【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern...【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern杂交定位,对其中一株菌CY32进行全基因测序和生物信息学分析。【结果】5株奇异变形杆菌携带的bla_(CMY-2)位于染色体,其中,菌株CY12、CY32、S31和S52携带floR,菌株CY12和CY32携带qnrD。CY32的染色体同时含有SXT/R391型整合性接合元件(integrative and conjugative elements,ICEs)(ICEPmiJpn1)和PmGRI1共2种耐药基因岛。ICEs的可变区包含2个串联的复合型转座子(IS10构成),其中一个复合型转座子携带bla_(CMY-2);CY32的PmGRI1耐药岛含有12个耐药基因。与其他奇异变形杆菌携带的PmGRI1相比,多重耐药区差异最大的区域位于Tn21转座子。此外,CY32包含2个质粒,包括携带floR的IncQ质粒和携带qnrD的非接合质粒。奇异变形杆菌CY32携带15个耐药基因,呈现多重耐药的特性。【结论】奇异变形杆菌经基因岛和质粒获得多重耐药,使治疗奇异变形杆菌感染变得更加困难,应加强对动物源奇异变形杆菌耐药性监测。展开更多
In this study,the relationship of tropical cyclone(TC)size change rate(SCR),within 24 hours,with size,intensity,and intensity change rate(ICR)are explored over the western North Pacific.TC size is defined as the azimu...In this study,the relationship of tropical cyclone(TC)size change rate(SCR),within 24 hours,with size,intensity,and intensity change rate(ICR)are explored over the western North Pacific.TC size is defined as the azimuthally averaged radius of gale-force wind of 17 m s−1(R17)based on the Multiplatform Tropical Cyclone Surface Winds Analysis data.The majority of SCRs are mainly distributed in the range from−20 to 80 km d−1.The correlation coefficients between SCR and size(SCR-R17),intensity,and ICR(SCR-ICR)are−0.43,−0.12,and 0.25,respectively.The sensitivity of the SCR-R17 and SCR-ICR relationships to size,intensity,and evolution stage are further examined.Results show that the SCR-R17 relationship is more sensitive to variations of size and evolution stage than that of intensity.The relationship of SCR-ICR is largely modulated by the evolution stage.The correlation coefficient of SCR-ICR can increase from 0.25 to 0.40 when only considering the lifetime stages concurrently before and after the lifetime maximum size(LMS)and lifetime maximum intensity.This demonstrates that ICR is a potential factor in predicting SCR during these evolution stages.Besides,the TC size expansion(shrinkage)is more likely to occur for TCs with smaller(larger)size and weaker(stronger)intensity.The complexity of size change during a TC's lifetime can be attributed to the fact that shrinkage or expansion could occur both before and after LMS.展开更多
The rapid development of information technology promotes the transformation and development of future air combat,frommechanization to informatization,intelligence,and multiplatform integration.For the multiplatform av...The rapid development of information technology promotes the transformation and development of future air combat,frommechanization to informatization,intelligence,and multiplatform integration.For the multiplatform avionics system in the unmanned aerial vehicle(UAV)-based network,we aim to address the data routing and sharing issues and propose an integrated communication effectiveness metric.The proposed integrated communication effectiveness is a hierarchical metric consisting of link effectiveness,node effectiveness,and data effectiveness.The link quality,link stability,node honesty,node ability,and data value are concurrently taken into account.We give the normal mathematical expression for the integrated communication effectiveness.We propose a hop-by-hop routing scheme based on a Q-learning algorithm considering the proposed effectiveness metric.Simulation results demonstrate that the proposed scheme is able to find the most efficient routing in the UAV network.展开更多
文摘【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern杂交定位,对其中一株菌CY32进行全基因测序和生物信息学分析。【结果】5株奇异变形杆菌携带的bla_(CMY-2)位于染色体,其中,菌株CY12、CY32、S31和S52携带floR,菌株CY12和CY32携带qnrD。CY32的染色体同时含有SXT/R391型整合性接合元件(integrative and conjugative elements,ICEs)(ICEPmiJpn1)和PmGRI1共2种耐药基因岛。ICEs的可变区包含2个串联的复合型转座子(IS10构成),其中一个复合型转座子携带bla_(CMY-2);CY32的PmGRI1耐药岛含有12个耐药基因。与其他奇异变形杆菌携带的PmGRI1相比,多重耐药区差异最大的区域位于Tn21转座子。此外,CY32包含2个质粒,包括携带floR的IncQ质粒和携带qnrD的非接合质粒。奇异变形杆菌CY32携带15个耐药基因,呈现多重耐药的特性。【结论】奇异变形杆菌经基因岛和质粒获得多重耐药,使治疗奇异变形杆菌感染变得更加困难,应加强对动物源奇异变形杆菌耐药性监测。
基金This study was supported by the National Natural Science Foundation of China[grant numbers 41975071 and 41775063].
文摘In this study,the relationship of tropical cyclone(TC)size change rate(SCR),within 24 hours,with size,intensity,and intensity change rate(ICR)are explored over the western North Pacific.TC size is defined as the azimuthally averaged radius of gale-force wind of 17 m s−1(R17)based on the Multiplatform Tropical Cyclone Surface Winds Analysis data.The majority of SCRs are mainly distributed in the range from−20 to 80 km d−1.The correlation coefficients between SCR and size(SCR-R17),intensity,and ICR(SCR-ICR)are−0.43,−0.12,and 0.25,respectively.The sensitivity of the SCR-R17 and SCR-ICR relationships to size,intensity,and evolution stage are further examined.Results show that the SCR-R17 relationship is more sensitive to variations of size and evolution stage than that of intensity.The relationship of SCR-ICR is largely modulated by the evolution stage.The correlation coefficient of SCR-ICR can increase from 0.25 to 0.40 when only considering the lifetime stages concurrently before and after the lifetime maximum size(LMS)and lifetime maximum intensity.This demonstrates that ICR is a potential factor in predicting SCR during these evolution stages.Besides,the TC size expansion(shrinkage)is more likely to occur for TCs with smaller(larger)size and weaker(stronger)intensity.The complexity of size change during a TC's lifetime can be attributed to the fact that shrinkage or expansion could occur both before and after LMS.
文摘The rapid development of information technology promotes the transformation and development of future air combat,frommechanization to informatization,intelligence,and multiplatform integration.For the multiplatform avionics system in the unmanned aerial vehicle(UAV)-based network,we aim to address the data routing and sharing issues and propose an integrated communication effectiveness metric.The proposed integrated communication effectiveness is a hierarchical metric consisting of link effectiveness,node effectiveness,and data effectiveness.The link quality,link stability,node honesty,node ability,and data value are concurrently taken into account.We give the normal mathematical expression for the integrated communication effectiveness.We propose a hop-by-hop routing scheme based on a Q-learning algorithm considering the proposed effectiveness metric.Simulation results demonstrate that the proposed scheme is able to find the most efficient routing in the UAV network.
