Accuracy verification methodology for computer-generated hologram used for testing a 3.5-meter mirror based on an equivalent element

Interferometry with computer-generated holograms(CGHs)is a unique solution for the highly accurate testing of large-aperture aspheric mirrors.However,no direct testing method for quantifying the measurement accuracy of CGHs has been developed.In this study,we developed a methodology for verifying CGH accuracy based on an element that is functionally equivalent to a large-aperture mirror in terms of accuracy verification.The equivalent element decreased the aperture by one or higher orders of magnitude,implying that the mirror could be replaced by a non-CGH technology in a comparison test.In this study,a 281 mm diamond-turned mirror was fabricated as the equivalent element of a 3.5 m aspheric mirror and measured using CGH and LUPHOScan profilometers.Surface error composition and root-mean-square(RMS)density analyses were performed.The methodology verification accuracy of the CGH was 4 nm(RMS)in the low-to mid-frequency bands,with a measured surface accuracy of approximately 10 nm(RMS).This methodology provides a feasible solution for CGH accuracy verification,ensuring high-accuracy and reliable testing of large-aperture aspheric mirrors.

Development of a surface plasmon resonance biosensor for accurate and sensitive quantitation of small molecules in blood samples

Therapeutic drug monitoring(TDM)has played an important role in clinical medicine for precise dosing.Currently,chromatographic technology and immunoassay detection are widely used in TDM and have met most of the needs of clinical drug therapy.However,some problems still exist in practical applications,such as complicated operation and the influence of endogenous substances.Surface plasmon resonance(SPR)has been applied to detect the concentrations of small molecules,including pesticide residues in crops and antibiotics in milk,which indicates its potential for in vivo drug detection.In this study,a new SPR-based biosensor for detecting chloramphenicol(CAP)in blood samples was developed and validated using methodological verification,including precision,accuracy,matrix effect,and extraction recovery rate,and compared with the classic ultra-performance liquid chromatographyultraviolet(UPLC-UV)method.The detection range of SPR was 0.1-50 ng/mL and the limit of detection was 0.099±0.023 ng/mL,which was lower than that of UPLC-UV.The intra-day and inter-day accuracies of SPR were 98%-114% and 110%-122%,which met the analysis requirement.The results show that the SPR biosensor is identical to UPLC-UV in the detection of CAP in rat blood samples;moreover,the SPR biosensor has better sensitivity.Therefore,the present study shows that SPR technology can be used for the detection of small molecules in the blood samples and has the potential to become a method for therapeutic drug monitoring.

Microbial Limit Test of Zhideke Granules

[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules.