构,利用分子对接分析其结合血管紧张素I转化酶(angiotensin-I converting enzyme,ACE)的能力。结果表明:对金钱菇提取液用透析、超滤的方法进行纯化后,蛋白含量由37.67%提高至51.18%,多糖含量由26.80%降低至6.90%,ACE抑制率由61.83%提高至79.77%,纯化后更有利于分离降血压肽;用MA-IML粒子吸附分离出金钱菇纯化液中的降血压活性组分,经反相高效液相色谱法分析其为单一组分,该组分为五肽,其氨基酸序列为苯丙氨酸-丙氨酸-色氨酸-丙氨酸-酪氨酸(Phe-Ala-Trp-Ala-Tyr,FAWAY),IC50为179μmol/L。分子对接模拟表明,FAWAY能与ACE自发发生结合并形成稳定的构象。该研究制备的MA-IML可作为从金钱菇中分离降血压肽的有效工具。展开更多
Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)...Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)-based strategy for detecting S.aureus in complex samples via the assistance of signal amplifi cation system of liposome.In this design,vancomycin(Van)modifi ed 200 nm magnetic beads(MB_(200)-Van)and biotinylated immunoglobulin(Biotin-IgG)were employed as capture probes for S.aureus which following labelled by biotinylated liposomes loading with glucose molecule(Biotinliposome@Glu,BLGs)based on the affi nity of biotin with streptavidin.The released glucose molecules combining with glucose oxidase generated Fe^(3+),causing transverse relaxation time(T_(2))signal change.The combination of dual recognition of S.aureus,liposome assisted signal amplifi cation,and the MRS-based assay achieved direct and specifi c detection of S.aureus in blood and abscess samples.The as-proposed method for S.aureus assay in this study shows potential application for infectious diagnosis in clinical.展开更多
文摘AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.
文摘pH-sensitive liposomes are designed to specifically triggered release the loaded drugs in response to the change of pH in the surrounding serum.So pH-sensitive liposomes can effectively deliver drug or gene fragments into the cytoplasm via the endocytotic pathway.Furthermore,pH-sensitive liposomes can be successfully used in clinical if they enable the encapsulated drugs to be targeted to pathological tissues(such as primary tumors,metastases,local ischemia,inflammation and infection)of the body in which pH is less than the normal physiological value.That’s the reason why a growing amount of literatures described the development and applications of pH-sensitive liposomes to improve the therapeutic index of the encapsulated active ingredients.In this review,the commonly used pH-sensitive molecules for pH-sensitive liposome and the mechanisms of intracellular delivery of pH-sensitive liposomes were addressed.Besides,the potential clinical applications were fully discussed in detail with an expectation to contribute to the clinical research of pH-sensitive liposomes.
基金Project(07JJ3055)supported by the Natural Science Foundation of Hunan Province,China
文摘To evaluate the feasibility of using magnetic iron oxide nanoparticle as wild PTEN gene carrier for transfection in vitro to reverse cisplatin-resistance of A549/CDDP cells, A549/CDDP cells were transfected with the wild PTEN gene expression plasmid (pGFP-PTEN) by magnetic iron nanoparticle and lipo2000. The transfection efficiency was detected by fluorescence microscope and flow cytometer. The expression levels of PTEN mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry analysis. The effect of PTEN transfection on cell cycle enhances the sensitivity of A549/CDDP to cisplatin and nanoparticle-mediated transfection has a higher efficiency than that of the liposome-mediated group. The apoptosis level was up-regulated in PTEN transfection group. The magnetic iron oxide nanoparticle could be used as one of the ideal gene carriers for PTEN gene delivery in vitro. PTEN can be an effective target for reversing cisplatin-resistance in lung cancer.
基金supported by the National Natural Science Foundation of China(22174116,21974110)Chongqing Science Funds for Distinguished Young Scientists(cstc2021jcyjjqx0024)the Innovation Research Group at higher Education Institutions in Chongqing,Chongqing Education Committee(CXQT21006)
文摘Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)-based strategy for detecting S.aureus in complex samples via the assistance of signal amplifi cation system of liposome.In this design,vancomycin(Van)modifi ed 200 nm magnetic beads(MB_(200)-Van)and biotinylated immunoglobulin(Biotin-IgG)were employed as capture probes for S.aureus which following labelled by biotinylated liposomes loading with glucose molecule(Biotinliposome@Glu,BLGs)based on the affi nity of biotin with streptavidin.The released glucose molecules combining with glucose oxidase generated Fe^(3+),causing transverse relaxation time(T_(2))signal change.The combination of dual recognition of S.aureus,liposome assisted signal amplifi cation,and the MRS-based assay achieved direct and specifi c detection of S.aureus in blood and abscess samples.The as-proposed method for S.aureus assay in this study shows potential application for infectious diagnosis in clinical.
